MORPHOLOGICAL DESCRIPTION OF THE LARVAE OF CERATOPHYLLUS FARRENICHAOI AND THEIR COMPARISON WITH THE LARVAE OF CERATOPHYLLUS CALLINAE TRIBULIS

Abstract This paper deals with the morphology of the larvae of Cerutophyllus farreni chaoi Smit and Allen, 1955 and a comparison is made with the larvae of C. gallinue tribulis Jordan, 1926.
The diagnostic characters of the larvae of the two subspecies are the shape of egg burster, number of mandibular teeth, the relative positions of the sense organs and setae on the tergites of the thorax and abdomen and number of the setae of anal comb and the strut setae.     

Synthesis and antibiofilm evaluation of 3-hydroxy-2,3-dihydroquinazolin-4(1H)-one derivatives against opportunistic pathogen Acinetobacter baumannii

The emergence of multidrug resistant microorganisms has triggered the impending need for new aitimicrobial strategies. The antivirulence strategy with the benefite of alleviating the drug resistance becomes the focus of research. In this study, 22 quorum sensing inhibitors were synthesized by mimicking the structure of autoinducer and acinetobactin and up to 34% biofilm inhibition was observed with 5u. The biofilm inhibition effect was further demonstrated with extracellular polysaccharides inhibition and synergism with Gentamycin sulphate.     

Bisbenzylisoquinoline alkaloids and P-glycoprotein function: A structure activity relationship study

Conflicts with the notion that specific substrate interactions were required in the control of reaction path in active transport systems, P-glycoprotein showed extraordinarily low specificity. Therefore, overexpression P-glycoprotein excluded a large number of anticancer agents from cancer cells, and multidrug resistance happened. Several kinds of bisbenzylisoqunoline alkaloids were reported to modulate P-glycoprotein function and reverse drug resistance. In order to provide more information for their structure activity relationship on P-glycoprotein function, the effects of tetrandrine, isotetrandrine, fangchinoline, berbamine, dauricine, cepharanthine and armepavine on the P-glycoprotein function were compared by using daunorubicin-resistant leukemia MOLT-4 cells in the present study. Among them, tetrandrine exhibited the strongest P-glycoprotein inhibitory effect, followed with fangchinoline and cepharanthine, and subsequently with berbamine or isotetrandrine. However, dauricine and armepavine showed little influence on the P-glycoprotein function. These data revealed that the 18-membered ring of the bisbenzylisoquinoline alkaloids maintained the P-glycoprotein inhibitory activity, suggesting that double isoquinoline units connected by two oxygen bridges were indispensable. Moreover, stereo-configuration of bisbenzylisoquinoline 3D structures determined their inhibitory activities, which provided a new viewpoint to recognize the specificity of binding pocket in P-glycoprotein. Our data also indicated that 3D chemical structure was more sensitive than 2D to predict the P-glycoprotein inhibitory-potencies of bisbenzylisoqunoline alkaloids.     

温度诱导Targetron系统用于大肠杆菌高效基因失活

构建基于TeI3c/4c嗜热二型内含子的温度诱导Targetron基因失活系统 (Thermotargetron),并应用于中温微生物基因编辑。在大肠杆菌HMS174 (DE3) 基因组中,选择Subunit of flagellum基因 (fliC) 和C4 dicarboxylate orotate:H+ symporter基因 (dctA) 为靶基因。根据TeI3c/4c DNA识别规则,在fliC和dctA基因中选择fliC489a、fliC828s、fliC1038s和dctA2a位点为基因打靶位点。使用重叠延伸PCR方法,基于pHK-TT1A质粒构建打靶载体。打靶载体转化HMS174菌株,对数期转化子培养液48 ℃热激1 h后涂布于氯霉素抗性LB平板上。使用菌落PCR和DNA测序检测突变株并计算基因失活效率。获得突变株后,通过琼脂穿刺和碳源代谢实验,鉴定ΔfliC、ΔdctA突变株表型变化。菌落PCR测序结果表明,TeI3c/4c插入到fliC和dctA基因设计位点,且打靶效率高达100%。突变株表型验证实验表明,ΔfliC突变株运动能力显著下降,ΔdctA突变株苹果酸代谢能力缺失。综上所述,文中建立了一套适用于嗜中温微生物的温度诱导型、高效基因失活系统,该系统可通过控制宿主菌在48 ℃保温时间实现高效、靶向、精准基因失活。     

不同温度对迷人杜鹃种子萌发与幼苗生长及生理特性的影响

该研究利用实验室不同恒温和变温控制试验,考察了不同温度[恒温(15℃、25℃、35℃)和变温(25℃/15℃、30℃/20℃,高温12 h,低温12 h)]处理对迷人杜鹃种子萌发、幼苗形态指标和生理生化指标的影响,探讨温度对迷人杜鹃种子萌发和幼苗生长的影响机制。结果表明:(1)迷人杜鹃种子在25℃/15℃变温条件下萌发率(87.69%)和萌发指数(8.65)均最高。(2)25℃/15℃变温有利于迷人杜鹃幼苗的地径、苗高、平均根长和萌枝数的增加,以及根、茎、叶生物量的积累。(3)25℃/15℃变温处理下幼苗叶片的总叶绿素、可溶性糖和可溶性蛋白含量最高,而MDA含量、CAT活性和SOD活性较低。研究认为,迷人杜鹃种子萌发和幼苗生长的最适宜温度条件为25℃/15℃(昼/夜),而在高温(35℃)和低温(15℃)环境下均会受到显著抑制。     

解纤维梭菌脱水四环素诱导的ClosTron基因打靶系统构建

【背景】解纤维梭菌是发酵木质纤维素生产乙醇的中温模式菌株,构建可控诱导的基因打靶技术是研究解纤维梭菌遗传调控的重要手段。【目的】在解纤维梭菌中构建脱水四环素诱导的ClosTron基因打靶系统。【方法】首先,分析解纤维梭菌对脱水四环素的敏感性,筛选合适的诱导剂浓度,并以β-葡萄糖苷酶为报告基因,检测其在解纤维梭菌中的诱导效果。然后,将脱水四环素诱导型操纵子与II型内含子元件组合,构建脱水四环素诱导的ClosTron质粒。最后,以解纤维梭菌mspI、ldh和ack为例,检测其在解纤维梭菌中的打靶效率。【结果】脱水四环素诱导系统在解纤维梭菌中能够诱导ClosTron元件表达,在mspI、ldh和ack这3个基因位点的打靶效率分别为29.48%±15.51%、23.61%±7.08%和28.09%±6.97%。【结论】在解纤维梭菌中成功构建脱水四环素诱导的ClosTron基因打靶系统,为梭菌基因工程改造奠定了基础。     

过表达胃泌素促进胃癌细胞的增殖、迁移和侵袭

胃癌患者转移淋巴结中胃泌素基因的表达量是原发胃癌组织的42倍,推测胃泌素可能与胃癌转移密切相关. 本文通过构建含胃泌素基因的真核表达载体,成功获得过表达胃泌素的稳转胃癌细胞株AGS和SGC-7901, 并用MTT、细胞伤愈实验、Transwell 小室实验及ELISA检测过表达胃泌素对细胞迁移、侵袭及转移相关蛋白基质金属蛋白酶2（MMP-2）分泌能力的影响. 结果显示,过表达胃泌素稳转细胞的相对增殖率、 迁移入细胞致伤区的相对距离比对照组高,迁移和侵袭到Transwell下室面的细胞, 以及培养液中每mg蛋白质的MMP-2浓度也高于对照组的细胞. 结果提示,胃泌素通过促进胃癌细胞分泌MMP-2来增强细胞的迁移和侵袭能力. 该研究对揭示胃癌转移的分子机制具有重要意义.     

Long Noncoding RNA SNHG5 Knockdown Alleviates Neuropathic Pain by Targeting the miR-154-5p/CXCL13 Axis

Neurochemical Research - Neuropathic pain is an unneglectable pain condition with limited treatment options owing to its enigmatic underlying mechanisms. Long noncoding RNA small nucleolar RNA host...     

家蝇溶菌酶MDLZM2基因的克隆、序列分析及原核表达

对家蝇溶菌酶(Musca domestica lysozyme,MDLZM2)基因进行克隆、序列分析,构建原核表达载体并在大肠杆菌中表达。从Gen Bank家蝇基因组中筛选获得MDLZM2基因。以该基因的序列设计引物,进行PCR扩增,测序分析获得该基因完整编码序列。运用生物信息学方法对该基因及其编码蛋白的基本理化性质、信号肽、二级结构、三级结构和保守结构域等方面进行预测和分析。构建p EASY-E1-MDLZM2重组质粒,转化到大肠杆菌BL21(DE3)p Lys S Chemically Competent Cell中进行诱导表达及纯化。结果表明MDLZM2基因ORF全长552 bp,编码183个氨基酸,理论分子量21.2 k Da;等电点为6.13,具有Lysozyme家族的蛋白保守结构域。成功构建重组原核表达p EASY-E1-MDLZM2并诱导表达、纯化重组蛋白,为进一步研究该蛋白的生物学及免疫学活性奠定了基础。     

妊娠哺乳期乳腺癌患者的预后情况及其影响因素分析

目的:分析妊娠哺乳期乳腺癌患者的预后情况及其影响因素。方法:将2010年1月至2012年12月期间贵州省贵阳市妇幼保健院、贵州省医科大学和贵州省肿瘤医院收治的妊娠哺乳期乳腺癌患者60例作为研究组,选择同期收治的非妊娠哺乳期乳腺癌患者60例作为对照组,比较两组5年生存率和临床病理特征,并应用单因素和多因素Logistic回归分析患者预后的影响因素。结果:研究组5年生存率为61.67%(37/60),5年无病生存率为46.67%(28/60),均低于对照组的81.67%(49/60),73.33%(44/60)(P0.05)。研究组肿瘤最大直径、腋淋巴结转移率、TNM分期为III期的比例、雌激素受体阳性率、Ki67细胞阳性率≥20%的比例均高于对照组(P0.05),两组孕激素受体阳性率比较无统计学差异(P0.05)。单因素分析显示妊娠哺乳期乳腺癌患者总生存期与肿瘤最大直径、TNM分期、Ki67细胞阳性率≥20%的比例、腋淋巴结转移率有关(P0.05)。多因素Logistic回归分析显示,TNM分期为III期、Ki67细胞阳性率≥20%、腋淋巴结转移是影响妊娠哺乳期乳腺癌患者预后的独立危险因素(P0.05)。结论:妊娠哺乳期乳腺癌患者的预后较差,TNM分期为III期、Ki67细胞阳性率≥20%、腋淋巴结转移是影响患者预后的危险因素,对于临床防治具有重要的启示作用。