The increasing world population has forced excessive chemical fertilizer and irrigation to complete the global food demand, deteriorating the water quality and nutrient losses. Short-term studies do not compile the evidences; therefore, the study aimed to identify the effectiveness of reduced doses of inorganic fertilizer and water-saving practices, hence, a six-year experiment (2015–2020) was conducted in China to address the knowledge gap. The experimental treatments were: farmer accustomed fertilization used as control (525:180:30 kg NPK ha−1), fertilizer decrement (450:150:15 kg NPK ha−1), fertilizer decrement + water-saving irrigation (450:150:15 kg NPK ha−1), application of organic and inorganic fertilizer + water-saving irrigation (375:120:0 kg NPK ha−1 + 4.5 tones organic fertilizer ha−1), and application of controlled-release fertilizer (80:120:15 kg NPK ha−1). Each treatment was replicated thrice following a randomized complete block design. The results achieved herein showed that control has the highest losses in the six-year study for total nitrogen (225.97 mg L−1), total soluble nitrogen (121.58 mg L−1), nitrate nitrogen (0.93 mg L−1), total phosphorus (0.57 mg L−1), and total soluble phosphorus (0.57 mg L−1) respectively. Reduced fertilizer and water application improved crop nutrient uptake, nitrogen concentration was significantly enhanced with organic and inorganic fertilizer + water-saving irrigation, P concentration was increased with fertilizer decrement + water-saving irrigation, and K concentration was improved with fertilizer decrement + water-saving irrigation. Hence, this study concludes that reduced inorganic fertilizer dose combined with water-saving practices is significantly helpful in reducing nutrient leaching losses and improving nutrient uptake and water pollution. Further studies are needed to explore the impacts of reduced fertilization and water-saving irrigation on leaching losses. The benefits at different climatic conditions, soil types, and fertilizer types with application methods are also a research gap.
Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) is an adaptor protein of the pattern recognition receptors NOD1 and NOD2 involved in regulating inflammatory response and resisting pathogenic microbial infection. In this study, Acipenser baeriiRIPK2 (AbRIPK2) was identified. The open reading frame of AbRIPK2 was 1815 bp encoding 604 amino acids. AbRIPK2 possessed the typical N-terminal kinase domain (KD) and C-terminal caspase recruitment domain (CARD). The phylogenetic tree analysis revealed that AbRIPK2 shared a relatively high identity with bony fish. Real-time fluorescence quantitative PCR (qRT-PCR) results indicated that AbRIPK2 was highly expressed in the gill, followed by muscle, liver and heart. AbRIPK2 was significantly induced in the spleen and valvular intestine after Streptococcus iniae and Aeromonas hydrophila infection. AbRIPK2 was significantly upregulated after peptidoglycan (PGN) treatment in the splenic leukocytes. This study indicated that AbRIPK2 played a potential role in resisting the pathogenic infection of Siberian sturgeon by responding to bacteria. 相似文献
Myxococcus xanthus cells aggregate and develop into multicellular fruiting bodies in response to starvation. A new M. xanthus locus, designated dif for defective in fruiting, was identified by the characterization of a mutant defective in fruiting body formation. Molecular cloning, DNA sequencing and sequence analysis indicate that the dif locus encodes a new set of chemotaxis homologues of the bacterial chemotaxis proteins MCPs (methyl-accepting chemotaxis proteins), CheW, CheY and CheA. The dif genes are distinct genetically and functionally from the previously identified M. xanthus frz chemotaxis genes, suggesting that multiple chemotaxis-like systems are required for the developmental process of M. xanthus fruiting body formation. Genetic analysis and phenotypical characterization indicate that the M. xanthus dif locus is required for social (S) motility. This is the first report of a M. xanthus chemotaxis-like signal transduction pathway that could regulate or co-ordinate the movement of M. xanthus cells to bring about S motility. 相似文献
IRBP is a photoreceptor-specific glycoprotein that has been suggested as a retinoid carrier in the visual process. Previous research has shown that 1.3 kb of 5'-flanking sequence from the human IRBP gene is sufficient to promote photoreceptor-specific expression of reporter genes in transgenic mice. To define more narrowly the sequences that promote tissue-specific expression, chimeric constructs with shorter promoters were used to generate transgenic mice. The bacterial CAT gene was fused to fragments of 706 bp or 212 bp from the 5' end of the human IRBP gene. Analysis of the three transgenic families bearing the 706 bp IRBP promoter revealed that CAT expression was confined to the neuro-retina and the pineal gland. Analysis of the four transgenic families bearing the 212 bp IRBP promoter revealed the same tissue-specific CAT expression in three families. These results establish that tissue-specific expression of IRBP can be regulated by a short 212 bp promoter which has been conserved between humans and mice. 相似文献
Abstract The susceptibility and virulence of entomopathogenic fungus Metarhizium anisopliae var. acridum to the stages of brown planthopper (BPH), Nilaparvata lugens (Stål) and whitebacked planthopper (WBPH), Sogatella furcifera (Horvath) were investigated under laboratory conditions. Three dosages of M. anisopliae var. acridum ranging from 10.5, 116.3 and 1027.1 conidial/mm2 were used in the experiment. The tested stages of host included three developmental stages, young nymphs (1–2 instars), old nymphs (3–5 instars) and adults. It was found that all tested stages of the planthoppers were susceptible to the fungal infection. The degree of virulence LT50 of M. anisopliae var. acridum against young nymphs of N. lugens are >21, 20.82 and 16.55, respectively with the 3 dosages, the LT50 of the fungus against the old nymphs are 17.68, 15.49 and 13.98, respectively with the 3 dosages; the LT50 of the fungus against the adults are 17.10, 12.57 and 9.14 respectively with the 3 dosages. The degree of virulence LT50 of M. anisopliae var. acridum on young nymphs of S. furcifera are >21, 17.29 and 13.13, respectively with the 3 dosages ; the LT50 of the fungus against the old nymphs are 16.94, 15.02 and 13.03, respectively with the 3 dosages; the LT50 of the fungus against the adults are 12.78, 10.16 and 7.64, respectively with the 3 dosages. Adults were more susceptible to M. anisopliae var. acridum infection than their nymphs and the young nymphs were most resistant to the fungal infection. The cumulative mortality of each stage was dosage-dependent. Of all the developmental stages, WBPH was more susceptible than BPH to M. anisopliae var. acridum infection with the same dosages. 相似文献
Myxococcus xanthus is a gram-negative soil bacterium which exhibits a complex life cycle and social behavior. In this study, two developmental mutants of M. xanthus were isolated through Tn5 transposon mutagenesis. The mutants were found to be defective in cellular aggregation as well as in sporulation. Further phenotypic characterization indicated that the mutants were defective in social motility but normal in directed cell movements. Both mutations were cloned by a transposon-tagging method. Sequence analysis indicated that both insertions occurred in the same gene, which encodes a homolog of DnaK. Unlike the dnaK genes in other bacteria, this M. xanthus homolog appears not to be regulated by temperature or heat shock and is constitutively expressed during vegetative growth and under starvation. The defects of the mutants indicate that this DnaK homolog is important for the social motility and development of M. xanthus.相似文献