麻醉诱导时血压下降对术后恶心呕吐的影响

目的观察麻醉诱导时血压下降与术后恶心呕吐的关系。方法选择ASAⅠ-Ⅱ级的经腹胆囊切除术患者40例,按麻醉诱导时血压比基础值下降的程度分为2组,每组20例。A组SBP比基础血压下降>30%;B组SBP比基础血压下降<30%。术后随访病人48小时,记录病人恶心呕吐的发生情况。结果麻醉诱导时血压下降对术后恶心呕吐有明显影响。结论麻醉期间维持血流动力学稳定能降低PONV的发生。     

非阿片类镇痛复合静脉全麻在鼾症手术患者中的疗效

目的:观察三重措施预防为基础,联合非阿片镇痛药复合静脉全麻在行鼾症手术患者术后恶心呕吐的应用效果。方法:选择择期行鼾症手术男性病人80例,随机分为两组:吸入麻醉组(inhalation group, IHLA组)和静脉麻醉组(intravenous group, TIVA组),每组40例,两组均采用三重措施预防恶心呕吐,IHLA组采用以舒芬太尼为基础复合七氟烷吸入麻醉,TIVA组以氯胺酮和右美托咪定镇痛基础上丙泊酚全凭静脉麻醉。评估两组病人恶心呕吐危险系数,采用李克特量表(Likert scale),记录并分析两组患者术后6~8 h在麻醉后监测治疗室(post anesthesia care unit, PACU)及病房24 h恶心呕吐发生情况及补救用药用量。结果:两组患者一般临床资料、恶心呕吐风险评分、手术时间、术后恢复期补救用药量人数无显著差异(P>0.05);IHLA组在PACU恶心呕吐发生率为39.5%,TIVA组发生率为18.9%,两者相比有显著性差异(P<0.05);IHLA组病房24 h恶心呕吐严重程度高于TIVA组,两组术后需要补救应用抗呕吐药物用量无显著差异(P>0.05)。结论:以三重措施预防为基础,与吸入麻醉相比,非阿片类镇痛药复合静脉麻醉可以减少肥胖病人鼾症手术术后恶心呕吐发生率和严重程度,降低围术期风险,有利于患者早期恢复。     

高血压手术患者对不同麻醉诱导的反应研究

目的:比较高血压手术患者在不同麻醉诱导过程中,分别使用异丙酚、七氟醚及两种联合下的麻醉诱导对病人血压、心率及麻醉深度指数(CSI)的影响,以寻求最理想的针对高血压手术患者的麻醉方法.方法:本研究选择临床确诊ASA Ⅱ级,高血压Ⅰ～Ⅱ级择期全麻手术患者90例,随机分为三组(A、B、C组),每组30例,分别对应异丙酚、七氟醚及异丙酚联合七氟醚麻醉诱导组.分别观察三组患者麻醉诱导前(T0)、诱导后(T1)、插管后(T2)的心率(HR)、血压(SBP/DBP)及麻醉深度指数(CSI)的变化.其中所有结果均经统计学t检验,P＜0.05有统计学差异.结果:组内比较,三组患者T1和T0比较,SBP和DBP均呈显著性下降(P＜0.05),而T1和T2比较,SBP和DBP均呈显著性上升(P＜0.05),T2和T0比较,A组无明显差异(P＞0.05),B组和C组差异明显(P＜0.05);组间比较,诱导后的血压下降,A组较B下降幅度明显,差异有统计学意义(P＜0.05).A组与C组相比血压下降,差异有统计学意义(P＜0.05).插管后血压上升,B组较A组血压上升,差异有统计学意义(P＜0.05).A组较C组血压上升幅度明显,差异有统计学意义(P＜0.05).B组较C组血压上升幅度明显,差异有统计学意义(P＜0.05).三组患者T1的CSI均无差异性(P＞0.05).A组T2比B组CSI较低,有统计学差异(P＜0.05),C组比B组CSI也较低,有统计学差异(P＜0.05).结论:异丙酚,七氟醚,异丙酚联合七氟醚三种方法均可用于高血压患者的术前麻醉.但相比较而言,异丙酚联合七氟醚对插管后高血压患者的平稳作用优于异丙酚组和七氟醚组.     

瑞芬太尼与舒芬太尼复合异丙酚静脉麻醉在妇科腹腔镜手术的效果比较

目的:比较舒芬太尼、瑞芬太尼在妇科腹腔镜手术麻醉中的优越性.方法:60例行妇科腹腔镜手术的病人随机分成舒芬太尼组(S组):舒芬太尼+异丙酚,瑞芬太尼组(R组):瑞芬太尼+异丙酚.记录病人麻醉诱导前、麻醉诱导时、气管插管后即刻、气腹前、气腹(CO2压力达12cmH2O)后1、30、60min以及拔除气管导管后5min的SBP、DBP、HR、PErCO2;并观察记录病人术毕的清醒恢复时间及术后疼痛、恶心、呕吐的情况.结果:R组在麻醉诱导时、气管插管以及拔除气管导管后5min血液动力学变化较S组明显(p＜0.05或0.01),R组术后意识恢复时间和拔管时间明显早于S组(p＜0.05),S组术后疼痛的发生率明显少于R组(p＜0.05).恶心、呕吐的发生率无明显差异.结论:舒芬太尼在妇科腹腔镜手术麻醉中与瑞芬太尼比较在麻醉诱导时及术毕气管拔管时具有较高的血流动力学稳定性,在麻醉过程中的平稳性二者无显著差异,舒芬太尼在术后疼痛控制方面优于瑞芬太尼,而瑞芬太尼在术后意识恢复上优于舒芬太尼.     

亚麻醉剂量丙泊酚对术后恶心呕吐的作用研究

目的:观察丙泊酚对术后恶心呕吐的的影响.方法:选择ASAI-Ⅱ级蛛网膜下腔和硬膜外联合穿刺麻醉下行妇科全子宫切除手术患者80例,随机分为4组,每组20例.C组为空白对照组,P组为安慰剂对照组,A组为恩丹西酮组,B组为丙泊酚组.结果:A组和B组的恶心和呕吐的发生率明显的低于C组(P＜0.05).P组和C组恶心呕吐的发生率无显著性差异.A组和B组术后恶心呕吐的发生率无显著性差异.结论:丙泊酚可降低椎管内麻醉患者术后恶心呕吐的发生率.     

地塞米松联合昂丹司琼对胸腔镜术后病人静脉自控镇痛PONV的影响

目的:观察地塞米松联合昂丹司琼对胸腔镜术后病人自控静脉镇痛(PCIA)相关恶心呕吐的防治效果。方法:120例ASAⅠ~Ⅱ接受胸腔镜手术的患者,随机分为两组:地塞米松与昂丹司琼联合组(OD组)和昂丹司琼组(O组)。OD组患者在诱导时给予10 mg地塞米松,O组给予等量生理盐水,在手术结束前10分钟时,患者均给予静脉注射8 mg昂丹司琼,术后均行病人自控静脉镇痛(PCIA:生理盐水将2μg/kg舒芬太尼和8 mg昂丹司琼稀释到100 m L)。观察术后48小时内的恶心呕吐的发生率及严重程度。结果:在术后恶心呕吐整体发生率上OD组(53.3%)与O组(60%),差异无统计学意义(P0.05),但在重度PONV发生率上OD组(10%)要显著低于O组(26.7%),差异有统计学意义(P0.05)。结论:地塞米松联合昂丹司琼能有效地降低胸腔镜手术后以舒芬太尼为主的病人自控静脉镇痛所致严重恶心呕吐的发生率。     

BIS监测下两种快通道麻醉方式应用于鼻内镜手术的比较

目的:观察BIS指导两种快通道麻醉在鼻内镜手术中的应用及麻醉效果。方法:选择60例ASAⅠ-Ⅱ级择期行功能性鼻内镜手术(FESS)患者,随机分为七氟醚诱导维持麻醉组(VIMA组)与异丙酚全凭静脉麻醉组(TIVA组)。VIMA组:8%七氟醚,氧流量8L/min,潮气量法吸入诱导,七氟醚维持麻醉;TIVA组:异丙酚2 mg/kg诱导,异丙酚维持麻醉。两组诱导时都静脉注射瑞芬太尼1μg/kg,罗库溴铵0.6 mg/kg,监测TOF值为0、BIS60并维持5 s后行气管插管。术中静脉泵注瑞芬太尼0.2μg·kg~(-1)·min~(-1),分别调整七氟醚和异丙酚维持剂量使BIS值在气管插管后至手术结束前15 min左右保持在40~60之间,手术最后15 min保持于60~70之间。两组术后进行Steward评分,并比较两组各时点SBP、DBP、HR,拔管时间,快通道麻醉成功率和苏醒期不良反应发生率。结果:VIMA组拔管时间(11.60±2.55 min)比TIVA组的(7.13±3.26 min)明显延长(P0.05);TIVA组快通道成功率显著高于VIMA组(P0.05)。两组苏醒期不良反应的发生情况比较差异无统计学意义(P0.05)。结论:异丙酚全凭静脉麻醉用于鼻内镜手术拔管时间比七氟醚诱导维持麻醉短,快通道麻醉效果更好。     

氯胺酮加丙泊酚与芬太尼加丙泊酚用于学龄前 小儿非心脏手术的麻醉效果比较

目的:比较芬太尼加丙泊酚与氯胺酮加丙泊酚用于学龄前小儿非心脏手术的麻醉效果。方法:患儿70例,随机分为两组,F组(n=35),用芬太尼2-3 ug／kg,阿曲库铵0．5 mg／kg,丙泊2-3 mg／kg,静脉注射诱导插管,然后微泵持续注射芬太尼0．03-0．06 ug/kg．min-1,阿曲库铵4-8 ug/kg．min-1,丙泊酚80-150 ug／kg．min-1,K组(n=35),用氯胺酮1-1．5 mg／kg,阿曲库铵0．5 mg/kg,丙泊酚2-3 mg／kg,静脉注射诱导插管,然后微泵持续注射氯胺酮30-60 ug／kg．min-1,阿曲库铵与丙泊酚用量同F组,根据术中的情况予以调整。分别记录两组病例在用药前后的收缩压(SBP)、舒张压(DBP)、心率(HR)、麻醉效果、停药至导管拔除时间,以及拔管后因气道梗阻再次插管等情况。结果:K组病例在麻醉诱导时循环稳定,拔管后无呼吸抑制的发生,但术毕拔管的时间延长,F组病例麻醉诱导时HR、SBP、DBP降低明显,拔管后有3例气管痉挛致呼吸抑制重新插管。结论:氯胺酮复合丙泊酚用于学龄前小儿非心脏手术的麻醉,麻醉效果确切,可控性强,麻醉诱导平稳,术中易于调整,术毕拔管虽时间略为延长,但可避免呼吸抑制的发生,较为安全。     

高龄患者腹腔镜直肠癌根治术不同麻醉方法的比较

目的观察比较3种麻醉方法对高龄腹腔镜直肠癌根治术患者的呼吸循环功能和麻醉效果的影响。方法将45例高龄腹腔镜直肠癌根治术患者随机分成3组,每组15例。EA组,连续硬膜外阻滞麻醉组;GA组,静吸复合全身麻醉组;EGA组,小剂量硬膜外阻滞复合静吸全身麻醉组。观察3组麻醉开始前(T0)、麻醉诱导期(T1)、气管插管时(T2)、拔除气管导管时(T3)的HR、SBP、DBP、MAP、PETCO2、ECG,GA组与EGA组同时监测异氟醚MAC、气道压、手术结束自主呼吸恢复时间、自主呼吸潮气量达标、拔管时间、肌松情况及患者麻醉苏醒时间。结果 EA组有13例麻醉效果优良,SpO295%,PETCO2维持在正常范围;EGA组术毕自主呼吸恢复时间、潮气量达标、拔管时间均明显短于GA组(P0.01)。EA组在麻醉10～15 min后血压下降程度与GA组、EGA组麻醉诱导期血压下降相似(P0.05),EGA组在气管插管/拔管期血压波动不明显,而GA组血压显著升高(P0.01);EA组与GA组麻醉效果相似,EGA组麻醉效果优于GA组(P0.05)。结论 EGA组椎管内局麻药用量、全麻维持药用量、肌松药用量等均相应减少,呼吸循环功能较为稳定,术毕麻醉苏醒快,自主呼吸恢复和潮气量达标迅速,多数患者术毕即可拔除气管导管,是高龄腹腔镜直肠癌根治术患者理想的麻醉方法。     

手术后恶心呕吐的机制及其防治

在过去的几十年里,随着各种手术技术的不断提高和完善,已使外科手术的死亡率大幅降低,这使医生的注意力逐渐转向手术后的并发症和患者的满意程度上,如手术后恶心呕吐。恶心呕吐是术后严重的并发症之一,尽管术后恶心呕吐(Postoperativenausea and vomiting,PONV)是非致命性的并发症,但是PONV可导致严重的后果,并且增加了医疗费用,极大的影响了患者的满意程度。PONV高发率一直困扰着临床医生和患者。所以,探讨其发生及其风险因素是十分必要的。但是,术后恶心呕吐的病理生理学机制还不是十分清楚,本文总结了目前关于术后恶心呕吐发生机制的一些观点以及术后恶心呕吐的风险因素。虽然以往大量的文献报道了如何预防和治疗术后恶心呕吐,但迄今为止,5-HT3受体拮抗剂仍然是临床上使用的最主要的止吐药。目前,一些新药物,如NK-1受体拮抗剂、更加长效的5-HT3受体拮抗剂以及对于PONV高风险的病人实行的多模式管理和新技术的应用,正变得越来越突出。本文综述了现今手术后恶心呕吐的防治方法的最新进展。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.