Alternations in lipid membrane fluidity and the physical state of cell-surface sialic acid in zinc-deficient rat erythrocyte ghosts

Erythrocyte ghosts, prepared from the blood of rats fed zinc-deficient diets, were evaluated for membrane fluidity and surface sialic acid properties using spin-labeled probes and electron spin resonance (ESR) spectroscopy. These physical parameters of the erythrocyte ghosts from the zinc-deficient group were compared to those for erythrocyte ghosts obtained from ad libitum and pair fed controls consuming zinc-adequate diets. As the animals became progressively zinc deficient, the erythrocyte ghost membranes became more fluid than those from the control groups. In addition, the apparent rotational correlation time of Tempamine spin probes on surface sialic acid residues was smaller for the zinc deficient group, indicative of an increased rotational mobility of the spin label. These results suggest that zinc deficiency can have pronounced effects on the physical state of membrane bilayer lipids and cell surface carbohydrates and supports the view that many of the pathological signs of zinc deficiency are due to a general membrane defect.     

下丘脑外侧区注射TRH对大鼠胃酸分泌的影响

本文采用连续收集胃腔灌流法,观察下丘脑外侧区(LHA)注射促甲状腺激素释放激素(TRH)对大鼠胃酸分泌的影响,并分析TRH在LHA促进胃酸分泌的作用机制。结果表明:(1)LHA注射TRH(1μg)明显地刺激胃酸分泌;(2)预先向LHA注射酚妥拉明(10μg)、美多心安(5μg)及胃泌素抗体1μl(1:640)并不影响TRH的泌酸作用,如预先向LHA注射阿托品(5μg)则可消除TRH的泌酸效应;(3)垂体摘除及肾上腺切除均不影响TRH的泌酸作用;(4)隔下迷走神经切断后,LHA注入TRH的泌酸效应仍然出现,但持续时间显著缩短;腹腔交感神经节摘除后,TRH仍能促进胃酸分泌,但分泌量少而平稳。以上结果提示:LHA是TRH中枢泌酸效应的有关结构之一,其中枢机制是通过胆碱能M受体中介的,腹腔交感神经节和膈下迷走神经是TRH泌酸效应的传出途径。前者引起的泌酸反应出现较早且引起泌酸高峰,但持续时间短;后者则引起低平的持续分泌。     

立枯丝核菌蛋白质电泳图谱研究 Ⅰ.融合群与图谱的相关性

用来自日本和美国的立枯丝核菌8个融合群11个类群代表菌株进行可溶性蛋白质电泳,其结果表明,各融合群及亚群之间电泳图谱有显著差异,而同一类群菌株的电泳图谱则相似。分析来源于华东等地已鉴定的融合类群117个菌株的电泳图谱显示,同一融合群内菌株,虽然采集地区、寄主植物或致病力不同,其蛋白质图谱仍十分相似;而不同融合类群的菌株,即使在同一田块中同一种寄主植物上引起相似病害,其图谱也显示出明显差异。本文就上述可溶性蛋白质图谱显示的结果与其它研究者在血清学、DNA同源性.酯酶等生化水平上对融合群的研究结果进行了比较和探讨。     

Evidence for an increased rate of choline efflux across erythrocyte membranes in Alzheimer's disease

Alzheimer's disease (AD), the major dementing disorder of the elderly, is associated with cholinergic neuronal loss and decreased activity of choline acetyl-transferase (CAT). Previous biophysical studies had suggested an altered conformation of membrane proteins in AD erythrocyte ghosts. Since erythrocytes have a choline transport system and cholinergic neurons are implicated in AD, the present experiments were undertaken to determine if the efflux rate of [¹⁴C]choline was altered in AD erythrocytes. The mean efflux rate constant was highly significantly increased (P<0.01) by greater than 25% in 9 drug-free AD patients compared to 9 sex-matched, drug-free controls of similar age. These results are discussed in terms of potential molecular mechanisms to account for cholinergic neuronal loss in AD.     

中华白蛉的自育性研究

现场及实验室结果表明,我国黄土高原的大多数中华白蛉(Phlebotomus chinensis)应属自育性品系,它通常在羽化后、吸血前经交配能依靠腹节内脂肪体发育卵泡,一般在产卵后始行吸血。其生理性状是:胃内无血、腹节内有大量块状或条状脂肪体。羽化24小时后,附腺内有暗色颗粒,卵巢内有发育的卵泡。在25℃士1℃下它的生活史分快、慢两型。快型从卵至成虫仅需44—50夭,慢型需要以四龄幼虫滞育,其长短随滞育期而定,最长的滞育期达301天。观察了白蛉幼虫在饥饿状态下对自育性的影响。此外,还比较了吸血白蛉与自育性白蛉的妊卵数。吸血白蛉的妊卵数约较自育性的高出1/5。这种自育性品系的中华白蛉在自然居群约内占92%、主要栖于洞穴内为野栖种类。本文对自育性中华白蛉的生态及其防制策略作了分析和讨论。     

中国拟盘多毛孢属真菌的七个新组合

作者按照Steyaert(1949)和Sutton(1980)的分类观点,对1987—1988年从我国广西、江西、浙江等地采集的Pestalotiopsis属真菌进行了研究,确认了拟盘多毛孢属真菌的7个新组合种,即Pestalotiopsis carveri(Guba)comb nov.,Pestalotiopsis elasticae(Koord.)comb.nov.,Pestalotiopsis langloisii(Guba)comb.nov.,Pestalotiopsis oleandri(Guba)comb.nov.,Pestalotiopsis sinensis(shen)comb.nov.,Pestalotiopsis sydowiana(Bres.)comb.nov.和Pestalotiopsis zahlbruckneriana(Bres.)comb.nov.     

Oxygen transport during steady-state submaximal exercise in chronic hypoxia

Arterial O2 delivery during short-term submaximal exercise falls on arrival at high altitude but thereafter remains constant. As arterial O2 content increases with acclimatization, blood flow falls. We evaluated several factors that could influence O2 delivery during more prolonged submaximal exercise after acclimatization at 4,300 m. Seven men (23 +/- 2 yr) performed 45 min of steady-state submaximal exercise at sea level (barometric pressure 751 Torr), on acute ascent to 4,300 m (barometric pressure 463 Torr), and after 21 days of residence at altitude. The O2 uptake (VO2) was constant during exercise, 51 +/- 1% of maximal VO2 at sea level, and 65 +/- 2% VO2 at 4,300 m. After acclimatization, exercise cardiac output decreased 25 +/- 3% compared with arrival and leg blood flow decreased 18 +/- 3% (P less than 0.05), with no change in the percentage of cardiac output to the leg. Hemoglobin concentration and arterial O2 saturation increased, but total body and leg O2 delivery remained unchanged. After acclimatization, a reduction in plasma volume was offset by an increase in erythrocyte volume, and total blood volume did not change. Mean systemic arterial pressure, systemic vascular resistance, and leg vascular resistance were all greater after acclimatization (P less than 0.05). Mean plasma norepinephrine levels also increased during exercise in a parallel fashion with increased vascular resistance. Thus we conclude that both total body and leg O2 delivery decrease after arrival at 4,300 m and remain unchanged with acclimatization as a result of a parallel fall in both cardiac output and leg blood flow and an increase in arterial O2 content.(ABSTRACT TRUNCATED AT 250 WORDS)     

斜胸叶蝉属一新种（同翅目：叶蝉总科：耳叶蝉科）

本文记述耳叶蝉科斜胸叶蝉属一新种:黄缘斜胸叶蝉Epiclinata flavomarinata sp.nov.。标本采自我国西藏。文中描述了新种的外部形态及雄性外生殖器构造特征,并与近似种进行比较,附有主要特征图。模式标本保存于中国科学院动物研究所。     

Membrane-altering effects of velnacrine and N-methylacridinium: relevance to tacrine and Alzheimer's disease.

The interaction of pharmacological agents potentially useful in Alzheimer's disease, 9-amino-1,2,3,4-tetrahydroacridine (THA or tacrine) and its major metabolite velnacrine (or HP-029), along with related compounds with cytoskeletal proteins in human erythrocyte membrane was investigated using electron paramagnetic resonance spin labeling techniques. The results suggest that: (1) the position of the positive charge of tacrine may be important in the mechanism of its interaction with the membrane cytoskeleton; (2) like tacrine, velnacrine also strengthens cytoskeletal protein-protein interactions in erythrocyte membranes, but appears to be only about half as potent as tacrine. These results are discussed with relevance to therapeutic use of these agents in Alzheimer's disease.