排序方式: 共有18条查询结果,搜索用时 109 毫秒
1.
亚麻SRAP反应体系的优化 总被引:1,自引:0,他引:1
通过研究亚麻SRAP反应体系中主要因子对扩增结果的影响,建立了亚麻SRAP-PCR反应的优化体系.在20μL的反应体系中将PCR的5个主要成分分别设定8个浓度梯度,结果表明,最适宜的优化浓度分别为:1.5 mmol/L Mg2+、0.3 mmol/L dNTP、1.5 U Tap酶、30 ng/μL模板DNA 90 ng和25 ng/μL引物100 ng.用6个亚麻材料验证优化体系,检测结果显示,多态性高,反应体系的稳定性和可重复性好,为SRAP标记技术在亚麻分子生物学研究方面的应用奠定了基础. 相似文献
2.
Robert Booy Richard I. Lindley Dominic E. Dwyer Jiehui K. Yin Leon G. Heron Cameron R. M. Moffatt Clayton K. Chiu Alexander E. Rosewell Anna S. Dean Timothy Dobbins David J. Philp Zhanhai Gao C. Raina MacIntyre 《PloS one》2012,7(10)
Background
Influenza is an important cause of morbidity and mortality for frail older people. Whilst the antiviral drug oseltamivir (a neuraminidase inhibitor) is approved for treatment and prophylaxis of influenza during outbreaks, there have been no trials comparing treatment only (T) versus treatment and prophylaxis (T&P) in Aged Care Facilities (ACFs). Our objective was to compare a policy of T versus T&P for influenza outbreaks in ACFs.Methods and Findings
We performed a cluster randomised controlled trial in 16 ACFs, that followed a policy of either “T”—oseltamivir treatment (75 mg twice a day for 5 days)—or “T&P”—treatment and prophylaxis (75 mg once a day for 10 days) for influenza outbreaks over three years, in addition to enhanced surveillance. The primary outcome measure was the attack rate of influenza. Secondary outcomes measures were deaths, hospitalisation, pneumonia and adverse events. Laboratory testing was performed to identify the viral cause of influenza-like illness (ILI) outbreaks. The study period 30 June 2006 to 23 December 2008 included three southern hemisphere winters. During that time, influenza was confirmed as the cause of nine of the 23 ILI outbreaks that occurred amongst the 16 ACFs. The policy of T&P resulted in a significant reduction in the influenza attack rate amongst residents: 93/255 (36%) in residents in T facilities versus 91/397 (23%) in T&P facilities (p = 0.002). We observed a non-significant reduction in staff: 46/216 (21%) in T facilities versus 47/350 (13%) in T&P facilities (p = 0.5). There was a significant reduction in mean duration of outbreaks (T = 24 days, T&P = 11 days, p = 0.04). Deaths, hospitalisations and pneumonia were non-significantly reduced in the T&P allocated facilities. Drug adverse events were common but tolerated.Conclusion
Our trial lacked power but these results provide some support for a policy of “treatment and prophylaxis” with oseltamivir in controlling influenza outbreaks in ACFs.Trail Registration
Australian Clinical Trials Registry ACTRN12606000278538 相似文献3.
Zhanhai?LiEmail author Zhijun?Liu 《In vitro cellular & developmental biology. Plant》2005,41(3):262-265
Summary Camptothecin, produced by Camptotheca acuminata, is a pharmaceutically important compound. Transgenic technology has potential uses for the enhancement of camptothecin production;
however, an efficient plant regeneration protocol for C. acuminata is not currently available. Factors that affected successful seedling germination were evaluated. The regeneration potential
of various parts of seedlings was tested. Camptothecin production in regenerated plants was compared to its production in
calluses and the original seedlings. Dark incubation and seed coat removal led to a higher germination rate and a higher survival
rate after germination. The best shoot induction medium was found to be Gamborg's B5 medium+8.9 μM benzyladenine. Among the calluses induced from various parts of seedlings, leaf petiole calluses, leaf dise calluses, and
cotyledon calluses regenerated shoots, but internode calluses did not. Furthermore, leaf petiole calluses and leaf dise calluses
regenerated normal shoots, while cotyledon calluses regenerated hyperhydric shoots. Moreover, leaf petiole calluses had a
higher shoot regeneration rate, 50% versus 9%, and a higher shoot number, 6.2±0.5 versus 2.0±0.3, than did leaf dise calluses
on the best shoot induction medium. It took 4–6 wk to regenerate shoots after transfer into shoot induction media. Camptothecin
concentration in the regenerated plants was significantly higher than that in the calluses and similar to that in the original
seedlings. In conclusion, leaf petioles provide efficient plant regeneration of C. acuminata. 相似文献
4.
5.
C. Raina MacIntyre Iman Ridda Zhanhai Gao Aye M. Moa Peter B. McIntyre John S. Sullivan Thomas R. Jones Andrew Hayen Richard I. Lindley 《PloS one》2014,9(4)
Background
Elderly people do not mount strong immune responses to vaccines. We compared 23-valent capsular polysaccharide (23vPPV) alone versus 7-valent conjugate (PCV7) vaccine followed by 23vPPV 6 months later in hospitalized elderly.Methods
Participants were randomized to receive 23vPPV or PCV7-23vPPV. Antibodies against serotypes 3, 4, 6A, 6B, 9V, 14, 18C, 19A, 19F, 23F were measured by enzyme-linked immunosorbent (ELISA) and opsonophagocytic (OPA) assays at baseline, 6 months and 12 months.Results
Of 312 recruited, between 40% and 72% of subjects had undetectable OPA titres at baseline. After one dose, PCV7 recipients had significantly higher responses to serotypes 9V (both assays) and 23F (OPA only), and 23vPPV recipients had significantly higher responses to serotype 3 (ELISA), 19F and 19A (OPA only). In subjects with undetectable OPA titres at baseline, a proportionately greater rise in OPA titre (P<0.01) was seen for all serotypes after both vaccines. The GMT ratio of OPA was significantly higher at 12 months in the PCV7-23vPPV group for serotypes 6A, 9V, 18C and 23F. OPA titre levels for these serotypes increased moderately after 6 months, whereas immunity waned in the 23vPPV only arm.Conclusion
We did not show overwhelming benefit of one vaccine over the other. Low baseline immunity does not preclude a robust immune response, reiterating the importance of vaccinating the frail elderly. A schedule of PCV7-23vPPV prevents waning of antibody, suggesting that both vaccines could be useful in the elderly. Follow up studies are needed to determine persistence of immunity.Trial Registration
The Australian Clinical Trials Registry ACTRN12607000387426 相似文献6.
研究分析同属4.1蛋白家族成员merlin-1和ezrin对RhoGDIα抑制pUS28和Dbs诱导SRF激活的调节作用。利用分子生物学、细胞生物学方法和Dual-Luciferase双荧光检测法分析pUS28和Dbs诱导SRF激活,并分析merlin-1(WT)、merlin-1(S519D)、ezrin(WT)和ezrin(T567D)对RhoGDIα抑制pUS28和Dbs诱导SRF激活的调节影响。pUS28和Dbs均可以组成性的诱导SRF因子激活,而RhoGDIα对pUS28和Dbs诱导SRF激活具有显著抑制效应。野生型merlin-1(WT)和磷酸化模拟重组质粒merlin-1(S519D)均可提高RhoGDIα抑制pUS28诱导SRF激活作用,而野生型ezrin(WT)和磷酸化模拟重组质粒ezrin(T567D)均可以降低RhoGDIα的抑制效果。merlin-1(WT)、merlin-1(S519D)对RhoGDIα抑制Dbs诱导的SRF激活没有显著影响或者调节作用。而ezrin(WT)和ezrin(T567D)降低了RhoGDIα在Dbs诱导SRF激活过程中的抑制能力。因... 相似文献
7.
Ying Liu Meng Li Zhanhai Yin Shuangli Zhou Yusheng Qiu 《Cell biology international》2020,44(2):560-568
Articular cartilage damage can lead to joint deformity, pain, and severe dysfunction. However, due to the lack of blood vessels and nerves in articular cartilage, the self‐healing capacity of damaged cartilage is limited. In this study, we overexpressed small ubiquitin‐like modifier (SUMO)1, SUMO2/3, and SUMO1/2/3 in bone marrow mesenchymal stem cells (BMSCs). Then, these cells were inoculated on surfaces of different hardness, and their differentiation into chondrocytes, hypoxic tolerance ability, and inflammatory response was detected. Finally, BMSCs were transplanted into the injured knee joint cavity of the rats, and the repair was evaluated. We found that BMSCs overexpressing SUMO1 were more likely to differentiate into articular cartilage along with the hardness of the surface, while BMSCs overexpressing SUMO2/3 could reduce inflammation response and improve the damaged cartilage microenvironment. In the rat model, BMSCs overexpressing SUMO1/2/3 transplanted on injured articular cartilage surface showed better survival, less inflammatory response, and improved tissue repair capability. In conclusion, BMSCs overexpressing SUMO are more tolerant to hypoxia conditions, and have stronger repair ability for damaged chondrocytes in vitro and for articular cartilage injury model in rats, and are excellent seed cells for repairing articular cartilage. 相似文献
8.
Thenonlinearintegralequation(1.1)isageneralizedmodelforthespreadofdiseaseswithseasonaldependence.Inthispaper,wehaveprovedtheexistenceofatleastthreenontrivialnonnegativeperiodicsolutionstothisequation. 相似文献
9.
10.