滇姜花中的新二萜成分-滇姜花戊素

从滇姜花根茎中分离到１个新二萜成分,命名为滇姜花戊素（１）,其结构经波谱学方法鉴定为１３β－ｆｕｒａｎｏｌａｂｄａ－８（１７）,１１－ｄｉｅｎ－６β,７α－ｄｉｏｌ。     

滇黄芩中五个新的黄酮类配糖体

从滇黄芩（Ｓｃｕｔｅｌｌａｒｉａ ａｍｏｅｎａ）根中分离到５个新的黄酮类配糖体。命名为滇黄芩甙甲－戊（ａｍｏｅｎｉｎｓＡ－Ｅ）。经光谱分析,化学结构分别证明为：２’,４’,６’－三羟基二氢查耳酮４’－「Ｏ－β－Ｄ－半乳吡喃糖基（１→２）」－Ｏ－β－Ｄ－葡萄吡喃糖甙,５,７,２’－三羟基－６－甲氧基二氢黄酮７－Ｏ－β－Ｄ－葡萄吡喃糖醛酸甙,（顺式）－５,７,２’－三羟基二氢黄酮醇３－Ｏ－β－Ｄ－葡萄     

滇姜花抗肿瘤活性二萜及其光敏氧化反应的研究

从滇姜花（ＨｅｄｙｃｈｉｕｍｙｕｎｎａｎｅｎｓｅＧａｇｎｅｐ．）根茎中分离到两个具抗肿瘤活性的二萜成分滇姜花素Ｃ及滇姜花素Ａ,前者为新化合物,其结构经波谱及化学方法鉴定。此新化合物亦可从滇姜花素Ａ的光敏氧化反应产物中分离得到。     

滇姜花的倍半萜成分

从滇姜花根的乙醇提取物中分离到５个倍半萜,分别是：柳杉醇（１）,α－桉醇（２）、β－桉醇（３）、β－榄醇（４）和４－Ｏ－乙基－柳杉醇（５）。其结构由光谱方法推定,其中（５）为新化合物。     

滇重楼地上部分的两个微量皂甙

从滇重楼地上部分中分离出两个新的微量的甾体皂甙ＰｏｌｙｐｈｙｌｌｏｓｉｄｅⅢ和Ⅳ,根据化学降解和光谱分析,它们的化学结构分别为２７－羟基－偏诺皂皂甙元－３－Ｏ［ａ－Ｌ－鼠李吡喃糖基（１→２）］［ａ－Ｌ－鼠李吡喃糖基（１→４－ａ－Ｌ鼠李吡喃糖基（１→４）］－β－Ｄ－葡萄吡喃糖甙,２３β,２７－二羟基－偏皂甙元－３－Ｏ－［ａ－鼠李吡喃糖（１→２）］     

滇重楼地上部分的配糖体

从滇重楼ＰａｒｉｓｐｏｌｙｐｈｙｌｌａＳｍ．ｖａｒ．ｙｕｎｎａｎｅｎｓｉｓ（Ｆｒ．）Ｈ－Ｍ,地上部分,分离出４个微量的配糖体,经光谱分析和化学降解证明其化学结构分别为２５Ｓ－异钮替皂甙元－３－Ｏ－α－Ｌ－鼠李吡喃糖基（１→２）［α－Ｌ－鼠李吡喃糖基（１→４）］－β－Ｄ－葡萄吡喃甙（Ａ）,２６－β－Ｄ－葡萄吡喃糖基－纽替皂甙元－３－Ｏ－α－Ｌ－鼠李吡喃糖基（１→２）［α－Ｌ－鼠李吡喃糖基（１→４）－β－Ｄ－葡萄吡喃糖甙（Ｂ）,山奈酚－３－Ｏ－β－Ｄ－葡萄吡喃糖基（１→６）－β－Ｄ－葡萄吡喃甙（Ｃ）,７－Ｏ－α－Ｌ－鼠李吡喃糖基－山奈酚－３－Ｏ－β－Ｄ－葡萄吡喃糖基（１→６）－β－Ｄ－葡萄糖甙（Ｄ）。     

滇姜花二萜成分的抗肿瘤活性研究

对滇姜花粗提物、滇姜花素A(1)和姜花酮(2)进行了动物体内抗肿瘤活性测试,结果表明它们均能显著性抑制小鼠体内移植性肿瘤H22的生长,其中滇姜花素A对小鼠H22肿瘤生长抑制率达54.27%,作用最强。体外抗肿瘤实验发现,滇姜花素C(3)对体外培养的人类乳腺癌细胞株MDA-MB-231具有较强的细胞毒作用,并具有明显的剂量效应关系。     

滇产圆滑番荔枝中的番荔枝内酯

从滇南产的圆滑番荔枝的种子中得到４个番荔枝内酯,４－ｄｅｘｏｘｙｃｈｅｒｉｍｏｌｉｎ－２（１）,ｃｈｅｒｉｍｏｌｉｎ－２（２）,ａｎｎｏｎｉｎＩ（３）和ｄｅｓａｃｅｔｙｌｕｖａｒｉｃｉｎ（４）。其中１为新化合物。     

滇金丝猴驯养的初步研究

本文报道的６只慎金丝猴均捕自同一野生种群。由于在运动途中采用了降低应激反应药物,动物得以安全运出。笼养期间,试投以各种本地产植物的叶、花、果和蔬菜为食,结果有５６种为动物所接受。说明金丝猴的食性狭窄主要是受栖息地的限制。在以这些食物为主食的条件下,分别笼养了１４个月的３只（♀）、６个月２只（♂）和８０个月１只（♀）的滇金丝猴目前健康状况良好：成年人体的体重多有所增加,幼体的生长发育良好,结核菌素试     

滇金丝猴血液学和血液生物化学的研究

捕自云南维西,德钦县的６只滇金丝猴（Ｒｈｉｎｏｐｉｔｈｅｃｕｓ ｂｉｅｔｉ）,其中雌性４只,雄性２只,在昆明饲养了６－１２个月,饲料以新鲜树叶和水果为主。结核菌素试验阴性,细菌培养无致病菌生长。于早晨喂食前自上肢取血,用ＴｅｃｈｎｉｃｏｎＲＡ－１００型全自动血液生化分析仪测定了１４个血液生化值和血象。结果发现,尿素氮、肌酐、谷草转氨酶、乳酸脱氢酶、谷氨酰转肽酶比人和猕猴正常参数高,尿酸、甘油三脂     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.