中国木薯栽培种通过器官发生再生植株研究

本文研究了中国木薯栽培种四种外植体通过器官发生再生植株的条件。结果表明:在MS附加0.05mg/L TIBA,1mg/L BA的培养基上“NZ 188”初步的萌发胚状体“切头”后切口处可直接产生丛芽,出芽率为43%。“SC201”胚状体子叶块在MS附加0.5 mg/L NAA,0.5mg/L BA的培养基上可直接出芽,出芽率为42%,在MS附加0.5mg/L IBA,1.5mg/L BA培养基上·出芽率为31%,AgNO_3和ABA单独使用或配合使用均不利于芽的再生。“NZ188”胚状体下胚轴在MS附加0.5mg/LNAA,0.5mg/L BA的培养基上形成的愈伤组织转入MS附加1mg/L NAA,2mg/L BA的培养基上,3周后大多数愈伤组织有绿点出现、仅4.4%外植体分化出芽。“HZ188”无菌苗茎段接种在MS附加0.05mg/L TIBA,2mg/LBA的固体培养基上,2周后形成大量愈伤组织,4周后仅见一块愈伤组织分化出芽。

IN VITRO PLANT REGENERATION FROM CHINESE CASSAVA CULTIVARS VIA ORGANGENESIS

Chinese cassava cultivars in vitro regeneration from 4 types of explants was studied. The results showed that thick shoots developed directly from the cut surfaces of "NZ 188" decapitated germination embryos on MS medium supplemented with 0. 05mg/L TIBA, 1.0mg/L BA with a regeneration frequency of 43%. When inoculated on MS medium with 0. 5mg/L NAA, 0. 5mg/L BA for 3 weeks, cotyledons from "SC 201" mature embryos produced shoots directly at a frequency of 42% , on MS medium with 0. 5mg/ L IBA, 1. 5mg/L BA, at a freuqency of 31%. Adding AgNO3 only or both AgNO3 and ABA to the media had an unfavorable effect on the shoot-induction. Calli could be induced when hypocotyl segments from "HZ 188" germination embryos were cultured on MS medium supplemented with 0. 5mg/L NAA, 0. 5mg/L BA in two weeks. After 4 weeks of culture on MS medium with 1mg/ L NAA, 2 mg/L BA green dots appeared on most calli, only 4. 4% calli produced shoots. Calli formed two weeks later when "NZ 188" stem segments were cultured on MS medium with 0. 05mg/L TIBA, 2mg/L BA. After 4 weeks of subculture only one callus produced shoots on the same medium.