饲料原料中转基因成分的PCR检测

采用PCR检测方法从饲料的主要原料豆粕、玉米蛋白粉中成功地检出启动子35S (35S-promoter,originated from cauliflower mosaic virus)、终止子NOS (nopaline synthase-terminator,derived from Agrobacterium tumefaciens)、耐除草剂基因EPSPS(5-enolpyruvylshikimate-3-phosphate synthase)和抗虫基因CryIA（b）(delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)等转基因成分,并通过扩增玉米自身蛋白基因Zein( a protein extracted from corn gluten)及大豆自身基因Lectin(chitin-binding protein)的引物和阴阳性对照、阴阳性质控,避免假阳性、假阴性结果。该方法已在口岸进口饲料原料转基因检测中得到初步应用。 Abstract:Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus),NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS(5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b)(delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal,respectively.Endogenous corn Zein(a protein extracted from corn gluten) gene,soybean Lectin(chitin-binding protein) gene and negative,positive control were applied for avoiding false results.The method established here has been succeessfully applied in detecting transgenic elements in imported feed raw material.