全文获取类型
收费全文 | 218篇 |
免费 | 1篇 |
国内免费 | 1篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2015年 | 3篇 |
2014年 | 7篇 |
2013年 | 6篇 |
2012年 | 2篇 |
2011年 | 3篇 |
2010年 | 3篇 |
2009年 | 22篇 |
2008年 | 31篇 |
2007年 | 36篇 |
2006年 | 12篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2003年 | 2篇 |
2002年 | 4篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 9篇 |
1998年 | 15篇 |
1997年 | 13篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1986年 | 2篇 |
1984年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有220条查询结果,搜索用时 15 毫秒
21.
Enhancement of somatic embryogenesis and plant regeneration in Japanese larch (<Emphasis Type="Italic">Larix leptolepis</Emphasis>) 总被引:1,自引:0,他引:1
Different nitrogen sources, abscisic acid (ABA), gellan gum at various concentrations, and osmotica were evaluated for their effects on maturation of somatic embryo (SE) in Japanese larch (Larix leptolepis). Different concentrations of l-glutamine or casein hydrolysate (CH) in the medium were also compared. The highest number of matured embryos was obtained with ½ Litvay (LM) medium supplemented with 1.71 mM l-glutamine and 250 mg l?1 CH. In terms of osmoticum effect, the highest number of cotyledonary SEs was produced in medium containing 0.2 M maltose. As for the effects of ABA and gellan gum concentration, the highest number of cotyledonary SEs was achieved on a medium containing 60 μM ABA and 0.8% gellan gum. In addition, the best plantlet conversion frequency (35.5%) was obtained with SEs derived from the treatment with 60 μM ABA and 0.8% gellan gum. 相似文献
22.
In vitro screening of potato against water-stress mediated through sorbitol and polyethylene glycol 总被引:1,自引:0,他引:1
With the objective to develop a practical and effective method of screening potato for drought tolerance, shoot and root growth
in microtuber-derived plantlets was studied in vitro in three genotypes with known root mass production under field conditions.
Different levels of water-stress were induced using five concentrations of either sorbitol or polyethylene glycol (PEG) in
MS medium. Water potential of various media ranged from −0.80 MPa to −2.05 MPa. Water-stress in culture adversely affected
plantlet growth, and genotypes differed for their responses. Genotype IWA-1 was less affected than IWA-3 and IWA-5. At the
same level of water potential, sorbitol had lower adverse effect than PEG; the latter being sticky. Genotype × sorbitol and
genotype × PEG interactions were significant. At 0.2 M sorbitol and 0.003 M PEG, IWA-1 had significantly more roots with higher
total root length, root volume, as well as root-dry weight than those of IWA-3 and IWA-5, whereas the latter two genotypes
were at par for all these characters. This pattern was similar to the reported pattern of these genotypes for root-dry weight
under field conditions. It is concluded that in vitro screening of potato under specific and limited water-stress conditions
may provide a system for effectively differentiating the genotypes for their expected root mass production under field conditions. 相似文献
23.
David?NoshadEmail author Saber?Miresmaili Andrew?Riseman Abul?Ekramoddoullah 《Plant Cell, Tissue and Organ Culture》2009,96(2):201-209
Cultures of seven Daphne species: Daphne caucasica, D. cneorum, D. giraldii, D. retusa, D. jasminea, D. laureola and D. tangutica were established in vitro on MS/WPM based media. Five of the species responded best on MS-based media (D. tangutica, D. laureola, D. caucasica, D. retusa and D. giraldii), while the remaining two species performed best on WPM-based media (D. cneorum, and D. jasminea). Shoot proliferation was achieved from both apical and nodal explants. Shoots were sub-cultured from stock cultures, cut
into nodal explants 3–5 cm long and place vertically on basal media supplemented with different concentrations and combinations
of cytokinins and auxins. Individual species displayed different responses to the various cytokinins and auxins. Among species,
D. jasminea produced the greatest proliferation rate with an average of 7.84 + 0.6 shoots per explant on WPM supplemented with 2.32 μM
BA + 0.0045 μM TDZ + 0.054 μM NAA, while the best multiplication rate for the same species grown on the same media supplemented
with a single cytokinin (BA) and no auxin was 2.60 + 1.3 shoots per explant. Following multiplication, new shoots transferred
to the elongation trails and then 50–100 mm Shoots used for rooting experiments. Increased rooting efficiencies were observed
on in vitro-generated shoots with the two-layer medium or dipping methods over when PGRs were uniformly incorporated into
the medium. Maximum rooting frequencies (average) ranged from 59% in D. tangutica to 85% in D. jasminea. Following in vitro rooting, rooted shoots immersed in 0.01% solution of humates and planted into a standard horticultural
substrate composed and watered weekly with a solution containing half-strength MS salts. 相似文献
24.
Micropropagation was assessed as an ex situ conservation strategy for the endangered Australian plant Pimelea spicata (Thymelaeaceae). Although regeneration of this species was achieved, several physiological problems were observed and examined. Explants of P. spicata had a higher multiplication rate on MS medium, than on ½ MS, but there was a significantly higher percentage of necrotic shoot tips on the higher salt medium. Increasing calcium concentration and gas exchange exacerbated shoot-tip necrosis. A number of hyperhydrated shoots were produced in all treatments, the cause of which could not be determined, although less hyperhydicity was observed in the ½ MS treatment. Shoots, rooted in vitro on ½ MS in the absence of plant growth regulators, were successfully acclimatised to greenhouse conditions, while direct rooting of microshoots using IBA gel treatment proved unsuccessful. This is the first report of tissue culture propagation of this endangered species. 相似文献
25.
Mack?Moyo Jeffrey?F.?Finnie Johannes?Van?StadenEmail author 《Plant Cell, Tissue and Organ Culture》2009,98(3):273-280
Sclerocarya birrea (marula) is an indigenous South African tree with highly valued medicinal and nutritional properties. Induction of nodular
meristemoids from leaf explants was achieved on Murashige and Skoog (MS) and woody plant medium (WPM) supplemented with 6-benzyladenine
(BA) in combination with naphthalene acetic acid (NAA), indole-3-butryric acid (IBA) and indole-3-acetic acid (IAA). Induction
of nodular meristemoids from 86% of the leaf cultures was achieved on MS medium with 4.0 μM BA and 1.0 μM NAA. High levels
(78–100%) of induction were also achieved on WPM with different concentrations of BA (1.0–4.0 μM) and IBA (1.0–4.0 μM). The
highest conversion of meristemoids into shoots was only 22% for 4.0 μM BA and 1.0 μM NAA on MS initiation medium. This was
improved to 62% when nodular clusters were cultured in a MS liquid medium. Histological studies revealed the globular stage
of the nodular meristemoids. This protocol has potential for application in mass micropropagation and plant breeding of S. birrea. 相似文献
26.
Shoot cultures of Tamarix tetrandra on Linsmaier–Skoog (LS) agar medium with 30 g l−1 sucrose, 2.13 mg l−1 indoleacetic acid and 2.25 mg l−1 benzyl adenine produced ellagitannins found in intact plants of the Tamaricaceae. This was demonstrated by the isolation of 14 monomeric–tetrameric ellagitannins from the aq. Me2CO extract of the cultured tissues. This is the first report on the production of ellagitannin tetramers by plant tissue culture. The effects of light and certain medium constituents on tissue growth and ellagitannin production were examined. The contents of representative tannins of different types [i.e., tellimagrandin II (monomer), hirtellin A (linear GOG-type dimer), hirtellin B (hellinoyl-type dimer), hirtellin C (macrocyclic-type dimer), and hirtellin T1 (linear GOG-type trimer)] in the resultant tissues in response to these factors were estimated by HPLC, and the optimal condition for production of these tannins were established. Shoots cultured on LS hormone-free medium promoted root development, and regenerated plants could adapt to ordinary soil and climate. Acclimatized and intact T. tetrandra plants that were collected in November and May, respectively, demonstrated seasonal differences in individual ellagitannin contents. HPLC comparison of individual ellagitannin contents in different plant materials (i.e., leaves, stems, and roots) of intact T. tetrandra plants is also reported. The results are discussed with respect to cellular deposition and biosynthetic relationship of tannins. 相似文献
27.
A highly efficient three-step protocol for in vitro propagation of Ensete ventricosum (enset) was developed that consisted of initiation, bud proliferation, and shoot elongation and rooting stages. At the initiation stage, it was crucial to use shoot tips (5–8 mm) with subtending corm tissues as explants to obtain growth. The addition of 0.5–1% (w/v) activated charcoal to the medium was essential to prevent phenol exudation which otherwise leads to the loss of cultures. During the bud proliferation stage, modified MS macronutrients and micronutrients together with a combination of cytokinins (1.6 M naphthaleneacetic acid, 4.4 M 6-benzylaminopurine, 23.2 M kinetin, 22.6 M N6 2-isopentyladenine) was used. This novel composition of macronutrients was based on the analysis of leaf nutrient content of glasshouse-grown enset sprouts. Multiple bud formation on the enlarged corm tissue was induced only when the meristem region was wounded before transfer to the bud proliferation medium. Up to 75 healthy shoots per explant were produced, whereas unwounded explants produced, only one to two shoots per explant. A third stage with a low concentration of cytokinin enabled shoot elongation as well as root development. The plantlets were acclimatized with 100% success and they showed no apparent phenotypical deviation.Abbreviations BAP 6-Benzylaminopurine - IBA Indole-3-butyric acid - DW Dry weight - EV Ensete ventricosum medium - 2-iP N6 2-Isopentyl adenine - NAA -Naphthaleneacetic acid 相似文献
28.
C. Srinivasa Rao P. Eganathan A. Anand P. Balakrishna T. P. Reddy 《Plant cell reports》1998,17(11):861-865
An in vitro propagation protocol has been developed for Excoecaria agallocha L. (Euphorbiaceae), a mangrove species. Nodal segments were used for axillary shoot proliferation. One shoot from each node
of binodal explants was observed 3 weeks after inoculation. The best axillary sprouting was seen on a newly formulated medium
containing BA, Zeatin and IBA in concentrations of 13.3 μM, 4.65 μM and 1.23 μM, respectively. The new medium, first used in this study, has a specific composition of major nutrients, MS micronutrients
and iron compounds. Nodal segments from rooted cuttings and seedlings responded better than those of mature tree explants.
Multiple shoot induction was complemented with efficient shoot elongation, and repeated subculture of binodal segments from
axillary shoots resulted in 10–12 shoots per explant in 3 months. Rooting was achieved by growing shoots in the new medium
with 0.23 μM IBA. Regenerated plants were successfully acclimatized to the natural environment, and about 85% of plantlets survived under
ex vitro conditions. This is the first report of micropropagation in the genus Excoecaria and also in mangrove tree species.
Received: 11 August 1997 / Revision received: 10 February 1998 / Accepted: 2 March 1998 相似文献
29.
A protocol is presented for micropropagation of an economically important timber-yielding forest tree, Dalbergia sissoo Roxb. (Sissoo). Multiple shoots were induced from cotyledonary nodes derived from 1-week-old axenic seedlings on Murashige
and Skoog's medium containing either N
6-benzyladenine (BA), kinetin (Kn), isopentenyladenine (2iP) or thidiazuron (TDZ), with BA being the most effective growth
regulator. High-frequency shoot proliferation (99%) and maximum number of shoots per explant (7.9 shoots) were recorded with
BA at an optimum level of 8.9 μM. Concentrations of all cytokinins tested above the optimum level markedly reduced the frequency of shoot proliferation. A
proliferating shoot culture was established by repeatedly subculturing the original cotyledonary node on shoot multiplication
medium after each harvest of the newly formed shoots. Primary shoots were multiplied as nodal explants, and from each stem
node 2 or 3 shoots developed. Thus, 60–70 shoots were obtained in 3 months from a single cotyledonary node. About 91% of the
shoots developed roots following transfer to half-strength MS medium containing a combination of 5.7 μM indole-3-acetic acid, 4.9 μM indole-3-butyric acid and 5.3 μM indole-3-propionic acid. Eighty percent of the plantlets were successfully acclimatized and established in soil.
Received: 1 October 1997 / Revision received: 31 March 1998 / Accepted: 7 April 1998 相似文献
30.
A highly efficient and reproducible method of in vitro propagation using meristematic explants has been developed for castor.
Embryo axes and shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with 0.5–10.0 mg/l of adenine, N6-benzyladenine (BA), kinetin (Kn), thiadiazuron (TDZ) and zeatin. TDZ (1.0–10.0 mg/l) gave the maximum number of shoots (37.8–40.0)
from embryo axes, while BA (2.0 mg/l) was found superior to other cytokinins for obtaining the highest number of shoots (46.7)
from the shoot apex. Adenine and Kn at all of the tested concentrations resulted in low proliferation rates from embryo axes.
The carryover effect of the cytokinins was tested by subculturing proliferating shoot cultures from various media onto the
medium fortified with 0.5 mg/l BA. There was no significant influence of the cytokinins on subsequent proliferation from the
two explant types except for TDZ with embryo axes. The number of shoots from TDZ-habituated embryo axes ranged between 36.0
and 81.7, while it varied from 5.7 to 22.0 and 3.7 to 28.3 in axillary buds and embryo axes, respectively, on the other media.
For elongation of shoots, gibberellic acid (GA3) (0.1–1.0 mg/l) was added to the medium supplemented with 0.2–0.5 mg/l BA. Incorporation of GA3 (0.1 mg/l) significantly enhanced the frequency of elongated shoots but drastically reduced the multiplication ability. Hence,
proliferating shoot clusters were periodically transferred to the medium supplemented with 0.5 and 0.2 mg/l BA for further
multiplication and elongation. Well-developed shoots were rooted on half-strength MS medium supplemented with 1.0 mg/l indole-3-butyric
acid. The rooted plantlets were acclimatized with more than 60% success.
Received: 17 June 1997 / Revision received: 3 September 1997 / Accepted: 20 September 1997 相似文献