胸腺神经内分泌细胞的特性和系统演化”

胸腺神经内分泌细胞的特性和系统演化李丕鹏（烟台师范学院生物系暨生物研究所烟台２６４０２５）关键词脊椎动物蛇ＡＰＵＤ细胞神经内分泌微环境基质细胞在对蛇胸腺微环境的研究基础上,应用嗜银和亲银染色、铅苏木精染色、５ＨＴ和ＣＣＫ等１６种激素的免疫组化染色和...     

虎斑颈槽蛇胸腺APUD细胞的免疫组织化学观察

虎斑颈槽蛇胸腺ＡＰＵＤ细胞的免疫组织化学观察ＴＨＥＩＭＭＵＮＯＨＩＳＴＯＣＨＥＭＩＣＡＬＯＢＳＥＲＶＡＴＩＯＮＯＦＴＨＥＴＨＹＭＩＣＡＰＵＤＣＥＬＬＳＩＮＴＨＥＳＮＡＫＥＲＨＡＢＤＯＰＨＩＳＴＩＧＲＩＮＡＫｅｙｗｏｒｄｓＲｈａｄｂｄｏｐｈｉｓｔｉｇｒ...     

LFA—1／ICAM—1在ConA诱导的小鼠胸腺细胞活化中的作用

用抗ＬＦＡ－１／ＩＣＡＭ－１粘附分子单克隆抗体和ＣｏｎＡ联合刺激小鼠胸腺细胞,初步研究了该膜分子在经ＴＣＲ／ＣＤ３介导的胸腺细胞活化信号传导以及胸腺细胞亚群选择中的作用。在ＣｏｎＡ刺激系统中,抗ＡＬＦＡ－１／ＩＣＡＭ－１单抗均能抑制胸腺细胞的增殖应答,且以抗ＬＦＡ－１单抗均能抑制胸腺细胞的增殖应答,且以抗ＬＦＡ－１单抗的作用更为显著,而在ＰＡＭ加钙离子载体Ａ２３１８７刺激体系中,抗ＬＦＡ－１单抗却     

小鼠胸树突状细胞系自发分泌多种类型细胞因子

建成小鼠胸腺基质细胞系，命名为ＭＴＳＣ４，经鉴定分析认为是ＤＣ来源。在无外来刺激条件下，ＭＴＳＣ４细胞可自发分泌多种类型细胞因子，已检测到的有ＩＬ－１，ＩＬ－６，ＩＬ－７，ＣＳＦ，ＩＦＮ及趋化因子（ＣＦ）等。其中ＩＬ－６，ＩＦＮ为较高水平分泌量，ＩＬ－１、ＣＦ为中等水平，ＩＬ－７、ＣＳＦ为较低水平分泌量。ＭＴＳＣ４不能自发分泌ＩＬ－３及ＴＮＦａ。ＭＴＳＣ４的建立有利于分析胸腺选择特别是阴性选择的机     

骨巨细胞瘤M-CSF、IL-1和TNF-α免疫组织化学研究

本实验应用免疫组织化学方法,检测巨噬细胞相关抗原、巨噬细胞集落刺激因子（Ｍ┐ＣＳＦ）、白细胞介素┐１（ＩＬ┐１）和肿瘤坏死因子┐α（ＴＮＦ┐α）在骨巨细胞瘤（ＧＣＴ）中的表达,结果表明,在ＧＣＴ中,Ｍ┐ＣＳＦ主要由纤维母细胞样基质细胞（ＦＣ）分泌;巨噬细胞样基质细胞（ＨＣ）是单核┐巨噬细胞系早期的未成熟细胞,主要分泌ＩＬ┐１和ＴＮＦ┐α,少数多核巨细胞（ＭＧＣ）也可产生ＴＮＦ┐α。本文提示,ＧＣＴ中的三种主要细胞能分泌Ｍ┐ＣＳＦ、ＩＬ┐１和ＴＮＦ┐α细胞因子,通过自分泌或旁分泌作用相互影响,共同促进ＧＣＴ的发生、发展及其侵袭性的生物学行为     

二甲亚砜与维甲酸对人卵巢癌细胞COC1的生长抑制及转化生长因子β_1表达的影响

本实验以人卵巢癌细胞株（ＣＯＣ１）为模型,观察诱导分化剂二甲亚砜（ＤＭＳＯ）与维甲酸（ＲＡ）对该细胞生长增殖、ＤＮＡ合成和转化生长因子β１（ＴＧＦβ１）在细胞内表达的影响。结果显示：ＤＭＳＯ与ＲＡ对人卵巢细胞ＣＯＣ１生长有明显的抑制作用,生长曲线表明作用５天后其生长抑制率分别为６２．７％和４２．１％;３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入实验说明ＤＭＳＯ组与ＲＡ组的单位时间计数率（ＣＰＭ）明显低于对照组（Ｐ＜０．０１）。用药３天后百分掺入抑制率分别为６０．４％与３７．９％,表明ＤＭＳＯ与ＲＡ抑制ＣＯＣ１细胞的ＤＮＡ合成;免疫细胞化学反应表明,ＤＭＳＯ或ＲＡ处理５天后,对照组细胞ＴＧＦβ１表达为阳性,定位于胞浆,而处理组细胞ＴＧＦβ１呈阴性或弱阳性反应。以上结果提示ＤＭＳＯ和ＲＡ对人卵巢癌细胞有一定的诱导分化作用。     

cAMP与小鼠胸腺细胞凋亡相关性的研究

本文采用Ｆｏｒｓｋｏｌｉｎ诱导小鼠胸腺细胞凋亡,探讨胞内ｃＡＭＰ浓度上升与细胞凋亡的相关性,采用透射电镜,ＦＣＭ和ＭＴＴ法,结果显示：Ｆｏｒｓｋｏｌｉｎ能诱导小鼠胸腺细胞凋亡,不同浓度其作用程度不同,已由ＦＣＭ证实,透射电镜显示细胞凋亡的形态学特征,Ｆｏｒｓｋｏｌｉｎ激活胞内腺苷酸环化酶,产生大量ｃＡＭＰ,其诱导细胞凋亡数目最多的浓度,ＭＴＴ法显示其光密度（ＯＤ５７０）值最高,表示细胞活性最强。我     

黄脊蛇肺微血管的扫描电镜观察

黄脊蛇肺微血管的扫描电镜观察ＥＭＯＢＳＥＲＶＡＴＩＯＮＯＮＴＨＥＬＵＮＧＭＩＣＲＯＶＡＳＣＵＬＡＴＵＲＥＯＦＴＨＥＳＮＡＫＥＣＯＬＵＢＥＲＳＰＩＮＡＬＩＳ关键词黄脊蛇肺微血管构筑扫描电镜ＫｅｙｗｏｒｄｓＣｏｌｕｂｅｒｓｐｉｎａｌｉｓ,Ｌｕｎｇ,Ｍｉｃ...     

十种蛇喉部形态的比较

十种蛇喉部形态的比较ＡＣＯＭＰＡＲＡＴＩＶＥＳＴＵＤＹＯＮＴＨＥＬＡＲＹＮＧＥＳＯＦＴＥＮＳＰＥＣＩＥＳＯＦＳＮＡＫＥＳ关键词蛇，喉，形态，解剖ＫｅｙｗｏｒｄｓＳｎａｋｅ，Ｌａｒｙｎｘ，Ｍｏｒｐｈｏｌｏｇｙ，Ｃｏｍｐａｒａｔｉｖｅａｎａｔｏｍｙ中图分...     

云南蛇菰属─新种

云南蛇菰属─新种钱义咏（云南省思茅行署林业局,云南思茅６６５０００）关键词蛇菰属;澜沧蛇菰;新种澜沧蛇菰新种图１ＡＮＥＷＳＰＥＣＩＥＳＯＦＢＡＬＡＮＯＰＨＯＲＡＦＲＯＭＹＵＮＮＡＮ￥ＱｉａｎＹｉｙｏｎｇ（ＦｏｒｅｓｔｒｙＢｕｒｅａｕｏｆＳｉｍａｏＡｄ...     

Antibiotics in the treatment of wounds in the victims of the earthquake in the Armenian SSR

Purulent wounds in 88 victims of the Armenian earthquake with the syndrome of long-term squeezing were examined microbiologically and the experience with using antimicrobial drugs for their treatment was analyzed. In all the cases microbial associations were detected, coliform and nonfermenting gram-negative bacilli, enterococci, anaerobes and yeast-like fungi being isolated from 80, 20, 15 and 25 per cent of the patients respectively. 130 bacterial strains were studied in detail. 80 to 100 per cent of the bacterial isolates were resistant to the antibiotics routinely used in surgical practice, i.e. benzylpenicillin, tetracycline, levomycetin, kanamycin, cephalothin and cefazolin and highly sensitive to new antimicrobial drugs belonging to ureidopenicillins (mezlocillin), quinolones (ciprofloxacin) and cephalosporins of the 3rd generation (cefoperazone and ceftazidime). Retrospective estimation of the antibiotic therapy revealed its inadequacy in 55.7 per cent of the patients for the most part because of the isolates resistance. The 19 variants of the antibiotic use prescribed by the physicians mainly included penicillins, aminoglycosides and their combinations (in 67.2 per cent of the cases). The use of the highly efficient drugs of the groups of ureidopenicillins and quinolones was extremely rare which was likely due to lacking of information on the drugs.     

Changes in the size of the nucleoli of the Purkinje cells in the canine cerebellum in the postresuscitation period

The changes in the size of Purkinje cell (PC) nucleolus in the lateral and medial cerebellum zones were studied in dogs with different degree of neurologic status recovery after clinical death of various etiology and duration. PC always possess one nucleolus in the control and experimental groups. In the case of complete neurologic status recovery of animals the area of PC nucleolus increases in both zones studied, irrespective of the cause of clinical death. In the case of neurologic disorders the increase in PC nucleolus area is clearly expressed only in the medial zone of the cerebellum, being insignificant in the lateral zone. It is suggested that adaptive characteristics of PC are distinct in the two compared zones, which leads to greater PC vulnerability in the lateral zone during deep hypoxia.     

On the role of the pineal in thermoregulation in the pigeon

Diurnal rhythm in body temperature of pigeons subjected to different experimental conditions (pinealectomized, sham-operated, melatonin-implanted, cold-exposed) was studied under a 12 h light and 12 h dark regimen. The body temperature of pigeons during photophase was higher than that during scotophase in the normal as well as every treatment group studied. Pinealectomized pigeons showed higher body temperature in the photophase as well as scotophase, than that of the normal and sham-operated birds when examined 2 or 3 weeks following the post-surgical acclimatization to 25 or 3 degrees C. However, subcutaneous implantation of melatonin pellets into pinealectomized pigeons nullified or even reversed the hyperthermic effect of pinealectomy. Exposure of pigeons to--18 degrees C for 280 min during photophase as well as scotophase, produced a marked drop in body temperature in pinealectomized, sham-operated and normal pigeons. The pinealectomized pigeons exhibited a higher body temperature than that of the sham-operated and normal ones when exposed to--18 degrees C during the photophase, but not during the scotophase. It was concluded that while the pineal is not necessary for maintaining the daily thermal rhythm in the avian body, it has a thermoregulatory role, in that it prevents rise in body temperature in warm (25 degrees C) acclimatized and chronic cold (3 degrees C) exposed birds. In acute short-term cold (--18 degrees C) exposure however, the temperature regulatory role of the pineal was not effective during the scotophase.     

Histogenesis of the taste buds of the vallate papilla in the in the rat in the postnatal stages of development

The developing taste buds of vallate papillae were studied with electron microscope in rats during the first 7 days after birth. Two types of cells--light and dark--are identified in the taste buds of a one day old animal. The apical parts of dark cells are characterized by numerous dark granules. A distinguishing feature of light cells is the presence of synaptic contacts with afferent intragemmal nerves. On the 4th day of development on the top of the apical parts of the cell, a microvillar apparatus is seen to form, which does not yet communicate with the oral cavity. On the 7th day, basal cells appear in the taste buds. Some of these cells are seen mitotically dividing. The differentiated microvillar apparatus now communicates with oral cavity. The structure of the taste buds is getting similar to that in the adults. The structural and functional peculiarities of the developing taste buds are discussed in association with the period of ontogenesis under consideration.     

Frequency of the stages in the cycle of the seminiferous epithelium in the rat

This study determined the optimum number of tubules to be counted per testis cross section, and the number of animals per treatment group, when changes in stage frequencies in the cycle of the seminiferous epithelium are criteria for assessing effects of treatment on spermatogenesis. A data base of 9,672 observed and staged tubules was collected from testicular cross sections of 15 Sprague-Dawley rats. A significant variation between animals was found for the frequencies of Stages I, II, IV, VI, VIII, and XIII. Computer simulation was used to randomly select different combinations of animal and tubule numbers from the observed data. Stage frequency means from each simulation experiment were compared statistically to observed mean frequencies. A model that used data from all 14 stages was analyzed. The following conclusions were made: a) a minimum of 200 tubule cross sections/testis is recommended for estimating stage frequencies; b) for a fixed number of tubules scored, the number of animals sampled is more important than the number of tubules per animal in reducing variance; c) to detect a difference of 2 standard deviations from the mean with a 2% error rate and examining 200 tubules/testis, at least 12 animals must be used per group when assessing all 14 stages; d) when individual stages are examined using 10 animals per group, only Stage VII has 80% or greater power of test (alpha = 0.05) to detect a frequency difference; e) pooling stages into 3-4 groups is recommended to improve the power of detecting a treatment difference.