"518"油桃主要害虫与其捕食性天敌的关系

为了科学施药,合理保护和利用天敌进行油桃园害虫的综合防治,用t检验法比较 “518”油桃季节间和年度间害虫及其天敌的种群差异,结果表明两年春-夏季之间小绿叶蝉、桃蚜、山楂叶螨和李肖叶甲4种害虫的t值分别为0.8049、1.1449、0.0700和0.9895,t值均小于t0.05（2.26）,差异均不显著;异色瓢虫、龟纹瓢虫、粽管巢蛛、八斑球腹蛛、三突花蟹蛛、草间小黑蛛、中华草蛉和黑带食蚜蝇8种天敌的t值分别为1.7014、0.9098、1.4304、0.6239、0.1545、0.0544、1.5579和0.8249,t值均小于t0.05（2.26）,差异均不显著。2009年春-夏与2009年秋季之间,桃蚜的t值为38.8150,粽管巢蛛的t值为9.1706,t>t0.01（3.355）,差异均极显著,异色瓢虫、三突花蟹蛛、黑带食蚜蝇的t值分别为2.3332,3.1497和2.4712,t>t0.05（2.306）,差异显著;2010年春-夏与2009年秋季之间,粽管巢蛛t值为7.0763,t>t0.01（3.355）,差异极显著,其余差异均不显著。采用灰色系统分析方法、生态位分析方法,对2009年及2010年春-夏季油桃园桃蚜、小绿叶蝉、山楂叶螨和李肖叶甲与其捕食性天敌在数量、时间和空间格局等方面关系进行分析,对每一种天敌对应 的关联度、生态位重叠指数和相似性比例等参数标准化后相加,按照参数标准化值之和大小排序得出,2009年春-夏季小绿叶蝉前三位的主要天敌是异色瓢虫、八斑球腹蛛和龟纹瓢虫;桃蚜的主要天敌是中华草蛉、粽管巢蛛和异色瓢虫;山楂叶螨的主要天敌是草间小黑蛛、三突花蟹蛛和龟纹瓢虫;李肖叶甲的主要天敌是草间小黑蛛、八斑球腹蛛和龟纹瓢虫。2010年春-夏季小绿叶蝉的主要天敌是龟纹瓢虫、八斑球腹蛛和草间小黑蛛;桃蚜的主要天敌是中华草蛉、黑带食蚜蝇和粽管巢蛛;山楂叶螨的主要天敌是八斑球腹蛛、草间小黑蛛和龟纹瓢虫;李肖叶甲的主要天敌是草间小黑蛛、八斑球腹蛛和异色瓢虫。2009年秋季小绿叶蝉的主要天敌是龟纹瓢虫、草间小黑蛛和异色瓢虫;桃蚜的主要天敌是八斑球腹蛛、异色瓢虫和粽管巢蛛;山楂叶螨的主要天敌是三突花蟹蛛、龟纹瓢虫和八斑球腹蛛;李肖叶甲的主要天敌是草间小黑蛛、八斑球腹蛛和三突花蟹蛛。4种害虫种群聚集均数λ均大于2,其聚集是害虫本身原因造成的,2009年5月5日的天敌异色瓢虫、中华草蛉和6月18日的天敌草间小黑蛛 值均大于2,聚集是由于天敌本身原因所致。三个时间段其余天敌的 值均小于2,表明其聚集时由环境中某一因子影响所致。     

茶园4种半翅目主要害虫与其捕食性天敌的关系

为了科学施药,合理利用和保护天敌进行假眼小绿叶蝉、通草粉虱、黑刺粉虱和茶蚜的综合防治,用灰色系统分析方法,生态位分析方法和空间格局聚集强度指标分析方法对潜山县茶园4种半翅目主要害虫与其捕食性天敌在数量、时间、空间格局等方面进行分析,评判出害虫的主要天敌,与假眼小绿叶蝉数量上跟随关系密切的天敌是三突花蟹蛛、斑管巢蛛、鞍型花蟹蛛、茶色新圆蛛和八点球腹蛛;时间上是三突花蟹蛛、斑管巢蛛、鞍型花蟹蛛、八点球腹蛛和茶色新圆蛛;空间上是三突花蟹蛛、斑管巢蛛、草间小黑蛛、八点球腹蛛和茶色新圆蛛;把与假眼小绿叶蝉在数、时、空方面参数大小的序号积加,和最小的即是第一位天敌,其综合排序得出假眼小绿叶蝉的主要捕食性天敌依次是三突花蟹蛛、斑管巢蛛、八点球腹蛛、茶色新圆蛛和鞍型花蟹蛛。与通草粉虱数量上跟随关系密切的天敌是锥腹肖蛸、日本球腹蛛、草间小黑蛛、龟纹瓢虫和八点球腹蛛;时间上是龟纹瓢虫、锥腹肖蛸、草间小黑蛛、八点球腹蛛和日本球腹蛛;空间上是八点球腹蛛、草间小黑蛛、龟纹瓢虫、异色瓢虫和三突花蟹蛛;把与通草粉虱在数、时、空方面参数大小的序号积加,综合排序得出通草粉虱的是龟纹瓢虫、草间小黑蛛、八点球腹蛛、锥腹肖蛸和异色瓢虫。与黑刺粉虱数量上跟随关系密切的天敌是草间小黑蛛、锥腹肖蛸、八点球腹蛛、异色瓢虫和鞍型花蟹蛛;时间上是草间小黑蛛、八点球腹蛛、锥腹肖蛸、异色瓢虫和斑管巢蛛;空间上是八点球腹蛛、草间小黑蛛、异色瓢虫、锥腹肖蛸和龟纹瓢虫;把与黑刺粉虱在数、时、空方面参数大小的序号积加,综合排序得出黑刺粉虱的是八点球腹蛛、草间小黑蛛、锥腹肖蛸、异色瓢虫和斑管巢蛛。与茶蚜数量上跟随关系密切的天敌是异色瓢虫、日本球腹蛛、草间小黑蛛、鞍型花蟹蛛和八点球腹蛛;时间上是日本球腹蛛、草间小黑蛛、鞍型花蟹蛛和茶色新圆蛛;空间上是异色瓢虫、草间小黑蛛、、八点球腹蛛、茶色新圆蛛和鞍型花蟹蛛;把与茶蚜在数、时、空方面参数大小的序号积加,综合排序得出茶蚜的是异色瓢虫、日本球腹蛛、草间小黑蛛、鞍型花蟹蛛和茶色新圆蛛。4种害虫种群聚集均数λ均大于2,其聚集是害虫本身原因造成的,天敌的种群聚集均数λ均小于2,其聚集是环境因子所致。     

三种蔷薇科果树小绿叶蝉及捕食性天敌种群动态的比较

对3种果树小绿叶蝉及其天敌数量进行t检验得出,樱桃园与梨园之间,小绿叶蝉、异色瓢虫、锥腹肖蛸差异显著、三突花蟹蛛差异极显著,均是樱桃园多于梨园;樱桃园与杏园之间,三突花蟹蛛、草间小黑蛛差异显著,异色瓢虫、锥腹肖蛸差异基本显著,均是樱桃园多于杏园,小绿叶蝉差异基本显著,杏园多于樱桃园;梨园与杏园之间,草间小黑蛛和小绿叶蝉均差异显著,杏园多于梨园。采用灰色系统分析方法研究樱桃小绿叶蝉数量与其天敌个体数量关联度,结果是,关联度较高的天敌依次是锥腹肖蛸、草间小黑蛛、八斑球腹蛛和中华草蛉,与理想优势种天敌数量关联度较高的天敌依次是三突花蟹蛛、异色瓢虫、草间小黑蛛和中华草蛉;利用生态位分析方法分析的结果是,与小绿叶蝉时间生态位和空间生态位重叠指数较大的天敌依次是异色瓢虫、草间小黑蛛、三突花蟹蛛和锥腹肖蛸;与小绿叶蝉两种生态位相似性比例指数较大的天敌依次是异色瓢虫、三突花蟹蛛、草间小黑蛛和锥腹肖蛸。综合排序分析,前4位的天敌依次是异色瓢虫、草间小黑蛛、三突花蟹蛛和锥腹肖蛸。     

棉蚜及其捕食性天敌时空生态位研究

研究结果表明,棉蚜的时间生态位宽度较小而空间生态位宽度较大,说明棉蚜种群的发生有高峰期明显和全株为害的特点．棉蚜的时动空间生态位宽度与种群密度无相关性,说明棉蚜的为害较复杂．捕食性天敌中草间小黑蛛和龟纹瓢虫的时空生态位宽度较大,说明它们发生期较长、分布范围较广．八斑球腹蛛和龟纹瓢虫与棉蚜的时间生态位重叠度较大,八斑球腹蛛和草间小黑蛛与棉蚜的空间生态位重量度较大．与棉蚜的时间×空间生态位重叠度,八斑球腹蛛＞龟纹瓢虫＞草间小黑蛛＞其它天敌合计＞三色长蝽＞三突花蛛＞小花蝽,这一结果同棉蚜与捕食性天敌数量的关联度大小排序相一致,表明捕食性天敌与棉蚜的时间同步和空间同线性与天敌的捕食作用密切相关．     

不同海拔茶园害虫、天敌种群及其群落结构差异

为了明确不同海拔茶园之间害虫、天敌种群及其群落结构的差异,为茶园害虫综合防治提供科学依据,采用平行跳跃抽样调查方法分别对海拔698.6m,270m和46m的高、中、低海拔茶园进行调查,并进行方差分析,进而用新复极差法进行多重比较,结果表明,以样方为单位3种海拔中差异极显著的害虫是假眼小绿叶蝉、柑桔粉虱、茶短须螨、茶黄蓟马、琥珀广翅蜡蝉和绿螽蟖;差异极显著的天敌是八点球腹蛛、锥腹肖蛸、日本球服蛛和茶色新圆蛛。数量居于3种海拔第一位的物种：高海拔茶园主要害虫为假眼小绿叶蝉、柑橘粉虱、茶短须螨和绿螽蟖,天敌为锥腹肖蛸蛛和八点球腹蛛;中海拔茶园害虫为茶黄蓟马和红蜡蚧,天敌为粽管巢蛛;低海拔茶园天敌为茶色新园蛛和日本球腹蛛。高海拔的主要害虫还有茶黄蓟马和琥珀广翅蜡蝉,主要天敌还有草间小黑蛛、茶色新圆蛛和日本球腹蛛;中海拔的主要害虫还有假眼小绿叶蝉和茶短须螨,主要天敌还有八点球腹蛛、草间小黑蛛、锥腹肖蛸、拟环纹狼蛛、三突花蟹蛛;低海拔的主要害虫有假眼小绿叶蝉、茶黄蓟马、柑桔粉虱和红蜡蚧,主要天敌还有八点球腹蛛、锥腹肖蛸、草间小黑蛛、粽管巢蛛和异色瓢虫。3种海拔茶园之间节肢动物群落多样性指数、个体数和均匀度差异均极显著,物种数差异不显著,3种海拔茶园之间植食性昆虫亚群落的多样性和物种数差异均不显著,个体数和均匀度差异均极显著。3种海拔茶园之间天敌亚群落的多样性指数、物种数和个体数差异均极显著。总群落多样性指数和群落相对稳定性值均是低海拔茶园最大。     

雨花露水蜜桃主要害虫与其捕食性天敌的关系

为了科学施药,合理保护和利用天敌进行水蜜桃园害虫的综合防治,用t检验法比较"雨花露"水蜜桃季节间和年度间害虫及其天敌的种群差异,结果表明两年春夏季之间小绿叶蝉、桃蚜、山楂叶螨、李肖叶甲和梨网蝽5种害虫的差异均不显著;异色瓢虫的t值为2.39,t>t_0.05(2.16),差异显著,其余天敌差异均不显著。2010年春夏与秋季之间,小绿叶蝉、梨网蝽、三突花蟹蛛和异色瓢虫的t值为5.63、76.38、3.28和4.82,t>t_0.01(3.01),差异极显著,其余差异不显著。采用灰色关联分析方法、生态位分析方法,对2010年及2011年春夏季水蜜桃园桃蚜、小绿叶蝉、山楂叶螨、梨网蝽和李肖叶甲与其捕食性天敌在数量、时间和空间格局等方面关系进行分析,对每一天敌对应的关联度、生态位重叠指数和相似性比例等参数标准化后相加,按照参数标准化值之和大小排序得出,2010年春夏季小绿叶蝉前三位的主要天敌是三突花蟹蛛、中华草蛉和八斑球腹蛛;山楂叶螨的主要天敌是粽管巢蛛、八斑球腹蛛和草间小黑蛛。2011年春夏季桃蚜的主要天敌是黑带食蚜蝇、异色瓢虫和三突花蟹蛛;小绿叶蝉的主要天敌是草间小黑蛛、龟纹瓢虫和三突花蟹蛛;梨网蝽的主要天敌是三突花蟹蛛、龟纹瓢虫和草间小黑蛛;山楂叶螨的主要天敌是粽管巢蛛、八斑球腹蛛和草间小黑蛛;李肖叶甲的主要天敌是草间小黑蛛、三突花蟹蛛和八斑球腹蛛。2010年秋冬季小绿叶蝉的主要天敌是中华草蛉、草间小黑蛛和三突花蟹蛛;梨网蝽的主要天敌是八斑球腹蛛、草间小黑蛛和三突花蟹蛛。林间,2010年小绿叶蝉、梨网蝽和山楂叶螨及2011年春夏季桃蚜、李肖叶甲、梨网蝽和山楂叶螨种群聚集均数λ均大于2,其聚集是害虫本身原因造成的。在桃树树冠四个方位间,2010年春夏季山楂叶螨,秋冬季小绿叶蝉和梨网蝽及2011年春夏季的李肖叶甲的λ值均大于2,其聚集是害虫本身原因造成的,其余害虫和天敌λ值均小于2,表明其聚集时由环境中某一因子影响所致。     

不同年份油桃园三种主要害虫与其天敌的关系

为了科学施药,合理保护和利用自然天敌进行油桃害虫的综合防治,用灰色系统分析方法、生态位分析方法和空间格局聚集强度指数分析方法,对2008年及2009年春-夏季油桃园桃蚜、小绿叶蝉和山楂叶螨与其主要捕食性天敌在数量、时间和空间格局等方面关系进行分析,两年春-夏季综合排序的结果是,桃蚜主要捕食性天敌依次是黑带食蚜蝇、异色瓢虫、三突花蟹蛛;小绿叶蝉主要捕食性天敌依次为三突花蟹蛛、锥腹肖蛸和草间小黑蛛;山楂叶螨主要捕食性天敌依次为草间小黑蛛、八斑球腹蛛和三突花蟹蛛。2008年秋季桃蚜的主要天敌依次是八斑球腹蛛、中华草蛉和锥腹肖蛸;小绿叶蝉的主要天敌依次是草间小黑蛛、锥腹肖蛸和黑带食蚜蝇;山楂叶螨的主要天敌依次为八斑球腹蛛、异色瓢虫和中华草蛉。两年春-夏季之间3种害虫及天敌数量差异均不显著。     

中稻田三种飞虱的捕食性天敌优势种及农药对天敌的影响

对两优0923非防治的中稻田白背飞虱、灰飞虱和褐飞虱与其天敌间的关系,采用灰色关联度法、生态位分析方法,对盆拍法调查的3种飞虱与其捕食性天敌在数量、时间和空间三方面关系进行分析,对每一种天敌对应的关联度、生态位重叠指数和相似性比例等参数标准化后的密切指数相加,按照密切指数值之和大小排序,评判3种飞虱捕食性天敌优势种.并用同样方法分析常规防治田农药对飞虱捕食性天敌的影响,以期为合理施药,科学保护和利用天敌优势种提供科学依据,其结果是,非防治田白背飞虱前三位天敌是条纹蝇虎、草间小黑蛛和锥腹肖蛸;灰飞虱的是八斑球腹蛛、茶色新园蛛和锥腹肖蛸;褐飞虱的是纵条蝇狮、四点亮腹蛛和黑肩绿盲蝽.防治田白背飞虱前三位天敌是条纹蝇虎、草间小黑蛛和锥腹肖蛸;灰飞虱的是拟水狼蛛、四点亮腹蛛和草间小黑蛛;褐飞虱的是黑肩绿盲蝽、拟水狼蛛和四点亮腹蛛.盆拍法的防治田和非防治田之间3种飞虱前三位的相同天敌,白背飞虱完全相同,灰飞虱没有相同天敌,褐飞虱的是黑肩绿盲蝽和四点亮腹蛛.飞虱与天敌在时间和数量关系上,扫网法的防治田和非防治田之间3种飞虱前三位的相同天敌,白背飞虱的是锥腹肖蛸和四点亮腹蛛,灰飞虱完全相同,褐飞虱的是纵条蝇狮和条纹影虎.两种稻田的差异主要是农药杀伤了飞虱,使飞虱数量减少,并对天敌有一定杀伤力,进而影响到天敌的发生规律.非防治田的盆拍法和扫网法之间,3种飞虱前三位相同的天敌,白背飞虱的是锥腹肖蛸,灰飞虱的也是锥腹肖蛸,褐飞虱的是纵条蝇狮.防治田两调查方法结果之间,白背飞虱的是锥腹肖蛸,灰飞虱和褐飞虱前3位天敌中没有相同天敌,其差异主要是两法调查稻株的部位不同所致.     

影响棉蚜种群数量的优势种天敌的灰色系统分析

对1983、1984、1986、1988、1994和1995年6年棉花铃期以前棉蚜种群数量与其天敌种群数量以及各种天敌的捕食量,利用灰色系统理论和方法,研究天敌的日捕食总量和棉蚜种群数量的追随关系,并分析各种天敌与理想优势种天敌之间的联系程度,结果表明,龟纹瓢虫是棉蚜的主要天敌,其次是大草蛉,第三是八斑球腹蛛和草间小黑蛛.     

石榴园棉蚜及其天敌之间的关系

采用灰色系统分析方法,分析了对主要害虫棉蚜及其天敌日捕食总量之间关联度,结果表明,棉蚜群落中与其关联度较高的天敌种类依次为肖蛸蛛(0．8607)、中华草蛉(0．8058)、八斑球腹蛛(0．7989)、异色瓢虫(0．7881)和大草蛉(0．7758),与理想天敌日捕食总量间关联度较高的天敌种类为草间小黑蛛(0.8975)、智利小绥螨(0．8132)、龟纹瓢虫(0.7806)和大草蛉(0．7669);与天敌数量间关联度较高的天敌依次为草间小黑蛛(0．8482)、中华草蛉(0．7533)、肖蛸蛛(0．7532)、八斑球腹蛛(0．7411)和大草蛉(0．7116),与理想天敌数量间关联度较高的为草间小黑蛛(0．8461)、智利小绥螨(0．7325)、龟纹瓢虫(0．6983)、大草蛉(0．6815)和中华草蛉(0．6757)．棉蚜垂直生态位和水平生态位的重迭值均>0．9567的天敌有草间小黑蛛、大草蛉和肖蛸蛛;时间生态位重迭值>0．4020的天敌有草间小黑蛛、大草蛉和肖蛸蛛,表明草间小黑蛛、大草蛉、肖蛸蛛等为棉蚜主要天敌优势种。     

Relative and combined effects of ethanol and protein deficiency on strontium and barium bone content and fecal and urinary excretion

To elucidate accumulation of minerals in human iliac arteries with aging, the content of minerals was analyzed by inductively coupled plasma atomic emission spectrometry. Bilateral common, internal, and external iliac arteries of 16 men and 8 women, ranging ages from 65 to 93 yr, were examined. It was found that an extremely high accumulation of calcium and phosphorus occurred in the common iliac artery at old age, being higher than that of the internal and external iliac arteries. It should be noted that the accumulation of calcium and phosphorus is the highest in the common iliac artery among the human arteries examined to date. Regarding sexual differences, the content of calcium and phosphorus in the common and internal iliac arteries was higher in women than in men, whereas their content in the external iliac artery was lower in women than in men.     

New amino-dithiaphospholanes and phosphoramidodithioites and their rhodium and iridium complexes

New sulfur derivatives of phosphoramidite ligands were synthesized and the impact of the sulfur unit on the spectroscopic properties of their rhodium and iridium complexes was investigated. The new ligands Bn₂NPSCH₂CH₂S^a(P-S^a) (Bn = benzyl, 4), Bn₂NPSCHCHS^a(CH₂)₃C^aH₂(P-S^a)(C^a-S^a) (6) and Bn₂NP(4-XC₆H₄OMe)₂ (X = S, 7a; X = O, 7b) were converted to the rhodium and iridium complexes trans-[Rh(CO)Cl(L)₂] (L = 4, 6, 7), [RhCl(COD)(L)] (L = 4, 6, 7), [IrCl(COD)(7a)] and [IrCl₂Cp∗(6)]. For comparison, some phosphoramidite complexes of these formulations also were synthesized. The new metal complexes were spectroscopically analyzed. For the carbonyl complexes, the ν_CO IR stretching frequencies were lower than for the corresponding phosphite and phosphoramidite ligands. The ¹J_PRh coupling constants for the rhodium complexes with the new ligands were also smaller than for the respective phosphoramidite and phosphite complexes. Finally, the ¹J_PSe coupling constants of the selenides of the new ligands were lower than those of the phosphoramidite ligands but higher than for PPh₃. The spectroscopic data reveal that the new thio ligands 4, 6 and 7a are more electron donating than phosphites and phosphoramidites but less electron donating than PPh₃.     

Astrocytes and Synaptosomes Transport and Metabolize Lactate and Acetate Differently

Astrocytes transport the monocarboxylate acetate, but synaptosomes do not. The reason for this is unknown, because both preparations express monocarboxylate transporters (MCT). The transport and metabolism of lactate, another monocarboxylate, was examined in these two preparations, and the results were compared to those for acetate. Lactate transport is more rapid in astrocytes than in synaptosomes, but of lower affinity (Kms of 17 and 4 mM, respectively). Lactate (0.2 mM) is metabolized to CO2 more rapidly in synaptosomes than in astrocytes (rates of 0.37 and 0.07 nmol x mg protein(-1) x min(-1), respectively). The reason for this is unclear, but cellular differences in lactate dehydrogenase isotype expression may be involved. Acetate is metabolized to CO2 more rapidly in astrocytes than in synaptosomes (rates of 0.43 and 0.02 nmol x mg protein(-1) x min(-1), respectively). This is likely due to cellular differences in the expression of monocarboxylate transporter subtypes.     

白蚁与动物及微生物的共生类型及其机制

综述了白蚁螱客的主要种类、共生关系及相关机制的研究进展。白蚁螱客中,已报道的动物种类达170种。在与动物的共生关系中存在偏利共生（宾主共栖和异种共栖）、互利共生和无关共生三种;在与微生物的共生关系中,存在与内生菌（原生动物、细菌、真菌和放线菌）和外生菌（蚁巢伞菌等）间的互利关系。指出了白蚁与螱客研究中存在的问题,给出了解决方案,并提出了今后可能的研究热点或方向,为白蚁的综合利用（如纤维素酶）及今后研究物种间的协同进化提供了基础资料。     

Rapporteur report: Basics and technology and metrology and standards

The first and second sessions of the Workshop focussed on the basics of ultrasound and infrasound, their applications in both industry and medicine, and metrology and protection standards for ultrasound applications.     

Prostaglandin and thromboxane production by human and guinea-pig macrophages and leucocytes

The ability of partially purified human and guinea-pig haematogenous cell populations, when cultured in vitro, to metabolise arachidonic acid (AA) has been studied. Supernatants from 24 hour cell culture have been subjected to analysis for products of AA metabolism by gas chromatography with electron-capture detection.The cell types studied were human peripheral blood monocytes (both glass adherent and non-adherent), neutrophils, eosinophils and leukemic leucocytes; thoracic duct lymphocytes and lung alveolar macrophages. From the guinea-pig, induced and non-induced macrophage or neutrophil enriched peritoneal exudate populations, lymph node cells, peritoneal eosinophils and peripheral blood platelets were examined. Supernatants were assayed for the presence of PGE₂, PGD₂, PGF_2α, TXB₂ and 6-keto-PGF_1α. In all types studied PGE₂ and TXB₂ were the major products formed. The identification of PGE₂ and TXB₂ was confirmed by GC/MS with multiple ion monitoring.The results have been compared with other reports and their possible significance discussed in relation to the proposed role of prostaglandins as mediators and modulators in immunopathology.     

Cytoskeleton and mitochondrial morphology and function

It has been well established that the cytoskeleton is an essential modulator of cell morphology and motility, intracytoplasmic transport and mitosis, however cytoskeletal linkage to the organelles has not been unequivocally demonstrated. Indeed, cytoskeleton appears to be essential in determining and modulating gene phenotype as a function of cellular environment. According to recent studies, the organization of the cytoskeleton network together with associated protein(s) could be essential in regulating mitochondrial function and particularly the permeability of the mitochondrial outer membrane to ADP. The aim of this chapter is to summarize the main properties of the cytoskeletal environment of mitochondria and the possible role(s) of this network in mitochondrial function in myocytes.     

Cross-reactivity between storage and dust mites and between mites and shrimp

Many patients have sensitivities to multiple species of storage and house dust mites. It is not clear if this is because patients have multiple sensitivities to species-specific mite allergens or if these mites share many cross-reacting allergens. Our objective was to further define the cross-allergenicity between several species of storage and house dust mites using crossed-immunoelectrophoresis (CIE), crossed-radioimmunoelectrophoresis (CRIE), immunoblotting, and ELISA. CIE and CRIE reactions revealed that storage mites shared two cross-antigenic molecules and one of these bound IgE in a serum pool from mite allergic patients. Antibody in anti-sera built to each species of mite recognized many SDS–PAGE resolved proteins of other mite species and this suggested the potential for other cross-reactive allergens. Among patient sera, IgE bound to many different proteins but few had IgE that bound to a protein with common molecular weights across the mite species and this suggested mostly species-specific allergens. Antiserum built to each mite species precipitated one protein in shrimp extracts that bound anti-Der p 10 (tropomyosin) and IgE in the serum pool. Anti-Der p 10 showed strong binding to shrimp tropomyosin but very little to any of the mite proteins. ELISA showed the mite extracts contained very little tropomyosin. The storage and dust mites investigated contain mostly species-specific allergens and very small amounts of the pan-allergen tropomyosin compared to shrimp and snail.