植物蛋白SUMO化修饰检测方法

SUMO化是一种重要的蛋白质翻译后修饰,对植物正常生长发育不可或缺。到目前为止已筛选到上千个可能的SUMO底物,但由于SUMO化修饰水平普遍很低,其生物学功能研究相对较少。该文详细描述了检测蛋白SUMO化修饰的常用方法,包括体外和体内SUMO化实验,以及SUMO化修饰位点的检测方法,旨在为深入研究植物蛋白SUMO化修饰提供技术支持。     

中国省域生态文明建设进程区域差异化

中国生态文明理论体系和示范实践为我国各地全面推进生态文明建设奠定了基础,区域差异化研究希望通过探究不同区域生态文明建设特征、潜力以及与经济发展的联系,为我国各地区全面推进生态文明实践提供的支持。构建“社会、经济、政治、文化和生态”生态文明指数五维度评价指标体系,运用2005、2010、2015和2019年我国面板数据,刻画31个省份(统计数据尚未包含我国港澳台)生态文明建设的前期、初期、快速发展期和当前各时段时空变化,提出区域的发展速度和发展加速度概念描述各省份建设能力差异,基于各省份生态文明五维度预期目标和历史发展速度探究各省份的发展潜势差异,并初步探究各省份生态文明差异与经济发展的关系。研究结果表明：(1)总体上,各省份的生态文明指数反映出生态文明建设效果显著;时间序列变化上,各省份生态文明建设整体水平和过程存在较大差异性,尽管增长速度都呈现出先快后慢的特点,各省份生态文明水平差距仍然持续拉大;在空间上,明显呈现出东部地区好于西部地区、南部地区好于北部地区的特征,通过分析31个省份所属的六个地区的生态文明等级,发现华东地区和中南地区生态文明等级领先于其他地区,且在各阶段进步最大,而...     

肺心病患者血清甲状腺激素的变化

本文分析30例慢性肺心病心衰并呼衰患者(心衰并呼衰组)及30例慢性肺心病心衰无呼衰患者(心衰无呼哀组)和慢性肺心病死亡组的血清甲状腺激素水平。结果表明心衰并呼衰组T_3、T_4水平均值显著低于心衰无呼衰组和健康组,心衰无呼衰组T_3水平均值显著低于健康组,肺心病死亡组T_3、T_4水平均值显著低于存活组,并发现血清T_3、T_4水平与动脉血氧分压(PaO_2)呈正相关。作者认为T_3明显降低是重症肺心病的损伤性结果,预示病情严重,预后差。而T_4明显下降,可能是死亡的信号之一。     

黑龙江省东宁县山区蜱类的生态调查

我国东北地区有的蜱种已证实是传播森林脑炎、北亚蜱传斑疹伤寒的媒介。近年来,国内文献又报导了从东北牡丹江林区发现了莱姆病(Lyme Disease)病人,病人都有被婢咬史,并从全沟硬蜱(Ixodes persulcatus)的中     

Structure of intact human MCU supercomplex with the auxiliary MICU subunits

Dear Editor,Mitochondrial Ca2+homeostasis regulates energy production,cell division,and cell death.The basic properties of mitochondrial Ca2+uptake have been firmly established.The Ca2+influx is mediated by MCU,driven by membrane potential and using a uniporter mechanism(Vasington and Murphy,1962).Patch-clamp analysis of MCU currents demonstrated that MCU is a channel with exceptionally high Ca2+selectivity(Kirichok et al.,2004).     

Light-Regulated Hypocotyl Elongation Involves Proteasome-Dependent Degradation of the Microtubule Regulatory Protein WDL3 in Arabidopsis

Light significantly inhibits hypocotyl cell elongation, and dark-grown seedlings exhibit elongated, etiolated hypocotyls. Microtubule regulatory proteins function as positive or negative regulators that mediate hypocotyl cell elongation by altering microtubule organization. However, it remains unclear how plants coordinate these regulators to promote hypocotyl growth in darkness and inhibit growth in the light. Here, we demonstrate that WAVE-DAMPENED 2–LIKE3 (WDL3), a microtubule regulatory protein of the WVD2/WDL family from Arabidopsis thaliana, functions in hypocotyl cell elongation and is regulated by a ubiquitin-26S proteasome–dependent pathway in response to light. WDL3 RNA interference Arabidopsis seedlings grown in the light had much longer hypocotyls than controls. Moreover, WDL3 overexpression resulted in overall shortening of hypocotyl cells and stabilization of cortical microtubules in the light. Cortical microtubule reorganization occurred slowly in cells from WDL3 RNA interference transgenic lines but was accelerated in cells from WDL3-overexpressing seedlings subjected to light treatment. More importantly, WDL3 protein was abundant in the light but was degraded through the 26S proteasome pathway in the dark. Overexpression of WDL3 inhibited etiolated hypocotyl growth in regulatory particle non-ATPase subunit-1a mutant (rpn1a-4) plants but not in wild-type seedlings. Therefore, a ubiquitin-26S proteasome–dependent mechanism regulates the levels of WDL3 in response to light to modulate hypocotyl cell elongation.     

The influence of cathelicidin LL37 in human anti-neutrophils cytoplasmic antibody (ANCA)-associated vasculitis

Introduction

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is characterised by the autoinflammation and necrosis of blood vessel walls. The renal involvement is commonly characterised by a pauci-immune crescentic glomerulonephritis (PiCGN) with a very rapid decline in renal function. Cathelicidin LL37, an endogenous antimicrobial peptide, has recently been implicated in the pathogenesis of autoimmune diseases. To assess whether serum LL37 reflects renal crescentic formation, we measured the serum levels of LL37 in AAV patients with and without crescentic glomerulonephritis (crescentic GN) as compared to healthy controls (HCs). We also analysed the correlation of the serum levels of LL37 and interferon-α (IFN-α) with the clinical characteristics of the patients.

Methods

The study population consisted of 85 AAV patients and 51 HCs. In 40 ANCA-positive patients, a parallel analysis was performed, including the assessment of LL37 and IFN-α levels in the serum and renal biopsies. Of those studied, 15 AAV patients had biopsy-proven crescentic GN, and 25 AAV patients lacked crescent formation. The serum levels of cathelicidin LL37 and IFN-α were both measured by ELISA, and the clinical and serological parameters were assessed according to routine procedures. Immunofluorescence staining was performed on frozen sections of kidney needle biopsies from AAV patients with crescentic GN.

Results

The serum levels of LL37 and IFN-α were significantly increased in AAV patients with crescentic GN compared to AAV patients without crescentic formation and HCs, and patients with high LL37 and IFN-α levels were more likely to be in the crescentic GN group. The LL37 levels were positively correlated with the IFN-α levels, and both LL37 and IFN-α levels showed a positive correlation with serum creatinine and no correlation with complement C3. The renal tissue of crescentic GN patients showed expression of LL37 and IFN-α at the Bowman’s capsule and extracellular sites, suggesting the active release of LL37 and IFN-α.

Conclusions

Significantly higher levels of LL-37 and IFN-α were observed in AAV patients, particularly those with crescentic formation, and LL37 and IFN-α were expressed in the renal tissue of patients with crescentic GN. These data suggest that serum levels of LL37 and IFN-α may reflect both local renal inflammation and systemic inflammation.