MICB基因多态性与乳腺癌的易感关联性研究

目的:研究海南汉族人群MICB等位基因的多态性与乳腺癌易感性之间的关联性。方法:采用PCRSSP(PCR sequence-specific primers)和PCR-SBT(PCR sequence-based typing)方法对样本MICB等位基因的多态性进行检测。结果:乳腺癌患者中检出14种MICB等位基因;和对照组相比较,MICB*002和MICB*014等位基因在乳腺癌患者组分布频率较少,MICB*002和MICB*014等位基因可能对乳腺癌不易感(MICB*002:OR=0.31,95%CI:0.19-0.51,Pc0.05;MICB*014:OR=0.32,95%CI:0.17-0.60,Pc0.05)。MICB*016和MICB*003等位基因在乳腺癌患者组分布较多;MICB*016和MICB*003等位基因可能对乳腺癌易感(MICB*016:OR=10.68,95%CI:2.52-45.28,Pc0.05;MICB*003:OR=3.57,95%CI:1.34-9.49,Pc0.05);MICB*002/002和MICB*014/014基因型可能对乳腺癌不易感(MICB*002/002:OR=0.12,95%CI:0.04-0.36,Pc0.05;MICB*014/014:OR=0.30,95%CI:0.10-0.89,Pc0.05)。结论:MICB等位基因的多态性与乳腺癌的易感性之间存在关联性。     

MICA基因多态性与海南人群HBV易感关联性研究

研究MICA等位基因多态性与海南人群HBV感染易感性之间的关联性。采用PCR-SSP和PCR-SBT方法对样本MICA等位基因的多态性进行检测。HBV感染患者中共检出10种MICA等位基因和5种MICA-STR等位基因,和对照组相比较,MICA*010、MICA-A5等位基因可能对HBV感染易感(MICA*010:OR=3. 88,95%CI:2. 19～6. 85,P=0. 000; MICA-A5:OR=1. 27,95%CI:0. 92～1. 77,P=0. 0068)。MICA*008/045基因型可能对HBV感染不易感,MICA*010/010纯合子基因型可能对HBV易感(MICA*008/045:OR=0. 09,95%CI:0. 01～1. 74,P=0. 0071; MICA*010/010:OR=4. 41,95%CI:1. 26～15. 46,P=0. 0106)。MICA等位基因多态性与海南人群HBV感染的易感性间存在关联性。     

MICA/B基因多态性与血吸虫病相关性研究

本研究采用PCR-SSP与PCR-SBT方法对正常健康对照组与血吸虫病感染组、血吸虫病性重度肝纤维化病人组和轻度肝纤维化病人组中MICA/B基因进行分型,并比较各组基因的多态性。结果在血吸虫感染组与健康对照组中共发现13种MICA等位基因和5种MICA-STR基因型,MICA*012:01(11.58%vs 5.83%)、MI-CA*017(2.11%vs 0.00%)及MICA*027(3.16%vs 0.97%)在对照人群组较血吸虫病人组中分布频率较高,但Pc值显示没有统计学意义(Pc>0.05)。MICA-STR型别分析显示,MICA-STR与血吸虫病易感没有相关性,但MICA*A5基因型的分布频率在重度肝纤维化组显著高于轻度肝纤维化组(45.10%vs 26.92%,Pc<0.05)。在血吸虫病人组中一共检出10种MICB等位基因。在本研究人群中未发现与日本血吸虫感染显著相关的MICB等位基因。同时MICB等位基因多态性在重度纤维化组、轻度纤维化组、以及正常对照组相互之间均无显著的相关性。研究显示在血吸虫病人组中,MICA和MICB具有连锁不平衡,其中单倍型MICB*008-MICA*002:01和MICB*014-MICA*045在血吸虫病人组中显示具有显著的连锁不平衡。     

湖南地区HBV患者MHC-Ⅰ类链相关基因A第五外显子微卫星多态性研究

分别提取湖南汉族人群108例慢性乙肝病毒(hepatitis B virus,HBV)患者、96例HBV携带者和142例健康对照者外周血基因组DNA,利用聚合酶链反应和基因扫描技术分别对它们的主要组织相容性复合体Ⅰ类链相关A(major histocompatibility complex class Ⅰ chain related A,MICA)基因第5外显子进行微卫星多态性分析;应用DNA测序分析对不同的基因型进行验证,同时应用PCR/SSP技术进行MICA*Del检测,确定MICA基因第5外显子基因型.发现本研究的三组中分别检出A4、A5、A5.1、A6、A9五种等位基因,且以A5和A5.1为主;在HBV携带组和健康对照组中分别检出MICA* Del基因.结果同时显示慢性HBV患者组MICA*A5.1/A9基因型频率、慢性HBV患者组的MICA*A9等位基因频率、慢性HBV患者组MICA* A9表型频率和HBV携带组MICA*A5.1/A9基因型频率均低于相应的健康对照组;而湖南地区汉族人群HBV携带者和慢性HBV患者间的基因型频率、等位基因频率和表型频率无显著性差异;因而推测MICA*A5.1/A9基因型和MICA*A9等位基因可能是抗HBV感染的一种保护性等位基因.为今后进一步研究HBV的感染、预防和治疗提供参考依据.     

湖南汉族人群MICB等位基因多态性与白血病的相关性研究

目的:研究湖南汉族人群MICB等位基因的多态性与白血病的相关性。方法:MICB等位基因分型用PCR-SSP和PCR-SBT的方法。结果:白血病患者中有13种MICB等位基因被检测出,其中MICB*005:02/010基因频率较对照组显著偏低(OR=0.416,P<0.05)。不同类别白血病之间MICB等位基因多态性没有显著差异。结论:MICB*005:02/010等位基因可能与白血病的保护相关。     

贵州侗族人群MICB等位基因多态性研究

目的:探讨贵州侗族健康人群MICB等位基因的分布特点。方法:收集100例健康无亲缘关系的贵州侗族人新鲜血液样本,采用世界卫生组织(WHO)推荐的标准盐析法从样本新鲜血液中提取基因组DNA,并用PCR-SSP、PCR-SBT两种方法对样本DNA进行MICB等位基因分型。结果:在贵州侗族人群中,检出7种MICB等位基因,其中MICB*005:02等位基因频率最高,其频率为58.50%,其次为MICB*002:01等位基因频率22.00%;而MICB*003及MICB*005:03等位基因频率最低,两者频率分别为1.50%。结论:贵州侗族人群MICB等位基因具有高度的多态性,该数据为研究MICB基因在同种异体器官移植和疾病易感性中的可能作用奠定了实验基础。     

MICB基因多态性与肺癌的易感关联性研究

目的:研究海南汉族人群MICB等位基因的多态性与肺癌易感性之间的关联性。方法:采用PCR-SSP(PCR sequence-specific primers)和PCR-SBT(PCR sequence-based typing)方法对样本MICB等位基因的多态性进行检测。结果:肺癌患者中检出14种MICB等位基因;和对照组相比较,MICB*00502等位基因在肺癌患者组分布频率较少(43.5%vs 57.8%),MICB*016等位基因在肺癌患者组分布较多(5.9%vs 0.6%);MICB*016等位基因可能对肺癌易感(OR=11.19,95%CI:2.59-48.24,Pc0.05);MICB*00502等位基因可能对肺癌不易感(MICB*00502:OR=0.56,95%CI:0.42-0.76,Pc0.05)。结论:MICB等位基因的多态性与肺癌的易感性之间存在关联性。     

湖南地区HBV患者MHC—I类链相关基因A第五外显子微卫星多态性研究

分别提取湖南汉族人群108例慢性乙肝病毒（hepatitis B virus,HBV）患者、96例HBV携带者和142例健康对照者外周血基因组DNA,利用聚合酶链反应和基因扫描技术分别对它们的主要组织相容性复合体I类链相关A（major histocompatibility complex class I chain related A, MICA）基因第5外显子进行微卫星多态性分析;应用DNA测序分析对不同的基因型进行验证,同时应用PCR／SSP技术进行MICA＊Del检测,确定MICA基因第5外显子基因型。发现本研究的三组中分别检出44、A5、A5．1、A6、A9五种等位基因,且以A5和A5．1为主;在HBV携带组和健康对照组中分别检出MICA＊Del基因。结果同时显示慢性HBV患者组MICA＊A5．1／A9基因型频率、慢性HBV患者组的MICA＊A9等位基因频率、慢性HBV患者组MICA＊A9表型频率和HBV携带组MICA＊A5．I／A9基因型频率均低于相应的健康对照组;而湖南地区汉族人群HBV携带者和慢性HBV患者问的基因型频率、等位基因频率和表型频率无显著性差异;因而推测MICA＊A5．1／A9基因型和MICA$A9等位基因可能是抗HBV感染的一种保护性等位基因。为今后进一步研究HBV的感染、预防和治疗提供参考依据。     

乙醇代谢酶基因多态性与酒精性肝病关系

目的:研究乙醇代谢酶基因ADH3、ALDH2及CYP2E1 RsaⅠ/PstⅠ位点多态性与酒精性肝病(ALD)的关系。探讨遗传因素对其易感性的影响。方法:PCR-RFLP法检测东北地区汉族男性健康者、嗜酒者及ALD患者中ADH3、ALDH2和CYP2E1的基因型并比较其基因频率。结果:ADH3和CYP2E1的两种等位基因在各组中的分布差异无统计学意义;ALDH2的两种等位基因在三组间的频率存在统计学差异,ALDH2~*1在嗜酒及ALD组中的频率显著高于其在对照组中的频率,二组均以ALDH2~*1/2~*1基因型为主,未见ALDH2~*2/2~*2;与嗜酒者相比,ALDH2~*2在ALD组中的频率显著提高,且均为ALDH2~*1/2~*2。结论:ADH3及CYP2E1 RsaⅠ/PstⅠ位点多态性与东北地区汉族男性ALD的发生无关;ALDH2~*1是嗜酒的诱因,ALDH2~*2/2~*2可防止嗜酒及ALD的发生,ALDH2~*1/2~*2具有最高的患病风险。     

缓激肽B2受体基因多态性对骨关节炎易感性和严重性的影响

目的:研究缓激肽B2受体(BDKRB2)基因多态性对骨关节炎的易感性和严重性的影响.方法:在325名原发性膝关节骨关节炎患者及318名健康志愿者中,针对缓激肽B2受体基因多态性-58T/C和+9/-9bp,分别进行基因型测定.结果:+9/-9bp基因多态性的基因型分布和等位基因频率,在骨关节炎组与对照组之间存在明显差异.回归分析显示-9/-9基因型相对于+9/+9基因型,罹患膝关节骨关节炎的风险明显增高(OR=2.354,P＜0.001).同时,+9/-9bp基因多态性与骨关节炎的放射学分型存在相关性,-9bp等位基因可能与骨关节炎的严重程度有关.-58T/C基因多态性与骨关节炎的易感性和严重性无相关性.结论:缓激肽B2受体基因多态性+9/-9bp可能成为检测骨关节炎易感性和严重性的基因标记物.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.