鱼类的天然雌核发育

1.孤雌生殖,雌核发育和杂合发育单性型种群在脊椎动物中是罕见的。尽管如此,到目前为止几乎已在各类脊椎动物中发现了单性型种群。该种群通常涉及3种繁殖方式,即孤雌生殖(parthenogenesis)、雌核发育(gynogenesis)和杂合发育(hybridogenesis)。     

赤点石斑鱼仔,稚幼鱼的发育

本文介绍了以人工繁殖所获的赤点石斑鱼受精卵进行孵化培育的结果,对该种鱼的后期仔鱼、稚、幼鱼的发育作了描述。从孵化的仔鱼发育至完成变态的稚鱼,共需时32天。赤点石斑鱼仔鱼的发育具有很长的背棘和腹棘,第二背棘的最大长度比率约为总体长的50％。仔鱼从8.75毫米长到34.10毫米期间,平均每天可增长1.69毫米的长度。初孵仔鱼全长为1.50毫米,长到144天的幼鱼全长达114.50毫米。     

油菜田三突花蛛的生物学特性初报

通过大田观察和室内饲养,发现武汉地区油菜田三突花蛛3月中旬开始出来活动,4月中旬达到高峰期,主要隐蔽于曲菜花丛中,以待猎物到来,不结网,有飞航习性,数量达225头/百株,占总捕食性天敌的82％,同时开始交配、产卵。不仅与蚜虫、叶蝉的发生正相关,也和油菜的长势呈现正相关性,并对害虫起到明显的控制作用。可见,是油菜田害虫的重要天敌。     

中国林蛙地模标本染色体组型、C带和银带研究

本文报道中国林蛙地模标本的染色体组型、C带和银带。选用染色单体未分开的早中期分裂相制作C带可显示出较多带纹。研究结果表明,中国林蛙与亲缘关系较近的欧洲林蛙在染色体组型、C带和银带上均存在显著差异。再染色体进化上,欧洲林蛙比较原始,中国林蛙比较进化。     

小鼠巨核系祖细胞增殖,分化特性的研究

小鼠骨髓细胞经7d培养后进行细胞形态学观察,可见不同发育阶段的巨核细胞及不同大小的巨核细胞集落。通过计数每个集落中的细胞数,可确定相应祖细胞的有丝分裂能力。结果表明,具有不同有丝分裂能力的祖细胞的体外增殖动力学有所不同。祖细胞的数量与其有丝分裂次数呈负相关(r=-0.986)。进行0、1、2和3次有丝分裂的祖细胞的阿糖胞苷自杀率分别为48.9,58.7,48.0和41.2％;放射敏感性的D_O值(Gy)分别为1.71,1.24,1.03和0.77,D_O值的大小与有丝分裂次数呈负相关(r=-0.958)。经3Gy全身照射后CFU-Meg与CFU-GM的恢复动态过程具有不同特点。     

Construction and packaging of pseudotype retrovirus containing human N—ras cDNA antisense sequence and its biological effects on human hepatoma cells

N-ras is one of the transforming genes in human hepatic cancer cells.It has been found that N-ras was overexpressed at the mRNA and protein level in hepatoma cells.In order to explore the biological roles of N-ras in human hepatic carcinogenesis and the potential application in control of cancer cell growth,a preudotype retrovirus containing antisense sequence of human N-ras was constructed and packaged.A recombinant retrovirus vector containing antisense or sense sequences of N-ras cDNA was constructed by pZIP-NeoSV(X)1.The pseudotype virus was packaged ang rescued by transfection and infection in PA317 and ψ 2 helper cells.It has been demonstrated that the pseudotype retrovirus containing antisense N-ras sequence did inhibit the growth of human PLC/PRF/5 hepatoma cells accompanied with inhibition of p21 expression,while the retrovirus containing sense sequence had none.The pseudotype virus had no effect on human diploid fibroblasts.