Repeated pre-training sleep restriction in adolescent rats impaired spatial performance

Sleep and Biological Rhythms - Chronic sleep deprivation (SD) is an overwhelming problem in young students. Firstly, we investigated whether different levels of pre-training SD had effects on...     

Psychometric properties of Youth Self-Rating Insomnia Scale (YSIS) in Chinese adolescents

Sleep and Biological Rhythms - Insomnia is prevalent in adolescents. Although several insomnia scales/questionnaires are available to assess insomnia symptoms and severity for adults, no insomnia...     

CC类趋化因子亚家族与心肌梗死的研究进展

CC类趋化因子亚家族是趋化因子家族中成员最多、研究最广泛的一大类细胞因子,其主要功能参与炎症细胞激活、迁移、粘附等病理生理过程。大量研究表明,CC类趋化因子亚家族成员参与了心肌梗死后病理过程的各个阶段。其中研究最为深入的为单核细胞趋化蛋白-1（monocyte chemoattractant protein-1,MCP-1）及其受体CC趋化因子受体2（CC chemokine receptor 2,CCR2）,在心肌梗死后炎症期、增殖期及疤痕愈合期都发挥了重要作用从而影响梗死后心室重构。近年来,CC类趋化因子亚家族其他成员亦被逐渐揭示参与了心肌梗死的发展。本文结合以往大量文献将对CC类趋化因子亚家族在心肌梗死各个阶段中尤其是梗死后各期对于心室重构的影响进行综述,以期为今后的实验研究提供方向及疾病的预防和治疗提供药物靶点。     

外源保幼激素对中华通草蛉滞育解除及滞育后发育的影响

【目的】本研究旨在明确外源保幼激素(juvenile hormone, JH)在中华通草蛉Chrysoperla sinica滞育快速解除过程中的使用剂量及最佳使用时期。【方法】测定点滴不同剂量(0, 5, 15, 25和35 μg/成虫)外源JH后中华通草蛉滞育成虫的产卵前期、产卵历期、雌虫寿命及单雌产卵量,以及15 μg/成虫外源JH处理对不同日龄(0, 5, 10, 20, 30和40日龄)滞育成虫处理后这4个指标的变化。【结果】在不同点滴剂量处理中,15和25 μg/成虫外源JH处理的中华通草蛉滞育成虫的产卵前期分别为6.82和6.29 d,显著短于点滴丙酮的对照组(10.55 d),且15 μg/成虫外源JH处理组产卵历期、雌虫寿命及单雌产卵量均最大,均显著大于对照组。不同日龄的中华通草蛉滞育成虫点滴15 μg/成虫外源JH,0, 5, 10和20日龄处理的成虫产卵前期均显著短于未经外源JH处理的对照组。10, 20和30日龄处理的成虫产卵历期和雌虫寿命均与未经外源JH处理的对照组无显著差异,但10, 20和30日龄处理的成虫生殖力显著降低,而20日龄处理时对照组和外源JH处理组均有较高的生殖力。【结论】综合考虑产卵前期、产卵历期、产卵量和雌虫寿命4个指标,15 μg/成虫的外源JH剂量是中华通草蛉快速解除滞育的最佳剂量;最佳使用时期为滞育20 d的成虫,此时对中华通草蛉成虫滞育解除后的生殖能力影响最小。本研究解决了中华通草蛉滞育解除耗费时间长的瓶颈问题,为成虫滞育天敌的高效存储利用与滞育的快速解除提供了新思路。     

一种展示SARS冠状病毒受体结合区的重组枯草杆菌芽孢的制备及免疫原性分析

目的：利用枯草杆菌芽孢呈递技术制备表达SARS冠状病毒S蛋白受体结合区（RBD）的重组芽孢。方法：将枯草杆菌 CotB 基因构建到基因组整合质粒pDG1664中,再将 RBD 基因连接到 CotB 基因的下游,构建成重组质粒pDG1664-CotB-RBD,通过同源重组整合到PY-79枯草杆菌基因组中;利用红霉素抗性筛选重组菌并进行PCR和DNA测序鉴定,Western印迹鉴定重组菌芽孢表面RBD蛋白的表达情况;用表达RBD的重组芽孢以口服方式免疫小鼠,通过ELISA和流式细胞术检测重组芽孢的免疫原性。结果：制备出枯草杆菌基因组整合了RBD抗原基因的重组菌株RS1931,形成的重组芽孢表达相对分子质量约62×103的CotB-RBD融合蛋白;重组芽孢免疫的小鼠血清RBD抗原特异性IgG抗体滴度在末次免疫后2周可达1∶10880,重组芽孢初免后18周的小鼠脾细胞中IFN-γ＋CD4^＋、IL-4＋CD4^＋和IFN-γ＋CD8^＋T细胞比例上调,表明重组芽孢经口服免疫产生良好的体液免疫和细胞免疫应答。结论：针对SARS冠状病毒S蛋白RBD建立了枯草杆菌芽孢呈递技术方法,制备出在枯草杆菌芽孢表面稳定表达外源RBD蛋白的重组株,获得的重组芽孢具有良好的免疫原性,为开发芽孢呈递型SARS疫苗奠定了基础。     

β3 Integrin Promotes TGF-β1/H2O2/HOCl-Mediated Induction of Metastatic Phenotype of Hepatocellular Carcinoma Cells by Enhancing TGF-β1 Signaling

In addition to being an important mediator of migration and invasion of tumor cells, β3 integrin can also enhance TGF-β1 signaling. However, it is not known whether β3 might influence the induction of metastatic phenotype of tumor cells, especially non-metastatic tumor cells which express low level of β3. Here we report that H₂O₂ and HOCl, the reactive oxygen species produced by neutrophils, could cooperate with TGF-β1 to induce metastatic phenotype of non-metastatic hepatocellular carcinoma (HCC) cells. TGF-β1/H₂O₂/HOCl, but not TGF-β1 or H₂O₂/HOCl, induced β3 expression by triggering the enhanced activation of p38 MAPK. Intriguingly, β3 in turn promoted TGF-β1/H₂O₂/HOCl-mediated induction of metastatic phenotype of HCC cells by enhancing TGF-β1 signaling. β3 promoted TGF-β1/H₂O₂/HOCl-induced expression of itself via positive feed-back effect on p38 MAPK activation, and also promoted TGF-β1/H₂O₂/HOCl-induced expression of α3 and SNAI2 by enhancing the activation of ERK pathway, thus resulting in higher invasive capacity of HCC cells. By enhancing MAPK activation, β3 enabled TGF-β1 to augment the promoting effect of H₂O₂/HOCl on anoikis-resistance of HCC cells. TGF-β1/H₂O₂/HOCl-induced metastatic phenotype was sufficient for HCC cells to extravasate from circulation and form metastatic foci in an experimental metastasis model in nude mice. Inhibiting the function of β3 could suppress or abrogate the promoting effects of TGF-β1/H₂O₂/HOCl on invasive capacity, anoikis-resistance, and extravasation of HCC cells. These results suggest that β3 could function as a modulator to promote TGF-β1/H₂O₂/HOCl-mediated induction of metastatic phenotype of non-metastatic tumor cells, and that targeting β3 might be a potential approach in preventing the induction of metastatic phenotype of non-metastatic tumor cells.     

Identification of Surface Water Quality along the Coast of Sanya,South China Sea

Principal component analysis (PCA) and cluster analysis (CA) are utilized to identify the effects caused by human activities on water quality along the coast of Sanya, South China Sea. PCA and CA identify the seasonality of water quality (dry and wet seasons) and polluted status (polluted area). The seasonality of water quality is related to climate change and Southeast monsoons. Spatial pattern is mainly related to anthropogenic activities (especially land input of pollutions). PCA reveals the characteristics underlying the generation of coastal water quality. The temporal and spatial variation of the trophic status along the coast of Sanya is governed by hydrodynamics and human activities. The results provide a novel typological understanding of seasonal trophic status in a shallow, tropical, open marine bay.     

酿酒酵母Mbp1缺陷型菌株的构建及其乙醇发酵特性

【目的】研究酿酒酵母(Saccharomyces cerevisiae)工业菌株Mbp1基因的功能,探讨Mbp1基因对酿酒酵母乙醇发酵性能的影响。【方法】以酿酒酵母MF1015为出发菌株,用PCR方法构建Mbp1基因敲除组件Loxp-KanMX-Loxp,将敲除组件转化两种配型的酿酒酵母单倍体,通过单倍体复倍获得敲除Mbp1基因的二倍体突变菌株,研究突变菌株形态变化及乙醇发酵特性。【结果】敲除Mbp1基因后突变菌株生长曲线无显著变化,出芽率降低,细胞体积增大19.2%,对饥饿更敏感,较早出现假菌丝。甘蔗糖蜜在静置条件下发酵,突变菌株的乙醇产量明显低于野生型;在130 r/min的条件下发酵,突变菌株和野生型发酵液中的乙醇产量基本相同。【结论】Mbp1基因缺失使酿酒酵母的乙醇发酵能力下降并影响细胞的形态分化。     

共表达外源OCT4/SOX2能够激活人胚肾细胞中内源NANOG的转录

自2006年诱导多能干细胞（iPS）技术诞生以来,采用病毒等载体进行的诱导方法已取得了成功,但是其致瘤性的影响限制了病毒载体的推广与应用,而采用非病毒载体诱导iPS细胞成为研究的热点. 本研究通过两个启动子的独立启动,构建了带有绿色荧光标记的OCT4/SOX2共表达诱导载体(pOct4/Sox2-EGFP). 将该载体转染HEK 293FT 细胞后,阳性克隆明显表达绿色荧光,并通过RT-PCR,免疫荧光等方法证明其中的转录因子OCT4和SOX2能在转染细胞中高效表达,同时诱导受体细胞中内源NANOG的转录表达. 本研究说明OCT4/SOX2共表达载体能激活NANOG基因的内源表达,暗示着非病毒不整合载体pOct4/Sox2-EGFP本身或与其它转录因子和小分子结合可用于诱导成体细胞的重编程. 因此,本研究为下一步应用质粒载体诱导体细胞重编程为iPS细胞的研究奠定了工作基础.