陆地棉的核型模式

陆地棉的核型公式为2n=4x=52=30m+20sm(4SAT)+2st(2SAT); 染色体绝对长度平均3.40μm; 臂比值最大与最小的分别是4.02和1.14,平均1.77;随体3对,分别位于第12、19和26对同源染色体的短臂上;最长与最短染色体的比值为2.36;其核型属于Stebbin的2B类型。 Abstract:Karyotype formula of Gossypium hirsutum L.was 2n=4s=52=30m+20m (4SAT)+2st (2SAT); and its absolute lengths of chromosomes averaged 3.40μm; arm ratio differed from 1.24 to 4.02,with the mean value of 1.77; three pairs of satellite chromosomes; the ratio of the longest to the shortest chromosomes was 2.36; and the karyotype belonged to 2B in Stebbin’s classification.     

角绥螨属在中国首次发现及一新种记述（蜱螨亚纲：囊螨科）

本文记述角绥螨属一新种,中华角绥螨Ａｎｔｅｎｎｏｓｅｉｕｓ ｓｉｎｉｃｕｓ ｓｐ．ｎｏｖ．,标本采自云南剑川县大绒鼠巢中。该属在中国尚系首次发现。     

中国若干森林水文要素地理分布规律的模拟

利用全国各森林植被地理分布区水文要素的实验观测数据,应用多元统计回归和主分量分析等数学方法建立了描述中国森林水文要素与地理要素（经度、纬度、海拔）之间函数关系Ｆ的三维地学模型（ＤＴＭ）,其中包括：全国年均降水分布规律模拟模型;全国林冠降水截留规律模拟模型;全国森林枯落物持水能力模拟模型;中国森林综合截留能力指数模型。并且利用地理信息系统的三维地学模型（ＤＴＭ）成图功能,实现全国范围内的森林水文要素     

琼脂糖电内渗对电泳的影响

用电内渗不同(0、0.03、0.08、0.20mr)的琼脂糖制成凝胶或电极缓冲液凝胶条,观察对电泳行为的影响.结果表明等电聚焦电泳必须使用无电内渗琼脂糖.不同电内渗的琼脂糖制成的电极缓冲液凝胶条对SDS电泳无显著影响,但对常规聚丙烯酰胺凝胶电泳有不同程度的影响,电内渗越高,越不利于电泳的进行.     

生物实验数据的某些非线性分析方法

简要介绍常用的非线性动力学参量,结合生物学实验数据的特点,给出几种最新的分析方法.     

转铁蛋白分型及其基因频率分布

用固相pH梯度(pH 5.05～5.60)等电聚焦技术对随机抽取的188名北京地区健康汉族人的血清转铁蛋白(Tf)进行分型调查,并统计基因频率,检出了在中国还未见报道的Tf^C3基因.其表型分别是：TfC1, TfC2, TfC1C2, TfC1C3, TfC1Dchi, TfC2Dchi.Tf^C1=0.7420, Tf^C2=0.2420, Tf^C3=0.0027, Tf^Dchi=0.0133, 符合Hardy-Weinberg定律, 并与其他已见报道的汉族 Tf基因频率大致相符.     

Expression of betaine aldehyde dehydrogenase gene and salinity tolerance in rice transgenic plants

Betaine as one of osmolytes plays an important role in osmoregulation of most high plants. Betaine aldehyde dehydrogenase C BADH) is the second enzyme involved in betaine biosynthesis. The BADH gene from a halophite, Atriplex hortensis, was transformed into rice cultivars by bombarment method. Totally 192 transgenic rice plants were obtained and most of them had higher salt tolerance than controls. Among transgenic plants transplanted in the saline pool containing 0.5% NaCl in a greenhouse, 22 survived, 13 of which set seeds, and the frequency of seed setting was very low, only 10% . But the controls could not grow under the same condition. The results of BADH ac-tivity assay and Northern blot showed that the BADH gene was integrated into chromosomes of transgenic plants and expressed.     

Sequence variation and genetic diversity in the giant panda

About 336–444 bp mitochondrial D-loop region and tRNA gene were sequenced for 40 individuals of the giant panda which were collected from Mabian, Meigu, Yuexi, Baoxing, Pingwu, Qingchuan, Nanping and Baishuijiang, respectively. 9 haplotypes were found in 21 founders. The results showed that the giant panda has low genetic variations, and that there is no notable genetic isolation among geographical populations. The ancestor of the living giant panda population perhaps appeared in the late Pleistocene, and unfortunately, might have suffered bottleneck attacks. Afterwards, its genetic diversity seemed to recover to some extent. Project supported by the “8.5” Key Project of Chinese Academy of Sciences, the Chairman Foundation of Chinese Academy of Sciences, K. C. Wang Education Foundation, the Applied Basic Research Foundation of Yunnan, the National Natural Science Foundation of China, the Special Foundation for Returned Chinese Scientists, and Zoological Society of San Diego.     

中缝核提取液对体外培养的中脑黑质神经元存活和生长的影响

中脑黑质是中缝核５－ＨＴ神经元最早期的靶组织之一。为了分析神经元对其靶细胞的作用是受某种特异性蛋白或神经营养因子的影响,本文用中缝核提取液作用于体外培养的中脑黑质神经元,证明了该提取液能促进中脑黑质神经元的存活和生长,其活性部分是分子量大于３０ｋＤ的组份,经ＳＤＳ－聚丙烯酰胺梯度凝胶电泳,呈现一条主带,分子量在４３ｋＤ左右。     

四种鱼类外周血红细胞细胞周期及DNA含量

通过流式细胞仪测量了兴国红鲤（Ｃｙｐｒｉｎｕｓ ｃａｒｐｉｏ Ｖａｒ． Ｓｉｎｇｕｏｎｅｎｓｉｓ）、奥利亚罗非鱼（Ｓａｒｏｔｈｅｒｏｄｏｎ ａｕｒｅａ）、 鳙（Ａｒｉｓｔｉｃｈｔｈｙｓ ｎｏｂｉｌｉｓ Ｒ．）和团头鲂（Ｍｅｇａｌｏｂｒａｍａ ａｍｂｌｙｃｅｐｈａｌａ Ｙｉｎ．）红细胞的ＤＮＡ含量,四种鱼与鸡红细胞ＤＮＡ含量的比值分别为１·６５、０．９６、０．９１、１·１５,以鸡红细胞ＤＮＡ含量２．３ｐｇ／Ｎ计算,四种鱼二倍体体细胞的 ＤＮＡ含量分别为３．８０ ｐｇ／Ｎ, ２． ２２ｐｇ／Ｎ、２． ０８ｐｇ／Ｎ、 ２． ６６ｐｇ／Ｎ。另外,四种鱼外周血红细胞分别有２６％、 ２６％、 ２３％、 ２４％的细胞处于 ＤＮＡ合成期（Ｓ）,ＤＮＡ合成后期（Ｇ２）和分裂期（Ｍ）,这表明这四种鱼类的外周血红细胞并非失去分裂能力的特化细胞群,它们表现了较强的细胞周期现象。这有可能是这几种硬骨鱼类外周血仍具有造血功能的缘故。