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以甜菜叶片为材料,用CTAB法提取基因组DNA.以分段PCR法扩增得到了完整的甜菜胞质型谷氨酰胺合成酶(GS1)基因组DNA.采用RT-PCR法扩增此GS1基因(GS1)的cDNA序列应用于对照.获得了长度为9 606bp的完整的GS1 DNA序列和长度为1 068 bp的GSI cDNA序列.分析GS1基因组DNA序列表明,它包含13个外显子,被12个内含子分隔开.其外显子区与已公布的GS1 mRNA序列的相似性达99.5%.RT-PCR法获得的cDNA序列与已知的GS1 mRNA序列相似性达99.6%.而2次实验中GS1基因组DNA外显子区与GS1 cDNA序列的相似性达99.9%.GenBank登录号为EU370974. 相似文献
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A line (named Cl) of cytoplasmic sterility of sugar beet whose cytoplasm derived from Betacicla Turkey was obtained by interspecific hybrid. Its cytoplasm and a spontaneous male sterile cytoplasm from wild beet Beta maritima (named M) were compared with that of Owen's sterile line (S-cms) and a common maintainer of them named N was used as control. RFLP and RAPD methods were mainly used in our experiments. The restriction fragment patterns of mtDNAs were found to be likely but for a few of specific low-lighted electrophoresis bands in Cl. The results of Southern hybridization of six heterogeneous mitochondrial genes as probes to digests of mtDNAs by six restriction enzymes showed to be analogous between S and M lines. But the Cl mtDNA was sorted out by hybridization of atpA probe. Difference of low-molecular-weight mitochondrial DNAs was found among the three sterile lines. Three RNA molecules weighing about 4.2kb stably existed in Cl mitochondria. Our results of RAPD also supported that the Cl cytoplas 相似文献
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一种新型甜菜胞质雄性不育系的分子生物学鉴定 总被引:2,自引:0,他引:2
对通过种间杂交获得的两种胞质雄性不育系甜菜(命名为Cl和M,其细胞质来源分别为Beta cicla Turkey和Beta maritima)和通常的Owen型胞质不育系甜菜(简称作S)以及这3种不育系通用的保持系(简称N)进行了线粒体基因组的RFLP和RAPD对比分析.结果表明Cl与S相比线粒体组织结构具有明显的差别.用6种异源线粒体基因作探针对6种限制性内切酶片段进行Southern杂交分析,发现Cl和S线粒体基因组之间存在明显多态性.但S和M之间却异乎寻常地表现一致.RAPD分析验证了Cl和 S之间的差异,此外,在Cl中发现 3种 4.2 kb大小的特异存在的RNA分子.根据结果认为Cl是一种不同于S的新型细胞质不育系.用RAPD方法也找到了S与M之间差异.酶切结果表明N与3个不育系的mtDNA非常相似.但在杂交和RAPD分析中均找到了N系的特异片段.这些片段是否与育性相关尚有待分析. 相似文献
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