食用三倍体蕉不定胚的初步观察

食用三倍体蕉不定胚的初步观察陈健辉,杨俊慧,潘坤清（广州师范学院生物系,５１０４００）食用三倍体蕉（Ｍｕｓａ×ｐａｒａｄｉｓｉａｃａ（ＡＢＢｇｒｏｕｐ）．Ｄａｉｉａｏ．Ｍｕｓａ×ａｃｕｍｉｎａｔａ（ＡＡＡｇｒｏｕｐ）,ＤｗｒａｆＣａｖｅｎｄｉｓｈ．）...     

影响 培养基PH值变化的因素

植物组织培养基的ＰＨ值一旦外植体植入就开始发生改变,直到达到某一平衡点,不同种的植物有其各自的ＰＨ平衡点。用６种植物（ＰｉｌｏｔｕｓｅｘａｌｔａｔｕｓＤｅｓｓｅｘＬｅｈｍ,ＬｅｃｈｅｎｎｕｌｔｉａｆｏｍａｓｕｓＲ．ＢＲ．,ＲｏｓａｃａｎｉｎａＬ．,Ｍｅｌａｌｅｕｃａａｌｔｅｒｎｉｆｏｌｉａ（Ｍａｉｄｅｎ＆Ｅ．Ｂｅｔｃｈｅ）Ｃｈｅｅｌ,ＡｎｉｇｏｚａｎｔｈｏｓｆｌａｔｉｄｕｓＤＣ．,Ｚｉｅｒｉａｃｙ     

书评Book Reviews Ⅱ

《中国大型真菌原色图鉴》ＣｏｌｏｒｅｄＩｌｕｓｔａｉｏｎｓｏｆＭａｃｒｏｆｕｎｇｉ（Ｍｕｓｈｒｏｏｍｓ）ｏｆＣｈｉｎａ黄年来主编，林新华责任编辑。１９９８年，中国农业出版社。１－２９３页。书号ＩＳＢＮ－１０９－０４９４１－８／Ｑ．３１６．定价１５０?..     

湖南植物志资料（一）木兰科、八角科、五味子科、樟科、水马齿科补遗

本文报道了湖南植物新记录共计１１种,隶属于５科１０属．归并樟科植物３种２变种：将石木姜子ＬｉｔｓｅａｆａｂｅｒｉＨｅｍｓｌ．和近轮叶木姜子Ｌ．ｓｕｂｖｅｒｔｉｃｉｌｌａｔａＹａｎｇ归入黄丹木姜子Ｌ．ｅｌｏｎｇａｔａ（Ｗａｌｌ．ｅｘＮｅｅｓ）Ｂｅｎｔｈ．ｅｔＨｏｏｋ．ｆ．,将长梗绒楠ＭａｃｈｉｌｕｓｖｅｌｕｔｉｎａＣｈａｍｐ．ｅｘＢｅｎｔｈ．ｖａｒ．ｌｉｎｇｉｐｅｄｕｎｃｕｌａｔａＣ．Ｊ．Ｑｉ归入绒毛润楠Ｍ．ｖｅｌｕｔｉｎａＣｈａｍｐ．ｅｘＢｅｎｔｈ．,将短梗新木姜子ＮｅｏｌｉｔｓｅａｂｒｅｖｉｐｅｓＨ．Ｗ．Ｌｉ归入美丽新木姜子Ｎ．ｐｕｌｃｈｅｌｌａ（Ｍｅｉｓｓｎ）Ｍｅｒｒ．,将紫云山新木姜子Ｎ．ｗｕｓｈａｎｉｃａ（Ｃｈｕｎ）Ｍｅｒｒ．ｖａｒ．ｐｕｂｅｎｓＹａｎｇｅｔＰ．Ｈ．Ｈｕａｎｇ归入巫山新木姜子Ｎ．ｗｕｓｈａｎｉｃａ（Ｃｈｕｎ）Ｍｅｒｒ．．     

关于灌木生物量建模方法的改进

关于灌木生物量建模方法的改进张峰,上官铁梁李素珍（山西大学生物系,太原０３０００６）（太原治金工业学校,０３０００２）ＩｍｐｒｏｖｅｍｅｎｔｏｎｔｈｅＭｏｄｅｌｌｉｎｇＭｅｔｈｏｄｏｆＢｉｏｌｍａｓｓｏｆＢｒｕｓｈ￥ＺｈａｎｇＦｅｎｇ;ＳｈａｎｇｇｕａｎＴｉｅｌｉａｎｇ（Ｄｅｐａｒｔ－ｍｅｎｔｏｆＢｉｏｌｏｇｙ,ＳｈａｎｘｉＵｎｉｖｅｒｓｉｔｙ,Ｔａｉｙｕａｎ０３０００６）,ＬｉＳｕｚｈｅｎ（ＴａｉｙｉｉａｎＭｅｔａｌｌｉｔｒｇｉｃａｌＳｃｈｏｏｌ,Ｔａｉｙｕａｎ０３０００２）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＶＥｃｏｌｏｇｙ,１９９３,１２（６）：６７－６９．ＤｕｒｉｎｇｍａｋｉｎｇｒｅｓｅａｒｃｈｏｎｔｈｅｂｉｏｍａｓｓｏｆＥｌａｅａｇｎｕｓｍｏｌｌｉｓ,ｔｈｅｒｅｇｒｅｓｓｉｏｎｍｏｄｅｌｏｆｔｈｅｂｉｏｍａｓｓｉｓｂｕｉｌｔｂｙｒｅｐｌａｃｉｎｇＤ￣２ＨｗｉｔｈＣＨ,Ｔｈｅｒｅｓｕｌｔｓｈｏｗｓｔｈａｔｎｏｔｏｎｌｙｉｔｉｓｃｏｎｖｉｎｉｅｎｔｉｎｔｈｅｆｉｅｌｄｗｏｒｋ,ｂｕｔａｌｓｏｔｈｅｆｏｒｅｃａｓｔｍｏｄｅｌｈａｓｍｏｒｅＤｒｅｃｉｓｉｏｎｗｈｅｎＣＨｉｓｕｓｅｄａｓｔｈｅｉｎｄｅｐｅｎｄ     

枯草芽孢杆菌（B—912）对桃和油桃褐腐病的抑制效果

研究了不同处理的枯草芽孢杆菌（Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｉｓ（Ｂ－９１２））在不同贮藏温度下对采后桃（Ｐｒｕｎｕｓ ｐｅｒｓｉｃａ Ｌ．）和油桃（Ｐ．ｐｅｒｓｉｃａ ｖａｒ．ｎｅｃｔａｒｉｎａ Ｍａｘｉｍ．）褐腐病（Ｍｏｎｉｌｉｎｉａｆｒｕｃｔｉｃｏｌａ（Ｇ．Ｗｉｎｔ）Ｈｏｎｅｙ）的防治效果。２５℃和１５℃贮藏温度下油桃的发病率一般高于桃,但在冷藏期间其发病率却一般低于桃。１０＾６ＣＦＵ／Ｍ     

古核生物ABC转运体系的重构及其基因组比较

代谢途径和调控网络的重建和比较研究是基因组功能预测的高级内容。运用多种生物信息学工具在基因组水平上重构了古核生物深海热球菌（Ｐｙｒｃｏｃｃｕｓ ａｂｙｓｓｉ）的ＡＢＣ（ＡＴＰ－ｂｉｎｄｉｎｇ ｃａｓｓｅｔｔｅ）转运体系,预测了它们的功能特性;并与另一古核生物詹氏甲烷球菌（Ｍ．ｊａｎｎａｓｃｈｉｉ）的对应体系作了进化比较,发现Ｍ．ｊａｎｎａｓｃｈｉｉ缺少负责短肽摄入的ＡＢＣ转运体系,推测与二者分属不     

细胞松驰素B促微丝解聚对DNA合成的作用

利用微丝（ｍｉｃｒｏｆｉｌａｍｅｎｔ,ＭＦ）解聚药物细胞松驰素Ｂ（ｃｙｔｏｃｈａｌａｓｉｎＢ,ＣＢ）处理Ｇ_０期小鼠Ｃ_３Ｈ_（１０）Ｔ_（１／２）成纤维细胞,对Ｇ_０至Ｓ期ＤＮＡ合成,胸腺嘧啶核苷激酶（ｔｈｙｍｉｄｉｎｅｋｉｎａｓｅ,ＴＫ）活性、ＴＫ基因表达、钙调素（ｃａｌｍｏｄｕｌｉｎ,ＣａＭ）水平和一些细胞周期早期基因的表达进行了观察,Ｇ_０期细胞经３ｍｇ／ＬＣＢ处理２ｈ,促ＭＦ解聚增强了血清对Ｓ期细胞ＴＫ活性、ＴＫ基因表达和ＤＮＡ合成的刺激作用,并促进细胞提前进入Ｓ期．血清刺激Ｇ_０期细胞进入晚Ｇ_１期和Ｓ期时,ＣａＭ水平明显升高,而ＣＢ预处理则使ＣａＭ含量进一步增加,特别是ＣＢ处理促使Ｓ期ＣａＭ增加向核内转移．ＣＢ处理明显增强血清对ｃ－ｊｕｎ、ｃ－ｆｏｓ和ｃ－ｍｙｃ基因表达的刺激作用,而ＰＫＣ抑制剂Ｈ_７则抑制ＣＢ处理对这些基因转录的刺激作用,说明ＣＢ使Ｇ_０期细胞ＭＦ解聚刺激ｃ－ｊｕｎ、ｃ－ｆｏｓ和ｃ－ｍｙｃ的转录活性与ＰＫＣ的作用有关．结果表明Ｇ_０至Ｓ期早期ＭＦ的重组可促进细胞进入Ｓ期,增强ＤＮＡ合成．     

柯萨基B_3病毒对外周血单个核白细胞介素－2受体表达的影响

本文研究了柯萨基Ｂ３病毒（ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３）对正常人ＰＢＭＣ白细胞介素一２受体（ｍＩＬ一２Ｒ）表达的影响,结果实验组为８９．８３±７．０３％,对照组为５２．５±６．１３％,表明ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３能作用于ＰＢＭＣ,使其ｍＩＬ一２Ｒ表达明显减少（Ｐ＜０．０１）,由此影响ＩＬ－２发挥正常的生物学功能,如促使Ｔ细胞增殖,ＮＫ细胞活化等,本文认为ｍＩＬ－２表达减少可能是ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ组病毒所致心肌炎患者细胞免疫功能异常的原因之一。     

地涌金莲化学成分的研究

从地涌金莲（Ｍｕｓｅｌｌａ ｌａｓｉｏｃａｒｐａ（Ｆｒａｎｃｈ．）Ｃ．Ｙ．Ｗｕ．）新鲜植物中分离得到了５种化合物,经理化常数和ＩＲ,ＥＩ－ＭＷ、＾１Ｈ ＮＭＲ,＾１５ＣＮＭＲ波谱数据分析,分别鉴定为,正二十四烷（ｔｅｔｒａｃｏｓａｎｅ,１）,β－谷甾醇（β－ｓｉｔｏｓｔｅｒｏｌ,２）,硬脂酸（ｓｔｅｒａｒｉｅ ａｃｉｄ,３）２,４－二羟基苯甲酸（２,４－ｄｉｔｈｙｄｒｏｘｙ ｂｅｎｚｏｌｅ ａｃｉ     

Investigation of the mechanism of temperature sensitivity of the electroreceptors of ampullae of lorenzini

In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980.     

Determination of stoichiometry of radioiodination of proteins

A sensitive method for the detection of small quantities of hydrophobic antioxidant free radical scavengers such as butylatedhydroxytoluene (BHT) and butylatedhydroxyanisole (BHA) in aqueous samples is described. The procedure involves extraction of the hydrophobic free radical scavenger into an organic solvent phase, followed by the subsequent reaction of an aliquot of this extract with the stable cation radical tris(p-bromophenyl)amminium hexachloroantimonate (TBACA). In experiments with BHT and BHA, the loss of TBACA absorbance at 730 nm was found to be linearly proportional to the amount of antioxidant added, with quantities of BHT as small as 200 pmol being easily detectable. In aqueous suspensions of dimyristoylphosphatidylcholine vesicles, assays of the aqueous BHT concentration showed that BHT partitioned strongly into the membrane phase, achieving very high BHT/phospholipid ratios. For a given concentration of BHT, partitioning into the membrane phase was greater in large, multilamellar liposomes than in either small, single-walled vesicles or in purified rat brain synaptic vesicle membranes. Direct assay of BHT and BHA in phospholipid membranes, however, was complicated by a nonspecific interaction between TBACA and the phospholipid.