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71.
Dagui Zhou Dandan Xie Fangcheng He Baoan Song Deyu Hu 《Bioorganic & medicinal chemistry letters》2018,28(11):2091-2097
A new concise and facile method was explored to synthesize a series of novel chalcone derivatives containing a purine and benzenesulfonamide moiety and their antiviral properties were evaluated against TMV and CMV. Biological assays indicated that several of the derivatives exhibited significant anti-TMV and anti-CMV activities in vivo. In particular, compound d2 displayed excellent inactivating activity against TMV, with the EC50 value of 51.65?μg/mL, which was better than that of ribavirin (150.45?μg/mL). Molecular docking showed that there are four hydrogen bonds between compound d2 and TMV coat protein (TMV-CP). Compound d2 demonstrated strong binding capacity to TMV-CP with Ka?=?1.58?×?105?L/mol and Kd?=?12.16?μM. These findings indicated that chalcone derivatives are worthy of further research and development as templates for new antiviral agents. 相似文献
72.
LITTLELEAF (LL) encodes a WD40 repeat domain‐containing protein associated with organ size variation in cucumber 下载免费PDF全文
73.
The enantioselective degradation of benalaxyl has been investigated to elucidate its behavior in several agricultural soils and plants (cucumber). Racemic benalaxyl was fortified into five types of agricultural soils and sprayed leaves of cucumber plants, respectively. The degradation kinetics and the enantiomer fraction (EF) were determined by normal-phase high-performance liquid chromatography (HPLC) with diode array detection (DAD) on the chiral column filled cellulose-tri-(3,5-dimethylphenylcarbamate)-based chiral stationary phase (CDMPC-CSP). The process of the degradation of benalaxyl enantiomers followed pseudo-first-order kinetics in cucumber plant. However, the dissipation phases of benalaxyl enantiomers in soils were biphasic ("slow-fast-slow" process). It has been shown that the degradation of benalaxyl was stereoselective. The results indicated that the (+)-S-benalaxyl showed a faster degradation in plants, while the (-)-R-benalaxyl showed a faster degradation in Soils 3, 4, and 5. No stereoselective degradation was observed in other soils. 相似文献
74.
75.
FENG ZHU HE TANG XIN LV JIAN CHENG HUANG‐FAN XIE HONG‐HUI LIN 《Plant, cell & environment》2012,35(11):1983-1997
The effects of potassium cyanide (KCN) pretreatment on the response of cucumber (Cucumis sativus L.) plants to salt, polyethylene glycol (PEG) and cold stress were investigated in the present study. Here, we found that KCN pretreatment improved cucumber seedlings tolerance to stress conditions with maximum efficiency at a concentration of 20 µM. The results showed that pretreatment with 20 µM KCN alleviated stress‐induced oxidative damage in plant cells and clearly induced the activity of alternative oxidase (AOX) and the ethylene production. Furthermore, the structures of thylakoids and mitochondria in the KCN‐pretreated seedlings were less damaged by the stress conditions, which maintained higher total chlorophyll content, photosynthetic rate and photosystem II (PSII) proteins levels than the control. Importantly, the addition of the AOX inhibitor salicylhydroxamic acid (1 mm ; SHAM) decreased plant resistance to environmental stress and even compromised the cyanide (CN)‐enhanced stress tolerance. Therefore, our findings provide a novel role of CN in plant against environmental stress and indicate that the CN‐enhanced AOX might contribute to the reactive oxygen species (ROS) scavenging and the protection of photosystem by maintaining energy charge homoeostasis from chloroplast to mitochondria. 相似文献
76.
采用营养液培养方法,以耐盐性较弱的‘津春2号’黄瓜品种为试材,研究了等渗Ca(NO3)2和NaCl胁迫对黄瓜幼苗生长、根系电解质渗透率、根系活力、Na+和K+含量及渗透调节物质含量的影响。结果显示:(1)在84mmol.L-1 NaCl和56mmol.L-1 Ca(NO3)2等渗胁迫下,黄瓜幼苗鲜重和干重均显著下降,且NaCl处理下降的幅度大于等渗Ca(NO3)2处理。(2)NaCl主要通过对黄瓜根系的伤害来抑制植株生长,表现为根系活力下降、根系质膜透性增大、Na+大量积累、K+含量显著下降、Na+/K+明显上升,最终导致根冠比下降;而Ca(NO3)2处理对根系质膜透性、K+含量、Na+/K+的影响均小于NaCl胁迫,且根系活力和根冠比上升,但Ca(NO3)2胁迫后叶片含水量和渗透调节能力均小于NaCl胁迫。(3)NaCl胁迫条件下,黄瓜幼苗内渗透调节物质以可溶性糖为主,而Ca(NO3)2胁迫以可溶性蛋白为主。研究表明,NaCl胁迫对黄瓜幼苗的伤害大于等渗Ca(NO3)2,NaCl主要通过破坏根系质膜结构影响植株生长,而Ca(NO3)2主要通过引起地上部生理干旱来影响植株生长。 相似文献
77.
The ubiquitin proteasome system (UPS) is the main proteolytic system of cells. Recent evidence suggests that the UPS plays a regulatory role in regeneration processes. Here, we explore the possibility that the UPS is involved during intestinal regeneration of the sea cucumber Holothuria glaberrima. These organisms can regenerate most of their digestive tract following a process of evisceration. Initially, we identified components of H. glaberrima UPS, including sequences for Rpn10, β3, and ubiquitin-RPL40. Predicted proteins from the mRNA sequences showed high degree of conservation that ranged from 60% (Rpn10) to 98% (Ub-RPL40). Microarrays and RT-PCR experiments showed that these genes were upregulated during intestinal regeneration. In addition, we demonstrated expression of alpha 20S proteasome subunits and ubiquitinated proteins during intestinal regeneration and detected them in the epithelium and connective tissue of the regenerating intestine. Finally, the intestinal regeneration was altered in animals treated with MG132, a proteasome inhibitor. These findings support our contention that proteasomes are playing an important role during intestinal regeneration. 相似文献
78.
Na2CO3胁迫对黄瓜幼苗生长及生理指标的影响 总被引:3,自引:1,他引:2
以‘津优一号’和‘春四’黄瓜为实验材料,采用砂培法研究了Na2CO3胁迫对黄瓜幼苗生长及生理指标的影响。结果显示,随着Na2CO3浓度的增大,两个黄瓜品种的幼苗根冠比逐渐下降,叶绿素含量先上升后下降,可溶性糖含量、脯氨酸含量、SOD活性逐渐上升;‘津优一号’的相对电导率和丙二醛含量随Na2CO3浓度的增大逐渐增加,而‘春四’的相对电导率和丙二醛含量在20 mmol.L-1时最低,此后逐渐上升。主成分分析结果显示,丙二醛含量、SOD活性、可溶性糖含量、脯氨酸含量、叶绿素b,这5项指标在评价黄瓜幼苗的Na2CO3耐性方面是比较重要的。结合这5项指标综合分析,发现‘津优一号’较‘春四’有较强的耐碱性。 相似文献
79.
黄瓜叶片衰老过程中抗坏血酸含量与生理指标关系的研究 总被引:3,自引:0,他引:3
研究了黄瓜叶片衰老过程中抗坏血酸(ASA)含量及相关生理指标的变化,进一步分析了ASA与各相关生理指标之间的关系。结果显示,随着叶片逐渐衰老,丙二醛(MDA)含量、相对电导率(REC)及过氧化物酶(POD)活性显著升高;光合色素、可溶性蛋白质含量、超氧化物歧化酶(SOD)与过氧化氢酶(CAT)活性及ASA含量显著降低;ASA与MDA含量、REC、POD活性之间呈显著负相关关系,ASA与CAT及SOD活性之间呈显著正相关关系。研究表明,ASA对减缓叶片衰老有重要作用。 相似文献
80.