Cloning of Salt Tolerance-Related cDNAs from the Mangrove Plant Sesuvium portulacastrum L.

In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant （Sesuvium portulacastrum L.）, a subtracted cDNA library was constructed via suppressive subtractive hybridization （SSH）, in which the poly（A）＋RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly（A）＋RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant （LEA） protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.     

Distribution of ground-dwelling beetles （Coleoptera） across a forest-clearcut ecotone in Wolong Natural Reserve, southwestern China

Abstract The influence of edge effect on ground-dwelling beetles (Coleoptera) across a forest-clearcut ecotone was studied in Wolong Natural Reserve, southwestern China. During the field research, a total of 30 739 beetles were collected with pitfall traps along transects, which extending 100 m from the edge into the forest interior and 100 m into the clearcut. Of the collection, Carabidae comprised 92%, Staphylinidae 3%, Curculionidae 2%, and Tenebrionidae 2%, and these four families can be considered as abundant groups. Family richness, Shannon diversity and equitability display a significant decrease from forest interior, edge to clearcut. Based on the family composition and abundance, ground-dwelling beetles of the forest interior can be separated from those in the clearcut by Principal coordinate analysis ordination, and beetle assemblages in the forest edge were more similar to forest assemblages than to those found in the clearcut by cluster analysis. Seasonal dynamics of family richness showed a monotone peak in the middle season, with a highest value in the forest interior and a lowest value in the clearcut. Family abundance showed two peaks in the middle season, always with more individuals in the clearcut than in the forest interior or in the edge. Multiple linear regression analyses showed that the cover of shrub and litter were the two most important factors in determining family richness, Shannon diversity, equitability and abundance. Our results show that the forest edge and clearcut have obviously different composition and diversity of ground-dwelling beetles from forest interior at the family level. However, more edges have been formed due to increasing forest fragmentation (clearcutting or logging), so it is necessary to preserve large and intact forest to protect the diversity of ground-dwelling beetles in Wolong Natural Reserve.     

医学图书馆馆藏建设的调查分析

探讨了目前医学图书馆馆藏结构的现状和馆藏建设策略的转变,在资金短缺的情况下,医学图书馆要确定有效的采购方案,保质保量,突出重点,加强网络协作,实现资源共享,以满足临床和科研的需要。     

检测猪繁殖与呼吸综合征病毒RT-PCR的建立及应用

根据GenBank上PRRSV的保守序列,设计1对引物,建立了PRRSV的RT-PCR方法。试验结果表明,该RT-PCR方法敏感、特异,适于临床样品检测,为PRRSV的临床诊断和流行病学调查奠定了基础。     

长江中游地区野生百合资源调查及利用前景

通过对长江中游地区野生百合资源进行调查,发现长江中游地区有野生百合资源14个种和3个变种即野百合(Lilium brownii F. E. Brown ex Miellez var. brownii)、百合(L. brownii F. E. Brown ex Miellez var. viridulum Baker)、宜昌百合(L. leucanthum (Baker) Baker)、渥丹(L. concolor Salisb. var. concolor)、有斑百合(L. concolor Sali sb. var. pulchellum (Fisch.)Regel)、滇百合(L. baberianum Coll.et Hemsl)、大理百合(L.taliense Franch.)、药百合(L.speciosum Thunb. var. gloriosoides Baker)、湖北百合(L.henryi Baker)、南川百合(L. rosthornii Diels)、宝兴百合(L. duchartrei Franch.)、山丹(L. pumilum DC.)、川百合(L. davidii Duchartre)、条叶百合(L. callosum Sieb. et Zucc.)、乳头百合(L. papilliferum Franch.)、绿花百合(L. fargesii Franch.)和卷丹(L. tigrinum Ker Gawler)。对野生百合在长江中游地区分布现状、观赏特性及开发利用价值进行了分析,为百合新品种培育奠定基础。同时对长江中游地区野生百合资源的保护和合理开发利用提出建议。     

过度采集的恶果

<正>在长白山保护区进行了三十多年野生动物调查研究的高级工程师朴正吉,自红松种子承包经营伊始,便克服了经费与人手不足的困难,坚持进行"过度采摘红松种子对野生动物影响"这个课题的独立研究。笔者先后组织北京林业大学徐化成教授,南京林业大学徐凤翔教授,中国科学院动物所汪松、沈孝宙、王瑛研     

金针虫调查方法及评价

金针虫是一类重要的地下害虫,其种群数量调查是植物保护工作中的难点之一。文章概述金针虫种群数量调查的3种主要方法:土方抽样、诱饵诱捕及成虫性信息素诱捕。就其调查原理、特点及研究现状进行了介绍,并对各方法的不足及应用前景做了简要分析。     

北京地区蝗虫种类研究及其垂直分布调查

经过2003~2004年对北京市平原区和山区的调查研究,初步发现北京市蝗虫有49种,隶属8科33属。由于植被、生态因素与环境条件和海拔高度的不同,蝗虫种类的垂直分布存在较明显的差异。对农林业为害较重的主要优势种有10种。     

广东省药用野生稻的调查收集与保护

为了全面了解广东药用野生稻自然生存现状,为我国野生稻资源的保护提供决策依据,2006、2012-2018年对广东药用野生稻生存状况进行了调查.结果查明,截至2018年12月,广东8个县市17个乡镇尚存药用野生稻,共有分布点21个,其中3个点为首次发现.现有分布点中,47.6％的分布点分布范围小于67 m2或只有零星几丛...     

Sampling techniques for estimating the population density of Apolygus lucorum in cotton fields

本文比较研究了棉田绿盲蝽Apolygus lucorum(Meyer-Dür)种群的调查方法.全株调查法中,目测法调查到的绿盲蝽种群密度最高,显著高于扫网法和盆拍法.局部调查法发现,蕾上的绿盲蝽种群数量显著高于叶片和花上;蕾上种群数量与整株密度之间呈显著正相关关系(y=1.18x+3.69).间接调查法表明:在苗期和蕾期,叶片受害等级和绿盲蝽种群密度之间呈显著正相关关系(苗期:y=25.28x+8.20;蕾期:y=43.99x+27.58).