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皖南尖吻蝮蛇出血毒素生物学活性与圆二色性关系的研究 总被引:4,自引:0,他引:4
用远紫外CD谱研究了皖南尖吻蝮蛇蛇毒3个出血毒素AaHⅠ、AaHⅢ和AaHⅣ的溶液构象,AaHⅠ的α螺旋、β折叠、β转角和无规卷曲的含量分别为25.8%、12.7%、26.8%和34.7%;AaHⅢ的二级结构含量分别为23.9%、20.6%、23.7%和31.8%;而AaHⅣ分别为18.2%、31.0%、17.2%和33.6%。当pH小于4.0或pH大于11.0时,3种出血毒素α螺旋减少而β折叠增多,同时3种出血毒素的酪蛋白水解活性显著下降。EDTA抑制酪蛋白水解活性,表明3种出血毒素均是金属蛋白酶。EDTA、Cu2+、Zn2+、Ca2+和Mg2+能改变3种出血毒素的二级结构,并影响酪蛋白水解活性。 相似文献
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Crystal structure determination of alkaline haemorrhagin AaHⅢ from snake venom of Agkistrodon acutus
The haemorrhagin AaH Ⅲ isolated from the snake venom of Agkistrodon acutus is one of the few al-kaline ones in snake venoms. Its crystals belong to space group P212121 with a = 9. 573 4 nm, b = 4. 996 7 nm and c = 4. 728 8 nm. Its crystal structure was determined by the molecular replacement method according to the model of metalloproteinase Adamalysin n from eastern rattlesnake venom. The AaHⅢ structure has been refined by PROLSQ. The final R factor was 0.254 and the RMS deviations of bond lengths and angles were 0. 001 8 nm and 1.5°. The structure comparison suggested that AaHⅢ has a similar structure to other snake venom zinc-metalloproteinases. They all belong to matrix metalloproteinases super-family. 相似文献
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