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1.
Single Cell Transcriptome Amplification with MALBAC 总被引:1,自引:0,他引:1
2.
Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis 下载免费PDF全文
Salmonella enterica serovar Enteritidis (SE) is a foodborne pathogen that can threaten human health through contaminated poultry products. Live poultry, chicken eggs and meat are primary sources of human salmonellosis. To understand the genetic resistance of egg‐type chickens in response to SE inoculation, global gene expression in the spleen of 20‐week‐old White Leghorn was measured using the Agilent 4 × 44 K chicken microarray at 7 and 14 days following SE inoculation (dpi). Results showed that there were 1363 genes significantly differentially expressed between inoculated and non‐inoculated groups at 7 dpi (I7/N7), of which 682 were up‐regulated and 681 were down‐regulated genes. By contrast, 688 differentially expressed genes were observed at 14 dpi (I14/N14), of which 371 were up‐regulated genes and 317 were down‐regulated genes. There were 33 and 28 immune‐related genes significantly differentially expressed in the comparisons of I7/N7 and I14/N14 respectively. Functional annotation revealed that several Gene Ontology (GO) terms related to immunity were significantly enriched between the inoculated and non‐inoculated groups at 14 dpi but not at 7 dpi, despite a similar number of immune‐related genes identified between I7/N7 and I14/N14. The immune response to SE inoculation changes with different time points following SE inoculation. The complicated interaction between the immune system and metabolism contributes to the immune responses to SE inoculation of egg‐type chickens at 14 dpi at the onset of lay. GC, TNFSF8, CD86, CD274, BLB1 and BLB2 play important roles in response to SE inoculation. The results from this study will deepen the current understanding of the genetic response of the egg‐type chicken to SE inoculation at the onset of egg laying. 相似文献
3.
One new genus of the spider subfamily Coelotinae, Flexicoelotes
gen. n., with five new species is described from southern China: Flexicoelotes
huyunensis
sp. n. (female), Flexicoelotes
jiaohanyanensis
sp. n. (male and female), Flexicoelotes
jinlongyanensis
sp. n. (male and female), Flexicoelotes
pingzhaiensis
sp. n. (female), Flexicoelotes
xingwangensis
sp. n. (male and female). 相似文献
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辽宁省菜田生态系统组分优势值与系统优势度分析 总被引:2,自引:1,他引:1
辽宁省菜田生态系统组分优势值与系统优势度分析周宝利,葛晓光李宁义(沈阳农业大学园艺系,110161)(沈阳市城建中专,110013)AnalysisonSuperiorityValueandDominanceDegreeofVariousCompon... 相似文献
6.
Spectrophotometric quantification of lactic bacteria in alginate and control of cell release with chitosan coating 总被引:3,自引:0,他引:3
Y. Zhou E. Martins A. Groboillot C. P. Champagne & R. J. Neufeld 《Journal of applied microbiology》1998,84(3):342-348
Lactococcus lactis ssp. cremoris was entrapped within a Ca-alginate matrix, and an in situ spectrophotometric method for monitoring cell population in calcium alginate beads described. The intracapsular cell population can be estimated by measuring the optical density of beads containing cells, using cell-free beads as reference, or by measuring absorbance of a liquified bead suspension. Alginate beads, and beads coated with chitosan type I, II, and I and II mixtures, were examined for cell release. Lower viscosity chitosan (type I) coatings reduced cell release by a factor of 100 from105 cfu ml−1 to 103 cfu ml−1 after 6 h of fermentation. Reuse of chitosan I coated alginate beads also showed a reduction in cell release by a factor of 100. Cell loading and initial cell growth within the beads greatly affected cell release. Reducing the initial cell release would lower the overall levels of cell release throughout the fermentation. Compared to non-immobilized cultures, a 20–40% reduction in the lactic acid production rate was observed for alginate beads and chitosan I coated alginate beads, respectively. This reduction can be compensated for by increasing the intracapsular cell loading during immobilization, or before the onset of fermentation. 相似文献
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Norma Diaz-Gustavo Martín Martínez-Salvador José Luís García-Hernández Mariano Norzagaray-Campos Antonio Luna-González Héctor Abelardo González-Ocampo 《PloS one》2015,10(5)
Caesalpinia platyloba was evaluated as an alternative for the retention of atmospheric carbon and as a feasible and viable economic activity in terms of income for tropical deciduous forest (TDF) peasants in the carbon markets. A total of 110 trees of C. platyloba from plantations and a TDF in the Northwest of Mexico were sampled. Growth (increase in height, diameter, and volume curves) was adjusted to assess their growth. Growth of individuals (height, diameter at breast height [DBH], age, and tree crown cover) was recorded. The Schumacher model (H = β0eβ1•E-1), by means of the guided curve method, was used to adjust growth models. Information analysis was made through the non-linear procedure with the multivariate secant or false position (DUD) method using the SAS software. Growth and increase models revealed acceptable adjustments (pseudo R2>0.8). C. platyloba reaches >8m of height with 12cm in diameter and 550cm3 of volume, presenting the highest increase at 11 years considered as basal age. Highest significant density of wood was in good quality sites (0.80g•cm-3), with a carbon content (average of 99.15tC•ha-1) at the highest density of 2500 trees•ha-1 (without thinning). Average incomes of US$483.33tC•ha-1 are expected. The profitability values (NPW = US$81,646.65, IRR = 472%, and B/C = 0.82) for C. platyloba make its cultivation a viable and profitable activity, considering a management scheme of the income derived from wood selling and from carbon credits. 相似文献
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