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971.
锌酵母中酵母甘露多糖组分的特征和结构 总被引:4,自引:0,他引:4
本文研究从锌酵母中分离出的酵母甘露多糖XP的特征和结构。XP经全水解和^13CNMR谱显示除甘露糖基外,还有少量L-鼠李糖基和甲氧基。甲基化分析、过碘酸盐氧化、Smith降解、乙酰解和部分酸水解显示XP的主链是1→6连接的甘露糖,侧链是1→2连接的甘露糖。^1H及^13C NMR谱表明所有糖苷键均为α型,结合元素分析XP基本是酵母甘露多糖和蛋白质以及锌的络合物。 相似文献
972.
同步辐射小角X射线散射(SAXS)实验站所用的探测器由原来的NaI闪烁计数器改为位置灵敏探测器,其位置分辨好于100 μm,有效探测面积为50 mm×10 mm,得到了较以往更高质量的SAXS谱,并成功地对液体样品的SAXS谱进行了测试. 相似文献
973.
INTR0DUCTI0NIn0urprevi0usstudy0fV5andVKfromfouranti-TCSIgEhybrid0masestab-lishedin0urlaboratory,wefoundVKfr0mallfourcl0nesusedfragmentsfromthesamegermlinegenefamilyVK21,andabiasintheuse0fJk1genefragmentwasalsoobserved.Ontheotherhand,thegeneusageofVHwaJsquitediverse.WespeculatedthatinIgEresponsest0TCS,thelightchainmayplayam0reimpor-tantroleinspecificbindingtoallergenicdeterminant0nTCS[1].However,duet0thelimitationofhybrid0matechnology,itisdifficulttoachievealargenumberofanti-TCSI… 相似文献
974.
Human gastric cancer MKN-45 cells were transfected with pULB 3238,a plasmid carrying MVMp MS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR.After the integration and expression of NS-1 gene,some of the transfectants died,while others remained alive,but the growth features of survived cells were changed.For further study on the antineoplastic function of parvoviral NS-1 protein in vivo,transgenic mice carrying NS-1 genes were established by conventional method.Among 4 founders,one of them was found to be able to transmit the transgene to around 50% of their offsprings.RT-PCR was performed to indicate the expression of NS-1 gene in transgenic mice and its mRNA appeared in a variety of tissues.The expression of integrated NS-1 gene may correlate with the decreased incidence of tumor induced in vivo by chemical carcinogens. 相似文献
975.
A cDNA coding mutated cecropin CMIV from Bombyx mori was synthesized according to its amino acid sequense using E .coli biased codons .The gene was cloned into the fusion expression vector pEZZ318 and was expressed in E .coli HB101.The fusion protein produced was purified by affinity chromatography to yield 26 mg/L fusion product .The anti-bacterial activities of recombinant cecropin CMIV were recovered after cleavage by chemical method. 相似文献
976.
Using genomic in-situ hybridization (GISH) technique, 7 translocation-addition lines, 6 transloca-tion and translocation-addition lines, 2 ditelosomic addition lines and 1 translocation line were identified from Triticum aestivum L.-Psathyrostachys juncea (Fisch. ) Nevski intergeneric hybrids, of which translocation-addition and translocation and translocation-addition lines were not found in other reports. No substitutions and disomic additions were detected in the hybrids and breakages occurred in all P. juncea chromosomes studied. Results have shown that the improved GISH technique is a rapid and economical method for use in this field. 相似文献
977.
A cDNA coding mutated cecropin CMIV fromBombyx mori was synthesized according to its amino acid sequence usingE. coli biased codons. The gene was cloned into the fusion expression vector pEZZ318 and was expressed inE. coli HB101. The fusion protein produced was purified by affinity chromatography to yield 26 mg/L fusion product. The anti-bacterial
activities of recombinant cecropin CMIV were recovered after cleavage by chemical method. 相似文献
978.
本研究用结扎盲肠及穿刺(CLP)引起败血症。结果证明:大鼠心肌钙通道在早期败血症(ES,CLP后9h)时由心肌轻型囊泡向心肌肌膜转运增多;在晚期败血症(LS,CLP后18h)时由心肌肌膜向心肌轻型囊泡转运增多。败血症时大鼠心肌肌膜和心肌轻型囊泡钙通道的再分布与cAMP依赖性蛋白激酶(PKA),Ca(2+)/钙调素依赖性蛋白激酶(PKM)和蛋白激酶C(PKC)磷酸化作用无关。败血症时大鼠心肌肌膜和心肌轻型囊泡上肾上腺能β-受体、M-胆碱受体和Na+/K+ATPase的变化规律和钙通道的一样,它们可能是败血症时的一种非特异性变化。 相似文献
979.
980.