| 海洋真菌Fusariumsp.LD8岩藻多糖酶的液态发酵条件研究 |
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| 引用本文： | 吴茜茜,吴克,张洁,谭建新,潘仁瑞,蔡敬民.海洋真菌Fusariumsp.LD8岩藻多糖酶的液态发酵条件研究[J].菌物学报,2006,25(1):77-82. |
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| 作者姓名： | 吴茜茜 吴克 张洁 谭建新 潘仁瑞 蔡敬民 |
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| 作者单位： | 合肥学院生物与环境工程系,合肥,230022 |
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| 基金项目： | 国家留学回国人员科研启动基金;安徽省优秀青年科技基金;安徽省自然科学基金;安徽省教育厅青年教师基金;合肥学院校科研和教改项目 |
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| 摘 要： | 通过对海洋真菌Fusariumsp.LD8产岩藻多糖酶发酵培养基组成及工艺条件的研究,得到较优培养基配方为:麸皮5%,海带粉2%,(NH4)2SO40.8%。以人工海水代替蒸馏水,接种量为3%,在恒温振荡器中以180r/min的速度振荡培养,岩藻多糖酶活可达2.10IU/mL。
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| 关 键 词： | 培养基配方 振荡培养 接种量 酶学性质 |
| 文章编号： | 1672-6472(2006)01-0077-0082 |
Liquid state fermentation of fucoidanase from marine fungi Fusarium sp. LD8 |
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| Authors: | WU Qian-Qian WU Ke ZHANG Jie TAN Jian-Xin PAN Ren-Rui CAI Jing-Min |
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| Abstract: | The media components and cultivation conditions for fucoidanase production from Fusarium sp. LD8 were studied. The optimal fermentation nutrient components were as follow: wheat bran 5%, (NH4)2SO4 0.8% and laminaria powder 2%. The optimal inoculum was 3%. In the case, Fusarium sp. LD8 was cultured at 28 ℃ for 60 hours on a rotary shaker with a speed of 180 rpm, fucoidanase activity reached 2.10 IU/mL. The fucoidanase properties of LD8 were also studied. |
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| Keywords: | fermentation nutrient component optimal inoculum shake culture enzymatic properties |
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