中药903Ⅱ号对由抗生素引起菌群失调患者肠道白色念珠菌的影响

本实验对临床上由抗生素引起菌群失调患者用中药９０３Ⅱ号进行了治疗,在治疗前后分别检测患者肠道白色念珠菌的数量,结果发现菌群失调患者肠道内白色念珠菌数量明显增高,服药后菌群失调得到调整,白色念珠菌数量显著下降。休外实验证明,中药９０３Ⅱ号对白色念珠菌无抑制和杀灭作用。本实验提示,中药９０３Ⅱ号能通过扶植肠道正常菌群间接影响肠道内白色念珠菌。     

中药扶正口服液对肠道正常菌群影响的研究

临床上过量应用抗生素破坏机体内的正常微生物群（normdricrobota）造成微生态失调（nd－CredysbeS）已是公认的事实。近十年来,我们对中药扶正口服液进行了研究,结果证明中药扶正口服液能调整菌群失调,是理想的微生态调节剂。1材料与方法1．1材料1．1．1动物纯系BALBc／J‘鼠体重ZO～229,雌雄各半,白求恩医科大学实验动物中』已提供。1．1．2药物①盐酸林可霉素,浓度为300ndml;②丽珠肠乐,浓度为O．2亿／rnl含菌量;③中药扶正口服液（主成分为人参、当归、猪芬、甘草等）,1号为纯中药复方制剂,中药浓度为3gllnl;11号为中药…     

中药903Ⅱ号对抗生素引起的菌群失调患者肠道菌群的影响

本实验对一些长期应用抗生素而造成菌群失调的病人,服用中药903Ⅱ号进行调整,结果表明长期应用抗生素可引起菌群失调,而中药903Ⅱ号对此有一定调节作用。     

微生态调节剂──中药903口服液药理作用的初步研究

９０３口服液是由中药组成的复方制剂。经药效学实验表明,该制剂具有较好的扶植正常微生物群,调整微生态平衡,生物拮抗的作用。为了进一步考察该制剂对其它系统的影响,我们用中药９０３口服液对小鼠神经系统、心血管系统、呼吸系统、急性毒性试险做了研究。结果表明,中药９０３口服液对其均无显著性影响,且ＬＤ５０为１３７．３±５．８ｇ／ｋｇ,说明该制剂无毒无副作用,是一安全有效的生态调整制剂。     

中药903口服液长期毒性实验研究

中药９０３口服液经药效学研究,表明该制剂具有良好的扶植正常菌群,调整微生态平衡,提高机体免疫能力的作用。对中药９０３口服液进行长期毒性实验研究结果证实,三种不同剂量组大白鼠给药前后的体重,血常规,主要脏器重量,肝、肾功能均无显著性差异。对４０例大鼠１１种脏器病理检查均未见药物损伤性病变。     

微生态调节剂──中药903口服液的稳定性研究

微生态调节剂──中药９０３口服液的稳定性研究佳木斯医学院药剂教研室佳木斯１５４００２于敏佳木斯医学院分析化学教研室霍继明佳木斯医学院微生态研究室杨景云,李丽秋,马淑霞为使中药９０３口服液能大批量生产确定药物有效期和质量标准,我们对其进行了初步稳定性试...     

中药903口服液作为微生态调节剂系列研究进展

中药９０３口服液作为微生态调节剂系列研究进展佳木斯医学院微生态学研究协作组佳木斯１５４００２杨景云,于敏,李丽秋,霍继明,李俭,迟振富,马淑霞,卓越,颜玉当前临床由于大量应用抗生素、激素、免疫抑制剂、细胞毒性药物、同位素及手术等诊治手段,大多对人体内...     

纳米山药多糖合生元结肠靶向微生态调节剂对 菌群失调动物免疫学指标的影响

摘要：目的 研究纳米山药多糖合生元结肠靶向微生态调节剂对抗生素脱污染菌群失调白兔的免疫学效应和机制。方法 用盐酸林可霉素建立菌群失调白兔模型,不同时间点取样,检测各组实验动物胸腺、脾脏指数,采用ELISA法,对各组实验动物肠黏膜sIgA、血清IL-2、IL-6进行检测。结果 各组指标检测结果,制剂组优于阳性对照组,差异有统计学意义（P<0.01）。结论 纳米山药多糖合生元结肠靶向微生态调节剂对抗生素脱污染菌群失调白兔免疫学指标有显著影响。     

中药903对抗生素脱污染小鼠肠道菌群的影响

本实验用抗生素给小鼠肠道脱污染造成肠菌群失调后.用中药903进行调整,旨在用中药903通过扶持肠道内有益菌群,抑制不利菌群,达到恢复微生态平衡的目的。将昆明种小鼠随机分成7组,每组6只小鼠,第1组为正常对照组,喂以与药物同体积的水,其他6组为抗生素脱污染组,联合用硫酸新霉素(20mg/ml)、头孢菌素V钠(20mg/ml)给小鼠灌胃,每天2次,每次0.5ml,连续3天。将6组抗生素脱污染组再分为5组治疗组,和1组抗生素脱污染对照组即自然恢复组。5组治疗组是:中药9031号     

微生态学与后抗生素时代

本文简述了抗生素产生的历史背景,和对人类感染性疾病作出的贡献。但由于抗生素的泛用、滥用和过度治疗,引起了机体的菌群失调、二重感染和更为严重的细菌耐药性。为解决抗生素的弊端,解决菌群失调等问题,促使了微生态学的兴起和发展。本文还阐述了细菌耐药性的现状及危害,细菌产生耐药性的动因,后抗生素时代对细菌的防控措施。提出用微生态制剂、肠道正常菌群、中药、抗菌肽、噬菌体、研制新型抗菌药和规范用药等措施,防控细菌感染。同时阐述了常用中药的抗菌谱,及与微生态制剂合并应用的可能性。     

液相BANA试验检测口腔关键厌氧菌方法的建立

目的建立液相苯甲酰精氨酸萘酰胺(简写BANA)水解试验快速检测牙周病关键厌氧菌的新技术。方法利用牙周病相关关键微生物可产生胰蛋白酶样酶使人工合成的酶底物———BANA水解的原理检测这些重要口腔厌氧菌。结果BANA试验可检测出8.0×105以上的牙龈卟啉菌,特异性良好、不受标本采集中血污染的影响;71.88%活动期牙周炎龈下菌斑BANA试验阳性,4%的健康人龈下菌斑BANA试验阳性。结论BANA试验可望成为牙周病诊断新的辅助指标。     

Screening for in vitro metabolites of Abelmoschus manihot extract in intestinal bacteria by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Abelmoschus manihot has drawn much attention recently due to its potential beneficial health effects after oral administration. However, the metabolic fate of A. manihot in intestinal flora is not well understood. In this paper, we describe a strategy using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) with automated data analysis software (MetaboLynx?) for fast analysis of the metabolic profile of flavonoids from A. manihot in intestinal flora. The human and rat incubated samples collected 72 h in the anaerobic incubator were analyzed by UPLC-Q-TOF MS within 10 min. A total of 14 metabolites were identified in human and rat incubated solution compared with blank samples. The results indicated that hydrolysis, hydroxylation and acetylation were the major metabolic pathways of flavonoids in A. manihot extract in vitro. MS(E) was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the fast structural characterization of metabolites. This work demonstrated the potential of the UPLC-Q-TOF MS approach using Metabolynx for fast and automated identification of metabolites of natural product in intestinal flora.     

Metabolism of nitropolycyclic aromatic hydrocarbons by human intestinal microflora

Anaerobic bacterial suspensions from human and rat feces and intestinal contents, and pure cultures of anaerobic bacteria metabolized 1-nitropyrene and 6-nitrobenzo[a]pyrene to 1-aminopyrene and 6-aminobenzo[a]pyrene, respectively. The metabolites were isolated by reversed-phase high performance liquid chromatography and identified by comparison of their chromatographic and mass spectral properties with those of authentic compounds. The results suggest that anaerobic intestinal bacteria could play a significant role in the metabolism of potentially carcinogenic nitropolycyclic aromatic hydrocarbons.     

Anaerobic Metabolism of 1-Amino-2-Naphthol-Based Azo Dyes (Sudan Dyes) by Human Intestinal Microflora

The rates of metabolism of Sudan I and II and Para Red by human intestinal microflora were high compared to those of Sudan III and IV under anaerobic conditions. Metabolites of the dyes were identified as aniline, 2,4-dimethylaniline, o-toluidine, and 4-nitroaniline through high-performance liquid chromatography and liquid chromatography electrospray ionization tandem mass spectrometry analyses. These data indicate that human intestinal bacteria are able to reduce Sudan dyes to form potentially carcinogenic aromatic amines.     

草鱼肠道黏膜厌氧细菌的分离与鉴定

研究以草鱼(Ctenopharyngodon idellus)为实验对象, 运用厌氧培养的方法, 研究了饥饿状态下草鱼肠道黏膜固有微生物的类群及其在不同肠段的分布。实验结果显示草鱼前肠、中肠与后肠中细菌的数量分别是3.17×103、1.63×104和1.79×107 cfu/g。研究共分离到274株单菌落, 经16S rRNA鉴定, 分别属于拟杆菌属(Bacteroides spp.)、鲸杆菌属(Cetobacterium spp.)、梭形杆菌属(Fusobacterium spp.)、气单胞菌属(Aeromonas spp.)、希瓦氏菌属(Shewanella spp.)、芽孢杆菌属(Bacillus spp.)、泛菌属(Pantoea spp.)和柠檬酸杆菌属(Citrobacter spp.)8个种类, 其中专性厌氧细菌的数量占9.1%, 兼性厌氧细菌的数量占90.9%。进一步分析发现, 前肠中只分离到兼性厌氧细菌, 中肠与后肠专性厌氧细菌和兼性厌氧细菌都有分布。专性厌氧细菌Bacteroides paurosaccharolyticus和Bacteroides luti在中肠与后肠都有分布, 而Cetobacterium somerae和Fusobacterium ulcerans只在后肠有发现。兼性厌氧细菌是草鱼肠道黏膜的优势菌群, 其中嗜水气单胞菌Aeromonas hydrophila占73.7%。草鱼肠道不同部位固有厌氧微生物组成存在差异, 细菌数量也明显不同, 后肠中具有更高的细菌丰度和多样性。     

四种厌氧菌生化性状检测方法的比较

使用国内的三种厌氧菌生化鉴定装置及购进的API20A 条,对标准参考菌株10 株,临床分离株109 株及从粪便分离株 14 株共计 133 株进行了测试比较,虽然这四种方法各有其优缺点,但结果这几种方法与国外的API20A 法相比,其符合率及重复率均无明显差异(P> 0.05),而国内的厌氧菌生化鉴定装置都具有快速,简便、准确的特点,无论是符合率还是重复率都在80% 或90% 以上,当然这四种方法还存在着一定问题待改进,所以我们希望上述国内有关装置在不断地改进中完善,使我们的厌氧菌检测水平更上一层楼。     

鲤科（Cyprinidate）鱼肠道菌群分析

对江苏大纵湖区的草鱼、白鲢、团头鲂和鲤鱼等4种鱼肠道菌群中的好氧菌或兼性厌氧菌进行分离鉴定.对每种鱼的肠道中菌群数量和种类组成进行了分析,发现在上述鱼肠道中存在哈夫尼亚菌属、致病杆菌属、气单胞菌属、柠檬酸菌属、假单胞菌属、链球菌属、葡萄球菌属等.     

饥饿胁迫对克氏原螯虾生理生化和肠道健康的影响

研究旨在探究饥饿胁迫对克氏原螯虾(Procambarus clarkii)血淋巴生理生化指标、肠道组织病理和肠道微生态的影响.对克氏原螯虾进行为期28d的饥饿胁迫,分别于饥饿0、7d、14d和28d采集血淋巴和肠道样品.血淋巴生化分析结果表明,谷丙转氨酶(ALT)没有发生显著变化,血糖(GLU)水平随着饥饿时间延长逐渐...     

Anaerobic oral flora in the North American black bear (Ursus americanus) in eastern North Carolina

Microbial flora can provide insight into the ecology and natural history of wildlife in addition to improving understanding of health risks. This study examines the anaerobic oral flora of hunter killed black bears (Ursus americanus) in eastern North Carolina. Oral swabs from the buccal and lingual supragingival tooth surfaces of the first and second mandibular and maxillary molars of 22 black bears were inoculated onto Brucella Blood Agar plates supplemented with hemin and vitamin K after transport from the field using reduced oxoid nutrient broth. Sixteen anaerobic bacterial species, representing nine genera were identified using the RapID ANA II Micromethod Kit system and a number of organisms grown that could not be identified with the system. The most frequently identified anaerobes were Peptostreptococcus prevotii, Streptococcus constellatus, and Porphyromonas gingivalis. The diversity in the anaerobic oral flora of black bear in eastern North Carolina suggests the importance of including these organisms in basic health risk assessment protocols and suggests a potential tool for assessment of bear/habitat interactions.     

多器官功能不全综合征时肠道细菌微生态学改变的实验研究

为研究多器官功能不全综合征（ＭＯＤＳ）时肠道细菌微生态的变化以及Ｇ＾－杆菌消长与肠道和血液中内毒素水平的关系,本实验选用ＳＤ大鼠,应用无菌Ｚｙｍｏｓａｎ腹腔注射制备大鼠ＭＯＤＳ模型,并对ＭＯＤＳ大鼠肠道菌群进行定量分析,对门静脉和外周静脉血中的内毒以及肠道游离内毒素含量进行定量测定。结果发现,ＭＯＤＳ状态下肠道细菌微生态发生明显变化,表现为肠杆菌和肠球菌等肠道内需氧菌的数量明显增多,双歧杆菌和乳酸