青少年白发的皮纹学研究

本文对203名青少年白发患者的皮纹进行了分析,并与216名正常对照组个体作了比较.发现青少年白发组的As和Wd出现率以及A/A和L/W的对应频率均显著低于正常对照组;青少年白发组女性的a-bTRC值、男性变异型掌褶纹的出现率、10指同斗频率、右手Ⅰ2区及左手Hy区的花纹出现率均显著高于正常对照组的相应出现率.说明青少年白发者的皮纹可能有自身的遗传特征。 Abstract:This paper sought to analyze the dermatoglyph of 203 cases with juvenile poliosis,and make an individual contrast with the control group of 216 normal juveniles.It was found that the incidence of As and Wd in the group of juvenile poliosis and the correspondence rate of A/A and L/W were obviously lower than those of the normal contrast group,while a-b TRC value in the female patients with juvenile poliosis and the incidence of abnormal palmprint in the male, the frequency of all loop whorl,and palm patterns in the I2 area of the right hand and in the Hyarea of the left hand were evidently higher than the corresponding incidences of the normal contrast group.Dermatoglyph of the juvenile poliosis has its own heredity.     

肿瘤患者皮纹特征的量化分类初步分析

本文对肿瘤患者的指纹和掌纹按照标准的分类方法进行了较为详细的分析,并与对照组进行了对比,用χ2检验的方法,发现患者与对照组之间在指纹和掌纹的出现率上均存在差异,这些结论为搭建量化皮纹特征模型、建立量化的皮纹特征指标体系、患者与对照组的分类判别都提供了有力的理论依据。     

抑郁症患者的皮纹特征

采用体质测量法以天津汉族正常者310例(女性231例, 男性79例)为对照, 分析天津汉族抑郁症患者202例(女性140例, 男性62例)的皮纹特征。经统计学分析, 结果显示: 抑郁症患者组双手a-b嵴线数、小鱼际及各指间区真实花纹出现率较正常组无显著性差异; 指端斗形纹出现率增加(P<0.01); 掌部a-b嵴线数波动不对称性(FA值)增加(P<0.01)、atd角偏大(P<0.01)、桥贯型及皱褶掌出现率增加(P<0.01)等可能是抑郁症患者的主要皮纹特征。     

先天性心脏病患者皮纹波动不对称性的研究

本文采用随机整体抽样的方法分析了先天性心脏病患者129例(男性59例, 女性70例) 和正常对照人群133例(男性69例, 女性64例) 13项皮纹波动不对称性(Fluctuating asymmetry, FA)的分布特征。结果表明: (1)先天性心脏病患者组与正常对照组在13项皮纹波动不对称性指标中均未出现显著性别差异; (2)先天性心脏病患者组与正常对照组在FAⅥ(P<0.05)和FAⅦ(P<0.01)两项有显著性差异, 表现为患者组明显增高, 提示先天性心脏病患者在胚胎发育早期易受到环境因素影响, 具有较高的发育不稳定性。     

多巴胺D1受体基因多态性与注意缺陷多动障碍的相关性研究

目的:探讨多巴胺D1受体(Dopamine D1 receptor, DRD1)基因启动子区G-48A、外显子区T1403C两个SNP位点与注意缺陷多动障碍(attention deficit hyperactivity disorder,ADHD)的关联性.方法:选取87名ADHD患者和103名正常对照,提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测G-48A和T1403C两个多态性位点的基因型,SPSS13.0软件分析各位点的等位基因及基因型频率.结果:DRD1的G-48A基因型及等位基因频率分布在ADHD和对照组之间有统计学差异(p<0.05),ADHD组中等位基因A频率显著高于正常组(p<0.05).T1403C位点基因型及等位基因频率在ADHD组与健康对照组无统计学差异.结论:DRD1基因G-48A多态性可能与ADHD的发病有关,携带有等位基因A的个体可能更容易患ADHD.T1403C多态性与ADHD的发病无明显相关性.     

湖南地区HBV患者MHC—I类链相关基因A第五外显子微卫星多态性研究

分别提取湖南汉族人群108例慢性乙肝病毒（hepatitis B virus,HBV）患者、96例HBV携带者和142例健康对照者外周血基因组DNA,利用聚合酶链反应和基因扫描技术分别对它们的主要组织相容性复合体I类链相关A（major histocompatibility complex class I chain related A, MICA）基因第5外显子进行微卫星多态性分析;应用DNA测序分析对不同的基因型进行验证,同时应用PCR／SSP技术进行MICA＊Del检测,确定MICA基因第5外显子基因型。发现本研究的三组中分别检出44、A5、A5．1、A6、A9五种等位基因,且以A5和A5．1为主;在HBV携带组和健康对照组中分别检出MICA＊Del基因。结果同时显示慢性HBV患者组MICA＊A5．1／A9基因型频率、慢性HBV患者组的MICA＊A9等位基因频率、慢性HBV患者组MICA＊A9表型频率和HBV携带组MICA＊A5．I／A9基因型频率均低于相应的健康对照组;而湖南地区汉族人群HBV携带者和慢性HBV患者问的基因型频率、等位基因频率和表型频率无显著性差异;因而推测MICA＊A5．1／A9基因型和MICA$A9等位基因可能是抗HBV感染的一种保护性等位基因。为今后进一步研究HBV的感染、预防和治疗提供参考依据。     

白介素10和白介素17基因启动子区多态性位点与儿童哮喘的相关性研究

目的：探讨IL-10基因启动子区-627A/C和IL-17基因启动子-152A/G位点多态性与儿童哮喘发生的相关性。方法：采用聚合酶链反应-限制性片段长度多态性分析（PCR-PFLP）方法检测186名哮喘儿童、198名健康儿童各个多态性位点的基因型,采用SPSS13.0进行统计学分析。结果：IL-17基因-152A/G位点的基因型及等位基因频率分布在哮喘组与正常对照组均存在显著性差异（p〈0.05）,哮喘组-152A/G位点等位基因A频率显著高于正常对照组（x2=6.077,p=0.014,OR=1.430,95%CI=1.076-1.902）。结论：IL-17基因-152A/G位点可能与儿童哮喘的发病存在关系,其中A等位基因可能是易感基因,携带A的个体可能更易患有哮喘。     

三色荧光原位杂交技术（Triple—FISH）检测男性不育患者精子染色体的非整倍体性

目的：研究精子非整倍体染色体与男性不育之间的关系。方法：用荧光原位杂交（FISH）技术,对12个不育患精子的染色体进行了研究,其中,10例少,弱,畸精症患为A组,2例正常或接近正常的样品为B组,另有2例正常健康献精为对照组,原位杂交采用染色体XY和18号探针,每个个体记数1000个精子,结果：杂交总有效率为98％,在B组男性不育XY和18号的二体频率为0．30％和0．30％,与对照组相比（0．15％,0％）无显差别,XY和18号失体的精子为0．15％和0％,与对照组（0％,0．15％）也无显差别,而A组的XY和18号染色体二体率为1．13％和0．96％,失体为1．13％和1．6％,与前两组相比,差异有显性,估算总的非整倍体率：A组为42．44％,B组为6．05％,对照组为2．59％,前与后两组相比,差异有显性,结论：不育患精子的染色体具有显的非整倍体性。     

白介素10和白介素17基因启动子区多态性位点与儿童哮喘 的相关性研究

目的:探讨IL-10基因启动子区-627A/C和IL-17基因启动子-152A/G位点多态性与儿童哮喘发生的相关性。方法:采用聚合酶链反应-限制性片段长度多态性分析(PCR-PFLP)方法检测186名哮喘儿童、198名健康儿童各个多态性位点的基因型,采用SPSS13.0进行统计学分析。结果:IL-17基因-152A/G位点的基因型及等位基因频率分布在哮喘组与正常对照组均存在显著性差异(p<0.05),哮喘组-152A/G位点等位基因A频率显著高于正常对照组(x2=6.077,p=0.014,OR=1.430,95%CI=1.076-1.902)。结论:IL-17基因-152A/G位点可能与儿童哮喘的发病存在关系,其中A等位基因可能是易感基因,携带A的个体可能更易患有哮喘。     

裕固族皮纹学初步研究

本文研究了甘肃裕固族青少年儿童346人的皮纹学特征,报道了斗、箕、弓各型指纹的频率,指纹指数,总指嵴数,a-b嵴数,通关手出现率,掌纹真实花样等8项皮纹学参数和主线止区的分布比例。并作了性别、手别、族别及人种间的比较。结果表明,裕固族既具有蒙古人种的皮纹特征,也有一些白种人的皮纹特点,这可能暗示着裕固族有白种人的血统。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.