横斑锦蛇的形态学性状初探

横斑锦蛇Euprepiophis perlacea是中国西部特有种,《中国濒危动物红皮书》极危物种,研究成果相对匮乏。本研究采用样带法和样方法在横斑锦蛇分布区内采集到标本20号,测量其鳞片特征、体长、尾长等形态学性状,结合文献资料中已有的5号标本数据,进行统计分析。结果表明,鳞片特征是有效的分类依据,尤其背鳞(19-19-17行)、左上唇鳞数(7)和右上唇鳞数(7)在所有标本中无任何变化,是鉴定该物种的最重要特征。对尾下鳞、体长、尾长、头长、头宽、头高、眼间距共7个形态学性状数据进行方差分析,性别间的差异均无统计学意义,未发现蛇类中普遍存在的两性异形现象。横斑锦蛇体色靓丽、无毒、性情温和、行动迟缓、分布区狭窄、种群数量稀少,分布区内人口密度高,人为干扰严重,急需加强科学研究、法制宣传和科普教育,以保障该物种种群延续。     

黑眉镜蛇繁殖的初步观察

黑眉镜蛇Elaphe taeniura Cope俗名菜花蛇,在我国分布较广,是一种常见的无毒蛇。它捕食鼠类,它的皮可做乐器,胆可药用,肉可吃,是一种有经济价值的动物。我国有些地方,黑眉镜蛇的产量很大,在四川有些县一年能收活蛇几万条,其中黑眉镜蛇约占三分之一。因此,摸清黑眉锦蛇的活动规律和习性,合理捕捉利用,或者研究人工饲养繁殖,是有一定意义的。我们于1965年在动物园里对黑眉锦蛇的繁殖进行了观察,现把初步观察材料整理如下:     

几种游蛇的Cyt b基因片段序列分析及其演化关系

分别从蛇类药材和冷冻保存的新鲜蛇类肌肉标本中提取DNA,经PCR扩增出12种蛇共25个样品的Cyt b基因片段,并用银染测序的方法对DNA序列进行了分析.在此基础上用MEGA软件重建的系统发生树表明,研究的11种游蛇科蛇类可以分为3组:第一组为赤链蛇和水赤链游蛇,第二组为乌梢蛇和灰鼠蛇,第三组为锦蛇属的蛇,它们与第二组较近.锦蛇属是一高度分化的属,该组至少可分为两类,一类包括百花锦蛇和黑眉锦蛇;另一类包括玉斑锦蛇、棕黑锦蛇、红点锦蛇、王锦蛇和双斑锦蛇.后一类还可进一步分为3个亚组,玉斑锦蛇和棕黑锦蛇为第一亚组,红点锦蛇单独为第二亚组,王锦蛇和双斑锦蛇为第三亚组.本研究结果还表明,多年保存的陈旧药材标本可以用DNA序列分析的方法对其进行分子系统演化关系的研究.     

四川美姑大风顶自然保护区两栖爬行动物资源调查

20 0 2年 8月 1日～ 17日对四川美姑大风顶自然保护区的两栖爬行动物进行了调查。结合前人的工作 ,该保护区共有两栖爬行动物 2 2种 ,其中两栖动物 11种 ,隶属于 9属 7科 2目 ,爬行动物 11种 ,隶属于 9属 4科 2亚目 1目。区系特点为 :(1)物种组成较丰富 ;(2 )都属于东洋界物种 ,没有古北界的成分 ;(3)区系组成较简单 ,以喜马控雅 -横断山区型和南中国型为主 ;(4 )特有种所占比例高。 (5 )既古老又具年轻的特点。美姑县新纪录 6种 ,大凉疣螈为国家Ⅱ级重点保护野生动物 ,横斑锦蛇为四川省重点保护野生动物。美姑大风顶自然保护区两栖爬行动物物种多样性和生境多样性较高 ,对于生物多样性保护和研究横断山脉两栖爬行动物物种组成及区系特征具有一定意义。     

裴氏金线鱼的重新描述及其与近似种横斑金线鱼的形态学比较(英文)

裴氏金线鱼和横斑金线鱼是金线鱼属中 2 种重要的经济鱼类,广泛分布于我国的东南沿海及印度太平洋海域。由于这 2 种金线鱼外部形态十分相似,导致其分类鉴定非常困难。尽管过去有些学者曾经对它们分别进行过形态描述,但是目前文献中仍存在许多同物异名及鉴定错误,特别是在中国这 2 种鱼类的命名和鉴定始终未能形成统一的共识。本文对裴氏金线鱼和横斑金线鱼进行了形态学比较研究,发现裴氏金线鱼和横斑金线鱼在体型、体色、背鳍及鳃弓的形状等特征上存在明显差异。金线鱼属是我国重要的渔业种类,研究它们的形态分类学特征并进行准确的物种鉴定,对该属鱼类单一种类的资源评估和资源保护具有重要意义。     

小猫能的捕捉及饲养

小猫熊(Ailurus fulgens)在四川的分布为北起邛崃山脉,沿大雪山向东南到大凉山,在此范围内尤以宝兴、天全、石棉、峨边、康定等县为多。在成都附近的灌县、崇庆等县境内也有零星发现。各地群众根据它的形态、习性有着不同的俗名,如汶川地区猎人根据小猫熊有时在冬季要爬往向阳的山崖处晒太阳取暖的习惯,叫“山车娃”和“山门蹲”。康定地区以它腰部的颜色和尾上的环纹称之为“红腰狸”“九节狼”,凉山彝族猎人叫它为“洛布料”(译音),其皮张商品名称为“金狗皮”。     

论横斑钝头蛇是横纹钝头蛇的次定同物异名

M．Smith(1943：116)根据横纹钝头蛇背鳞平滑和横斑钝头蛇背鳞起棱为鉴别特征来检索此二种。又在对横斑钝头蛇的描述中指出二者的主要区别是后者背中部3～7行起棱和半阴茎特征的不同(M．Smith,1943：118)。现根据观察标本及文献记述,对横斑钝头蛇的有效性加以讨论。     

兰科植物区系中一些有意义属的地理分布格局的研究

本文从兰科植物中一些属于中国-喜马拉雅植物亚区和中国-日本植物亚区典型分布属(或亚属)的地理分布格局的研究,提出此两个植物亚区在我国四川省境内是以峨眉山和岷江为其分界,及这条线的走向是从南坪(九寨沟)、松潘(黄龙寺)、茂汶、灌县(光光山)、宝兴、二郎山(天全以西)、峨眉山、石棉、西昌、德昌、米易至攀枝花市。     

兰科植物区系中的一些有意义属的地理分布格局的研究

本文从兰科植物中一些属于中国－喜马拉植物亚区和中国－日本植物亚区典型分布属（或亚属）的地理分布格局的研究,提出此两个材区在我国四川省境内是以峨眉山和岷江为其分界,及这条线的走向是从南坪（九寒寨沟）、松潘（黄龙寺）、茂汶、灌县（光光山）、宝兴、二郎山（天全以西）。峨眉山、石棉、西昌、德昌、米易至攀枝花市。     

珍贵动物资源调查简讯

四川省南充师范学院生物系革命师生,在毛主席关于“世上无难事,只要肯登攀”教导的巨大鼓舞下,结合珍贵动物资源调查进行开门办学。1976年2月下旬,生物系百余人,对天仓、宝兴、越西三县的珍贵动物进行了调查。工作中实行教学、科研、生产劳动三结合,并宣传保护珍贵动物资源的意义及如何识别珍贵动物等,做到既是调查队,也是宣传队。通过半年的野外调查工作,胜利地完成了三县10,719公里的珍贵动物资源调查任务。初步查清了大熊猫、金丝猴、羚牛、鬣羚、     

四川省鸡形目鸟类及分布

研究使用了从1997~2005年四川省58个生物资源综合科学考察的鸟类野外调查记录、国内外从1908年到2005年有关四川省鸡形目鸟类野外物种调查公开发表的60篇文献,以及四川大学、西华师范大学、四川农业大学标本馆共386号鸡形目标本,共1103条记录,对四川省鸡形目的资源及分布进行了研究。结果如下:①四川省鸡形目鸟类计2科18属25种,占全国鸡形目(63种)的39.68%,其中,国家I级保护鸡形目5种,国家II级保护鸡形目11种,分别占全国保护鸡形目总数(I级21种、II级21种)的23.81%、52.38%;特有种7种,占全国鸡形目特有种(14种)的50%,分布型以喜马拉雅-横断山区型居多,以东洋界成分占绝对优势。②物种丰富度最高的地区为平武县、松潘县、九寨沟县、绵竹县、冕宁县、康定县、青川县和宝兴县,保护物种丰富度最高的地区为平武县、九寨沟县、宝兴县、松潘县、青川县、冕宁县;③四川省159个县(市)中有鸡形目调查记录的有73个,没有记录的县86个,这86个空白区域有待进一步调查。     

四川邛崃山系小熊猫分布

2000-2002年,对四川邛崃山系11个县(市)的小熊猫分布状况进行了调查.共完成调杏样线2332条,发现有小熊猫痕迹样线130条,分布在9个县,样线平均遇见率为5.57%,样线遇见率最高的是汶川县和崇州市;估算整个山系有小熊猫栖息地面积5107.82km2,以宝兴、汶川和天全3县的面积最大,分别占总面积的34.08%、30.65%和16.61%,这3个县占总面积的81.34%;小熊猫分布密度等级最高的区域是崇州市的四川鞍子河自然保护区.在山系内7个自然保护区中有小熊猫栖息地面积2166.84km2,占栖息地总面积的42.4%.小熊猫空间分布格局极不连续,存在许多空白区域,栖息地存在破碎化趋势,应进一步加强对邛崃山世界遗产地内小熊猫种群的保护.     

Structure of the gut of the racer goby Neogobius gymnotrachelus (Kessler, 1857)

The morphological and histological features of the gut of juvenile racer goby Neogobius gymnotrachelus [range of body mass 0·2–0·9 g and standard length ( L _S) 20·0–38·8 mm] were examined in fish collected from the Włocławek Reservoir on the Vistula River, Poland. Evidence is provided of the stomachless nature of the gut of the racer goby. The intestine of the juveniles lacks the intestinal bulb. A particular feature of the racer goby gut is the secretory oesogaster (the transitional region where the secretory oesophagus merges into the intestine) with multicellular alveolar glands that secrete via a common duct to the surface between the mucosal folds. The cells in the secretory oesogaster alveolar glands are periodic-acid-Schiff (PAS) positive indicating the presence of neutral mucopolysaccharides. It is hypothesized that the secretory oesogaster is evidence of the loss of the functional stomach in this species. There are two sphincters: the oesogaster–intestinal and the intestinal–rectal in the racer goby gut. It can be concluded that the oesogaster–intestinal sphincter is functionally related to the gastro-intestinal sphincter described in many fish species having a stomach. Enterocytes with a distinct brush border and only a few goblet cells are observed in the epithelium along the whole intestine. The existence of vacuoles in the supranuclear region of the rectal enterocytes is also confirmed.     

笼养绿尾虹雉繁殖参数的观察与分析

观察并分析了1993 ～2011年四川蜂桶寨国家级自然保护区笼养绿尾虹雉的产卵数、受精率和产卵持续天数等3个繁殖参数.结果发现,笼养绿尾虹雉的雌鸟平均产卵数为3.62枚(n=19),平均产卵天数为41.63 d(n=19),平均受精率为14.13％ (n=19).与北京笼养种群相关数据比较,其产卵数、产卵持续天数没有显著差异(均P ＞0.05),但受精率远低于北京种群(P＜0.05).     

Lateral packing of protofibrils in fibrin fibers and fibrinogen polymers

The distinctive transverse banding pattern of fibrin fibers clearly indicates ordering of molecules in the longitudinal direction. In this study we examined the fibers of fibrin clots, as well as two types of fibrinogen polymers, by thin-section electron microscopy. The fibrinogen polymers have a transverse banding pattern identical to that of fibrin fibers—clearly indicating a regular longitudinal repeat—but they are larger in diameter, and show little or no branching. We therefore expected their overall ordering to be better than that of fibrin fibers. Several different fixation protocols were used. We readily observed the typical transverse banding seen previously by negative stain and metal replication techniques. However, only very rarely was any regular lateral lattice seen in any of the samples. X-ray diffraction was used to examine unfixed specimens of the two fibrinogen polymers and, once again, although a longitudinal repeat was evident, only rarely was evidence for lateral crystallinity seen. The electron-microscope and x-ray results showed that the needles and pellet fibers of fibrinogen have essentially the same internal architecture as thick fibrin fibers, and that all three types of polymer, although clearly transversely banded, have almost no crystallinity in their lateral protofibril packing.     

绿尾虹雉的繁殖生态研究

本文对我国珍禽绿尾虹雉(Lophophorus lhuysll)的栖息地类型,繁殖期的活动规律、巢和卵、种群密度,及天敌等做了报道,为在原产地列野生种群繁殖习性的首次记述;并对绿尾虹雉野生群体的繁殖比率、配偶方式及该物种的濒危程度问题做了理论性探讨。     

Genetic and biogeographic relationships of the racer goby Neogobius gymnotrachelus (Gobiidae: Teleostei) from introduced and native Eurasian locations

The racer goby Neogobius gymnotrachelus , along with several other neogobiin fishes, has been spreading north and west from its native Ponto-Caspian range for the past two decades via shipping and canals. It has been predicted as a likely future invader of the North American Great Lakes, where it would join its neogobiin relatives – the round and freshwater tubenose gobies. The present study is the first to analyse the population genetic and biogeographic relationships of the racer goby, establishing a baseline to aid interpretation of its future spread patterns and likely donor-recipient population relationships. The mitochondrial DNA cytochrome b gene was sequenced from representative areas of the racer goby's range, including rivers of the northern Black Sea and areas of spread upstream in the Danube River and outside the Ponto-Caspian region to the Vistula River in Poland. Results discerned nine haplotypes, with few shared among drainages of native rivers and most sites housing unique alleles. Racer goby populations significantly diverged among watersheds, supporting historic low migration and little non-anthropogenic gene flow. The Dnieper River was identified as a likely donor source for the Vistula River colonization, where appreciable variability suggests a relatively large number of founding genotypes.     

Diet partitioning in summer of two syntopic neogobiids from two different habitats of the lower Vistula River, Poland

Monkey goby Neogobius fluviatilis and racer goby N. gymnotrachelus, both native to the Ponto‐Caspian region, were discovered in Polish rivers during the mid‐1990s and currently they are invading the River Vistula. Preliminary studies were carried out on the diet of both species at two sites in the lower River Vistula, one lotic (main channel of the river downstream of the W?oc?awek river reservoir) and the other lentic (left bank, middle section of the W?oc?awek Reservoir). Significant differences were noted in diet between the two species and within species according to habitat, in particular the types of chironomid taxa taken. Over a single diel cycle, monkey goby were captured between 07:00 and 19:00 hours only, feeding more intensively on chironomid larvae than the racer goby, which was captured at most times of day. The wide diet plasticity of these non‐native fish species favours their expansion.     

Diel-feeding activity in early summer of racer goby Neogobius gymnotrachelus (Gobiidae): a new invader in the Baltic basin

The first recording of the Ponto‐Caspian racer goby in Poland was during 1995 in the River Bug (River Vistula system). Within 5 years, the species had spread to the downstream section of the Vistula. One of the potential impacts of invasive species on native fauna is competition for food. Therefore, the diel patterns in diet composition and gut fullness coefficient (FC) of racer goby were examined at one study site in the W?oc?awski Reservoir (lower River Vistula), during May 2003. An average of 20 individuals were examined each 4 h over one 24‐h period (125 fish in total). The proportion of main food items and diet width did not differ among three size‐groups (43–59, 60–79 and 80–97 mm total length), and the relative biomass ratio of main food categories did not differ over the diel cycle. Amphipods constituted 11–70% of total gut content biomass and were found on average in 84% of analysed alimentary tracts. The second prey types were chironomid larvae (16–63% of total food biomass; frequency occurrence: 61–91%), and to a lesser extent chironomid pupa, ceratopogonid larvae, oligochaets, dipteran imagines and copepods, with fish larvae found in the gut of eight gobies. Gut fullness coefficient (FC) differed significantly over the 24‐h period, with the highest value at night and in early morning. In conclusion, racer goby forages mainly on benthos and has a nocturnal‐feeding activity. No significant diet overlap was found between racer goby and native percids, i.e. Eurasian perch Perca fluviatilis and ruffe Gymnocephalus cernuus.     

四川美姑、汉源黑颈鹤迁徙停歇地环境调查

于2005年及2006年春季在云南大山包和贵州草海给8只黑颈鹤(Grus nigricollis)放置了卫星发射器,观察结果表明,其中6只黑颈鹤至少完成了一次完整的春季或秋季迁徙。黑颈鹤在四川省汉源停歇的乡镇包括:乌斯河镇、河南、坭美、梨园、宜东、三交坪和皇木;在美姑停歇的乡镇包括:柳洪、依果觉、农作、拉木阿觉、炳途、瓦古、峨曲古龙门和尼哈。2005年6月4~8日和2006年6月8~14日实地考察了汉源和美姑两县的黑颈鹤停歇地点。黑颈鹤在两县的停歇地点都在海拔1900m以上且人为活动稀少的区域,或为草甸覆盖的高山浑圆山头,或为高山缓坡的林间空旷草地。