海洋真菌Fusariumsp.LD8岩藻多糖酶的液态发酵条件研究

通过对海洋真菌Fusariumsp.LD8产岩藻多糖酶发酵培养基组成及工艺条件的研究,得到较优培养基配方为:麸皮5%,海带粉2%,(NH4)2SO40.8%。以人工海水代替蒸馏水,接种量为3%,在恒温振荡器中以180r/min的速度振荡培养,岩藻多糖酶活可达2.10IU/mL。     

海洋真菌Fusarium sp.LD8岩藻多糖酶的液态发酵条件研究

通过对海洋真菌Fusarium sp.LD8产岩藻多糖酶发酵培养基组成及工艺条件的研究,得到较优培养基配方为:麸皮5%,海带粉2%,(NH4)2SO40.8%。以人工海水代替蒸馏水,接种量为3%,在恒温振荡器中以180r/min的速度振荡培养,岩藻多糖酶活可达2.10IU/mL。     

毛蕊铁线莲的组织培养与植株再生

1植物名称毛蕊铁线莲（Clematis lasiandra Maxim．）,别名小木通、丝瓜花。2材料类别带芽茎段、节间和叶片。3培养条件诱导培养基：（1）MS＋6-BA0．5mg．L-1（单位下同）＋NAA0．05＋3％蔗糖;（2）MS＋6-BA0．5＋NAA0．1＋2,4-D0．1＋3％蔗糖;（3）MS＋6-BA2．O＋NAA0．1＋3％蔗糖。增殖分化培养基：（4）MS＋6-BA1．0＋NAA0．1＋3％蔗糖;（5）MS＋6．BA2．0＋NAA0．1＋2,4-D0．01＋3％蔗糖;（6）MS＋6．BA2．0＋NAA0．05＋3％蔗糖。生根培养基：（7）1／4MS＋NAA0．5＋0．1％活性炭＋15％蔗糖。所有培养基均附加0．6％琼脂粉,pH5．8-6．0,培养温度为（25±2）℃,光照强度为3040gm01．m-2．S-1,光照时间为14h．d-1。     

浪心竹芋的离体培养和快速繁殖

1植物名称 浪心竹芋（Calathea rufibarba vat．Wavestar）,又称浪星竹芋、波浪竹芋。 2材料类别 新生侧芽。 3培养条件 启动培养基：（1）MS＋6-BA5．0mg．L^-1（单位下同）＋NAA0．05＋0．5％卡拉胶＋3％白砂糖;增殖继代培养基：（2）MS＋6-BA3．5＋KT2．0＋0．5％卡拉胶＋3％白砂糖;（3）1／2MS＋6-BA0．4＋KT0．2＋NAA0．05＋0．6％卡拉胶＋4％白砂糖;生根培养基：（4）1／2MS＋NAA0．3＋0．6％卡拉胶＋4％白砂糖。     

旅人蕉的离体快速繁殖

1植物名称旅人蕉（Ravenala madagascariensis Adans．）。 2材料类别顶芽。 3培养条件基本培养基为MS。（1）启动培养基：MS＋6-BA5．0mg.L^-1（单位下同）＋5％椰子乳（CW）＋10％活性炭（AC）;（2）芽增殖培养基：MS＋6-BA4．0＋1．0％AC;（3）壮苗培养基：MS＋6-BA1．0＋10％CW＋1．0％AC;     

千年健的组织培养和快速繁殖

1植物名称千年健（Homalomenaocculta）,别名翡翠宝石。2材料类别茎段。3培养条件基本培养基为MS。（1）诱导不定芽培养基：MS＋6－BA2．0mg·L-1（单位下同）＋NAA0．5;（2）芽增殖培养基：MS＋6－BA1．5＋NAA0．1;（3）生根培养基：1／2MS＋IBA0．5＋NAA0．5。附加3．0％蔗糖（生根培养基2．0％）,0．65％琼脂,pH5．8～6．0。培养温度28～30℃,光照10～12h·d－1,光照度1500～2000lx。4生长与分化情况4．1不定芽的诱导取生长健壮的植株,剥去叶片、叶柄,留下茎段,冲洗干净后用75％酒精消毒20S,然后放在0．回％的Hg…     

大花飞燕草的组织培养及植株再生

1植物名称大花飞燕草（Delphiniumgrandiflorum）,商品名“美丽飞燕草太平洋混色”。2材料类别无菌种子苗真叶。3培养条件基本培养基为MS。（1）种子萌发培养基：1／2MS＋6－BA0．sing·L（单位下同）＋NAA0．2;（2）愈伤组织诱导培养基：MS十6－BAZ5十NAA0．5十IAA0．2;（S）芽分化培养基：MS＋6－BA3＋NAA0．3;（4）生根培养基：MS＋IBA0．5。培养基（1）、（2）中添加3％蔗糖、0．3％琼脂,培养基（3）、（4）中添加3％蔗糖、0．8％琼脂,PHS．8。培养温度25士loC,光照14h·d‘,光照度1500lX。4生长与分化情况4．1…     

岩生肥皂草的组织培养与快速繁殖

1植物名称 岩生肥皂草（Saponaria ocymoides L．）。2材料类别茎尖和节间茎段。3培养条件 以MS为基本培养基,另加3％蔗糖和0．7％琼脂粉,pH5．85。分化、增殖培养基为：（1）MS＋6-BA0．5mg．L-1（单位下同）＋NAA0．1;（2）MS＋6-BA1．0＋NAA0．1;（3）MS＋6-BA0．5＋NAA0-2：（4）MS＋6-BA1．0＋NAA0．2。生根培养基为：（5）MS＋IBA0．2;（6）MS＋IBA0．4。培养温度（25＋2）℃;     

盐爪爪的组织培养与快速繁殖

1植物名称盐爪爪[Kalidium foliatum（Pall．）Moq．]。别名：着叶盐爪爪、灰碱柴。2材料类别无菌苗幼嫩茎段。3培养条件（1）愈伤组织诱导培养基：MS＋6．BA2．0mg．L-1（单位下同）＋2,4．D3．0;（2）不定芽诱导培养基：MS＋6-BA2．0＋NAA0．5;（3）继代增殖培养基：MS＋6-BA1．0＋NAA0．5＋GA0．5：（4）生根培养基：1／2MS＋IBA0．2。上述培养基均添加3％蔗糖和0．7％琼脂,pH5．8—6．0。     

尾巨桉胚培养与快速繁殖研究(简报)

尾巨桉种胚在改良H＋2,4-D0．5mg／L＋6-BA0．1mg／L＋蔗糖3％培养基上诱导形成愈伤组织,每个愈伤组织块在改良H＋6-BA1．0mg／L＋NAA0．5mg／L＋蔗糖5％培养基上分化形成芽点,经30d继代培养,产生有效苗30～50株。利用改良MS＋6-BA0．4mg／L＋NAA0．2mg／L＋蔗糖3％培养基进行壮苗,改良MS＋ABT0．6mg／L＋IBA0．2mg,L＋蔗糖1．5％培养基进行生根诱导．有效成苗率达95％以上。移栽成活率达98％．     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.