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1.
【目的】锡兰玻壳蚧是我国余甘子上新发现的重要害虫,对农业生产及生态安全构成严重威胁。本研究旨在筛选出高效理想的防治药剂,为生产中科学用药提供依据。【方法】采用浸渍法测定8种杀虫剂对锡兰玻壳蚧的室内毒力,采用喷雾法评价5种毒力较好药剂的田间防效。【结果】室内毒力测定结果表明:毒死蜱、啶虫脒、吡虫啉、阿维菌素、甲维盐对1、2龄若虫的毒力较强,48 h致死中浓度(LC50)分别为11.1245、26.2376 mg·L-1,17.5381、26.1910 mg·L-1,27.5896、34.2868 mg·L-1,26.5876、38.8034 mg·L-1,29.0528、43.0443 mg·L-1;毒死蜱和阿维菌素对3龄若虫、雌成虫的毒力较强,48 hLC50分别为87.3427、495.8339 mg·L-1,95.4751、1131.6430 mg·L-1;螺虫乙酯、吡丙醚和高效氯氟氰菊酯对各虫期的毒力均较弱;吡虫啉、阿维菌素、啶虫脒、甲维盐对各龄若虫的毒力比差异较小,毒死蜱的毒力比差异较大,但后者对各龄若虫的毒力均较强。田间防效结果表明:毒死蜱和阿维菌素对田间种群增长抑制效果最好,药后7 d防效分别为96.90%、92.00%;吡虫啉、甲维盐和啶虫脒对低龄若虫种群速杀效果较好,药后7 d防效分别为78.06%、67.62%、62.78%。【结论】锡兰玻壳蚧的化学防治应于1~2龄若虫期进行,推荐阿维菌素、毒死蜱、吡虫啉、甲维盐和啶虫脒轮换使用;3龄雌若虫及雌成虫盛发期推荐使用毒死蜱和阿维菌素防治。  相似文献   

2.
[目的]研究皂荚生物农药活性,开发利用皂荚资源,发展环境友好的绿色植物源农药。[方法]采用室内生测和田间试验研究皂荚壳乙醇提取物的杀螺活性。[结果]皂荚提取物对福寿螺有显著的毒杀活性,对幼螺和成螺72 h的LC50分别为40.56、109.83 mg·L-1。田间试验表明,皂荚提取物对福寿螺有较好的防效,施用40 g·m-2的皂荚提取物处理7 d后卵块减少率为100.00%(成螺失去产卵的能力),防效为(99.12±1.26)%。[结论]皂荚提取物对福寿螺较好的生物防治效果,是一种潜在的生物杀螺剂。  相似文献   

3.
陈瑶  周寒梅  何兵  李维 《广西植物》2020,40(11):1681-1690
为探明华重楼离体胚培养及植株再生的基本体系,该文以华重楼离体幼胚为试验材料,以MS培养基为基本培养基,研究不同光照、不同浓度梯度组合的植物生长调节剂对华重楼离体幼胚萌发、成苗的影响。结果表明:培养到60 d时,暗培养条件下华重楼离体幼胚的生长率和萌发率分别比光培养条件下高45.25%、19.17%,故暗培养比光培养更有利于华重楼离体幼胚生长发育。当GA3浓度相同时,离体幼胚萌发所需时间随IAA浓度增加而延长。不同浓度的GA3都可促进离体幼胚萌发,促进作用由强到弱依次为5 mg·L-1 GA3>1 mg·L-1 GA3> 10 mg·L-1 GA3。在黑暗条件下,优选得到最适华重楼离体幼胚生长发育配方为1/2 MS+30 g·L-1 蔗糖+7 g·L-1琼脂+0.5 g·L-1 活性炭+5 mg·L-1GA3+1 mg·L-1 IAA。此配方可诱导华重楼离体幼胚在2个月左右萌发,萌发率达50%需80 d左右。在华重楼成熟胚出苗培养时发现,高浓度的植物生长调节剂会抑制华重楼成熟胚生长发育,高浓度GA3甚至会导致华重楼成熟胚死亡。优选得到最适华重楼成熟胚出苗配方为MS+30 g·L-1 蔗糖+6.2 mg·L-1 硼酸+7 g·L-1 琼脂+0.7 g·L-1 活性炭+0.5 mg·L-1 2,4-D+1.5 mg·L-1 IAA + 1.5 mg·L-1 ZT+5mg·L-1 GA3,诱导成熟胚出苗需43 d左右,75 d左右可形成真叶。  相似文献   

4.
以‘全年油麦菜’尖叶莴苣为试验材料,采用水培方式,研究3个浓度(0 mg·L-1、0.1 mg·L-1、1 mg·L-1)Ni2+在22.4 mg·L-1 N处理下对尖叶莴苣氮素吸收的生长及生理影响。结果显示:(1)尖叶莴苣根系和地上部生物量随处理时间的增加呈上升趋势。与对照T1(0 mg·L-1 Ni2+、112 mg·L-1 N)相比,T2处理(0 mg·L-1 Ni2+、22.4 mg·L-1 N)对尖叶莴苣根系及叶片生长具有一定抑制作用,植株鲜重、干重、根冠比、根系长度、平均直径、表面积、体积、根尖数、分根数、叶片表面积和体积在T3处理(0.1 mg·L-1 Ni2+、22.4 mg·L-1 N)下显著高于对照,T4处理(1 mg·L-1 Ni2+、22.4 mg·L-1 N)对尖叶莴苣根系及其叶片生长具有一定促进作用,但对其根尖数和分根数表现出一定抑制性。(2)随着Ni2+浓度的增加,尖叶莴苣叶片叶绿素a、叶绿素b和总叶绿素含量呈先升后降的变化规律,且均在T3处理下显著提高。(3)随着处理时间的增加,尖叶莴苣叶片的净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)逐渐上升,胞间CO2浓度(Ci)逐渐下降,且T3处理叶片的Gs显著高于对照,其Ci最低,Pn最大。(4)施加Ni2+对尖叶莴苣有机酸、可溶性蛋白和可溶性糖含量以及SOD和POD活性有显著影响,在T3处理下有机酸含量降低,可溶性糖和可溶性蛋白含量显著增加,SOD和POD活性显著提高。(5)T3处理尖叶莴苣根系中N及叶片中B和Ca含量较高;根系中Ni含量高于叶片,T3处理叶片中的Ni含量较低,Mg含量较高;植株体内Cu含量随Ni2+浓度增加而下降。研究表明,外源Ni2+处理能影响低氮条件下(22.4 mg·L-1 N)尖叶莴苣幼苗生长及生理状况,适宜浓度(0.1 mg·L-1)Ni2+可有效提高尖叶莴苣根系对氮素的吸收利用效率,减少氮素施用量,促进尖叶莴苣根系和地上部叶片生长,增加光合色素含量,并提高净光合速率,进而改善植株的产量和营养品质。  相似文献   

5.
厌氧氨氧化菌混培物生长及代谢动力学研究1   总被引:4,自引:0,他引:4  
研究了厌氧氨氧化菌混培物的动力学特性。测得细胞产率系数1.573mgVS(mmol NH+4)-1;细胞衰减常数0.052mgVS(g·VS·d)-1。厌氧氨氧化菌混培物的最大氨氧化速率1.320~2.761mmol(gVS·d)-1,最大亚硝酸盐转化(反硝化)速率14.497mmol(gVS·d)-1。厌氧氨氧化菌混培物利用氨的Km值1.801~4.215mmol·L-1,利用亚硝酸盐的Km值0.468mmol·L-1。氨自身的抑制常数38.018~98.465mmol·L-1,实际最大氨氧化速率的氨浓度16.656mmol·L-1。亚硝酸盐对厌氧氨氧化的抑制常数5.401~11.995mmol·L-1。厌氧氨氧化的最适pH7.605。厌氧氨氧化的最适温度30℃。Vmaxa、Kma、Kia和Kin的活化能依次为37.316、30.239、33.695和30473kJ·mol-1。  相似文献   

6.
杨岚  师帅  向增旭 《西北植物学报》2013,33(11):2189-2193
以铁皮石斛原球茎为材料,经不同质量浓度的秋水仙素(C22H25O6)和0.02 g·mL-1二甲基亚砜(DMSO)混合水溶液处理后进行组织培养,通过对变异株进行形态学、细胞学及流式细胞仪鉴定,以期获得稳定的四倍体植株并分析其生理特性。结果表明:用2.0 g·L-1秋水仙素和0.02 g·mL-1 DMSO混合水溶液处理铁皮石斛原球茎36 h后,植株诱导率达20%;诱导四倍体植株在形态上明显矮化、茎秆粗壮、叶片变小增厚、气孔直径增大;细胞遗传学观察发现,四倍体植株染色体2n=4x=76,二倍体植株染色体2n=2x=38;流式细胞仪分析显示,DNA相对含量四倍体为400,二倍体仅为200;四倍体植株叶片中叶绿素含量、可溶性蛋白、可溶性糖含量均高于二倍体,分别为5.03、3.59、2.98 mg·g-1;四倍体叶片中主要抗氧化酶POD和SOD活性均显著高于二倍体,分别为9.08、180.4 U·mg-1,且四倍体植株明显降低了MDA含量累积。研究认为,2.0 g·L-1秋水仙素和0.02 g·mL-1 DMSO混合水溶液处理原球茎36 h可提高诱导成功率、降低嵌合体比例,此组合为诱导四倍体较佳诱导条件。  相似文献   

7.
【目的】为生产中应用昆虫病原线虫防治草地贪夜蛾提供参考。【方法】采用室内实验和田间试验相结合的方法,室内培养皿中测定4种不同品系线虫对3和6龄草地贪夜蛾幼虫的毒力、致死时间和剂量,田间玉米6~8叶期、日落后叶面喷施线虫至喇叭口内,测试防治草地贪夜蛾的施用剂量和持效天数。【结果】Steinernema feltiae SN、Heterorhabditis bacteriophora H06、Heterorhabditis beicherriana Cherry和Oscheius chongmingensis Tumian侵染草地贪夜蛾3龄幼虫72 h后的致死中浓度LC50分别为22.13、36.54、60.01和1369.4 IJs·larva-1;侵染6龄幼虫72 h后的致死中浓度LC50分别为18.67、31.25、45.52和897.28 IJs·larva-1。SN、H06、Cherry和Tumian品系的最佳致死时间72 h;最佳致死剂量100 IJs·larva-1 (Tumian品系除外,最佳致死剂量为900 IJs·larva-1)。玉米田线虫施用剂量7万IJs·株-1为最佳,施用后16 d的校正虫口减退率为65.80%。【结论】在供试品系中,S. feltiae SN品系的线虫对草地贪夜蛾的致病力最强,最有田间生物防治草地贪夜蛾潜力。田间玉米6~8叶期、幼虫3龄时喷施线虫至喇叭口内防治效果最佳。  相似文献   

8.
[目的]研究6种杀虫剂对南美番茄潜叶蛾卵、幼虫和成虫的毒力及其在温室番茄上的防治效果,为南美番茄潜叶蛾防治提供高效杀虫剂和施药技术。[方法]采用浸渍法和药膜法测定了6种杀虫剂对南美番茄潜叶蛾卵、幼虫和成虫的毒力,田间调查毒力较高杀虫剂对温室番茄上南美番茄潜叶蛾防效。[结果] 6种杀虫剂中的乙基多杀菌素、甲氨基阿维菌素苯甲酸盐和阿维菌素对南美番茄潜叶蛾卵有毒力作用,致死中浓度(LC50)分别为1.415、13.588和23.194 mg·L-1。6种杀虫剂对南美番茄潜叶蛾幼虫的LC50分别为:阿维菌素0.026 mg·L-1、四唑虫酰胺0.052 mg·L-1、甲氨基阿维菌素苯甲酸盐0.057 mg·L-1、乙基多杀菌素0.072 mg·L-1、氯虫苯甲酰胺0.484 mg·L-1和呋虫胺2.039 mg·L-1。对于南美番茄潜叶蛾成虫,24 h时,仅甲氨基阿维菌素苯甲酸盐和四唑虫酰胺对成虫有较高毒力;72 h时,6种杀虫剂对成虫的LC50分别为:甲氨基阿维菌素苯甲酸盐0.390 mg·L-1、乙基多杀菌素1.646 mg·L-1、四唑虫酰胺2.630 mg·L-1、呋虫胺5.577 mg·L-1、阿维菌素22.502 mg·L-1和氯虫苯甲酰胺39.636 mg·L-1。在成虫盛发期第4天施药,阿维菌素、四唑虫酰胺、甲氨基阿维菌素苯甲酸盐、乙基多杀菌素在南美番茄潜叶蛾危害严重的温室番茄上防效达80%以上。[结论]阿维菌素、四唑虫酰胺、甲氨基阿维菌素苯甲酸盐、乙基多杀菌素、氯虫苯甲酰胺和呋虫胺对南美番茄潜叶蛾卵、幼虫或成虫有较高毒力,其中阿维菌素、四唑虫酰胺、甲氨基阿维菌素苯甲酸盐和乙基多杀菌素田间防效较好。  相似文献   

9.
膜-生物硝化反应器处理含氨废水效能的研究   总被引:1,自引:0,他引:1  
武小鹰  郑平  胡宝兰   《生物工程学报》2005,21(2):279-283
研究了膜 生物硝化反应器对含氨废水的处理效能以及分离膜的截留和渗透效能。膜_生物反应器启动迅速 ,在水力停留时间为 1d的情况下 ,反应器最高进水浓度达 80mmol(NH4+-N)·L-1 ,最高容积负荷达 1 12kg(NH4+ -N)·m-3·d-1 ,氨氮去除率保持在 95%以上。试验证明 ,分离膜对微生物有良好的截留作用 ,50天内反应器的污泥浓度从 5g·L-1 增长到 10g·L-1 ,分离膜表面附着的生物层则对废水氨氮和亚硝氮有进一步的转化作用。在液位差低于 80cm时 ,提高液位差可增大膜渗透通量 ;液位差超过 80cm后 ,增大液位差的膜渗透通量效应很小 ;其中 ,当液位差为 2 0cm左右时 ,膜通量达 2 . 5 1L·m-2 ·h-1 ,阻力最小 [(2 . 6 3× 10-5)m-1]。该膜_生物硝化反应器可依靠液位差压力驱动出水 ,无需外加动力。  相似文献   

10.
刘洋  王玉英  李枝林  李茹 《广西植物》2019,39(10):1327-1333
该研究以大花蕙兰‘红酒''(Cymbidium hybridum ‘hongjiu'')×莲瓣兰‘边草素花''(C. tortisepalum ‘biancaosuhua'')F1代杂交种原球茎和根状茎为材料,比较了不同激素配比增殖分化、生根的培养基,建立了适用杂交兰组培快繁体系。结果表明:1/2MS+6-BA 1.0 mg·L-1+NAA 1.0 mg·L-1+AC 0.05%+香蕉80 g·L-1对原球茎增殖效果最佳,增殖率达到307%; 1/2MS+6-BA 1.5 mg·L-1+NAA 1.0 mg·L-1 +AC 0.05%+香蕉80 g·L-1有利于原球茎分化,分化率为82%; 1/2MS+TDZ 2.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.05%+香蕉80 g·L-1对根状茎增殖分化效果最佳,增殖率为293%,分化率为79%; 1/2MS+IBA 0.5 mg·L-1+NAA 0.3 mg·L-1+AC 0.05%+香蕉80 g·L-1为最佳生根培养基,生根率达到84.7%,且根粗苗壮,叶色浓绿。此体系为杂交兰种苗的规模化生产提供了技术支持。  相似文献   

11.
Scirtothrips perseae Nakahara was discovered attacking avocados in California, USA, in 1996. Host plant surveys in California indicated that S. perseae has a highly restricted host range with larvae being found only on avocados, while adults were collected from 11 different plant species. As part of a management program for this pest, a “classical” biological control program was initiated and foreign exploration was conducted to delineate the home range of S. perseae, to survey for associated natural enemies and inventory other species of phytophagous thrips on avocados grown in Mexico, Guatemala, Costa Rica, the Dominican Republic, Trinidad, and Brazil. Foreign exploration efforts indicate that S. perseae occurs on avocados grown at high altitudes (>1500 m) from Uruapan in Mexico south to areas around Guatemala City in Guatemala. In Costa Rica, S. perseae is replaced by an undescribed congener as the dominant phytophagous thrips on avocados grown at high altitudes (>1300 m). No species of Scirtothrips were found on avocados in the Dominican Republic, Trinidad, or Brazil. In total, 2136 phytophagous thrips were collected and identified, representing over 47 identified species from at least 19 genera. The significance of these species records is discussed. Of collected material 4% were potential thrips biological control agents. Natural enemies were dominated by six genera of predatory thrips (Aeolothrips, Aleurodothrips, Franklinothrips, Leptothrips, Scolothrips, and Karnyothrips). One genus each of parasitoid (Ceranisus) and predatory mite (Balaustium) were found. Based on the results of our sampling techniques, prospects for the importation of thrips natural enemies for use in a “classical” biological control program in California against S. perseae are not promising.  相似文献   

12.
Parsimony analyses of the internal transcribed spacer regions of nuclear ribosomal DNA (ITS 1 & ITS 2) for 38 taxa sampled from the Phebalium group (Rutaceae: Boronieae) and two outgroups confirm that, with the exception of Phebalium sensu stricto and Rhadinothamnus, six of the currently recognised genera within the group are monophyletic. The data indicate that Phebaliums. str. is paraphyletic with respect to Microcybe, and Rhadinothamnus is paraphyletic with respect to Chorilaena. Rhadinothamnus and Chorilaena together are the sister group to Nematolepis. Drummondita, included as an outgroup taxon, clustered within the ingroup as sister to Muiriantha and related to Asterolasia.The phylogeny suggests that the evolution of major clades within a number of these genera (e.g. Phebalium) relates to vicariance events between eastern and south-western Australia. Leionema is an eastern genus, with the most basal taxon being the morphologically distinct Leionema ellipticum from northern Queensland. Leionema also includes one species from New Zealand, but this species (as with some others) proved difficult to sequence and its phylogenetic position remains unknown. Taxonomic changes at the generic level are recommended.The authors wish to thank Paul G.Wilson, PERTH, for advice and discussion, and Paul Forster, BRI, for collecting and providing material of Leionema ellipticum. The project was supported by a Melbourne University Postgraduate Award (to BM), the Australian Biological Resources Study (ABRS), Australian Systematic Botany Society and Wolf Den (Australia) Investments.  相似文献   

13.
Komárek has recently reviewed the various species assigned to the green algal genusNeochloris Starr (Chlorococcales, Chlorococcaceae) and removed those with uninucleate vegetative cells to a new genus,Ettlia. Watanabe & Floyd, unaware ofKomárek's work, also reviewed the species ofNeochloris and distributed them among three genera—Neochloris, Chlorococcopsis gen. nov., andParietochloris gen. nov.—on the basis of details of the covering of the zoospore and the arrangement of the basal bodies of the flagellar apparatus. This paper reconciles these two treatments and makes additional recommendations at the ranks of genus, family, order, and class.  相似文献   

14.
15.
seventeen new species and combinations are proposed in the generaChondrorhyncha, Cischweinfia, Cochlioda, Eloyella, Encyclia, Kefersteinia, Koellensteinia, Macroclinium, Rodriguezia, Solenidiopsis, andStenia. All new species are illustrated. A key is provided for 2-flowered species ofMacroclinium, PeruvianSigmatostalix, and PeruvianStenia. Solenidium (Solenidiopsis) peruvianum Schltr. is lectotypified.  相似文献   

16.
The genusKarschia, in the earlier sense, including saprophytes and parasites on lichens, has been thought to be a non-lichenized parallel genus of the lichen genusBuellia. Modern workers included it on the one hand inBuellia, on the other hand combined it with bitunicate ascomycetes. It is now proved thatKarschia is heterogeneous and contains but superficially similar members both of the genusBuellia of theLecanorales and of typical or masked bitunicateAscomycetes. Therefore, it can not be regarded as a link betweenLecanorales andDothideales. The type species ofKarschia belongs to theDothideales.
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17.
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.
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18.
The family Sordariaceae incorporates a number of fungi that are excellent model organisms for various biological, biochemical, ecological, genetic and evolutionary studies. To determine the evolutionary relationships within this group and their respective phylogenetic placements, multiple-gene sequences (partial nuclear 28S ribosomal DNA, nuclear ITS ribosomal DNA and partial nuclear β-tubulin) were analysed using maximum parsimony and Bayesian analyses. Analyses of different gene datasets were performed individually and then combined to generate phylogenies. We report that Sordariaceae, with the exclusion Apodus and Diplogelasinospora, is a monophyletic group. Apodus and Diplogelasinospora are related to Lasiosphaeriaceae. Multiple gene analyses suggest that the spore sheath is not a phylogenetically significant character to segregate Asordaria from Sordaria. Smooth-spored Sordaria species (including so-called Asordaria species) constitute a natural group. Asordaria is therefore congeneric with Sordaria. Anixiella species nested among Gelasinospora species, providing further evidence that non-ostiolate ascomata have evolved from ostiolate ascomata on several independent occasions. This study agrees with previous studies that show heterothallic Neurospora species to be monophyletic, but that homothallic ones may have a multiple origins. Although Gelasinospora and Neurospora are closely related and not resolved as monophyletic groups, there is insufficient evidence to place currently accepted Gelasinospora and Neurospora species into the same genus.  相似文献   

19.
Little is known about how tropical land-use systems contribute to the conservation of functionally important insect groups, including dung beetles. In a study at the margin of Lore Lindu National Park (a biodiversity hotspot in Central Sulawesi, Indonesia) dung-beetle communities were sampled in natural forest, young secondary forest, agroforestry systems (cacao plantations with shade trees) and annual cultures (maize fields), each with four replicates (n = 16 sites). At each site we used 10 pitfall traps, baited with cattle dung, along a 100 m transect for six 3-day periods. The number of trapped specimens and species richness at the natural forest sites was higher than in all land-use systems, which did not significantly differ. Each land-use system contained, on average, 75% of the species richness of the natural forest, thereby indicating their importance for conservation. However, a two-dimensional scaling plot based on NESS indices (m = 6) indicated distinct dung beetle communities for both forest types, while agroforestry systems and annual cultures exhibited a pronounced overlap. Mean body size of dung beetles was not significantly influenced by land-use intensity. Five of the six most abundant dung beetle species were recorded in all habitats, whereas the abundance of five other species was significantly related to habitat type. Mean local abundance and number of occupied sites were closely correlated, further indicating little habitat specialisation. The low dung beetle diversity (total of 18 recorded species) may be due to the absence of larger mammals in Sulawesi during historical times, even though Sulawesi is the largest island of Wallacea. In conclusion, the dung beetle fauna of the lower montane forest zone in Central Sulawesi appears to be relatively robust to man-made habitat changes and the majority of species did not exhibit strong habitat preferences.  相似文献   

20.
郭英兰 《菌物学报》1991,10(Z1):119-128
本文报导作者采自安徽枯牛降自然保护区的18种丝孢菌,分属于5个属,其中有3个新种:牛皮冻生尾孢(Cercospora paederiicola),山鸡椒假尾饱(Pseudocercospora litseae-cubebae),鸡血藤生假尾孢(P. millettiicola)和2个中国新纪录。文中对新种进行了描述及绘图,新记录种作了简要说明。研究的标本保存在中国科学院微生物研究所真菌标本室(HMAS)。  相似文献   

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