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91.
92.
The subcellular localization of lipoxygenase (LOX) from cucumber fruit has been studied. Two methods have been employed to obtain organelles; (1) maceration of the tissue, followed by separation on a linear sucrose gradient and (2) release from protoplasts by osmotic shock, followed by a discontinuous Ficoll gradient. It was possible to obtain high LOX activity in the intact protoplasts from both peel and flesh tissue. However, fewer intact vacuoles were obtained following osmotic rupture than from macerated tissue. Both methods produced more particulate LOX activity from the peel than from flesh tissue, and both showed that this activity was associated with the vacuoles. The cucumber LOX enzyme was similar to the potato and tomato enzymes, both in pH characteristics and substrate specificity. 相似文献
93.
Abscisic acid (ABA) is known to function in plant stress responses and seed dormancy, and much is known about its detailed mechanisms of signal transduction. Recent studies suggest that this hormone may also play important roles in sugar signaling and assimilate distribution during fruit development. However, little is known about the role of ABA in actively growing or differentiating fruits and other plant organs or tissues. To explore whether ABA functions during the early development of reproductive organs, we carried out ABA immunolocalization using monoclonal antibodies. The specific ABA accumulation pattern was verified by gas chromatography-mass spectrometry (GC-MS). ABA was not only detected in primordial cells of flower organs, but was also detected in nursing cells (e.g. tapetum and integuments), which function in supplying nutrition for germ cell development. These findings suggest that, in addition to its well-known function as a 'negative hormone', ABA may play some 'positive' roles during plant development, including possible involvement in the regulation of assimilate distribution. 相似文献
94.
24-表油菜素内酯对低氧胁迫下黄瓜根系抗氧化系统及无氧呼吸酶活性的影响 总被引:14,自引:0,他引:14
用营养液水培,研究了根际低氧胁迫下24-表油菜素内酯(EBR)对2个抗低氧能力不同的黄瓜品种根系中抗氧化系统及无氧呼吸酶活性的影响。结果表明,在低氧胁迫下,EBR处理显著提高了低氧胁迫下2品种黄瓜幼苗根系SOD、POD及ADH活性,降低了O2-·、H2O2和MDA含量、LDH活性及‘中农八号’根系PDC活性,而对‘绿霸春四号’根系PDC及2个品种CAT活性无明显影响,表明外源EBR处理通过促进低氧胁迫下根系中抗氧化酶和ADH活性的提高,降低LDH活性及ROS含量,增强植株抗低氧胁迫的能力。 相似文献
95.
Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression,
from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final
stage of senescing cucumber cotyledons (<95% yellow) for studying the genes responsible for lipid mobilization during germination
and senescence. This library had produced numerous senescence-associated clones in a previous study. Here, a total of 365
cDNA clones and their expression levels were examined via semi-quantitative RT-PCR. Up-regulation of expression was detected
for several known and unknown genes. These results were used to investigate the possible functions for senescence-related
genes during cotyledon development 相似文献
96.
Induction of larval metamorphosis in the sea cucumber Stichopus japonicus by periphitic diatoms 总被引:3,自引:0,他引:3
The mass production of juvenile seeds of the sea cucumber,Stichopus japonicus has recently developed by the SeaFarming Center of Saga Prefecture. Methods for the culture ofperiphicic diatoms have been improved. There are three importantsteps in propagating the diatoms. The first step is theenrichment, with the addition of the nutrient salts, undercontrolled light intensity. The second step is washing withhigh pressure seawater and reversal of the plates. The laststep is elimination of copepods, which feed on diatoms, usinga pesticide. Small periphitic diatoms such as Navicula,Amphora, Achnanthes, and Nitzschia are easily culturedat a density of more than one million cells cm–2, andthese diatoms are able to induce larval metamorphosis andserve as a food source for juvenile sea cucumbers. 相似文献
97.
Yuichi Takeuchi Reiko Fukumoto Hirokazu Kasahara Takeshi Sakaki Mitsutoshi Kitao 《Plant cell reports》1995,14(9):566-570
Cotyledons excised from dark-grown seedlings of cucumber (Cucumis sativus L.) were cultured in vitro under UV radiation at different wavelengths, obtained by passage of light through cut-off filters with different transmittance properties. Growth and the synthesis of chlorophyll (Chl) in cotyledons were inhibited and malondialdehyde was accumulated upon irradiation at wavelengths below 320 nm. Exogenous application of scavengers of free radicals reversed the growth inhibition induced by UV-B. Measurement of the fluorescence of Chl a suggested that electron transfer in photosystems was affected by UV-B irradiation. On the basis of these results, the involvement is postulated of active species of oxygen in damages to thylakoid membranes and the growth inhibition that are induced by UV-B irradiation.Abbreviations Chl
chlorophyll
- Fm
maximal fluorescence (dark)
- Fm
maximal fluorescence (light)
- Fv
variable fluorescence (dark)
- Fv
variable fluorescence (light)
- MDA
malondialdehyde
- O2
Superoxide radical
- PS
photosystem
- qN
non-photochemical quenching of fluorescence
- qP
photochemical quenching of fluorescence
- UV-BBE
biologically effective UV-B radiation
- WL(T = 0.5)
wavelength at which 50% transmittance occurs 相似文献
98.
Y. Yie Z. X. Wu S. Y. Wang S. Z. Zhao T. Q. Zhang G. Y. Yao P. Tien 《Transgenic research》1995,4(4):256-263
A procedure for the fast production of homozygotic transgenic plants was developed. Leaf discs of haploid tobacco plants from anther cultures were transformed with a chimaeric vector containing coat protein (CP) and satellite RNA (Sat-RNA) genes from cucumber mosaic virus (CMV). One-hundred-and-twelve Kanamycin-resistant transformed haploid plants were subjected to selection based on the expression of both CP and Sat-RNA. Eighty-nine transgenic plants expressing both genes were selected and tested for their resistance to CMV by inoculation with high concentration of CMV (200 g ml–1). Only five plants showed no symptoms of viral infection 30 days after inoculation. These plants were then diploidized by colchicine treatment. Three homozygous diploid lines with high levels of resistance to CMV were obtained after only one generation. The three transgenic lines were further tested under field conditions. The results showed that the progenies of these transgenic lines were homozygous and were highly resistant to CMV under natural field infection and manual inoculation conditions. 相似文献
99.
100.
M. J. van Steenis K. A. M. H. El-Khawass 《Entomologia Experimentalis et Applicata》1995,76(2):121-131
Life table data forAphis gossypii Glover (Homoptera: Aphididae), an important pest in glasshouse cucumber crops, were studied at 20, 25 and 30°C on two cucumber
cultivars (Cucumis sativus L.) in controlled climate cabinets. The development time on the cucumber cv. ‘Sporu’ ranged from 4.8 days at 20°C to 3.2
days at 30°C. Immature mortality was approximately 20% and did not differ between temperatures. Most mortality occurred during
the first instar. Reproduction periods did not differ among temperatures, but at 25 and 30°C more nymphs were produced (65.9
and 69.8 nymphs/♀, respectively) than at 20°C (59,9 nymphs/♀) because of a higher daily reproduction. Intrinsic rate of increase
was greatest at 25°C (r
m
=0.556 day−1). At 20 and 30°C the intrinsic rate of increase was 0.426 and 0.510, respectively. On cv. ‘Aramon’, the development time
ofA. gossypii was approximately 20% longer at all temperatures. Immature mortality did not differ between the two cultivars. The intrinsic
rate of increase on cv. ‘Aramon’ was 15% smaller than on cv. ‘Sporu’. The use of cucumber cultivars partially resistant to
aphids is discussed in relation to biological control of cotton aphid in glasshouses. Development time and immature mortality
on leaves of the middle and upper leaf layer of glasshouse grown cucumber plants (cv. ‘Aramon’) were comparable to development
in the controlled climate cabinets. On the lower leaves immature mortality was much higher (approximately 82%) than on leaves
of the middle (24.0%) and upper leaf layer (24.5%). Reproduction was less on the lower leaf layer (45.9, 70.5 and 70.1 nymphs/♀
on leaves of the lower, middle and upper leaf layer, respectively).
Aphids, successfully parasitized byAphidius colemani Viereck (Hymenoptera: Braconidae) only reproduced when they were parasitized after the third instar. Fecundity was 0.1 to
0.9 and 10.5 to 13.3 nymphs/♀ for aphids parasitized in the fourth instar or as adults, respectively. Reproduction of aphids
that were stung but survived the attack was lower than for aphids not stung. Average longevity of these aphids was equal to
the longevity of aphids not stung byA. colemani. 相似文献