幽门螺杆菌相关蛋白在脑梗死患者血清中的表达

目的探讨幽门螺杆菌(Helicobacter pylori,H.pylori)细胞毒素相关蛋白A(CagA)在动脉粥样硬化性脑梗死患者血清中的表达。方法对脑梗死组65例患者的中性粒细胞、血纤维蛋白原、hsCRP及CagA-Hp-IgG抗体进行测定,采用超声检测颈部血管,所有检测结果与健康对照组进行比较。结果研究组与对照组血清CagA-Hp抗体阳性率分别为60%和32%;H.pylori抗体阳性组较阴性组相比,血hs-CRP和纤维蛋白原升高明显(t=4.872,P0.05;t=1.982,P0.05);H.pylori抗体阳性者狭窄及颈动脉斑块发生率分别为53.8%和89.7%,与对照组的26.9%和53.8%相比差异具有统计学意义(χ2=5.826和6.297,P均0.05)。结论动脉粥样硬化性脑梗死患者的H.pylori感染率高于健康人,H.pylori感染可能与脑梗死的发生有关。     

326例儿童缺铁性贫血与感染幽门螺杆菌关系探究

摘要：目的 分析幽门螺杆菌（H.pylori）对儿童缺铁性贫血（IDA）的影响,探讨H.pylori感染与儿童IDA的相关性,提高儿童IDA诊治率。方法 应用13C-呼气试验和胶体金法检测326例IDA患儿及211例健康儿童H.pylori感染情况,并对其结果进行统计学分析。结果 326例IDA患儿中H.pylori检测阳性217例（阳性率为66.56%）,健康组儿童H.pylori检测阳性47例（阳性率为22.27%）,两组比较差异有统计学意义（χ2=100.54,P<0.01）。2－6岁组分别与7－9岁组及10－12岁组H.pylori阳性率比较差异均有统计学意义（χ2=8.61、21.46,P<0.05）,7－9岁组较10－12组H.pylori阳性率比较差异无统计学意义（χ2=2.62,P>0.05）。其中女患儿H.pylori阳性率为78.06%（121/155）,男患儿H.pylori阳性率为56.14%（96/171）,两者比较差异有统计学意义（χ2=17.55,P<0.01）,且不同年龄组男女阳性率比较差异均有统计学意义。217例H.pylori感染的IDA患儿以轻度贫血为主,占95.85%（208/217）。结论 H.pylori感染与儿童IDA之间存在相关性。     

229例慢性胃炎患者幽门螺杆菌培养及耐药情况

目的了解慢性胃炎患者H.pylori感染及其耐药情况,为临床治疗提供参考。方法慢性胃炎患者胃镜活检标本培养分离H.pylori,对分离的H.pylori采用纸片扩散法进行耐药性检测。结果229例患者分离出97株H.pylori;H.pylori分离阳性率为42．36％（97／229）,其中男性分离率为43．79％（67／153）,女性分离率为39．47（30／76）;92株H.pylori对抗生素的耐药性分别为：甲硝唑8．7％,克拉霉素7．6％,阿莫西林1．1％、呋喃唑酮1．1％,阿奇霉素4．4％,左氧氟沙星0％。结论慢性胃炎患者H.pylori感染率较高,但与性别、年龄无关;慢性胃炎H.pylori对常用抗生素敏感,建议采用左氧氟沙星、阿莫西林、呋喃唑酮进行治疗。     

326例儿童缺铁性贫血与感染幽门螺杆菌关系探究

目的分析幽门螺杆菌(H.pylori)对儿童缺铁性贫血(IDA)的影响,探讨H.pylori感染与儿童IDA的相关性,提高儿童IDA诊治率。方法应用~(13)C-呼气试验和胶体金法检测326例IDA患儿及211例健康儿童H.pylori感染情况,并对其结果进行统计学分析。结果 326例IDA患儿中H.pylori检测阳性217例(阳性率为66.56%),健康组儿童H.pylori检测阳性47例(阳性率为22.27%),两组比较差异有统计学意义(X~2=100.54,P0.01)。2-6岁组分别与7-9岁组及10-12岁组H.pylori阳性率比较差异均有统计学意义(X~2=8.61、21.46,P0.05),7-9岁组较10-12组H.pylori阳性率比较差异无统计学意义(X~2=2.62,P0.05)。其中女患儿H.pylori阳性率为78.06%(121/155),男患儿H.pylori阳性率为56.14%(96/171),两者比较差异有统计学意义(X~2=17.55,P0.01),且不同年龄组男女阳性率比较差异均有统计学意义。217例H.pylori感染的IDA患儿以轻度贫血为主,占95.85%(208/217)。结论H.pylori感染与儿童IDA之间存在相关性。     

幽门螺杆菌感染与慢性口臭关系的初步研究

目的 调查主诉口臭患者的幽门螺杆菌（H．pylori）感染率和主诉消化不良的口臭发生率。方法 研究对象为125例主诉慢性口臭患者和212例主诉慢性消化不良患者。口臭以口气挥发性硫化物（VSC）检测与闻诊联合诊断,H．pylori感染以^14C-尿素呼气试验诊断。结果 125例主诉慢性口臭的患者有87例是真性口臭,其余38例为假性口臭,真性口臭患者的H．pylori感染率显著高于假性口臭（40．2％和13．2％,P〈0．01）。212例主诉慢性消化不良的患者发生口臭105例（49．5％）、感染H．pylori 94例（44．3％）,H．pylori阳性患者的口臭发生率显著高于H．pylori阴性患者（57．5％和43．2％,P〈0．05）。无论何种主诉,大部分口臭患者属于VSC阳性（88．5％）,但H．pylori阳性患者和H．pylor阴性患者口气VSC水平差异无显著性,VSC阳性口臭和VSC阴性口臭的H．pylori感染率差异也无显著性。结论 H．pylori感染可能与口臭的发生有一定关系,但口气VSC并非由H．pylori直接产生。     

胃息肉与幽门螺杆菌感染关系研究

目的探讨胃息肉与幽门螺杆菌（Helicobacter pylori,H.pylori）感染关系。方法对1218例胃息肉同时进行H．pylori检查患者进行回顾性分析,分析胃息肉患者H.pylori感染率、胃息肉部位与H.pylori感染关系、胃息肉病理类型与H.pylori感染关系。结果发现胃息肉Hpylori感染患者532例,Hpylori感染率为43．7％。男性胃息肉患者H.pylori感染率为47．5％（216／455）,女性Hpriori感染率感染率为41．4％（316／763）（P〉0．05）,年龄〈20岁、20～39岁、40—59岁和≥60岁胃息肉H.priori感染率分别为41．7％、44．7％、41．6％和47．2％（P〉0．05）;胃窦胃角息肉H.pylori感染率高于其他部位（胃体、胃底和贲门）（P〈0．05）;炎性和增生性胃息肉H.priori感染率高于胃底腺和腺瘤性息肉（P〈0．05）。结论H.pylori感染可能与部分胃息肉发生有一定关系,需要进一步深入研究胃息肉的发生机制。     

汉中市汉台区无症状儿童幽门螺杆菌感染率的 流行病学调查

目的研究陕西省汉中地区无症状儿童幽门螺杆菌(H.pylori)感染的流行病学特点及其影响因素。方法采用酶联免疫吸附法(ELISA)检测2013年1月至2018年1月间我院328例无消化道症状儿童Hp-IgG水平情况,分析其流行病学特点,并行问卷调查研究幽门螺杆菌感染的影响因素。结果 328例无消化道症状儿童H.pylori感染率为18.90%。其中男童169例,感染率21.89%;女童159例,感染率15.72%;城市儿童137例,感染率20.44%;农村儿童191例,感染率17.80%。经Logstic回归分析,年龄3～4岁、饭前便后洗手、父母具有大学文化水平均为H.pylori感染的保护性因素;啃手指、公用洗漱杯、直系亲属胃病史均为H.pylori感染的独立危险因素,差异均有统计学意义(P0.05)。结论 H.pylori感染在儿童中较为常见,生活中不良习性与H.pylori感染密切相关。H.pylori感染相关高危因素的分析对于儿童H.pylori感染的防控具有重要临床意义。     

儿童再发性腹痛中幽门螺杆菌不同检测方法

目的 评价幽门螺杆菌（H.pylori）的不同检测方法及H.pylori感染在儿童再发性腹痛（RAP）诊断中的应用.方法 对在我院诊治的儿童RAP 72例,分为学龄期组24例,少年组48例,同时进行细菌培养、快速尿素酶试验、病理组织学检查、血清学H.pylori抗体检测和粪便H.pylori抗原（HpSA）检测.将前3项检查中2项阳性或细菌培养一项阳性作为诊断H.pylori感染的金标准.结果 金标准诊断H.pylori感染,阳性占37例,阴性占35例,总阳性率为51.4%;学龄期儿童组H.pylori检出阳性率为37.5%,少年组H.pylori检出阳性率为58.3%,两组比较差异有显著性（P＜0.01）;比较6种不同检测方法的敏感性、特异性、准确性,经x2检验,差异均无显著性（P＞0.10）.结论 H.pylori感染与儿童RAP之间存在一定的联系,少年组H.pylori感染率高于学龄期儿童组,而粪便HpSA检测有较高的敏感性和特异性,是一种简便可靠,非侵入性的诊断H.pylori感染的方法,尤其适合儿童.     

胃癌发生不同阶段临床和胃镜表现及与幽门螺杆菌感染的关系

目的观察慢性浅表性胃炎(CSG)→萎缩性胃炎(CAG)→肠上皮化生(IM)→非典型增生(DYS)→胃癌(GC)五个不同阶段临床和胃镜表现及其与幽门螺杆菌(H.pylori)感染的关系。方法对经胃镜及病理证实的上述GC发生五个不同阶段的患者各50例,详细询问其临床症状,记录胃镜下表现,并用快速尿素酶试验(RUT)、14C尿素呼气试验(14C-UBT)、组织学检查及H.pylori培养等方法检测H.pylori感染情况。结果 (1)CSG和GC的临床表现分别以上腹胀和早饱、体重减轻为主,其他阶段均以上腹痛为主;(2)CSG、CAG和GC的胃镜下表现依次以疣状隆起及糜烂、黏膜变薄及血管显露、巨大溃疡及出血为多见,而IM和DYS均以直径小于2 cm的溃疡为多见(P0.05);(3)H.pylori感染:从CSG→GC不同阶段,H.pylori感染率依次降低(分别为80%、78%、62%、56%和36%,P0.05);临床表现中上腹痛及纳差H.pylori感染率高,早饱H.pylori感染率低(P0.05);不同胃镜下表现中溃疡H.pylori感染率最高(P0.05)。结论体重减轻可作为胃癌警示信号,胃镜下巨大溃疡及出血以GC多见;H.pylori感染率与GC发生阶段呈负相关,临床表现为上腹痛、纳差,及胃镜下溃疡患者H.pylori感染率高。     

幽门螺杆菌感染诊断方法的比较

目的:考核各种H.pylori感染诊断方法,试图证明分离培养仍是细菌性感染诊断的最可靠方法.方法:315例胃十二指肠疾病患者同时进行胃活检组织分离培养,病理切片找菌,快速脲酶试验,14CO2呼气试验检查,比较它们之间的检出率差异.结果:四种方法各自的总检出率差异无显著性.以分离培养为金标准,其他方法的误诊误治率可达12.06%～22.22%.各种诊断方法对H.pylori感染的实际检出率差异存在着显著性.结论:本研究提示,分离培养应为H.pylori感染临床诊断的金标准.     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Entwicklungsgeschichte und Ultrastruktur von Pollenkitt und Exine bei nahe verwandten entomophilen und anemophilen Angiospermensippen derAlismataceae,Liliaceae, Juncaceae,Cyperaceae, Poaceae undAraceae

Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.

     

Identification and Characterization of the First Equine Parainfluenza Virus 5

正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various