Fusion expression of mutated cecropin CMIV in E. coli |
| |
Authors: | XIE Wei QIU Qifeng DONG XueyinHUA Zichun and XU Xianxiu I |
| |
Abstract: | A cDNA coding mutated cecropin CMIV from Bombyx mori was synthesized according to its amino acid sequense using E .coli biased codons .The gene was cloned into the fusion expression vector pEZZ318 and was expressed in E .coli HB101.The fusion protein produced was purified by affinity chromatography to yield 26 mg/L fusion product .The anti-bacterial activities of recombinant cecropin CMIV were recovered after cleavage by chemical method. |
| |
Keywords: | cecropin fusion expression E coli |
本文献已被 CNKI 等数据库收录! |