| 达摩兰组织培养适宜培养基的研究 |
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| 引用本文： | 何官榕,何碧珠.达摩兰组织培养适宜培养基的研究[J].亚热带植物科学,2009,38(4):62-65. |
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| 作者姓名： | 何官榕 何碧珠 |
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| 作者单位： | 1. 福建农林大学,植保学院,福建,福州,350002
2. 福建农林大学,园艺学院,福建,福州,350002 |
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| 摘 要： | 以达摩兰的原种苗茎段为外植体进行组织培养研究，筛选出最适培养基（1）原球茎诱导：MS＋6-BA 0．5mg／L＋NAA 1．0mg／L＋香蕉肉250g／L；（2）丛生芽诱导：1／2MS＋6-BA 3mg／L＋NAA 2．0mg／L＋AC 2．5g／L＋蔗糖3％＋香蕉肉200g／L；（3）芽增殖：1／2MS＋6-BA 3mg／L＋NAA 2．0mg／L＋AC 2．5g/L＋蔗糖3％＋香蕉肉150g／L；（4）根诱导：1／2MS＋6-BA 0.3mg／L＋IBA 0．5mg／L＋NAA 0．5mg／L＋AC 2．5g／L＋蔗糖3％。采用珍珠岩、细石与树皮的混合物为培养基质，移栽成活率达95％以上。
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| 关 键 词： | 达摩兰 茎段 组织培养 外植体 |
Study of Tissue Culture Technology in Dharma Orchid |
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| HE Guan-rong,HE Bi-zhu.Study of Tissue Culture Technology in Dharma Orchid[J].Subtropical Plant Science,2009,38(4):62-65. |
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| Authors: | HE Guan-rong HE Bi-zhu |
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| Abstract: | The trial used the original seedling stems of dharma orchid for tissue culture technology study, selected out the optimum medium:(1) protocorm induced: MS + 6-BA 0.5 mg/L + NAA 1.0 mg/L + banana 250 g/L;(2) bud induced: 1/2 MS + 6-BA 3 mg/L + NAA2.0 mg/L + AC 2.5 mg/L + su 3% + banana 200g/L;(3) shoot proliferation: 1/2 MS + 6-BA3 mg/L + NAA 2.0 mg/L + AC 2.5 mg/L + su 3% + banana 150g/L;(4) rooting: 1/2 MS + 6-BA 0.3 mg/L + IBA 0.5 mg/L+NAA 0.5 mg/L + AC 2.5 mg/L + su 3%.Using mixture of perlite, fine stone and bark as culture substratum, the survival rate was up to 95%. |
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| Keywords: | dharma orchid stem tissue culture explant |
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