RP-HPLC 法测定酸枣仁汤中芒果苷的含量

目的:建立测定酸枣仁汤中芒果苷含量的反高效液相色谱分析方法。方法:色谱柱:WatersC18(250nm×4.6mm,5μm);流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;流速:1.0ml/min,检测波长:257nm。结果:芒果苷在0.316μg-1.264μg范围内与峰面积线性关系良好(r=0.9999),平均加样回收率为97.64%,RSD为0.36%。结论:本方法简便、准确、可靠、重复性好,可作为酸枣仁汤中芒果苷的含量测定方法。     

RP-HPLC法测定酸枣仁汤中甘草苷的含量

目的：建立测定酸枣仁汤中甘草苷含量的反相高效液相色谱分析方法。方法：色谱柱：WatersC18（250nm×4．6mm,5wm）;流动相：乙腈一0．1％磷酸水溶液,梯度洗脱;流速：1．0ml／min,检测波长：276nm。结果：芒果苷在0．093μg·0．744μg范围内与峰面积线性关系良好（r==0．9999）,平均加样回收率为98．04％,RSD为0．41％。结论：本方法简便、准确、可靠、重复性好,可作为酸枣仁汤中甘草苷的含量测定方法。     

RP-HPLC法测定酸枣仁汤中芒果苷的含量

目的：建立测定酸枣仁汤中芒果苷含量的反高效液相色谱分析方法。方法：色谱柱：WatersC18（250nm×4.6mm,5μm）;流动相：乙腈-0.1%磷酸水溶液,梯度洗脱;流速：1.0ml/min,检测波长：257nm。结果：芒果苷在0.316μg-1.264μg范围内与峰面积线性关系良好（r=0.9999）,平均加样回收率为97.64%,RSD为0.36%。结论：本方法简便、准确、可靠、重复性好,可作为酸枣仁汤中芒果苷的含量测定方法。     

HPLC法测定甘草酸和甘草苷的含量

目的:建立以HPLC法同时测定甘草中甘草酸和甘草苷含量的方法.方法:采用C18柱(4.6mmx15cm,5μm)为分析柱;以乙腈-1%醋酸溶液为流动相;流速为0.6ml·min-1;柱温25℃;采用外表法定量测定.结果表明甘草酸在4.16-28.0μg/ml范围内,回归方程为Y=10562.8X+30963(r=0.9999);甘草苷在13.0-23.4μg/ml范围内,回归方程为Y=12857.4X+16437(r=0.9997),平均加样回收率均大于96.08%,日内、日间的RSD值分别小于1.41%和1.85%.结论本方法操作简单、准确、快速、重现性好,其它组分干扰少,可用于甘草酸和甘草苷的含量测定.     

复方甘草片HPLC指纹图谱及7成分测定

本文建立了复方甘草片的HPLC指纹图谱及吗啡、磷酸可待因、芹糖甘草苷、甘草苷、苯甲酸钠、异甘草苷、甘草酸7成分测定方法。采用50%甲醇水溶液对复方甘草片提取后,用HPLC-UV法进行测定;色谱条件为:Welch Ultimate AQ-C_(18)色谱柱(5μm,4.6 mm×250 mm);乙腈-0.02 mol/L磷酸盐缓冲液(pH4.0)为流动相,梯度洗脱;流速1.0 mL/min;检测波长220、254 nm;柱温35℃;进样量10μL。采用国家药典委员会"中药色谱指纹图谱相似度评价系统"(2012年版)建立18批样品指纹图谱,并同时测定7成分含量。结果显示18批复方甘草片指纹图谱相似度均大于0.95,共有27个共有峰,通过与对照品对照保留时间鉴定了14个共有峰。吗啡、磷酸可待因、芹糖甘草苷、甘草苷、苯甲酸钠、异甘草苷、甘草酸在各自浓度范围内线性关系良好(r0.999 0),平均加样回收率94.73%～104.45%,RSD 0.68%～3.89%。本方法简便、快速、准确,可用于复方甘草片的质量控制。     

HPLC法测定酸枣仁颗粒中斯皮诺素的含量

目的:建立测定酸枣仁颗粒中斯皮诺素含量的高效液相色谱方法。方法:采用Agilent C18柱(4.6×250mm 5μm),乙腈-水(20:80)为流动相洗脱,流速为1.0mL/min,检测波长为335nm。结果:斯皮诺素在0.252μg~2.518μg范围内线性关系良好,平均回收率符合含量测定要求。结论:本法简单易行,结果准确可靠,可有效地用于酸枣仁颗粒的质量控制。     

HPLC 法测定不同来源槐角中槐角苷含量

目的:建立HPLC法测定槐角中槐角苷含量测定方法并检测不同来源槐角中槐角苷的含量。方法:Luna 5u C18(2)100A柱(250 mm×4.60 mm,5μm),柱温为35℃,流动相为甲醇-乙腈-1%冰醋酸(40:5:55),流速为1.0 ml/min,检测波长为260 nm。结果:槐角苷质量浓度在14.58 mg/L～53.46 mg/L范围内呈良好的线性关系,回归方程为y=110353x-274717(r=0.9999),平均回收率为99.5%,RSD为1.27%;不同来源槐角中槐角苷含量范围4.27%～8.78%。结论:该方法简便、快速、灵敏、可靠;不同来源的槐角中槐角苷含量有较大差异,其中以陕北黄土高原品种含量最高,可为槐角的质量保证提供依据。     

黄芪桂枝五物颗粒中芍药苷的含量测定

目的:建立一个稳定可靠分离度好的方法测定黄芪桂枝五物颗粒中芍药苷的含量。方法:采用高效液相色谱法,色谱柱:YMC-Pack ODS-A(250×4.6mm,5μ);流动相:以乙腈—0.1%磷酸溶液(14:86)为流动相;检测波长:230nm;流速为1.0ml/min;柱温为30℃。结果:芍药苷的进样量在0.120μg~1.200μg范围内有良好的线性关系,平均回收率为99.22%,RSD为0.89%。建立了高效液相色谱法测定黄芪桂枝五物颗粒的含量的方法,该方法准确、可靠、分离度好。     

高效液相色谱法测定降糖1号胶囊中淫羊藿苷的含量

目的:建立测定降糖1号胶囊中淫羊藿苷含量的方法。方法:室温条件下超声提取降糖1号胶囊中的淫羊藿苷,用D101大孔吸附树脂柱纯化提取物,高效液相色谱法(HPLC)测定含量,色谱柱:Hypersil ODS2(4.6mm×200mm,5μm);流动相:乙腈-水(30:70);流速:1ml/min,检测波长270nm;柱温:30℃。结果:淫羊藿苷的进样量在0.08μg-0.28μg范围内线性关系良好(r=0.9999),平均加样回收率(n=6)为96.66%,RSD为2.14%。结论:该方法简便、准确、重复性好,可用于降糖1号胶囊的质量控制。     

HPLC法测定利鼻片中黄芩苷的含量

目的:采用HPLC法测定利鼻片中黄芩苷的含量。方法:色谱柱:Agilent C18(5μm,250×4.6mm);流动相:甲醇-水-磷酸(43:57:0.2);检测波长:280nm。结果:黄芩苷在0.13~4.16μg范围内呈良好的线性关系(r=0.9999997),平均回收率为100.44%,RSD=1.49%。结论:采用本法测定利鼻片中黄芩苷的含量,操作简便,准确可靠,可用于该药品的质量控制指标之一。     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Comparison of in vitro decomposition of bryophytic and tracheophytic plant material

The fossil record of bryophytes is generally poor and infertile plants attributable to liverworts and mosses could also be thalloid vascular plant gametophytes or herbaceous lycophytes respectively. The paucity of the bryophytic record could be the result of relatively rapid degradation of bryophytic material in comparison to that of vascular plants, the absence of lignified cells in bryophytes sustaining this belief. However, certain organs of bryophytes are as robust as those of vascular plants (e.g. spores) and the hydroxybenzofuran polymers produced by some mosses could be of similar preservational resistance to lignins. A simplistic experiment to test the relative resistance to decay of bryophytic material has been undertaken. Its findings suggest that bryophytic plant material has a similar preservational potential to that of a selected vascular plant, particularly in organic rich sediments. This result may be construed to imply that bryophytes were indeed less abundant in the preservational environments of the Coal Measures than might be expected on the basis of current palaeoenvironmental reconstructions.     

Epidemiological,Clinical and Serological Characteristics of Children with Coronavirus Disease 2019 in Wuhan: A Single-centered,Retrospective Study

In December 2019, SARS-CoV-2 was first detected in the samples obtained from three adult patients who suffered from an unknown viral pneumonia in Wuhan (Li et al. 2020). This unknown viral pneumonia is further named as coronavirus disease 2019 (COVID-19) by the World Health Organization. To date, the number of new COVID-19 cases has continued to skyrocket and the impact of SARS-CoV-2 on humans is far greater than any pathogen of this century in both breadth and depth. Previous studies have shown that adults with COVID-19 have symptoms of fever, dry cough, dyspnea, fatigue and lymphocytopenia. Moreover, COVID-19 is more likely to cause death in the elderly, especially those with chronic comorbidities (Huang et al. 2020). In Wuhan, more than 50, 000 COVID-19 cases have been confirmed, including over 780 pediatric patients, and only one child death case (Lu et al. 2020). Although the number of children cases was far fewer than that of adults, COVID-19 might endanger children's health and the information on children remains limited, especially in serological study. In the retrospective study, the investigators analyzed the epidemiological, clinical and serological characteristics of children with COVID-19 in Wuhan in the early stages of the outbreak, which might provide theoretical and practical help in controlling COVID-19 and similar emerging infectious diseases in the future.