排序方式: 共有85条查询结果,搜索用时 156 毫秒
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双梭孢虫草无性型的鉴定 总被引:9,自引:0,他引:9
从贵州省贵阳市黔灵山采集分别寄生于不同刺蛾茧上,宏观特征呈简单和分枝的双棱孢虫草,经对子囊孢子诱发微循环产孢研究确证,双包虫草的无性型为隔梭孢属一新种,双梭隔梭孢。 相似文献
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利用RACE技术获得了冠突散囊菌stf1基因的全长DNA序列。该序列全长3 029bp,开放阅读框长度为2 664bp,从114–2 908bp,在253bp处含有一个131bp的内含子,预测编码887个氨基酸。同源分析结果表明该基因与Snd1/p100转录因子同源。应用相对定量SYBR Green I荧光定量PCR技术对stf1基因在不同发育时期的表达量的差异进行了检测。结果表明,这个基因在子囊孢子时期的表达量最高,在分生孢子时期的表达量最低,相比子囊孢子时期下降了1倍。为深入研究冠突散囊菌的产孢调控机制奠定了一定的基础。 相似文献
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运用 PCR 法扩增湛江沿海海域7种龙虾的线粒体 CO玉和 Cyt b 基因并对其序列进行分析,以分析7种龙虾的分子系统关系.从 7种龙虾中扩增到的 CO玉基因片段长度均为650 bp,共存在224个核苷酸位点变异,变异率为35.22%,简约信息位点161个;扩增到的 Cyt b 基因片段长度均为536 bp,共存在148个核苷酸位点变异,变异率为29.48%,简约信息位点66个.所有的扩增序列中,没有发现碱基的缺失以及插入,序列中的转换大于颠换,且碱基替换多发生于密码子的第3位.以帝加洛真龙虾(Palinurus delagoae)、普通真龙虾(Palinurus elephas)、吉氏真龙虾(Palinurus gilchristi)为外群,对 CO玉和 Cyt b 两个基因序列采用最大简约法(maximum parsimony, MP)和贝叶斯推论法(bayesian inference, BI)构建龙虾类分子系统树.结果显示:日本龙虾和密毛龙虾亲缘关系比较近,而其它5种龙虾与真龙虾属的3种关系较近,与传统的分类存在一定分歧. 相似文献
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Li YANG Jiang CHEN Catherine C. Y. CHANG Xin-Ying YANG Zhen-Zhen WANG Ta-Yuan CHANG and Bo-Liang LI* State Key Laboratory of Molecular Biology Institute of Biochemistry Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai China Department of Biochemistry Dartmouth Medical School Hanover NH USA 《Acta biochimica et biophysica Sinica》2004,(4)
Acyl-coenzyme A:cholesterol acyltransferase (ACAT)is an integral membrane protein, which is mainly locatedin rough endoplasmic reticulum (ER), and is responsiblefor catalyzing the intracellular formation of cholesterylester from cholesterol and long-chain fatty acyl-coenzymeA [1,2]. Human ACAT1 cDNA K1 was firstly cloned andfunctionally expressed in 1993 [3]. Further studies withspecific anti-ACAT1 antibody (DM10) illustrated that onemajor 50 kD ACAT1 protein was expressed in various… 相似文献
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采集自贵州的两个稀有虫草 总被引:3,自引:0,他引:3
1994年6月采集自贵州梵净山和荔波茂兰保护区的虫草属真菌,中国新记录种-螳螂虫草(Cordycepsmantidaecolakob.etShim.)和属于蛇形虫草亚属头状组的一新种-贵州虫草(CordycepsguizhouensisLiu,LiangetLiusp.nov)贵州虫草寄生于刺蛾茧上,茧表面褐色,椭圆形,大小为4~6×5~9mm,在一个茧上可长1~2根子座,子座单生,不分枝,圆柱形 相似文献
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被孢霉γ-亚麻酸高产菌株选育 总被引:9,自引:1,他引:8
利用紫外线复合氯化锂诱变高山被孢霉SM96,筛选出一株高产γ-亚麻酸的菌株TM11,产量比出发菌株(25mg/L)提高了近3倍。对影响其多不饱和脂肪酸产生的因子Mg2+,VB6,营养盐溶液,磷源,碳源,氮源酵母膏进行了正交试验,选出TM11最佳的培养基配方:MgSO4·7H2O1.0g,VB6150mg/L,酵母膏6g/L,葡萄糖100g/L,营养盐溶液1mL/L,K2HPO42g/L。在上述最佳培养基中TM11的生物量达18.0213g/L,总脂达10.8128g/L,GLA量达668mg/L。 相似文献
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HENG CHUAN XIA FENG LI ZHEN LI Zu CHUAN ZHANG~* Key Laboratory of Proteomics Institute of Biochemistry Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China. 《Cell research》2003,(5)
A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of~29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC_(50) of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M_(21) with a IC_(50) of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent. 相似文献