江西官山自然保护区四种雉类的生境选择差异

2009年10月—2010年11月在江西官山国家级自然保护区对白颈长尾雉、白鹇、勺鸡和灰胸竹鸡4种雉类的生境选择进行了研究:共调查了6条样线上的388个样方,选取了与这四种雉类栖息地相关的17个生态因子进行观测和测量。结果显示,4种雉类均偏好阔叶林和针阔混交林、阳坡或半阴半阳坡的生境。Kruskal-Wallistest检验表明,4种雉类在海拔、坡度、乔木盖度、灌木盖度、灌木数量、灌木高度、草本盖度、草本种类、草本数量、落叶层盖度和水源距离11个生态因子上存在极显著差异(P<0.01),乔木种类存在显著差异(P<0.05),其余生态因子无显著差异(P>0.05)。典则判别函数图显示,4种雉类在生境选择上存在一定程度的重叠,又有比较明显的差异。逐步判别表明在区分4种雉类生境选择差异方面有一系列的生态因子发挥作用,依照贡献值的大小依次为海拔、草本数量、灌木高度、水源距离、灌木盖度、乔木盖度、坡度、落叶层盖度、灌木数量、草本盖度。由这10个变量构成的方程对4种雉类生境选择差异的正确区分率为74.7%。     

广西金钟山片段化生境中黑颈长尾雉夏季栖息地选择

2011和2012年夏季,采用无线电跟踪技术跟踪了8只野生黑颈长尾雉(6♂,2♀),野外共测量了204个10 m×10 m样方的20个生态因子,其中利用样方111个,未利用样方93个。采用独立样本的t检验或非参数Mann-Whitney U定量分析表明:黑颈长尾雉夏季栖息地利用和未利用样方的生态因子坡位、与林缘距离、草本盖度、灌木高度、草本密度和乔木胸径差异不显著(P0.05),其余的生态因子在利用和未利用样方存在显著差异(P0.05)。主成分分析显示:夏季特征值大于1的主分量共有5个,其累计贡献率达到64.23%,第1主成分为食物因素,是影响黑颈长尾雉夏季栖息地选择的主要因素;其余主成分分别为安全因素、干扰因素、地理因素和水源因素。不同生境斑块类型中食物资源的可用性影响夏季黑颈长尾雉栖息地选择。     

云南大中山黑颈长尾雉栖息地选择周年变化

采用比较利用和可利用栖息地的方法,分别对云南大中山黑颈长尾雉秋冬季、春季和夏季栖息地展开调查。检验和Bonferroni置信区间分析结果表明,黑颈长尾雉不同季节均偏向选择常绿阔叶林,偏向选择或回避选择的乔木、灌木和草本的种类不尽相同。利用和可利用样方差异性检验显示,不同季节对地形因素的选择性不明显,仅夏季距水源距离一个因子差异显著;植被因子中,春季差异显著的仅有乔木层盖度因子,夏季有乔木密度、乔木层盖度、落叶层盖度和草本层盖度等4个因子,秋冬季有乔木层盖度、草本层盖度和藤本密度等3个因子。主成分分析表明,不同季节利用样方负荷绝对值较大的因子在各主成分中的序位不尽相同,即栖息地选择的主要生态因子随季节发生变化。单因素方差分析和逐步判别分析表明,秋冬季与春季栖息地特征较接近,而与夏季差别较大。χ2     

再引入黑颈长尾雉夏季栖息地选择

2008年6～7月,在广西岑王老山国家级自然保护区采用样带法对再引入黑颈长尾雉(Syrmaticus humiae)的栖息地选择进行了研究。共测定了7条样带上的22个栖息地样方和17个对照样方的24个生态因子。结果表明,再引入黑颈长尾雉的夏季栖息地偏向于选择海拔低、坡位低、乔木较低和灌木较高、落叶盖度较大、灌木层盖度大、草本高且盖度大的阔叶林、针阔混交林或者针叶林;逐步判别分析的结果显示坡位、草本层盖度是重要的生态因子,由这两个变量构成的方程在对栖息地和对照区进行区分时,正判率可以达到77.3%,这种选择与黑颈长尾雉的隐蔽条件和食物密切相关。     

广西黑颈长尾雉对夜宿地的选择

2003年12月、2004年5月和12月、2005年4月,通过野外调查及设置样方的方法对广西金钟山鸟类保护区黑颈长尾雉的夜宿地选择进行了研究。共记录到12个夜宿地,以夜宿树为中心做一个10m×10m和5个1m×1m的样方,记录夜宿树特征(种类、胸径、栖枝高度、栖枝直径、栖枝上方盖度),测量每个样方的11个生境因子(海拔、林型、坡向、坡度、乔木层盖度、灌木层盖度、草本层盖度、距水源距离、距林缘距离、距道路距离和乔木密度);并随机设10个对照点,测量相同的生境因子。结果表明,黑颈长尾雉夜宿地主要位于阔叶林中,一般,夜间在乔木上休息,一树栖息一只,也有2—3只同宿一树。黑颈长尾雉选择有一定坡度、林下灌木和草本较少以及在森林内部远离林缘并且乔木密度较大的地方作为夜宿地,其夜栖树主要为生境内的中等大小的阔叶树,栖枝直径约为2·5—4cm,栖处高度为2—5m,黑颈长尾雉夜宿地的选择与距水源距离的远近无关。影响黑颈长尾雉夜宿地选择的因素依次为:位置及林下植被、栖枝、天气。影响黑颈长尾雉夜宿地选择的最终原因可能是安全、舒适和栖息地转换的方便程度。     

广西黑颈长尾雉对夜宿地的选择

2003年12月、2004年5月和12月、2005年4月，通过野外调查及设置样方的方法对广西金钟山鸟类保护区黑颈长尾雉的夜宿地选择进行了研究。共记录到12个夜宿地，以夜宿树为中心做一个10 m×10 m和5个1 m×1 m的样方,记录夜宿树特征(种类、胸径、栖枝高度、栖枝直径、栖枝上方盖度)，测量每个样方的11个生境因子(海拔、林型、坡向、坡度、乔木层盖度、灌木层盖度、草本层盖度、距水源距离、距林缘距离、距道路距离和乔木密度)；并随机设10个对照点，测量相同的生境因子。结果表明，黑颈长尾雉夜宿地主要位于阔叶林中，一般，夜间在乔木上休息，一树栖息一只，也有2—3只同宿一树。黑颈长尾雉选择有一定坡度、林下灌木和草本较少以及在森林内部远离林缘并且乔木密度较大的地方作为夜宿地，其夜栖树主要为生境内的中等大小的阔叶树，栖枝直径约为2.5—4 cm，栖处高度为2—5 m，黑颈长尾雉夜宿地的选择与距水源距离的远近无关。影响黑颈长尾雉夜宿地选择的因素依次为：位置及林下植被、栖枝、天气。影响黑颈长尾雉夜宿地选择的最终原因可能是安全、舒适和栖息地转换的方便程度。     

再引入黑颈长尾雉育雏行为和育雏地选择

2004～2005年在广西岑王老山自然保护区对再引进黑颈长尾雉的育雏行为和育雏地选择进行了研究.结果表明:雏鸟活动能力随时间明显增强,1～3日活动面积在300 m2以内.育雏初期为地栖,后期开始为树栖.育雏地有3种类型:人工西南桦幼林、针叶林、针阔混交林.3种生境的乔木盖度分别是75%、90%、85%;草本盖度分别是80%、60%、65%.Mann-Whitney U分析表明草本层盖度,水源距离,林缘距离,林间小路距离是影响黑颈长尾雉育雏地选择的主要因子.     

白颈长尾雉春季扩散活动的影响因子

2002年10月-2003年6月和2003年11月-2004年6月,采用无线电遥测、GPS技术、SAW模型和样方法,对浙江省古田山自然保护区白颈长尾雉的扩散过程及其影响因子进行了研究。结果表明：①白颈长尾雉在春季均存在持续约为16—23d的扩散活动,其扩散直线距离在1．5～2．1km。②扩散过程中白颈长尾雉对其运动路线存在选择性,在其扩散进入和未进入区域之间,草本盖度、乔木种数、灌木种数、灌木枝下高、灌木数量和草本数量存在极显著差异;距水源距离、坡度、乔木数量、乔木枝下高和草本种数存在显著差异。③灌木种数、灌木枝下高、坡度和灌木数量等与障碍有关的因子是影响白颈长尾雉扩散活动的重要因子。     

四川卧龙国家级自然保护区多空间尺度下绿尾虹雉的生境选择

动物的生境选择具有空间尺度依赖性, 在不同空间尺度上影响生境选择的环境因素有所不同。研究不同空间尺度下动物生境选择的关键影响因子及其季节性变化, 对于全面了解物种的生境资源需求和开展生境保护具有重要意义。绿尾虹雉(Lophophorus lhuysii)是中国特有的高山雉类, 国家一级重点保护野生动物, 具有极高的保护价值。然而, 目前尚未对其不同尺度和时期的生境选择进行过探究。本研究于2019年10月至2020年10月, 在四川卧龙国家级自然保护区的羊角湾、魏家沟和文扎都3个区域共布设15条样线、303个样方, 并结合红外相机监测(176个红外相机位点), 对保护区内绿尾虹雉种群的生境利用状况进行了调查, 使用主成分分析和逻辑斯蒂回归模型分别从景观和微生境两个尺度对繁殖期(3‒8月)和非繁殖期(9月至翌年2月)的生境选择模式进行了分析。结果显示, 在景观尺度上, 在繁殖期和非繁殖期都显著偏好海拔较高(3,700‒ 4,300 m)、坡度较小(27°‒33°)、靠近阳坡、草甸和流石滩比例较高而森林和灌丛比例较低的生境。在微生境尺度上, 绿尾虹雉在繁殖期显著偏好岩石盖度较高的生境; 而非繁殖期则显著偏好草本盖度较高、灌木盖度和落叶盖度较低的生境。研究表明, 绿尾虹雉在景观和微生境尺度上均对生境有明显的选择性, 并且其微生境选择还存在季节性变化, 反映了该物种在生活史不同阶段具有不同的资源需求。本研究丰富了绿尾虹雉的基础生态学信息, 为卧龙及其他自然保护区绿尾虹雉的生境管理和种群保护工作提供了参考。     

哀牢山国家级自然保护区南华片三种雉类春季取食地利用比较

探讨亲缘关系密切、营养关系相似的物种间的共存机制,是群落生态学的研究热点之一。研究结果有助于了解同域物种的资源利用特点和分化方式,对于地区生物多样性的保育工作亦具有指导意义。黑颈长尾雉(Syrmaticushumiae)、白鹇(Lophuranycthemera)和红喉山鹧鸪(Arborophilarufogularis)为哀牢山国家自然保护区南华片区同域分布的3种雉类,它们亲缘关系较近,食性相似。比较三者春季取食地18个生态因子生态位宽度和重叠指数,结果显示,黑颈长尾雉广适性(生态位宽度值>0·8)的取食地因子最少(1个),专一性(生态位宽度<0·8)因子最多(4个);白鹇居中(3个和2个);红喉山鹧鸪与黑颈长尾雉恰好相反(6个和0个)。黑颈长尾雉与白鹇生态位重叠指数≥0·5的因子数(5个)多于黑颈长尾雉与红喉山鹧鸪的(2个)和白鹇与红喉山鹧鸪的(4个);灌木层盖度、草本层盖度和种子密度的生态位重叠指数在3种雉类间均较大。单因素方差分析结果表明,3种雉类在坡向、乔木密度、草本层盖度、种子密度和土壤动物等5个因子均无差异;在距空旷地距离、距道路距离和灌木层盖度等3个因子均有差异;而其余因子则是在不同的两个物种之间有差异。主成分分析结果显示,黑颈长尾雉取食地前7个主成分的累计信息量达85·6%,白鹇的前6个达86·0%,红喉山鹧鸪的前5个达79·0%。影响这三种雉类取食地选择的因子主要集中于前3个主成分。3种雉类间取食地因子差异状态呈镶嵌分布,意味着种间生态位呈分化状态。各物种优先选择的因子组合不同,各因子的重要程度亦不一。     

Vermicomposting in the management of pig-waste in Hong Kong

The treatment and disposal of pig-waste in Hong Kong has received much attention in recent years but, following any of the presently used treatment processes, solids remain to be further stabilized. Vermicomposting is a waste stabilization technique which converts waste into potentially recyclable materials such as worm protein and worm casts. The earthworm, Pheretima asiatica, can stabilize most of the solids arising from the treatment of pig-waste, including raw pig manure, suggesting that vermicomposting has a high potential as a unit process in the management of pig-waste in Hong Kong.S.H. Wong is with the Environmental Protection Department, Hong Kong; and D.A. Griffiths is with the Department of Botany, University of Hong Kong, Hong Kong.     

Removal of wastewater pathogen indicators in a constructed wetland in Leon, Spain

This paper studies the seasonal presence and removal of the pathogenous micro-organisms Escherichia coli, total coliforms (TC), Clostridium perfringens (Cp), faecal streptococci (FS), Giardia cysts, Cryptosporidium oocysts and helminth eggs, in a constructed wetland treatment system. The removal efficiency of this system with respect to the indicator micro-organisms achieved maximum values in spring and autumn at 99.9% for E. coli and TC, respectively, in winter at 97.0% for FS, in summer at 100% for Clostridium and throughout the year, also at 100%, in the case of Giardia cysts, Cryptosporidium oocysts and helminth eggs. In general, very low protozoan and helminth egg counts were found, and the system demonstrated efficient reduction of the wastewater indicator pathogens.     

Inbreeding in a french peasant population

Genealogical pedigrees were collected from 976 farming couples who lived in a canton of central France in the XIXth and XXth century. Several methods (genealogical, isonymic and probability of origin of genes) were used to appraise the evolution of the biological structure of this population, which has gone through two phases. The first phase from 1800 to the World War I, shows gene flows between the farming population of the canton and those of neighbouring rural districts which are linked to an increase in consanguineous marriages. The second phase, from 1920 onwards, shows important gene flow from distant areas. This trend implies a decrease in consanguineous marriages.     

Lactic acid bacteria in fermented foods in Thailand

Traditional fermented foods (fish, meat and vegetable products), produced by many different processes, are eaten in many parts of Thailand. Lactic acid bacteria are responsible for the souring and ripening of these foods. Homofermentative strains of Lactobacillus pentosus, L. plantarum and Pediococcus pentosaceus are dominant in foods with low salt concentrations whereas P. halophilus strains are present in foods containing high salt. Strains of Lactobacillus sake, other Lactobacillus spp., P. acidilactici and P. urinaeequi are frequently found. Heterofermentative strains of L. brevis, L. confusus, L. fermentum, L. vaccinostercus, other Lactobacillus spp., and of Leuconostoc spp. are distributed as minor bacteria and strains of Staphylococcus, Enterococcus and Halobacterium are occasionally isolated.S. Tanasupawat is with the Department of Microbiology, Faculty of Pharmaceutical Sciences. Chulalongkorn University, Bangkok 10330, Thailand; K. Komagata is with the Department of Agricultural Chemistry, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156, Japan.     

New species and new combinations in Peruvian Orchidaceae

seventeen new species and combinations are proposed in the generaChondrorhyncha, Cischweinfia, Cochlioda, Eloyella, Encyclia, Kefersteinia, Koellensteinia, Macroclinium, Rodriguezia, Solenidiopsis, andStenia. All new species are illustrated. A key is provided for 2-flowered species ofMacroclinium, PeruvianSigmatostalix, and PeruvianStenia. Solenidium (Solenidiopsis) peruvianum Schltr. is lectotypified.     

Phylogenetic relationships in theCampanulales based onrbcL sequences

Phylogenetic relationships within the angiosperm orderCampanulales were investigated by comparative sequencing of the chloroplast generbcL. CompleterbcL sequences were obtained for ten species in six families within the order. These data were analyzed along with previously publishedrbcL sequences from other taxa (for a total of 117 species) within the subclassAsteridae and outgroups, producing 32 equally parsimonious trees. A subset consisting of 44 of these taxa was then chosen and more rigorous analyses performed, resulting in four equally parsimonious trees. Results indicate that two major clades roughly corresponding to traditionally circumscribedAsterales andCampanulales exist as sister taxa. In particular, therbcL trees indicate thatSphenoclea is not a member ofCampanulales orAsterales, thatPentaphragma is more closely allied toAsterales thanCampanulales, that theCyphiaceae are not monophyletic, thatCampanulaceae andLobeliaceae are not sister taxa, and thatStylidiaceae are correctly placed withinCampanulales.     

Nectary structure and nectar characteristics in someBignoniaceae

The nectary structure and chemical nectar composition of 15 species belonging to 12 genera ofBignoniaceae are analyzed. All taxa bear a conspicuous nuptial nectary surrounding the ovary base. The secretory tissue is mostly supplied by phloem branches. The stomata are located in the middle and upper part of the nectary epidermis with an homogeneous distribution. The nuptial nectary is proportionally large in relation to the ovary (15–30%), disregarding the nectary volume. Most species have extranuptial nectaries in both inner and outer surfaces of the calyx. Both kinds of nectaries lack a vascular tissue that straightly supplies them. Nuptial nectar concentration (wt/wt) ranges from 19 to 68%. Sugars and amino acids are found in all species. Half of the species have hexose predominant nectars, the remaining sucrose predominant. Phenols are detected in only three species, whereas reducing acids exclusively inTecoma stans. Alkaloids and lipids were never detected. Extranuptial nectar chemical composition is analyzed in two species:Dolichandra cynanchoides andPodranea ricasoliana. Bees constitute the main flower visitors of the species studied whereas hummingbirds were seen visiting three species. A correlation analysis is performed with the data obtained. There are a few significant correlations which indicate a parallel increase of three parameters: the longer the flower length, the more voluminous the nectary and the higher stomata number, independently of the floral biotype. Phenograms are obtained using 24 floral characters including nectary and nectar data. The clusters obtained do not reflect taxonomic relationships but are useful in the understanding of animal-plant interactions when the flower biotype is considered.This paper is based on a chapter of a doctoral thesis presented at the University of Córdoba (Argentina).     

Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants

Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine River which had been polluted 3 years previously in 1986. Mercuric reductase and organomercurial lyase genes persist in the bacterial genome even after the disappearance of the pollutant but are absent in axenic amoebae. A total of 49 bacterial isolates showed DNA homologies with the³²P-labelled DNA probes and 15 free-living amoebae were selected due to their harboured symbiotic mercury-resistant bacteria.     

Mycoflora of Argentinian corn harvested in the main production area in 1990

Corn (Zea mays) is the main cereal produced in and exported from Argentina. The risk of contamination by mycotoxins is related to the mycoflora associated with the corn kernels. This paper reports on the identification of internal and external mycoflora of corn kernels harvested in the main production area in Argentina in 1990. A mycological survey was carried out on 178 corn samples, from five locations in that area and the isolation frequency and relative density of the prevalent fungal genera compared. GenusFusarium was the most prevalent component of the internal seedborne mycoflora in the five locations.Penicillium was prevalent in all locations, taking into account the frequency. However, this genus was predominant only in two locations, when the relative density was considered. The predominantFusarium wasF. moniliforme and the most frequently isolated species ofAlternaria, Aspergillus andPenicillium wereA. alternata, A. flavus andP. decumbens, respectively.Diplodia species were not isolated from any of the samples.