一种显微图像的拼接方法

描述了一种显微图像拼接的方法,共包括特征检测、特征匹配、空间坐标转换和图像混合等四个步骤。实验结果显示本方法对重叠面积在50％以上的显微图像,能够进行拼接并且都没有出现明显的缝隙、错位、变形、图像模糊、图像重影等错误。     

Governmental policies on HIV infection in China

EVOLUTION OF THE GOVERNMENT POLI- CIES Looking back 20 and more years, one could have hardly imagined that such great changes would have happened to the governmental policies on HIV/AIDS in China. In the earlier phase of the epidemic, laws and regulations were drafted based on the practices and experiences of preven- tion and control of other infectious diseases, such as isolation, quarantine and compulsory testing and examina- tions etc. When the HIV/AIDS epidemic was firstl…     

HIFU治疗下生物体焦域温度分布的研究与应用

高强度聚焦超声(HIFU)为无创治疗肿瘤带来了新的途径,本文对HIFU治疗下生物体焦域热分布和热传导模型的理论进行了初步的介绍,并在理论的指导下进行不同治疗剂量的实验研究,结果表明组织内具有热波传热效应,治疗时可以根据治疗剂量的需要选取不同的参数,以达到最佳治疗效果。     

不同类型磁场对细胞作用的生物学研究

本文就不同参数的磁场的细胞生物学效应研究进行了综述 ,总结了不同类型不同物理参数的磁场对细胞生物学效应的研究成果 ,结合实验结果对磁场生物学效应的可能物理机制进行了初步探讨 ,并对磁场的细胞生物学效应的研究前景进行了展望。     

PBOND： Web Server for the Prediction of Proline and Non-Proline cis / trans Isomerization

PBOND is a web server that predicts the conformation of the peptide bond between any two amino acids. PBOND classifies the peptide bonds into one out of four classes, namely cis imide (cis-Pro), cis amide (cis-nonPro), trans imide (trans-Pro) and trans amide (trans-nonPro). Moreover, for every prediction a reliability index is computed. The underlying structure of the server consists of three stages: (1) feature extraction, (2) feature selection and (3) peptide bond clas- sification. PBOND can handle both s...     

Behavioral modification in choice process of Drosophila

Because of its clear genetic and developmental background, diversity of behavioral paradigms and neuroanatomy of the brain, Drosophila has become an important animal model for studying genetic, molecular and cellular bases of learning and memory[1]. Extensive research has explored the visual operant conditioning of Drosophila and related molecular bases[2—8]; recently, researchers began to address cognition-like functions and involved neural substrates[9—11]. In these studies, behavioral ana…     

基于同源基因的病原菌鉴定和分型靶位点的功能基因组学研究

利用病原菌序列差异,对病原菌特定基因和位点进行检测,可以快速发现和鉴别病原菌的分类和特征,对传染病快速诊断和溯源具有基础性意义和重要价值.本文旨在覆盖中国重要传染病的103种病原菌,寻找各分类阶元中特有的同源基因,并从中挑选出适合用于病原菌鉴定、分型的候选基因.利用生物信息学和基因组学方法,对已有全基因组序列的275株病原菌的836415个基因进行比对分析,进一步明确菌株的门、纲、目、科、属各分类阶元中特有的同源基因集合;通过COG功能分类方法,对同源基因集合进行功能注释,并分析在不同分类阶元内的保守基因功能的变化规律.本研究寻找到适合鉴定和分型的不同分类阶元（门、纲、目、科、属）的同源基因集合共19563个（门2891个、纲1016个、目3601个、科10130个、属1925个）.对同源基因功能的分析表明,适合对病原菌进行鉴定的基因在不同分类阶元中,表现的功能存在较大差异.革兰氏阳性和阴性病原菌在不同分类阶元中,同源基因表现出的功能也存在差异.该结果将为对在中国广泛存在的病原菌进行检测所涉及的探针、芯片设计提供理论依据,加快目标探针的筛选工作.同时,研究也是首次将世界范围内的全基因组数据和中国重大传染病涉及的病原菌紧密联系结合,为利用功能基因组学开展区域性、有针对性的病原检测和监测,提供候选基因和位点筛选的新方法.相关结果在细菌的元基因组学研究中也具有一定的应用价值.     

青藏高原人体脉搏测量及几种脉图对比分析

脉搏信息反映人体内部各器官的健康状况,利用PVDF压力传感器并垫一水囊间接接触法拾取脉搏信息,通过调理放大硬件电路对脉搏信号进行调理放大,借助虚拟仪器软件LabVIEW在PC机上架构虚拟脉搏测试系统,测试并采集了青藏高原地区大量不同人群的脉搏信息数据,给出了几种具有地域特征的脉图,并结合中医脉诊理论进行了对比分析。     

绝缘子调控基因的表达

绝缘子在调控真核基因时空特异表达的过程中起着至关重要的作用.它的主要功能是增强子阻断和异染色质屏障.已经有竞争、阻断和成环等模型描述其增强子阻断功能;而它的异染色质屏障功能主要是通过影响染色质组蛋白的翻译后修饰来实现.已经确定的绝缘子包括果蝇基因组中的染色质特化结构(specialized chromatin structures, scs)和scs、gypsy、鸡珠蛋白β基因座上游的DNaseⅠ高敏感位点cHS4以及小鼠或人Igf2/H19基因座上的印记控制区(imprinting control region, ICR)和DNA甲基化区域(DNA methylated regions, DMR)元件等.许多转录因子参与绝缘子的基因调控作用,例如脊椎动物中的CCCTC结合因子(CCCTC binding factor,CTCF).利用基因组学和生物信息学等方法,还可以在基因组中发现新的绝缘子元件.     

Regulated genes in mesenchymal stem cells and gastric cancer

AIM: To investigate the genes regulated in mesenchymal stem cells(MSCs) and diffuse-type gastric cancer(GC),gene expression was analyzed. METHODS: Gene expression of MSCs and diffuse-type GC cells were analyzed by microarray. Genes related to stem cells, cancer and the epithelial-mesenchymal transition(EMT) were extracted from human gene lists using Gene Ontology and reference information. Gene panels were generated, and messenger RNA gene expression in MSCs and diffuse-type GC cells was analyzed. Cluster analysis was performed using the NCSS software.RESULTS: The gene expression of regulator of G-protein signaling 1(RGS1) was up-regulated in diffuse-type GC cells compared with MSCs. A panel of stem-cell related genes and genes involved in cancer or the EMT were examined. Stem-cell related genes, such as growth arrest-specific 6, musashi RNA-binding protein 2 and hairy and enhancer of split 1(Drosophila), NOTCH family genes and Notch ligands, such as delta-like 1(Drosophila) and Jagged 2, were regulated.CONCLUSION: Expression of RGS1 is up-regulated, and genes related to stem cells and NOTCH signaling are altered in diffuse-type GC compared with MSCs.