一期后路半椎体切除短节段内固定与一期前路半椎体切除短节段内固定治疗先天性半椎体畸形的疗效对比

目的：对比一期后路半椎体切除短节段植骨融合内固定术与一期前路半椎体切除短节段植骨融合内固定术治疗先天性半椎体畸形的疗效。方法：抽取兰州军区兰州总医院骨科中心脊柱外科46例住院手术治疗先天性半椎体畸形的患者,随机化分为2组,每组23例,分别行一期后路半椎体切除短节段植骨融合内固定术和一期前路半椎体切除短节段植骨融合内固定术,观察比较两纽的手术时间、出血量、术后住院时间、术前和术后6个月侧凸cobb角、后凸cobb角及矫正率。结果：两组间的手术时间、出血量、术后住院时间、术后6个月后凸cobb角及后凸矫正率对比差异有统计学意义。结论：一期后路半椎体切除短节段植骨融合内固定术在后凸畸形矫正方面优于一期前路半椎体切除短节段植骨融合内固定术,且其手术创伤较小、术后恢复较快。     

手术室术前访视应用ipad对访视效果影响的研究

目的：探讨术前访视应用ipad在术前访视中对手术患者心理状态的影响。方法：将300例择期手术患者随机分为对照组（150例）和实验组（150例）,对照组采用常规术前访视方案;实验组在此基础上结合ipad展示围术期相关信息,采用焦虑自量表评估两组病人入院时和入手术室时的焦虑状况及血压、心率等指标,并且进行术后并发症和满意度的调查。结果：改良术前访视后焦虑评分、收缩压、舒张压、心率实验组明显低于对照组。结论：术前访视应用ipad可明显减轻手术病人术前的焦虑。     

大鼠肝纤维化病理分期与肝纤维化血清标志物相关性研究

目的：研究肝纤维化病理组织学分期与血清纤维化标志物门冬氨酸氨基转移酶（AST）与血小板（PLT）比值指数（APRI）、透明质酸（HA）及基质金属蛋白酶抑制物（TIMP-1）的相关性,以探讨非创伤性检测血清蛋白和联合分析血清纤维化标志物的诊断价值。方法：将75只成年健康的SD大鼠采用改良式复合因素法构建大鼠肝纤维化模型,分别在5、6个周后,进行大鼠肝脏穿刺活检病理学组织检查,HE、Masson染色后进行病理组织学分期,同时检测大鼠血清门冬氨酸氨基转移酶（AST）与血小板（PLT）比值指数（APRI）、透明质酸（HA）及基质金属蛋白酶抑制物（TIMP-1）水平。结果：肝脏组织病理学检查发现肝纤维化的分期与炎症活动程度呈明显的正相关（P〈0.05）。而对于区分显著肝纤维化（≥S2）血清标志物指标诊断纤维化的ROC曲线分析发现,HA、TIMP-1、APRI曲线下面积分别为0.921、0.732、0.905。HA＋APRI联合诊断灵敏度为84.1%,特异度为91.2%。TIMP-1＋APRI联合诊断灵敏度为83.2%,特异度为91.7%。结论：利用检测血清纤维化标志物这种对患者无创的检测方法虽不能完全取代病理学活检,但预测肝纤维具有一定的诊断价值,当联合分析两种血清纤维化标志物时,灵敏度和特异度都比单独分析一种标志物要高。     

原发性肝细胞癌组织中Beclin 1的表达及其意义

目的：探讨原发性肝细胞癌（HCC）组织中Beclin 1的表达及其在肝细胞癌发生发展中的作用。方法：应用免疫组化EnVision法检测54例肝细胞癌组织及其癌旁组织中Beclin 1的表达,分析其与肝细胞癌的病理分级、临床分期、侵袭及转移等临床病理因素的关系。结果：HCC组织中Beclin 1阳性表达率明显低于癌旁组织（X2=7.53,P〈0.01）。病理分级Ⅲ～Ⅳ、TNM分期Ⅲ～Ⅳ、存在静脉浸润和淋巴结转移的肝癌组织中的Beclin 1表达水平明显低于病理分级Ⅰ～Ⅱ、TNM分期Ⅰ～Ⅱ、无静脉浸润和淋巴结转移的肝癌组织（P〈0.01）。结论：HCC组织中存在Beclin 1表达缺失,可能与HCC的发生有着密切的关系,且对HCC的恶性生物学行为有重要影响。     

Soil microbial properties under different vegetation types on Mountain Han

This study investigated the influence of broadleaf and conifer vegetation on soil microbial communities in a distinct vertical distribution belt in Northeast China.Soil samples were taken at 0-5,5-10 and 10-20 cm depths from four vegetation types at different altitudes,which were characterized by poplar(Populus davidiana)(1250-1300 m),poplar(P.davidiana) mixed with birch(Betula platyphylla)(1370-1550 m),birch(B.platyphylla)(1550-1720 m),and larch(Larix principis-rupprechtii)(1840-1890 m).Microbial biomass and community structure were determined using the fumigation-extraction method and phospholipid fatty acid(PLFA) analysis,and soil fungal community level physiological profiles(CLPP) were characterized using Biolog FF Microplates.It was found that soil properties,especially soil organic carbon and water content,contributed significantly to the variations in soil microbes.With increasing soil depth,the soil microbial biomass,fungal biomass,and fungal catabolic ability diminished;however,the ratio of fungi to bacteria increased.The fungal ratio was higher under larch forests compared to that under poplar,birch,and their mixed forests,although the soil microbial biomass was lower.The direct contribution of vegetation types to the soil microbial community variation was 12%.If the indirect contribution through soil organic carbon was included,variations in the vegetation type had substantial influences on soil microbial composition and diversity.     

Facile fabrication of eco-friendly nano-mosquitocides: Biophysical characterization and effectiveness on neglected tropical mosquito vectors

Mosquito (Diptera: Culicidae) vectors are solely responsible for transmitting important diseases such as malaria, dengue, chikungunya, Japanese encephalitis, lymphatic filariasis and Zika virus. Eco-friendly control tools of Culicidae vectors are a priority. In this study, we proposed a facile fabrication process of poly-disperse and stable silver nanoparticles (Ag NPs) using a cheap leaf extract of Ichnocarpus frutescens (Apocyanaceae). Bio-reduced Ag NPs were characterized by UV–vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The acute toxicity of I. frutescens leaf extract and green-synthesized Ag NPs was evaluated against larvae of the malaria vector Anopheles subpictus, the dengue vector Aedes albopictus and the Japanese encephalitis vector Culex tritaeniorhynchus. Compared to the leaf aqueous extract, Ag NPs showed higher toxicity against A. subpictus, A. albopictus, and C. tritaeniorhynchus with LC₅₀ values of 14.22, 15.84 and 17.26 μg/mL, respectively. Ag NPs were found safer to non-target mosquito predators Anisops bouvieri, Diplonychus indicus and Gambusia affinis, with LC₅₀ values ranging from 636.61 to 2098.61 μg/mL. Overall, this research firstly shed light on the mosquitocidal potential of I. frutescens, a potential bio-resource for rapid, cheap and effective synthesis of poly-disperse and highly stable silver nanocrystals.     

自拟柴芍护膜汤联合莫沙必利治疗反流性食管炎的临床效果观察

目的:研究自拟柴芍护膜汤联合莫沙必利治疗反流性食管炎的临床效果和可能机制。方法:选择2015年12月～2018年12月我院内科杂病门诊治疗的70例反流性食管炎患者,将其随机分为两组。对照组服用莫沙必利,每次5 mg,每天3次;观察组联合服用自拟柴芍护膜汤以及莫沙必利。比较两组患者治疗后的临床治疗效果,治疗前后泛酸、烧心、脘腹胀满、嗳气、呃逆、情志抑郁、善太息、食欲不振以及嘈杂等症状的严重程度、血浆胃动素和血清胃泌素水平的变化。结果:治疗后,观察组的有效率为91.43%,明显高于对照组(71.43%,P0.05)。两组治疗后的泛酸、烧心、脘腹胀满、嗳气、呃逆、情志抑郁、善太息、食欲不振以及嘈杂评分均较治疗前明显降低(P0.05),且观察组的上述症状评分明显低于对照组(P0.05);两组治疗后的血浆胃动素和血清胃泌素水平均较治疗前明显升高(P0.05),且观察组以上指标均明显高于对照组(P0.05)。结论:自拟柴芍护膜汤联合莫沙必利能有效改善反流性食管炎的临床症状,其作用机制可能与提高血浆胃动素和血清胃泌素水平有关。     

血府逐瘀汤联合四联疗法治疗Hp阳性慢性萎缩性胃炎的临床观察

目的:探究血府逐瘀汤联合四联疗法治疗幽门螺旋杆菌(Hp)阳性慢性萎缩性胃炎的效果。方法:回顾性分析2014年5月-2017年12月在我院进行诊治的80例Hp阳性慢性萎缩性胃炎患者的临床资料,按照其入院顺序经随机数字表分为研究组和对照组,每组各40例患者。其中,对照组患者采用四联疗法,研究组患者在对照组基础上联合血府逐瘀汤进行治疗,对比两组患者的Hp转阴率、治疗前后胃黏膜病理积分的变化情况、临床症状积分的变化情况和不良反应发生率。结果:治疗后,研究组患者的Hp转阴率[85.0%(34/40)]显著高于对照组[62.5%(25/40)](P0.05)。两组上腹痛、纳差、上腹胀、反酸、嗳气等临床症状积分以及胃黏膜萎缩、肠化、不典型增生等病理积分均显著低于治疗前(P0.05),且研究组以上指标均明显低于对照组(P0.05)。研究组患者的不良反应发生率[5.0%(2/40)]与对照组[12.5%(5/40)]无显著性差异(P0.05)。结论:血府逐瘀汤联合四联疗法治疗Hp阳性慢性萎缩性胃炎的效果显著优于单用四联疗法,其可有效改善患者的病变程度和临床症状,且无明显不良反应产生。     

孕烷X受体配体结合结构域蛋白晶体的X线衍射结构解析

目的:利用X线衍射技术解析孕烷X受体(PXR)配体结合结构域(LBD)蛋白晶体的3维结构。方法:对PXR蛋白LBD(130~434氨基酸残基)序列进行密码子优化并化学合成后克隆至pRSFDuet-1表达载体,再将载体导入大肠杆菌BL21(DE3),对PXR-LBD蛋白进行原核表达与分离纯化;采用晶体筛选试剂盒筛选蛋白结晶条件,采用悬滴法获得目标蛋白的晶体;对获得的蛋白晶体进行X线晶体衍射检测,并收集相关数据建立PXR-LBD的三维结构。结果:获得了PXR-LBD的高质量晶体并利用X线衍射解析了该蛋白质晶体的结构数据,使用Phenix.refine软件和COOT软件等对结构进行修正,最终获得了高分辨率的3维结构数据。结论:完成了孕烷X受体配体结合结构域蛋白晶体的X线衍射结构解析,为研究和开发PXR相关药物奠定了基础。     

Expanded Expression Landscape and Prioritization of Circular RNAs in Mammals

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