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灿稻品种浙辐802抗瘟性遗传研究 总被引:2,自引:0,他引:2
通过与8个日本鉴别品种杂交和利用日本代表菌系研54-04及我国南方稻区菌系95-t2接种鉴定,研究了灿稻品种浙辐802的抗性遗传。研究结果表明,该品种对菌系研54-04的抗性由2对显性基因控制,对菌系95-t2的抗性由1对显性基因控制。基因等位性分析确认,浙辐802中抵抗95-t2的抗病基因与Pi-i基因等位,与Pi-a、Pi-sh、Pi-k、Pi-z、Pi-b、Pi-t、Pi-ta等已知基因位点 相似文献
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籼稻品种浙辐802抗瘟性遗传研究 总被引:7,自引:0,他引:7
通过与8个日本鉴别品种杂交和利用日本代表菌系研54-04及我国南方稻区菌系95-h接种鉴定,研究了籼稻品种浙辐802的抗性遗传。研究结果表明,该品种对菌系研54-04的抗性由 2对显性基因控制,对菌系 95-t2的抗性由 1对显性基因控制。基因等位性分析确认,浙辐802中抵抗95-t2的抗病基因与Pi-i基因等位,与Pi-a、Pi-sh、Pi-k、Pi-z、Pi-b、Pi-t、Pi-ta等已知基因位点为非等位关系;抵抗菌系研54-04,感染菌系95-t2的基因与Pi-i、Pi-k、Pi-b、 Pi-t 4个已知基因位点为非等位关系。对这个基因与其他已知基因的等位性进行了分析,认为它可能是1个未被命名的新基因。 相似文献
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太湖流域粳稻地方品种黑壳子粳对稻瘟病抗性的遗传分析 总被引:6,自引:0,他引:6
太湖流域粳稻地方品种黑壳子粳对稻瘟病菌表现抗谱广,抗性强的特点,利用黑壳子粳与感病的云南稻地方品种丽江新团黑谷杂交获得的F1、F2和RIL群体,在苗期喷雾接种研54-04和北1两个日本稻瘟病鉴别菌系,根据抗感反应分析亲本的抗病基因组成,结果表明,黑壳子粳对菌系北1的抗性由一对显性基因控制,对菌系研54-04的抗性由两对互为独立遗传的显性基因控制,等位性测定结果和重组自交系的抗感反应表明:黑壳子粳对菌系北1的抗病基因兼抗菌系研54-04,该抗病基因与Pi-k,Pi-z,Pi-ta,Pi-b,Pi-t等5个已知抗病基因座呈非等位关系。也不是Pi-i和Pi-a基因,推断是一个未知的新基因;另一个抗病基因抗菌系研54-04,感菌系北1。 相似文献
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与小麦白粉病抗性基因Pm2紧密连锁RAPD标记的筛选研究 总被引:8,自引:0,他引:8
以256个随机引物对含小麦抗白粉病基因Pm2近等基因系进行RAPD分析,发现17个随机引物的扩增产物在抗、感NILs材料间表现多态性,且其中5个引物经4次以上重复,均获相同结果,其多态性标记分别为OPM08(1600)、OPI04(1700)、OPH19(1100、OPE09(900)及OPM16(850)。当以这5个随机引物对14个已知含Pm2基因的抗病材料及9个不含Pm2基因的感病材料进行检测时,只有标记OPI04(1700)在12个抗病材料中出现(另两个抗病材料中未检测到),而在9个感病材料中均未出现。进一步用 OPI(04)对102株(Chancellorx Uka/8*Cc)F2分离群体进行分析,估算出标记OPI04(1700)与Pm2基因间的遗传距离为12.2±3.3cM。 相似文献
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水稻分蘖角度的QTLs分析 总被引:19,自引:5,他引:14
分蘖角是水稻株型构成的重要性状之一,在育种上人有极其重要的意义,利用分蘖角度差异显著的1对籼粳亲本,将其杂交F1代花培加倍,构建了1个DH群体,考察了115个DH株系的分蘖角度,并使用该群体构建的分子图谱进行数量性状座痊(QTLs)分析。分别在第9和12个染色体上检测3个QTLs(qTA-9a、qTA-9b和qTA-12),贡献率分别为22.7%、11.9%和20.9%,其加性效应均为负,表明由分蘖角度较大的窄占青8号的基因控制,并讨论了这种由主产和微效基因控制的分蘖性状在育种学上的应用。 相似文献
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栽培稻F1花粉不育基因座S—a的分子定位 总被引:9,自引:0,他引:9
以栽培稻品种台中65及其等基因F1不育系TISL4为材料,用RFLP和RAPD等技术,对F1花粉不育基因座S-a定位。通过用RFLP和RAPD方法对亲本间进行多态性分析,发现亲本间的多态性很低,说明经多代回交后,在等基因系基因组中供体亲本的DNA片段所占的比例很小。通过连锁分析,将S-a定位在第1染色体。S-a与分子标记CDO548、O11-1000、RG146和Y13-500之间的遗传距离分别为 相似文献
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一个新的水稻迟熟性基因的遗传分析和分子标记定位 总被引:9,自引:1,他引:8
中籼迟熟水稻品系8987含未知的长生育期基因,在杂交水稻育种中有重要的利用价值,应用该品系与4个不同生态类型的水稻品种杂交,对其F1和F2群体进行生育期调查和遗传分析,确认8987的长生育期受1对隐性主效基因控制。以(8987X地谷)F2群体为基础,应用RFLP和微卫星标记结合群分法,发现第7染色体的RFLP标记C213与该基因连锁;进一步应用F2分离群体将该基因定位于第7染色体上,暂定名为lf-3。此基因的发现和定位将有助于分子标记辅助选择和杂交水稻的改良。 相似文献
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A synthesis of roseoflavin (RoF) by Streptomyces davawensis from 8-amino- (AF) and 8-methylamino-8-demethyl-D-riboflavin (MAF) was demonstrated. The lines of evidence are: 1) the RoF formation was increased by addition of AF or MAF to the culture, 2) [2-14C]RoF was formed by addition of [2-14C]AF or [2-14C]MAF to the culture, and the location of the 14C atom at the 2-position was demonstrated by identifying [14C]urea in the hydrolysate of the RoF, 3) [N-methyl-14C]RoF was formed by addition of [methyl-14C]methionine to the culture containing AF or MAF, and the location of the 14C atom was confirmed by the photochemical conversion of the RoF to MAF, the specific radioactivity of which was about half that of the original RoF, and by localization of the 14C atom in 1,2-dihydro-6-methyl-7-dimethylamino-2-keto-1-ribityl-3-quinoxalinecarbo xylic acid (QC), which was formed from the RoF by hydrolysis. 相似文献
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Partial trisomy 8 (8q24) and the trisomy-8 syndrome 总被引:5,自引:0,他引:5
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Isolation and structural characterisation of 8-O-4/8-O-4- and 8-8/8-O-4-coupled dehydrotriferulic acids from maize bran 总被引:3,自引:0,他引:3
Two new dehydrotriferulic acids were isolated from saponified maize bran insoluble fiber using Sephadex LH-20 chromatography followed by semi-preparative RP-HPLC. Based on UV-spectroscopy, mass spectroscopy and one- and two-dimensional NMR experiments, the structures were identified as 8-O-4,8-O-4-dehydrotriferulic acid and 8-8(cyclic),8-O-4-dehydrotriferulic acid. Which of the possible phenols in the initially formed 8-8-dehydrodiferulate was etherified by 4-O-8-coupling with ferulate has been unambiguously elucidated. The ferulate dehydrotrimers which give rise to these dehydrotriferulic acids following saponification are presumed, like the dehydrodiferulates, to cross-link polysaccharides. Neither dehydrotriferulic acid described here involves a 5-5-dehydrodiferulic acid unit; only the 5-5-dehydrodimer may be formed intramolecularly. However, whether dehydrotriferulates are capable of cross-linking more than two polysaccharide chains remains open. Although the levels of the isolated ferulate dehydrotrimers are lower than those of the ferulate dehydrodimers, the isolation now of three different dehydrotriferulates indicates that trimers contribute to a strong network cross-linking plant cell wall polysaccharides. 相似文献
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Tissue stress (TS) is defined as the stress which acts on atissue layer in an organ in excess of the turgor-induced tensilestress which also acts on the layer when it is isolated fromthe organ. The tensile TS in one layer of an organ is alwaysaccompanied by com-pressive TS in another layer. To calculateTS from data obtained for isolated tissue, one needs to knowthe Poisson ratios VIKcontraction in the k-direction/extension in the i-direction for the tensile force applied in the i-direction for that tissue.Poisson ratios apply in the relationships between (i) tissueextensions caused by uniaxial stress (due to applied force)and multiaxial stress (due to turgor pressure); (ii) extensionsof tissues subjected to lateral constraint, and (iii) TSs indifferent directions. The ratios VIW and VWI, for the stressapplied either longitudinally (I) or in the direction of width(W), respectively, were determined for the outer tissue (OT)of sunflower hypocotyls, tulip leaves and tulip stems. The tworatios for a particular OT differed considerably. The ratiosdepended on the applied extension (strain). Knowing them, thetensile force (F1) generating TS in the OT of an intact organcould be calculated from the longitudinal force (FI(s)) whichwhen applied to the isolated (unconstrained laterally) OT restoredits original length. In the case of the sunflower hypocotyls,FI(s)<F1<1.3 FI(s). The ratio VIr (r denotes the radialdirection), which was determined for segments of inner tissue,from sunflower hypocotyls and tulip stems, did not depend onthe applied stress (extension). This ratio allowed us to calculatethe relationship between the strain changes caused by equalchanges of uniaxial and multiaxial stresses: the uniaxial stresswas approximately 3-fold more efficient than the multiaxialstress. Key words: Elastic strains, Poisson ratios, tissue stresses, tissue elasticity, uni- and multiaxial stresses. 相似文献
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