Expression of betaine aldehyde dehydrogenase gene and salinity tolerance in rice transgenic plants

Betaine as one of osmolytes plays an important role in osmoregulation of most high plants. Betaine aldehyde dehydrogenase C BADH) is the second enzyme involved in betaine biosynthesis. The BADH gene from a halophite, Atriplex hortensis, was transformed into rice cultivars by bombarment method. Totally 192 transgenic rice plants were obtained and most of them had higher salt tolerance than controls. Among transgenic plants transplanted in the saline pool containing 0.5% NaCl in a greenhouse, 22 survived, 13 of which set seeds, and the frequency of seed setting was very low, only 10% . But the controls could not grow under the same condition. The results of BADH ac-tivity assay and Northern blot showed that the BADH gene was integrated into chromosomes of transgenic plants and expressed.     

甜菜碱醛脱氢酶基因在水稻中的表达及转基因植株的耐盐性研究

甜菜碱醛脱氢酶(BADH)是渗透调节剂甜菜碱生物合成中涉及的第2个酶,我们将含盐生植物山菠菜BADH基因的植物双元表达载体经基因枪法导入水稻,经盐胁迫筛选得到转化植株,经RAPD检测全部阳性,在随机选择的10株转化植株中全部测出BADH活性,而对照未见.Northern杂交表明,其中7株为阳性.在含0.5%氯化钠的盐池中大多数转基因植株生长基本正常,结实率约为10%,而对照受盐害现象显著,几乎全部枯萎.