极度濒危植物五针白皮松的保护遗传学研究

用随机扩增多态(RAPD)方法对极度濒危植物五针白皮松(Pinus squamata X.W.Li)的遗传多样性和居群遗传结构进行了研究。14个随机引物共获得93条RAPD谱带,其中6条为多态带,多态位点百分率仅为6．45％,遗传多样性极低。Shannon指数I和Nei指数h在种内也只有0．020和0．030,两个亚居群间(半阴坡亚居群与半阳坡亚居群)遗传分化程度不高,遗传分化系数Gst只有0．110,与大多数松科植物近似,居群每代迁移数为4．032。五针白皮松极低的遗传多样性可能是由于它在演化过程中遭受过严重的灾害,造成严重的瓶颈效应,丧失其大部分遗传变异。在随后的演化过程中由于遗传漂变、自交衰退等小种群现象,导致遗传多样性的进一步丧失。另外,阔叶树种对其生存的挤压和人类的干扰也是导致五针白皮松遗传多样性降低的因素之一。本文最后对该结果的保护生物学意义进行了评价。     

试述高等学校《植物学》单子叶植物部分教学内容的改革

高等学校《植物学》课程单子叶植物部分的主要教学内容包括：１）单子叶植物的主要特征;２）单子叶植物与双子叶植物特征的比较;３）单子叶植物的主要类群等。此外还庆集中讲授或强凋下列６个方面的主要内容;１）单子叶植物的习性及生境;２）单子叶植物的大类群和数目;３）单子叶植物的传统类群或“历史名称”;４０单子叶植物传粉受精以及演化趋势;５）单子叶植物的沼生型胚肥及全面胎座;６）单子叶植物生物学研究的新进展。     

广西植物新资料

报道了广西植物分布新记录属 1个 ,新记录种 19个。     

滇池水-植物系统金属元素的分布特征和相关性研究

对滇池优势水生植物凤眼莲、旱生莲子草、红线草、聚草和茭草及其对应水环境中的Ｋ、Ｎａ、Ｃａ、Ｍｇ、Ｆｅ、Ｍｎ、Ｚｎ、Ｃｕ、Ｐｂ、Ｃｄ进行了化学分析,初步揭示了滇池水－植物系统金属元素的分布特征和关性。结果表明,滇池水域属Ｋ、Ｃａ、Ｍｇ、Ｆｅ高背景生物地球化学区,植物体内金属元素的含量,既与植物种属特性有关,又明显地受环境因素的影响,植物对营养元素的吸收不一定与环境中相应元素的浓度呈简单的线性关系,而     

植物细胞程序死亡的机理及其与发育的关系

细胞程序死亡（ＰＣＤ）是在植物体发育过程中普遍存在的,在发育的特定阶段发生的自然的细胞死亡过程,这一死亡过程是由某些特定基因编码的“死亡程序”控制的。ＰＣＤ的细胞分化的最后阶段。细胞分化的临界期就牌死亡程序执行中的某个阶段。ＰＣＤ包含启动期和清除期三个阶段,其间ＣＡＳＰＡＳＥ家族起着重要作用。ＰＣＤ在细胞和组织的平衡、特化,以及组织分化、器官建成和对病原体的反应等植物发育过程中起着重要作用。ＰＣＤ     

Human μ—opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi／0 proteins

Human mu-opioid receptor (HmuOR) with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect cells. The maximal binding capacity for the [3H] diprenorphine and [3H]ohmefentanyl (Ohm) were 9.1 +/- 0.7 and 6.52 +/- 0.23 nmol/g protein, respectively. The [3H] diprenorphine or [3H] Ohm binding to the receptor expressed in Sf9 cells was strongly inhibited by mu-selective agonists [D-Ala2, N-methyl-Phe4, glyol5]enkephalin (DAGO), Ohm, and morphine, but neither by delta nor by kappa selective agonist. Na+ (100 mM) and GTP (50 microM) could reduce HmuOR agonists etorphine and Ohm affinity binding to the overexpressed HmuOR. mu-selective agonists DAGO and Ohm effectively stimulated [35S]GTP-gammaS binding (EC50 = 2.7 nM and 6.9 nM) and inhibited forskolin- stimulated cAMP accumulation (IC50 = 0.9 nM and 0.3 nM). The agonist-dependent effects could be blocked by opioid antagonist naloxone or by pretreatment of cells with pertussis toxin (PTX). These results demonstrated that HmuOR overexpressed in Sf9 insect cells functionally coupled to endogenous G(i/o) proteins.     

鼠白细胞介素12（mIL—12）在昆虫细胞中的表达

Since human IL-12 is species-specific in its functions and elicits little biological responses from mouse lymphocytes, it is necessary to express recombinant murine IL-12 for the usage in studying the effects of this cytokine in various rodent models. Thereby, we can investigate the role of IL-12 in immune response in vivo and evaluate its potential clinical utility. Thus, we firstly constructed two expression vectors, pVL1393-mp40 and pVL1393-mp35. They were used to co-transfect the insect cells(Sf9) separately with linearized polyhedrosis virus genomic DNA. Two kinds of recombinant viruses AcNPV-mp40 and AcNPV-mp35 were visually screened out, and mp40 and mp35 were co-expressed in the insect cells co-infected by AcNPV-mp40 and AcNPV-mp35. The results of real-time Biomolecular Interaction Analysis (BIA) and Northern blot demonstrated that the recombinant mIL-12 was expressed successfully in the insect cells. The molecular weights of recombinant mp40 and mp35 were 40 KDa and 22 KDa on SDS-PAGE under reducing conditions, respectively. The apparent molecular weight of recombinant mIL-12 is 80 KDa under non-reducing conditions of Western blot. Biological activity of the recombinant product was detected in conditional medium using antibody-capture bioassay. The expression level of recombinant mIL-12 was about 10-15 micrograms/10(6) cells, as compared with the calibration curve of mIL-12.     

昆虫幼虫储存蛋白

本文对昆虫幼虫血淋巴内一种特殊性质的蛋白－幼虫储存蛋白的命名、生化特性、合成部位、生理作用和体内脂肪体合成、贮存和利用的调节机制等方法的研究进展进行了综述。     

植物远缘杂交的障碍及其克服

植物的远缘杂交是指种以上分类单位的生物类型之间的杂交,包括同属植物的种间杂交和不同属植物的属间杂交。通过植物的远缘杂交可将其它种的有益性状（如抗病、抗虫、抗逆性强、优质）的基因资源导入栽培种,在作物、蔬菜和花卉育种中广泛应用。因而它是育种工作的一个重...