全文获取类型
收费全文 | 72730篇 |
免费 | 5814篇 |
国内免费 | 4694篇 |
出版年
2023年 | 777篇 |
2022年 | 1044篇 |
2021年 | 3070篇 |
2020年 | 2331篇 |
2019年 | 2917篇 |
2018年 | 3079篇 |
2017年 | 2404篇 |
2016年 | 3273篇 |
2015年 | 4167篇 |
2014年 | 5061篇 |
2013年 | 5502篇 |
2012年 | 6299篇 |
2011年 | 5750篇 |
2010年 | 3937篇 |
2009年 | 3438篇 |
2008年 | 4009篇 |
2007年 | 3574篇 |
2006年 | 3143篇 |
2005年 | 2614篇 |
2004年 | 2351篇 |
2003年 | 2146篇 |
2002年 | 1729篇 |
2001年 | 1461篇 |
2000年 | 1195篇 |
1999年 | 1009篇 |
1998年 | 597篇 |
1997年 | 564篇 |
1996年 | 535篇 |
1995年 | 527篇 |
1994年 | 501篇 |
1993年 | 371篇 |
1992年 | 502篇 |
1991年 | 440篇 |
1990年 | 335篇 |
1989年 | 296篇 |
1988年 | 207篇 |
1987年 | 278篇 |
1986年 | 226篇 |
1985年 | 196篇 |
1984年 | 152篇 |
1983年 | 130篇 |
1982年 | 118篇 |
1981年 | 104篇 |
1980年 | 73篇 |
1979年 | 87篇 |
1978年 | 83篇 |
1977年 | 65篇 |
1975年 | 68篇 |
1973年 | 73篇 |
1972年 | 68篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
1.
2.
András Szilágyi István Scheuring David P. Edwards Jerome Orivel Douglas W. Yu 《Ecology letters》2009,12(12):1306-1316
Theory suggests that spatial structuring should select for intermediate levels of virulence in parasites, but empirical tests are rare and have never been conducted with castration (sterilizing) parasites. To test this theory in a natural landscape, we construct a spatially explicit model of the symbiosis between the ant-plant Cordia nodosa and its two, protecting ant symbionts, Allomerus and Azteca . Allomerus is also a castration parasite, preventing fruiting to increase colony fecundity. Limiting the dispersal of Allomerus and host plant selects for intermediate castration virulence. Increasing the frequency of the mutualist, Azteca , selects for higher castration virulence in Allomerus , because seeds from Azteca -inhabited plants are a public good that Allomerus exploits. These results are consistent with field observations and, to our knowledge, provide the first empirical evidence supporting the hypothesis that spatial structure can reduce castration virulence and the first such evidence in a natural landscape for either mortality or castration virulence. 相似文献
3.
4.
5.
6.
A partial swine cDNA which encodes the functional domain of PIT-1 was isolated by the polymerse chain reaction (PCR). The swine PIT-1 cDNA clone is 95% identical at the protein level to the rat Pit-1 gene. Thus, Pit-l's known function in control of rat growth hormone and prolactin expression is likely to be conserved in swine. This swine cDNA clone was used to investigate genetic variability at PIT-1 in several American and Chinese breeds. Polymorphic BamIII fragments were found in pure-bred Meishan animals (n= 13), but only monomorphic fragments in five American breeds (n= 36). 相似文献
7.
8.
A.N. Reshetilov A.B. Medvinsky T.P. Eliseeva V.Yu. Shakhbazian M.A. Tsyganov A.M. Boronin G.R. Ivanitsky 《FEMS microbiology letters》1992,94(1-2):59-62
A method of pH distribution measurements in agar nutrient media containing expanding bacterial populations is described. It is based on measuring pH microsamples taken at different points of the media. The sample volume was 10 microliters. A pH sensitive field effect transistor was used as a measuring electrode. Acidification was found to occur in glucose media, while alkalization occurred in the media containing peptone. 相似文献
9.
10.
B cell activating factor (BAFF) is a member of the tumor necrosis factor family that is known to play an important role in B cell activation, proliferation, and differentiation in mammals. However, studies of BAFF in teleosts are very limited and its function, in particular that under in vivo conditions, is essentially unknown. In this study, we conducted in vivo as well as in vitro functional analyses of a BAFF homologue (CsBAFF) from the teleost fish tongue sole (Cynoglossus semilaevis). CsBAFF is composed of 261 residues and shares moderate sequence identities with known BAFFs of other teleosts. CsBAFF expression was most abundant in immune organs and was upregulated during bacterial infection. Purified recombinant CsBAFF (rCsBAFF) bound to tongue sole lymphocytes and promoted cellular proliferation and survival. The results of an in vivo study showed that CsBAFF overexpression in tongue sole significantly enhanced macrophage activation and reduced bacterial infection in fish tissues, whereas knockdown of CsBAFF expression resulted in increased bacterial dissemination and colonization in fish tissues. Furthermore, vaccination studies showed that CsBAFF enhanced the immunoprotection of a DNA vaccine and augmented the production of specific serum antibodies. Taken together, these results provide the first in vivo evidence to indicate that teleost BAFF is an immunostimulator that significantly contributes to the innate antibacterial immune response and vaccine-induced adaptive immune response. 相似文献