近交系BALB/c小鼠及先天遗传性白内障突变型BALB/cBk-Cat小鼠的DNA指纹研究

采用人工合成的寡核苷酸探针(GATA)~4|分别与近交系小鼠BALB/c和经10年 30代培育而成的自发突变型近交系先天遗传性白内障BALB/cBk-Cat小鼠进行DNA分杂交,DNA指纹显示:(1)BALB/c小鼠个体之间以及BALB/cBk-C at小鼠个体之间没有差异;(2)BALB/c小鼠与BALB/cBk-Cat小鼠的DNA指纹在23-6kb之间的区域有差异。     

香烟烟雾水溶物诱发蚕豆根尖细胞微核的研究

本文研究了不同浓度的香烟烟雾水溶物对蚕豆根尖细胞微核的诱变作用,结果表明, 上述水溶物能够引起蚕豆根尖细胞微核率发生敏感性变化,提示蚕豆根尖细胞微核技术可作为一种香烟遗传毒理学检垛方法使用。此外,还对使用该方法的意义以及添加VE对香烟烟雾水溶物微核率的影响进行了讨论。     

葡萄花药三倍体植株的倍性鉴定

用卡宝品红染色法检查格列那什二倍体葡萄(Vitis vinifera var. Cre nache)花药再生植株的染色体, 并用UNICHRO软件对其数目及长度进行了测量。结果表明,这些花药植株根尖中染色体组型为2n=3x=57的细胞平均占观察细胞总数的86.5%,来自不同花药的再生殖株均为三倍体。讨论了这些植株的可能来源。 Abstrart:The chromosome number in the root tip of triploid plants regenerated from anther culture of diploid Vitis vinifera var.Grenache were examined with Carbolfuchsin staining method and the length of chromosomes were measured with UNICHRO package.The results show that average pecentage of cells which have a karyotype for 2n=3x=57 in root tip of pollen plants were 86.5% in the total number of cells observed,the plants regenerated from different anthers are triploid.Their possible origin is discussed.     

不同添加物质对香烟烟雾水溶物引起的蚕豆根尖细胞微核率的影响

本课题研究了不同添加物质对香烟烟雾水溶物引起的蚕豆根尖细胞微核率的影响。结果表明亚硒酸钠、烟酰胺、绿茶浸出物和维生素C均能显著降低由香烟烟雾水溶物引起的微核率,上述物质可能具有减轻吸烟引起的遗传毒性损伤的作用。此外,本文还对上述结果的意义进行了讨论。     

利用PCR技术检测孕早期先天性沙眼衣原体感染

先天性感染已成为引起出生缺陷的重要原因,据调查,约有２—８％的新生儿受到先天性感染的影响〔１〕。由风疹病毒等感染引起的先天性感染已逐步为人们所重视,但由沙眼衣原体（Ｃｈｌａｍｙｄｉａｔｒａｃｈｏｍａｔｉｓ,Ｃｔ）引起的先天性感染尚鲜为人知。本研究利用...     

陆地棉洞A核雄性不育系及其MB保持系的遗传模式讨论

本文论述了陆地棉洞A核雄性不育系遗传的不同解释。根据洞A核雄性不育系以及它的不育保持系MB的遗传研究结果,证明洞A核雄性不育系受1对隐性核不育基因控制的结论是正确的,并提出了它的不育保持系MB的遗传本质。     

转导引起基因重组的教学分析

转导引起基因重组的教学分析张霞朱开平（新疆石河子大学师范学院生化系,石河子８３２００３）ＡｎａｌｙｓｉｓｏｆＧｅｎｅｔｉｃＲｅｃｏｍｂｉｎａｔｉｏｎＴｈｒｏｕｇｈＴｒａｎｓｄｕｃｔｉｏｎＺｈａｎｇＸｉａＺｈｕＫａｉｐｉｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＢ...     

三例氨基糖甙类抗生素致聋患者的线粒体DNA测序分析

应用PCR扩增产物直接对3名氨基糖甙类抗生素致聋患者的线粒体DNA进行序 列分析, 结果表明,他们的线粒体DNA均存在第1555位核苷酸A-G的突变。因此认为该突 变是人体对氨基糖甙类抗生素致聋遗传易感性的分子基础,与氨基糖甙类抗生素共同作用造成耳聋。 Abstract:The mitochondria DNAs(mtDNAs)of three patients with AAID were analysed using the method of direct sequencing of their PCR products.The results showed that all their mtDNAs had an A-G mutation at nucleotides 1555.It is considered that this mutation is the molecular basis causing human susceptibility to Aminoglycoside Antibiotics toxicity which in cooperation with Aminoglycoside Antibiotics results in deafness.     

46,XY女性患者SRY基因启动子区域的突变分析

大约15%的46,XY女性患者中发现SRY基因编码区突变,其他患者可能是SRY基因的调节区, 包括启动子区域发生了突变,或者其他相关基因发生突变所致。本文采用限制性酶切、PCR-SSCP及银染检测技术,对7例患者SRY基因的启动子区域进行了突变筛查, 结果未发现异常,提示这些患者的病因与SRY基因启动子区域本身无关,结合对患者SRY基因HMG基序DNA的突变分析结果,表明除SRY基因异常外还存在其他导致46,XY女性性反转综合征的遗传机制。 Abstract:Using restriction endonuelease digestion and PCR-SSCP with silver staining,we analyzed the promotor region of SRY gene in seven 46,XY femalcs.The results showed no abnormality,thus ruling out the mutations in the promotor region of the SRY gene as a possible cause of sex reversal in these XY females.In view with the absence of the mutations in the HMG regions of the SRY genes of several patients,it is suggested that SRY gene is not the only gene responsible for testicular development but is one of many hierarchical genes involved in a genetic cascade for sexual differentiation.     

蚕豆特矮秆突变体的遗传研究

用在国内首次发现的蚕豆特矮秆突变体的优良后代“鄂农 82矮”及高秆品种“启豆1号”为亲本, 研究了株高及其与株高密切有关的两个性状──节间长和节数的遗传。结果表明:株高、节间长、节数3个性状受核基因控制,不存在任何形式的母性遗传。供试双亲株高性状存在一对基因的差异,我们用Df-df表示这一基因座的两个等位基因,高秆亲本基因型为Df Df,矮秆亲本的基因型为dfdf,高秆对矮秆为显性。数量遗传分析表明,3个性状的遗传均符合加性显性模型,株高、节长的加性效应和显性效应都很重要,显性度接近于1。节数仅受加性效应控制,显性效应不显著。 Abstract:DwarF Enong 82,which derived from the unique dwarf mutant discovered in China,was crossed with the commercial broad bean cultivar Qidou 1.From the crossing six basic generations were generated for studing the genetical behaviors of plant height and two related characters i.e. node length and number of nodes.The results showed that the three characters were governed by nuclear genes.The difference in plant height between two parents was related to a loci,which was designated Df-df.Generation mean analyses revealed that the additive-dominance model was adequate to interpreting the inheritance of the three characters.The estimates of number of genes controlling these characterswere the same astheresults with Mendlian methods.