大肠杆菌不同菌株基因组DNA的多态性分析

从大肠杆菌K12菌株JM109基因组克隆了两段DNA重复序列,长度为0.9和0.6kb,分别命名为ECR-1和ECR-6。以ECR-1和ECR-6重复序列作DNA多态性分析的探针,可以鉴别大肠杆菌非常相近的菌株。表明ECR-1和ECR-6 DNA序列可用于大肠杆菌菌株的分类、流行病学和微生态学研究以及大肠杆菌各种致病菌株的临床诊断。     

毛竹（Phyllostachys pubescens）竹笋群动态的研究

 本文从克隆植物生长的调节与适应的角度,研究了毛竹竹笋群的时空动态。竹笋的单位面积产量及个体大小在年际之间变化很大。环境有利的年份出土的竹笋要比条件恶劣的年份多得多、高得多、壮得多。而且,自始至终较早的同生群的竹笋总比较晚的同生群的要高得多、壮得多。较早的同生群的竹笋刚出土时要经历一段生长缓慢的时期,而较晚的同生群的竹笋一出土生长就十分迅速。不管竹笋何时出土,它们几乎都同时进入最快生长期。竹笋高度日增量最快时可达每天100cm左右。本文解释了亚热带竹子在春季不同时间出土的竹笋为何及如何几乎同时抵达林冠顶部。最后,提出了一些有利于竹林经营管理的建议。     

The Expression of Nerve Growth Factor Receptor on Schwann Cells and the Effect of These Cells on Regeneration of Axons in Traumatically Injured Human Spinal Cord

Ｔｈｅ　Ｅｘｐｒｅｓｓｉｏｎ　ｏｆ　Ｎｅｒｖｅ　Ｇｒｏｗｔｈ　Ｆａｃｔｏｒ　Ｒｅｃｅｐｔｏｒ　ｏｎ　Ｓｃｈｗａｎｎ　Ｃｅｌｌｓ　ａｎｄ　ｔｈｅ　Ｅｆｆｅｃｔ　ｏｆ　Ｔｈｅｓｅ　Ｃｅｌｌｓ　ｏｎ　Ｒｅｇｅｎｅｒａｔｉｏｎ　ｏｆ　Ａｘｏｎｓ　ｉｎ　Ｔｒａ...     

越南和泰国横脊叶蝉亚科四新种记述(同翅目:叶蝉科)(英)

本文记述采自越南和泰国的横脊叶蝉亚科Evacanthinae 4新种,即黑斑脊额叶蝉Carinata nigropictura sp. nov., 白脉楔叶蝉Cunedda albovenosa sp. nov., 斑翅楔叶蝉Cunedda signuma sp. nov., 越南翘头叶蝉Dussana vietnama sp. nov.。文中详细描述新种的外部形态和雄性外生殖器构造特征,并与近似种相比较区别,附主要特征图。 模式标本保存在英国自然历史博物馆,其中黑斑脊额叶蝉2副模标本存放在贵州农学院。     

Characterization of progenies of Triticum aestivum- Psathyrostachys juncea derivatives by using genomic in-situ hybridization

Using genomic in-situ hybridization (GISH) technique, 7 translocation-addition lines, 6 transloca-tion and translocation-addition lines, 2 ditelosomic addition lines and 1 translocation line were identified from Triticum aestivum L.-Psathyrostachys juncea (Fisch. ) Nevski intergeneric hybrids, of which translocation-addition and translocation and translocation-addition lines were not found in other reports. No substitutions and disomic additions were detected in the hybrids and breakages occurred in all P. juncea chromosomes studied. Results have shown that the improved GISH technique is a rapid and economical method for use in this field.     

中国汉族人群原纤维蛋白-1基因(FBN1)第27内含子G/A多态性

采用PCR-ASO方法, 对日本筑波大学101名中国汉族留学人员的DNA样品进行了原纤维蛋白-1基因(FBN1)第27内含子G/A多态性测定。结果发现,A等位基因频率为0.5396,G等位基因频率为0.4604。与Tynan等人报道的数据(A和G等位基因的频率分别为0.1675和0.8325)相比,有非常显著差异(P<0 .01),提示两样本间有不同的遗传背景。 Abstract:The G/A polymorphisms in intron 27 of fibrillin-1 gene in 101 Chinese Hans who were studying and working in University of Tsukuba,Japan were analyzed with PCR-ASO method.The frequencies of A and G alleles,were 0.5396 and 0.4604 respectively.In a population sample reported by Tynan the frequency of A allele was 0.1675 that of G allele was 0.8325.The distribution of G/A polymorphism was significantly different between the two population sample(P<0.01),suggesting different genetic backgrounds.     

温泉蛇的染色体组型及Ag-NORs的研究

本文首次报道了温泉蛇(Thermophis baileyi)的染色体组型及Ag-NORs,其核型模式可表达为2n=36=14M+2T+20m, ZW型性决定,Z和W分别为中及亚端部着丝点染色体。该结果与游蛇属种类的核型有较大差异,从而旁证了将温泉蛇从游蛇属中分出的观点。仅一对NOR于No.9染色体长臂并与其次溢痕位置相当,该NOR与锦蛇属种类一致而与游蛇类不同。根据染色体特征,认为温泉蛇在游蛇亚科中处于较为原始的地位。     

大肠杆菌pheA基因在黄色短杆菌中的克隆与表达

在大肠杆菌中,影响苯丙氨酸生物合成的关键酶基因之一是ｐｈｅＡ基因。利用大肠杆菌棒状杆菌穿梭表达载体Ｐｌｃｘ３６克隆了一个对反馈抑制不敏感的大肠杆菌基因ｐｈｅＡ,组建成质粒Ｐｌｃｘ３７。Ｐｌｃｘ３７以接合转移方法转移到抗氟代苯丙氨酸的黄色短杆菌３６２１等菌株中,获得若干基因工程菌株。经发酵测定,编号为３６２１０９的黄色短杆菌的苯丙氨酸产量比出发菌３６２１高出１倍。最高达２６.２６ｍｇ／ｍｌ。