楼梯草属四新种和一新变种

描述了荨麻科楼梯草属小叶楼梯草组一新变种,维西楼梯草,骤尖楼梯草组骤尖楼梯草系一新种,兜船楼梯草,南川楼梯草系二新种,罗氏楼梯草和异晶楼梯草,以及疏毛楼梯草系一新种,福贡楼梯草。     

中国楼梯草属和赤车属(荨麻科)植物在各省的分布新记录

报道荨麻科楼梯草属和赤车属共18种植物在我国9个省区的分布新记录,其中有安徽的对叶楼梯草,福建的骤尖楼梯草和异被赤车。甘肃的庐山楼梯草。广西的大叶赤车,贵州的樱叶楼梯草,裂序楼梯草,曲毛赤车和波缘赤车,湖南的梨序楼梯草,四川的裂序楼梯草和漾濞楼梯草,西藏的翅苞楼梯草,拟盘托楼梯草,贡山楼梯草和李恒楼梯草,云南的短齿楼梯草,无苞楼梯草,樱叶楼梯草。裂序楼梯草和伏毛楼梯草。     

云南东南部楼梯草属二新种

描述了从云南东南部发现的荨麻科楼梯草属二新种,其中黄连山楼梯草隶属于骤尖楼梯草组骤尖楼梯草系,腺点楼梯草隶属于骤尖楼梯草组盘托楼梯草系.     

云南东南部楼梯草属二新种

描述了从云南东南部发现的荨麻科楼梯草属二新种,其中黄连山楼梯草隶属于骤尖楼梯草组骤尖楼梯草系,腺点楼梯草隶属于骤尖楼梯草组盘托楼梯草系。     

中国楼梯草属(荨麻科)几种植物的订正

根据对中国楼梯草属Elatostema植物标本的研究,将碧江楼梯草E．bijiangense归并入楼梯草E． involucratum,长叶墨脱楼梯草E．medogense var．oblongum和树志楼梯草E．shuzhii归并入墨脱楼梯草E． medogense,光茎钝叶楼梯草E．obtusum var．glabrescens归并入三齿钝叶楼梯草E．obtusum var．trilobula- tum,三裂楼梯草E．sinense var．trilobatum归并入对叶楼梯草E．sinense,赤水楼梯草E．strigulosum var．semitripilinerve归并入伏毛楼梯草E．strigulosum。     

广西楼梯草属八新种

描述了自广西发现的荨麻科楼梯草属八新种,少花楼梯草,隆安楼梯草,粗柱头毛楼梯草,罗城楼梯草,糙茎楼梯草,三列苞楼梯草,基序楼梯草和黄果楼梯草,并分别给出其与近缘种的区别特征。     

楼梯草属研究随记

在该文中首次给出革叶楼梯草的雄头状花序描述; 还给出樱叶楼梯草雄头状花序的修正描述和兜苞墨脱楼梯草的修正特征集要; 写出托叶楼梯草和南川楼梯草二种的分类学修订,其中包括2新变种和2新等级; 描述了小叶楼梯草组的1新种和骤尖楼梯草组的3新种; 数年前被归并为异名的兜苞墨脱楼梯草和五肋楼梯草得到恢复。     

楼梯草属研究随记

在该文中首次给出革叶楼梯草的雄头状花序描述;还给出樱叶楼梯草雄头状花序的修正描述和兜苞墨脱楼梯草的修正特征集要;写出托叶楼梯草和南川楼梯草二种的分类学修订,其中包括2新变种和2新等级;描述了小叶楼梯草组的1新种和骤尖楼梯草组的3新种;数年前被归并为异名的兜苞墨脱楼梯草和五肋楼梯草得到恢复。     

中国楼梯草属(荨麻科)植物省级分布新记录

报道荨麻科楼梯草属7种1变种植物在我国6个省区分布的新记录,其中广西分布新记录的有算盘楼梯草和多歧楼梯草,贵州分布新记录的有曲毛楼梯草,海南分布新记录的有锐齿楼梯草和深绿楼梯草,湖南分布新记录的有短毛楼梯草,四川分布新记录的有丝梗楼梯草,云南分布新记录的有细角楼梯草。     

安徽省楼梯草属新记录

报道了产于安徽省楼梯草属2新记录种,分别为锐齿楼梯草(Elatostema cyrtandrifolium)、深绿楼梯草(Elatostema atroviride).     

New Materials of Eriocaulon L. from China

This paper consists of a part of pertinent data obtained through a critical study of Eriocaulaceae from China. Included in it are three new series: Leiantha, Robustiora and Mangshanensia; seven new species: Eriocaulon acutibracteatum, E. angustulum, E. bilobatum, E. leianthum, E. sclerophyllum, E. glabri-petalum and E. mangshanense; five new varieties: E. rockianum var. latifolium, E. merrillii var. longibracteatum, E. sikokianum var. Linanense, E. alpestre var. sichanense and E. nantoense var. micropetalum; two new combinations: E. yaoshanense var. brevicalyx; E. nantoense var. parviceps; three new records in China: E. brownianum, E.brownianum var.nilagirense, E. zollingerianum; five: E. henryanum, E.pullum, E. yaoshanense, E. taishanense and E.faberi. In addition, fifteen taxon names are newly reduced to synonyms: E. yunnanense=E. brownianum; E. longifolium, E. sexangulare var. longifolium, E. sinii, E. kwangtungense and E. willdinovianum = E. sexangulare; E. setaceum var. capillus-naiadis= E. setaceum; E. filifolium = E. yaoshanense; E. suishaense, E. merrillii var. suishaense=E.merrillii; E. kengii=E.sikokianum; E. whangii=E.buergerianum; E. nipponicum, E. decemflorum var. nipponicum= E. decemflorum and E. nantoense var.trisectum = E. nantoense var.parviceps.     

Apolipoprotein E polymorphism in Saudis

Apolipoprotein E (apoE) genotypes were determined in 165 Saudis. The prevalence of genotype, E3/E3, E3/E4 and E4/E4 was found to be 71, 27 and 2% respectively. The E3/E3 was the most prevalent genotype among the Saudis followed by E3/E4. However, other genotypes E2/E2, E2/E3 and E2/E4 were absent showing the absence of E2 allele in the test population. The high frequencies of the E3 allele (0.845) and E3/E3 genotype (0.71) and absence of E2 allele in Saudis under study are similar to those reported earlier for Native Americans, Mexican-Americans, Mayans, Cayapa, Mazatecan Indians and Mexican Mestizos populations.     

中国人群(京津地区)载脂蛋白E基因多态性和表型分布的研究

本文利用作者建立的密度梯度超速离心分离VLDL的新方法和分析等电聚焦电泳,测定了95例中国人Apo-E基因多态性和其表型分布以及血脂水平。所得表型分布趋势与国外报告一致:E_3/E_3频率最高,E_3/E_2和E_3/E_4次之,E_4/E_4,E_4/E_2和E_2/E_2最低。同对发现中国人群的E_3/E_3表型百分分布明显高于西方人群,但未发现有E_2/E_2表型。我国人群Apo-E表型分布的这种特征可能与中国人群冠心病的患病率较西方人群为低有关。血清脂质测定和分析表明,具有不同表型人群的血脂水平无显著的统计学差异。     

Apolipoprotein E polymorphism in Saudis

Apolipoprotein E (apoE) genotypes were determined in 165 Saudis. The prevalence of genotype, E3/E3, E3/E4 and E4/E4 was found to be 71, 27 and 2% respectively. The E3/E3 was the most prevalent genotype among the Saudis followed by E3/E4. However, other genotypes E2/E2, E2/E3 and E2/E4 were absent showing the absence of E2 allele in the test population. The high frequencies of the E3 allele (0.845) and E3/E3 genotype (0.71) and absence of E2 allele in Saudis under study are similar to those reported earlier for Native Americans, Mexican-Americans, Mayans, Cayapa, Mazatecan Indians and Mexican Mestizos populations.     

New species of Elaphoglossum (Elaphoglossaceae) from northern South America

The fern genusElaphoglossum is well-represented in the Venezuelan pteridoflora with 98 species. Careful observation of the indument of rhizome and blade is necessary to distinguish the taxa. Thirty-three species and one variety are here describe as new:E. anceps, E. appressum, E. atrorubens, E. atrosquamatum, E. chrysopogon, E. crispatum var.crispatum, E. crispatum var.beitelii, E. delicatulum, E. dolichopus, E. drewianum, E. eriopus, E. floccosum, E. grallator, E. hieracioides, E. incubus, E. luteynii, E. maguirei, E. nigrocostatum, E. obovatum, E. ornithoglossum, E. ortegae, E. pilosius, E. praetermissum, E. stenoglossum, E. stergiossi, E. steyermarkii, E. styriacum, E. succubus, E. tachirense, E. tantalinum, E. urophyllum, E. vanderwerffii, E. vareschianum, andE. variolatum.     

Organization of the cores of the mammalian pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and their capacities to bind the E1 and E3 components

The subunits of the dihydrolipoyl acetyltransferase (E2) component of mammalian pyruvate dehydrogenase complex can form a 60-mer via association of the C-terminal I domain of E2 at the vertices of a dodecahedron. Exterior to this inner core structure, E2 has a pyruvate dehydrogenase component (E1)-binding domain followed by two lipoyl domains, all connected by mobile linker regions. The assembled core structure of mammalian pyruvate dehydrogenase complex also includes the dihydrolipoyl dehydrogenase (E3)-binding protein (E3BP) that binds the I domain of E2 by its C-terminal I' domain. E3BP similarly has linker regions connecting an E3-binding domain and a lipoyl domain. The composition of E2.E3BP was thought to be 60 E2 plus approximately 12 E3BP. We have prepared homogenous human components. E2 and E2.E3BP have s(20,w) values of 36 S and 31.8 S, respectively. Equilibrium sedimentation and small angle x-ray scattering studies indicate that E2.E3BP has lower total mass than E2, and small angle x-ray scattering showed that E3 binds to E2.E3BP outside the central dodecahedron. In the presence of saturating levels of E1, E2 bound approximately 60 E1 and maximally sedimented 64.4 +/- 1.5 S faster than E2, whereas E1-saturated E2.E3BP maximally sedimented 49.5 +/- 1.4 S faster than E2.E3BP. Based on the impact on sedimentation rates by bound E1, we estimate fewer E1 (approximately 12) were bound by E2.E3BP than by E2. The findings of a smaller E2.E3BP mass and a lower capacity to bind E1 support the smaller E3BP substituting for E2 subunits rather than adding to the 60-mer. We describe a substitution model in which 12 I' domains of E3BP replace 12 I domains of E2 by forming 6 dimer edges that are symmetrically located in the dodecahedron structure. Twelve E3 dimers were bound per E248.E3BP12 mass, which is consistent with this model.     

DNA-damage response control of E2F7 and E2F8

Here, we report that the two recently identified E2F subunits, E2F7 and E2F8, are induced in cells treated with DNA-damaging agents where they have an important role in dictating the outcome of the DNA-damage response. The DNA-damage-dependent induction coincides with the binding of E2F7 and E2F8 to the promoters of certain E2F-responsive genes, most notably that of the E2F1 gene, in which E2F7 and E2F8 coexist in a DNA-binding complex. As a consequence, E2F7 and E2F8 repress E2F target genes, such as E2F1, and reducing the level of each subunit results in an increase in E2F1 expression and activity. Importantly, depletion of either E2F7 or E2F8 prevents the cell-cycle effects that occur in response to DNA damage. Thus, E2F7 and E2F8 act upstream of E2F1, and influence the ability of cells to undergo a DNA-damage response. E2F7 and E2F8, therefore, underpin the DNA-damage response.     

Experimental hybridizations of Eriophyllum annuals (Asteraceae, Helenieae)

Seven of the eight annual species of Eriophyllum, a western North American genus, were studied in greenhouse and garden. Eriophyllum congdonii and E. nubigenum proved to be self-compatible; E. ambiguum, E. multicaule, E. pringlei, and E. wallacei were self-incompatible; E. lanosum was not studied. All combinations of artificial hybridizations were attempted except E. congdonii × E. lanosum. Among the species with n = 7, fertile hybrids came from E. congdonii × E. nubigenum and sterile hybrids from E. ambiguum var. ambiguum × E. pringlei, E. ambiguum var. paleaceum × E. congdonii, E. multicaule, E. nubigenum, and E. pringlei, as well as E. multicaule × E. congdonii and E. multicaule × E. pringlei. The other homoploid combinations failed, as did heteroploid combinations involving the annual species with n = 7 and either E. wallacei (n = 5) or E. lanosum (n = 4). Sterile hybrids were generated in crosses of E. congdonii to the perennial E. lanatum (x = 8).     

Hybrid pyruvate dehydrogenase complexes reconstituted from components of the complexes from Escherichia coli and Azotobacter vinelandii

The pyruvate dehydrogenase complex of Escherichia coli was isolated in a simple three-step procedure. Its chain stoichiometry, determined by trinitrobenzoate modification was found to be 1.4 E1:1 E2:0.6 E3. It was reproducible within 10% from preparation to preparation. The E. coli complex was resolved by chromatography on activated thiol Sepharose. Reconstitution of activity yielded a stoichiometry of 1.0 E1:1 E2:0.5 E3. The optimum binding stoichiometry of E1E2 and E2E3 subcomplexes was determined by sedimentation experiments and found to be 2.0 E1:1 E2 and 2.5 E3:1 E2, respectively. Competition between E1 and E3 was observed in the binding experiments, but not in the kinetic experiments. Hybrid active complexes could be reconstituted from either an E1E2 subcomplex from Azotobacter vinelandii and the E3 component from E. coli or from E2E3 subcomplex from E. coli and the E1 component from A. vinelandii. Low activity and weak binding was observed when E1 from E. coli was recombined with an E2E3 subcomplex from A. vinelandii or when E3 from A. vinelandii was recombined with an E1E2 subcomplex from E. coli. The association behaviour and stoichiometry of the reconstituted complexes is determined by the nature of the E2 component. The formation of hybrid complexes indicates a considerable structural similarity between the complexes from both sources, despite the differences in size and stoichiometry.     

Identification of enterococci at the species level by sequencing of the genes for D-alanine:D-alanine ligases

A PCR assay based on the use of degenerate oligodeoxyribonucleotides allowed characterization of a fragment internal to the ddl genes encoding D-alanine:D-alanine ligases in Enterococcus columbae, E. durans, E. malodoratus, E. mundtii, E. raffinosus, E. seriolicida, E. solitarius, and E. sulfureus. Phylogenetic analysis of the sequence of the amplification products and of those already obtained from E. aviuni, E. casseliflavus, E. cecorum, E. dispar, E. faecalis, E. faecium, E. flavescens, E. gallinarurm, E. hirae, E. pseudoavium, and E. saccharolvticus yielded an evolutionary tree with a topology similar to that based on 16S rRNA sequences. Partial sequencing of the ddl gene can therefore be used for genotypic identification of Enterococcus spp.