TBP的联结因子TAFs的结构与功能

ＴＢＰ的联结因子ＴＡＦｓ的结构与功能潘建伟赵章杏朱睦元（杭州大学生命科学学院,杭州３１００１２）潘伟槐黄承才（绍兴文理学院生化系,绍兴３１２００）ＳｔｒｕｃｔｕｒｅｓａｎｄＦｕｎｃｔｉｏｎｓｏｆＴＢＰ－ａｓｏｃｉａｔｅｄＦａｃｔｏｒｓＴＡＦｓＰＡＮＪ...     

环境因子对香根草生长习性的影响

环境因子对香根草生长习性的影响夏汉平,敖惠修,何道泉（中国科学院华南植物研究所,广州５１０６５０）ＥｆｆｅｃｔｏｆＥｎｖｉｒｏｎｍｅｎｔａｌＦａｃｔｏｒｓｏｎＶｅｔｉｖｅｒＧｒａｓｓＧｒｏｗｔｈ．￥ＸｉａＨａｎｐｉｎｇ;ＡｏＨｕｉｘｉｕ;ＨｅＤａｏｑ...     

抗σ因子

Ｔ4噬菌体的ＡｓｉＡ可以抑制大肠杆菌的σ70 因子 ,因而称为抗σ70 因子 ,调节Ｔ4噬菌体早期基因的转录。细菌中的ＦｌｇＭ ,是抗σ2 8因子在细菌鞭毛形成过程中起调控作用。ＦｌｇＭ比较特殊 ,天然形式是未折叠的。枯草杆菌(Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ)的ＳｐｏＩＩＡＢ可抑制芽孢形成过程的专一性σ因子σＦ 和σＧ。该菌中的另一个抗σ因子是ＲｓｂＷ可以抑制应急反应中基因转录所需的σＢ 因子。此外 ,在真细菌类中 ,发现一类新的具内膜接合特性的抗σ因子 ,调控着具有胞外功能蛋白的基因表达 ,因而被分作抗σ因子的细胞外因子 …     

三叶因子3的研究

三叶因子 3 (ｔｒｅｆｏｉｌｆａｃｔｏｒ 3 ,ＴＦＦ3 )是一种胃肠道粘膜修复因子 ,1 991年在大鼠ｃＤＮＡ文库中第一次被克隆 ,它由 60个氨基酸组成 ,分子中含有 7个Ｃｙｓ ,其中有 6个Ｃｙｓ以Ｃｙｓ1 Ｃｙｓ5 、Ｃｙｓ2 Ｃｙｓ4、Ｃｙｓ3 Ｃｙｓ6 的形式构成二硫键 ,形成一个状似叶片的紧凑结构 ,因而最初命名为小肠三叶因子 (ｉｎｔｅｓｔｉｎａｌｔｒｅｆｏｉｌｆａｃ ｔｏｒ,ＩＴＦ) [1] 。哺乳动物中有 3种三叶因子 ,分别为含有一个三叶结构域的乳腺癌相关肽(ＴＦＦ1或ｐＳ2 ) ,含有两个三叶结构域的解痉挛多肽 (ＴＦＦ2或ＳＰ)以及…     

早孕因子化学本质的研究进展

早孕因子化学本质的研究进展杨德吉,吉传义,龚晓明（南京农业大学动物医学系,南京２１００９５）关键词早孕因子早孕因子（ＥＰＦ,ＥａｒｌｙＰｒｅｇｎａｎｃｙＦａｃｔｏｒ）是存在于早孕血清中的一种免疫抑制因子。胎儿在母体内不被当作异体抗原而受到免疫排斥,这...     

玉米反式因子基因分离初报

发育是一个严格有序的选择性基因表达过程。在这一过程中,序列特异的反式因子（ｔｒａｎｓａｃｔｉｎｇｆａｃｔｏｒｓ）通过与基因调控序列中顺式调控元件（ｃｉｓａｃｔｉｎｇｅｌｅｍｅｎｔｓ）的互作启动基因转录,因此,它们又被称为转录因子［１］。基于这类因...     

植物基因表达调控的机制：核因子作用

植物基因表达调控的机制：核因子作用邵宏波（四平师范学院生物工程研究所四平１３６０００）１引言真核生物的转录启始是由顺式作用的ＤＮＡ基序（ｅｔｓ－ａｃｔｉｎｇＤＮＡｍｏｔｉｆ）与反式作用的蛋白质因子（ｔｒａｎｓ－ａｃｔｉｎｚｎｒｏｔｅｉｎｆａｃｔｏｒ）...     

桡足类消化酶活力的影响因子

桡足类消化酶活力的影响因子汤鸿,李少菁（厦门大学海洋系,３６１００５）ＦａｃｔｏｒｓＩｎｆｌｕｅｕｃｉｎｇｔｈｅＤｉｇｅｓｔｉｖｅＥｎｚｙｍｅＡｃｔｉｖｉｔｙｏｆＣｏｐｅｐｏｄｓ．￥ＴａｎｇＨｏｎｇ;ＬｉＳｈａｏｃｈｉｎｇ（Ｏ－ｃｅａｎｏｌｏｇｙＤｅ...     

人肿瘤坏死因子基因在变铅青链霉菌中的克隆与表达

以质粒ｐＩＪ４８６为载体,将来源于质粒ｐＨＴ１的肿瘤坏死坏因子（ＴＮＦ－ａ）ｃＤＮＡ克隆至变铅青链霉菌,以新霉素（３０μｇ／ｍｌ）为选择标记,获得了数百转化子,实验表明Ｎｏ．７转化子ｓ．ｌｉｖｉｄａｎｓ ＴＫ５４－ＨＴ所含重组质粒ｐＩＪＴ７已克隆有ＴＮＦｃＤＮＡ。Ｌ９２９细胞毒实验结果表明该转化子ＴＮＦ表达量可达１０＾８活性单位／升以上,中和实验确证其表达产物为人ＴＮＦ－ａ,ＳＤＳ－ＰＡＧＥ表明克     

血管生成因子研究进展

近年来,随着基因重组技术和蛋白质工程技术的完善和发展,一类可促进新生血管生成的蛋白多肽被相继发现,它们被统称为血管生成因子(ａｎｇｉｏｇｅｎｉｃｆａｃｔｏｒ,ＡＦ)[1]。目前已被分离纯化和克隆的有:成纤维细胞生长因子(ｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃ ｔｏｒ,ＦＧＦ)、血管内皮细胞生长因子(ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｇｒｏｗｔｈｆａｃｔｏｒ,ＶＥＧＦ)、血小板衍生生长因子(ｐｌａｔｅｌｅｔｄｅｒｉｖｅｄｇｒｏｗｔｈｆａｃｔｏｒ,ＰＤＧＦ)、转化生长因子(ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏ…     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

鸡传染性法氏囊病病毒研究进展

传染性法氏囊病(Infection bursal disease, IBD)是由鸡传染性法氏囊病毒(Infectious bursal disease virus, IBDV)引起的鸡和火鸡的一种高度接触性传染病,给世界各国的禽养殖业带来了巨大损失.自IBDV发现至今新的变异株不断出现,分子结构的改变导致病毒致病力的改变及宿主对疫苗应答的改变,使得传统的疫苗已不能控制其流行,因此各国学者对其基因组结构和功能进行了广泛深入的研究,并积极研制新型有效的疫苗以达到防治的目的.     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).