樟科(Lauraceae)部分属种雌蕊群维管分布格局及其系统学意义

运用石蜡切片技术,对樟科的紫楠（Ｐｈｏｅｂｅ ｓｈｅａｒｅｒｉ（Ｈｅｍｓｌ．）Ｇａｍｂｌｅ）,浙江楠（Ｐｈ．ｃｈｅｋｉａｎｇｅｎｓｉｓ Ｓ．Ｂ．Ｓｈａｎｇ）香樟（Ｃｉｎｎａｍｏｍｕｍ ｃａｍｐｈｏｒａ （Ｌｉｎｎ．）Ｐｒｅｓｌ．）浙江桂（Ｃ．ｃｈｅｋｉａｎｇｅｎｓｉｓ Ｎａｋａｉ）,细叶香桂（Ｃ．ｓｕｂａｖｅｎｉｕｍＭｉｑ．）、江浙山胡椒（Ｌｉｎｄｅｒａ ｃｈｉｅｎｉｉ Ｃｈｅｎｇ）、钓樟（Ｌ．ｒ     

中国兰科植物新资料

本文报道了２个新种（元阳石豆兰ＢｕｌｂｏｐｈｙｌｌｕｍｙｕａｎｙａｎｇｅｎｓｅＴｓｕｉ,长帽隔距兰ＣｌｅｉｓｏｓｔｏｍａｌｏｎｇｉｏｐｅｒｃｕｌａｔｕｍＴｓｉ）、２个新组合（翼萼卷瓣兰ＢｕｌｂｏｐｈｙｌｌｕｍｒｅｔｕｓｉｕｓｃｕｌｕｍＲｃｈｂ．ｆ．ｖａｒ．ｏｒｅｏｇｅｎｅｓ（Ｗ．Ｗ．Ｓｍｉｔｈ）Ｔｓｉ,角萼卷瓣兰Ｂ．ｒｅｔｕｓｉｕｓｃｕｌｕｍＲｃｈｂ．ｆ．ｖａｒ．ｔｉｇｒｉｄｕｍ（Ｈａｎｃｅ）Ｔｓｉ）和１个新命名（细茎毛兰ＥｒｉａｔｅｎｕｉｃａｕｌｉｓＳ．Ｃ．ＣｈｅｎｅｔＴｓｉ）。     

中国木兰科统计

中国木兰科统计中国木兰科（Ｍａｇｎｏｌｉａｃｅａｅ）截至１９９３年１２月的资料统计已达１１属１３８种１亚种５变种１变型。其中种类最多的是含笑属（Ｍｉｃｈｅｌｉａ）已发表５８种．其次是木兰属（Ｍａｇｎｏｌｉａ）３４种,木莲属（Ｍａｎｇｌｉｅｔｉａ）３３...     

集约高产过程中土壤有机质动态初探

集约高产过程中土壤有机质动态初探崔玉亭,韩纯儒（北京农业大学,１０００９４）ＤｙｎａｍｉｃｓｏｆＳｏｉｌＯｒｇａｎｉｃＭａｔｔｅｒ（ＳＯＭ）ｉｎｔｈｅＰｒｏｃｅｓｓｏｆＡｇｒｉｃｕｌｔｕｒａｌＩｎｔｅｎｓｉｆｉｃａｔｉｏｎ￥．ＣｕｉＹｕｔｉｎｇ;ＨａｎＣｈｕｎｒｕ（ＢｅｉｊｉｎｇＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ,１０００９４）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（２）：３７—３８．Ｉｎｔｈｉｓｐａｐｅｒ,ｔｈｅｂａｌａｎｃｅｏｆｓｏｉｌｏｒｇａｎｉｃｍａｔｔｅｒ（ＳＯＭ）ｉｎｔｈｅｐｒｏｃｅｓｓｏｆａｇｒｉｃｕｌｔｕｒａｌｉｎｔｅｎｓｉｆｉｃａｔｉｏｎｉｎＣｈａｎｇｚｈｏｕｒｅｇｉｏｎｉｓｓｔｕｄｉｅｄａｎｄａｍｅｄｉｕｍ－ａｎｄｌｏｎｇ－ｔｅｒｍｐｒｅｄｉｃｔｉｏｎｏｆｉｔｓｄｙｎａｍｉｃｓｉｓｍａｄｅ。Ｔｈｅｒｅ－ｓｕｌｔｓｓｈｏｗｔｈａｔｄｕｒｉｎｇｔｈｉｓｐｒｏｃｅｓｓ,ｔｈｅｂａｌａｎｃｅｏｆＳＯＭｉｓｇｅｔｔｉｎｇｂｅｔｔｅｒ,ａｎｄａｆｔｅｒａｍｅｄｉｕｍｏｒｌｏｎｇｐｅ－ｒｉｏｄ（ｅ．ｇ,１０—２０ｙｅａｔｓ）,ｔｈｅＳＯＭｃｏｎｔｅｎｔｃａｎｇｅｔｔ     

用ELISA法测定抗人心肌肌球蛋白轻链(CMLC)单克隆抗体的K_D值

人心肌肌球蛋白由轻链（ｃａｒｄｉａｃｍｙｏｓｉｎｌｉｇｈｔｃｈａｉｎｓ,ＣＭＬＣ）和重链（ｃａｒｄｉａｃｍｙｏｓｉｎｈｅａｖｙｃｈａｉｎｓ,ＣＭＨＣ）组成．当心肌细胞遭到破坏时,ＣＭＬＣ释放入血,可致血中ＣＭＬＣ浓度增高．血中ＣＭＬＣ的测定是一种高度...     

中国木兰科各属次生木质部的解释特征及系统演化

综述了中国木兰科１０属的次生木质部解剖学特征,包括导管分子,纤维管胞和木射线。同时,进一步讨论了其系统演化。这１０属分为两亚科,即木兰亚科（Ｍａｇｍｏｌｉｏｄａｅ）,包括木兰族（Ｍａｇｎｏｌｉｅａｅ）和含笑族（Ｍｉｃｈｅｌｉｅａｅ Ｌａｗ）,木兰族有木莲属（Ｍａｇｌｉｅｔｉａ Ｂｌ．）、华兰木属（Ｍａｎｇｌｉｅｔｉａｓｔｒｕｍ Ｌａｗ）、木兰属（Ｍａｇｎｏｌｉａ Ｌ．）、拟草性木兰属（Ｐａｒａｋｍ     

pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用

观察ｐｐ６０ｃ－ｓｒｃ在血管紧张素Ⅱ（ＡｎｇⅡ）诱导血管平滑肌细胞（ＶＳＭＣｓ）内丝裂原活化蛋白激酶（ＭＡＰＫ）激活中的作用,以了解ＡｎｇⅡ促ＶＳＭＣｓ增殖的信号转导过程。将合成的反义ｃ－ｓｒｃ寡脱氧核苷酸（ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅ－ｏｔｉｄｅｓ,ＯＤＮｓ）以脂质体包裹转染培养的大鼠ＶＳＭＣｓ,用Ｗｅｓｔｅｒｎ印迹测得细胞裂解液中ｐｐ６０ｃ－ｓｒｃ含量明显下降,免疫沉淀方法测得ｐｐ６０ｃ－ｓ     

Dynamic Changes of β Tubulin during the Resumption of Meiosis of Mouse Oocyte

ＤｙｎａｍｉｃＣｈａｎｇｅｓｏｆβＴｕｂｕｌｉｎｄｕｒｉｎｇｔｈｅＲｅｓｕｍｐｔｉｏｎｏｆＭｅｉｏｓｉｓｏｆＭｏｕｓｅＯｏｃｙｔｅＬＩＵＨｕｉ;（刘辉）ＣＨＥＮＤａ－ｙｕａｎ;（陈大元）（ＳｔａｔｅＫｅｙＬａｏｂｏｒａｔｏｒｙｏｆＲｅｐｒｏｄｕｃｔｉ...     

氧化修饰高密度脂蛋白对培养人动脉平滑肌细胞细胞周期蛋白D_1基因表达的影响

动脉平滑肌细胞（ｓｍ ｏｏｔｈ ｍ ｕｓｃｌｅ ｃｅｌｌ,ＳＭＣ）是动脉粥样硬化（ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ,ＡＳ）斑块中的主要细胞,它的增殖在ＡＳ形成过程中极其重要．利用体外培养的人主动脉ＳＭＣ,观察了天然高密度脂蛋白（ｎａｔｉｖｅ ｈｉｇｈ ｄｅｎｓｉｔｙ ｌｉｐｏｐｒｏｔｅｉｎ,Ｎ－ＨＤＬ）及氧化修饰ＨＤＬ（ｏｘｉｄｉｚｅｄ ＨＤＬ,ＯＸ－ＨＤＬ）对培养人主动脉ＳＭＣ ｃｙｃｌｉｎ Ｄ１（细胞周期蛋白Ｄ１）基因转录表达的影响．结果表明：（１）Ｎ－ＨＤＬ对ＳＭＣｃｙｃｌｉｎ Ｄ１基因表达无影响（Ｐ＞ ０．０５）;（２）ＯＸ－ＨＤＬ使ＳＭＣｃｙｃｌｉｎ Ｄ１基因表达显著增强（Ｐ＜０．０１）,其表达量随时间（２、１２、２４ ｈ）延长而增加．上述结果表明,ＯＸ－ＨＤＬ的致ＡＳ作用可能与其刺激ＳＭＣｃｙｃｌｉｎ Ｄ１基因表达增加有关．     

从线粒体细胞色素b探讨长臀？属三个种分类与进化的关系

本研究测定了长臂Ｂａ属（Ｍｙｓｔａｃｏｌｅｕｃｕｓ Ｇｕｅｎｔｈｅｒ）中的３个种：月斑长臂Ｂａ（Ｍ．ｃｈｉｌｏｐｔｅｒｕｓ Ｆｏｗｌｅｒ）、长臂Ｂａ〔Ｍ．ｍａｒｇｉｎａｔｕｓ（Ｃｕｖｒｉｅｒ ｅｔ Ｖａｌｅｎｃｉｅｎｎｓｅ）〕和细尾长臂Ｂａ（Ｍ．ｌｅｐｔｕｒｕｓ Ｈｕａｎｇ）共２２个个体的线粒体细胞色素ｂ部分基因片段,其序列为４０８ｂｐ,并选用Ｐａｎｇ Ｐｉ（Ｒｈｏｄｅｕｓ ｓｉｎｅｎｓｉｓ Ｃｕ     

白蚁与动物及微生物的共生类型及其机制

综述了白蚁螱客的主要种类、共生关系及相关机制的研究进展。白蚁螱客中,已报道的动物种类达170种。在与动物的共生关系中存在偏利共生（宾主共栖和异种共栖）、互利共生和无关共生三种;在与微生物的共生关系中,存在与内生菌（原生动物、细菌、真菌和放线菌）和外生菌（蚁巢伞菌等）间的互利关系。指出了白蚁与螱客研究中存在的问题,给出了解决方案,并提出了今后可能的研究热点或方向,为白蚁的综合利用（如纤维素酶）及今后研究物种间的协同进化提供了基础资料。     

Relative and combined effects of ethanol and protein deficiency on strontium and barium bone content and fecal and urinary excretion

To elucidate accumulation of minerals in human iliac arteries with aging, the content of minerals was analyzed by inductively coupled plasma atomic emission spectrometry. Bilateral common, internal, and external iliac arteries of 16 men and 8 women, ranging ages from 65 to 93 yr, were examined. It was found that an extremely high accumulation of calcium and phosphorus occurred in the common iliac artery at old age, being higher than that of the internal and external iliac arteries. It should be noted that the accumulation of calcium and phosphorus is the highest in the common iliac artery among the human arteries examined to date. Regarding sexual differences, the content of calcium and phosphorus in the common and internal iliac arteries was higher in women than in men, whereas their content in the external iliac artery was lower in women than in men.     

New amino-dithiaphospholanes and phosphoramidodithioites and their rhodium and iridium complexes

New sulfur derivatives of phosphoramidite ligands were synthesized and the impact of the sulfur unit on the spectroscopic properties of their rhodium and iridium complexes was investigated. The new ligands Bn₂NPSCH₂CH₂S^a(P-S^a) (Bn = benzyl, 4), Bn₂NPSCHCHS^a(CH₂)₃C^aH₂(P-S^a)(C^a-S^a) (6) and Bn₂NP(4-XC₆H₄OMe)₂ (X = S, 7a; X = O, 7b) were converted to the rhodium and iridium complexes trans-[Rh(CO)Cl(L)₂] (L = 4, 6, 7), [RhCl(COD)(L)] (L = 4, 6, 7), [IrCl(COD)(7a)] and [IrCl₂Cp∗(6)]. For comparison, some phosphoramidite complexes of these formulations also were synthesized. The new metal complexes were spectroscopically analyzed. For the carbonyl complexes, the ν_CO IR stretching frequencies were lower than for the corresponding phosphite and phosphoramidite ligands. The ¹J_PRh coupling constants for the rhodium complexes with the new ligands were also smaller than for the respective phosphoramidite and phosphite complexes. Finally, the ¹J_PSe coupling constants of the selenides of the new ligands were lower than those of the phosphoramidite ligands but higher than for PPh₃. The spectroscopic data reveal that the new thio ligands 4, 6 and 7a are more electron donating than phosphites and phosphoramidites but less electron donating than PPh₃.     

Astrocytes and Synaptosomes Transport and Metabolize Lactate and Acetate Differently

Astrocytes transport the monocarboxylate acetate, but synaptosomes do not. The reason for this is unknown, because both preparations express monocarboxylate transporters (MCT). The transport and metabolism of lactate, another monocarboxylate, was examined in these two preparations, and the results were compared to those for acetate. Lactate transport is more rapid in astrocytes than in synaptosomes, but of lower affinity (Kms of 17 and 4 mM, respectively). Lactate (0.2 mM) is metabolized to CO2 more rapidly in synaptosomes than in astrocytes (rates of 0.37 and 0.07 nmol x mg protein(-1) x min(-1), respectively). The reason for this is unclear, but cellular differences in lactate dehydrogenase isotype expression may be involved. Acetate is metabolized to CO2 more rapidly in astrocytes than in synaptosomes (rates of 0.43 and 0.02 nmol x mg protein(-1) x min(-1), respectively). This is likely due to cellular differences in the expression of monocarboxylate transporter subtypes.     

Rapporteur report: Basics and technology and metrology and standards

The first and second sessions of the Workshop focussed on the basics of ultrasound and infrasound, their applications in both industry and medicine, and metrology and protection standards for ultrasound applications.     

Prostaglandin and thromboxane production by human and guinea-pig macrophages and leucocytes

The ability of partially purified human and guinea-pig haematogenous cell populations, when cultured in vitro, to metabolise arachidonic acid (AA) has been studied. Supernatants from 24 hour cell culture have been subjected to analysis for products of AA metabolism by gas chromatography with electron-capture detection.The cell types studied were human peripheral blood monocytes (both glass adherent and non-adherent), neutrophils, eosinophils and leukemic leucocytes; thoracic duct lymphocytes and lung alveolar macrophages. From the guinea-pig, induced and non-induced macrophage or neutrophil enriched peritoneal exudate populations, lymph node cells, peritoneal eosinophils and peripheral blood platelets were examined. Supernatants were assayed for the presence of PGE₂, PGD₂, PGF_2α, TXB₂ and 6-keto-PGF_1α. In all types studied PGE₂ and TXB₂ were the major products formed. The identification of PGE₂ and TXB₂ was confirmed by GC/MS with multiple ion monitoring.The results have been compared with other reports and their possible significance discussed in relation to the proposed role of prostaglandins as mediators and modulators in immunopathology.     

人血管能抑素基因的克隆、表达及其表达产物的纯化和生物活性测定

以人胎盘脐带组织为材料,提取组织总RNA,用netRTPCR方法合成人血管能抑素cDNA基因,将该cDNA克隆进pSP72载体获得重组质粒pSP72C, DNA序列分析结果与预期序列一致。用BamHⅠ和NdeⅠ双酶切,切下pSP72C上的血管能抑素cDNA,插入pET3c载体的相应位点获得重组表达质粒pETC, 转化E. coli BL21(DE3), SDSPAGE分析显示：在IPTG诱导下,血管能抑素基因获得了高效表达,表达量约占菌体总蛋白的 27.9 %,主要以包涵体形式存在。包涵体经过洗涤、裂解、蛋白复性以及Sephadex G75凝胶过滤层析等步骤后,获得了纯度达91.4 %的人血管能抑素。CAM实验证明10 μg纯化蛋白就能显著抑制鸡胚新生血管生成。     

Vascular volumes and hematology in male and female runners and cyclists

To examine the hypothesis that foot-strike hemolysis alters vascular volumes and selected hematological properties is trained athletes, we have measured total blood volume (TBV), red cell volume (RCV) and plasma volume (PV) in cyclists (n = 21) and runners (n = 17) and compared them to those of untrained controls (n = 20). TBV (ml x kg(-1)) was calculated as the sum of RCV (ml x kg(-1)) and PV (ml x kg(-1)) obtained using 51Cr and 125I-labelled albumin, respectively. Hematological assessment was carried out using a Coulter counter. Peak aerobic power (VO2peak) was measured during progressive exercise to fatigue using both cycle and treadmill ergometry. RCV was 15% higher (P < 0.05) in male cyclists [35.4 (1.0), mean (SE); n = 12] and runners [35.3 (0.98); n = 9] compared to the controls [30.7 (0.92); n = 12]. Similar differences existed between the female cyclists [28.2 (2.1); n = 9] and runners [28.4 (1.0); n = 8] compared to the untrained controls [24.9 (1.4); n = 8]. For the male athletes, PV was between 19% (cyclists) and 28% (runners) higher (P < 0.05) in the trained athletes compared to the untrained controls. The differences in PV between the female groups were not significant. Although the males had a higher (P < 0.05) TBV, RCV and PV than the females, no differences between cyclists and runners were found for either gender. Mean cell volume was not different between the athletic groups. VO2peak (ml x kg(-1) x min(-1)) was higher (P < 0.05) in both male [68.4 (1.5)] and female [54.8 (2.1)] runners when compared to the untrained males [47.1 (1.0)] and females [40.5 (2.1)]. Although differences existed between the genders in VO2peak for both cyclists and runners, no differences were found between the athletic groups within a gender. Since the vascular volumes were not different between cyclists and runners for either the males or females, foot-strike hemolysis would not appear to have an effect on that parameter. The significant correlations (P < 0.05) found between VO2peak and RCV (r = 0.64 and 0.64) and TBV (r = 0.82 and 0.63) for the males and females, respectively, suggests a role for the vascular system in realizing a high aerobic power.