Urban atmospheric environmental capacity and atmospheric environmental carrying capacity constrained by GDP–PM2.5

China faces a contradictory period of economic development and environmental protection, with it being essential to control total emissions within the limit of atmospheric environmental capacity (AEC) by promoting atmospheric environmental carrying capacity (AECC). This implies that well-calculated AEC and AECC values are the key macro-criteria for improving environmental quality and supporting the challenging coordinated development of economy and environment. When considering compound air pollution characterised as fine particulate matter (PM_2.5), conventional methods are not capable of calculating AEC and AECC, but the system dynamics (SD) method retains the advantage of simplicity in resolving complex problems. In the present study, we first describe the background and definitions of AEC and AECC, which are different from Western concepts, and their dialectical relationships. Then, with the statistical data from Wuhan city in 2014, we establish an ‘economy–energy–atmospheric environment’ dynamic model using the SD method, which does not need to simulate the complicated physicochemical processes of atmospheric transmission and diffusion. Instead, it uses the pollutants’ proportionality factors and conversion rates to establish quantitative connections among different types of variables. Finally, we simulate the dynamic trends of gross domestic production (GDP), PM_2.5, and six air pollutant emissions between 2015 and 2030 in four different scenarios and calculate the results of AEC and AECC constrained by GDP and PM_2.5.     

武汉梅花炭疽病病菌的多样性研究

炭疽病是梅花（Prunus mume）栽培中的重要病害,对梅花的栽培构成严重威胁。本研究从武汉发病的梅花叶片样品上分离、获得了170个炭疽病菌菌株,它们在形态特征、致病性、分子遗传水平等方面都表现出较大的差异。按菌落形态、色素分泌、拟菌核产生、分生孢子及孢子梗形态和大小等形态特征将梅树炭疽病菌分为7种类型,其中Ⅵ型和Ⅶ型菌株在PDA培养基上可以连续产生大量的有性后代。7种类型的菌株只能侵染梅花、樱树、梨树、苹果、桃树、杏树等蔷薇科园艺植物,并且存在着明显的致病力分化,但不侵染吉祥草、柑桔、大叶黄杨、豇豆、紫荆、高粱等供试的其它科植物。依据致病力可将梅树炭疽病菌分为强、中、弱3类。ITS序列表明它们均属于胶孢炭疽（Colletotrichum gloeosporioides）。对其中7种类型36个梅树炭疽病菌菌株的进行了RAPD聚类分析,在55%相似水平上,供试菌株可以分为3组,所聚类群与形态学类型和致病力分化所形成的强、中、弱3类没有明显的相关性。表明梅花炭疽病菌菌株间存在丰富的遗传多样性。     

武汉市绿色廊道景观格局

绿色廊道作为城市关键的景观结构要素,在维持城市生物多样性,改善城市生态环境,调节小气候等方面发挥着重要的作用.以武汉市建成区为例,探讨城市绿色廊道景观格局定量研究的方法,使其充分发挥生态功能,从而指导城市绿色廊道的规划与建设.利用GIS处理基础资料,对城市廊道系统进行提取与分类,生成拓扑关系图,并计算景观格局指标参数.从廊道景观的构成和绿色廊道的网络结构两方面详细分析了武汉市建成区各行政区内各类型绿色廊道的景观格局.结果表明：在武汉市建成区内,灰色廊道最长,占廊道总长的63.53﹪;绿色廊道极其缺乏,总密度仅为2.63 km/km^2;在绿色廊道中,绿色道路廊道最长,占绿色廊道总长的60.29﹪.绿色廊道普遍较窄,绿带的长度不到其它类型绿色廊道总长的1/3;绿色道路廊道最窄,少数达20 m.绿色铁路廊道面积较小,为1.68 km^2,其它类型的绿色廊道面积相当,各占总面积的30﹪左右;3个行政区中,武昌的绿色廊道长度最长,面积最大.绿色廊道网络线点率、环通度及连通度均不高,网络结构简单,不利于城市生物多样性的维持.绿色道路廊道网络结构相对最复杂;绿带的网络连通性最差,没有回路存在;武昌的绿色河流廊道、绿带以及绿色铁路廊道网络结构最复杂,绿色道路廊道网络最复杂的是汉口.武汉市绿色廊道的建设,应注重长度和宽度的增加,提高绿带的比例;重视各类型绿色廊道及其在各行政区间的均匀分布;减少绿色廊道的断开区,增加网络连通性;使其走向与城市主导风向一致,兼顾通风走廊的功能.     

武汉东湖浮游生物群落DNA多态性与富营养化

对武汉东湖不同程度富营养化湖区 ,即站 、站 与站 浮游生物进行了群落级 DNA多态性的 DNA指纹研究 ,并就其拓扑结构与富营养化特征参数之间关系进行了分析。实验结果如下 :(1)各站点总氮、总磷及叶绿素 a分别为 1.14 6～ 2 .2 35mg/ L、0 .0 13～ 0 .2 10 mg/ L 和 4 0 .2 5～ 10 9.2 2 μg/ L;(2 )所筛选的 5个随机引物共获得 2 9个扩增位点 ,其中多态位点占75 .9% ,各引物扩增谱带数在 2～ 6间。站 的扩增条带数最多、谱带多态率最高 (6 9.6 % )、特有带最多 ;(3)特定浮游生物类群的特异性 PCR扩增谱带为 1～ 6条不等 ,站间差别甚小。聚类及综合分析表明 :DNA指纹拓扑结构与浮游生物及特定浮游生物类群的物种多样性丰度相吻合 ,并与富营养化主要指示参数存在明显相关 ,即在一定范围内浮游生物群落 DNA多态性与富营养化程度呈反方向发展 ,而原生动物则表现出同向性发展趋势。因此 ,群落级 DNA指纹分析不仅能为生态学研究洞开一片新颖的视窗 ,并有可能孕育出一种简便而灵敏的水体富营养化危机预警系统     

基于“质量-风险-需求”框架的武汉市生态安全格局构建

生态安全格局旨在维护景观格局的整体性与生态过程的连续性,以保障城市生态安全.当前的生态源地识别方法缺乏对生态用地退化风险以及人类生态需求的考虑.本研究以武汉市为例,从生态用地质量、生态退化风险以及生态需求3方面计算生态用地的综合价值以识别生态源地,根据土地利用类型和夜间灯光数据构建基本生态阻力面,基于最小累积阻力模型识别潜在生态廊道,基于电路理论识别生态“夹点”,在此基础上构建了“四横三纵十组团”的武汉市生态安全格局.结果表明: 武汉市生态源地面积为2138.2 km²,占市域面积的24.9%,以水域和林地为主,呈组团形态集中分布在市域南北.生态廊道总长度为1222.42 km,其中,水生廊道566.75 km,陆生廊道为655.67 km,水生廊道贯穿市域呈十字型构架,陆生廊道呈环状分布在市域四周,整体上呈现出“四横三纵”的空间格局,生态廊道上共有生态“夹点”44处,以中心城区为核呈环状分布格局.现有保护空间基本落入生态源地范围,证明了识别框架的生态意义,能够为都市区域生态安全格局的构建提供一个量化框架,以指导相关的城市空间规划.     

基于日常生活视角的公共空间微更新研究—以武汉原俄租界为例

反思武汉原俄租界规划发展中所产生的问题,提出从日常生活视角出发,采用动线观察、PSPL等调研方法,对武汉原俄租界公共空间进行重新解读与分析。总结出弹性设置、微增设计、低技更新3种公共空间微更新策略的同时,认为通过公共空间选点更新的不断累积会完成对公共空间系统的更新,并选取一个公共空间节点进行了具体操作。     

Association of PD-L1 and HIF-1α Coexpression with Poor Prognosis in Hepatocellular Carcinoma

OBJECTIVE: To investigate the correlation between the expression of PD-L1 and HIF-1α in hepatocellular carcinoma (HCC) tissue and further analyze the association with clinical parameters and the prognostic value of coexpression in HCC patients. METHODS: We assessed the expression of PD-L1 and HIF-1α by immunohistochemistry in tumor tissue from 90 HCC patients who underwent curative hepatectomy. The results were validated in an independent cohort of additional 90 HCC patients. RESULTS: PD-L1 and HIF-1α exhibited in tumor tissue high expression rates of 41.11% (37/90) and 43.33% (43/90), respectively, and their expressions were positively correlated (r = 0.563, P < .01). High expression of PD-L1 was significantly associated with low albumin levels (P < .05); high expression of HIF-1α was significantly correlated with high alpha-fetoprotein (AFP) levels and low albumin levels (P < .05); high expression of both PD-L1 and HIF-1α was also significantly associated with high AFP levels and low albumin levels (P < .05). High expression of PD-L1, HIF-1α, as well as both PD-L1 and HIF-1 α was respectively significantly associated with worse overall survival (OS) and disease-free survival (DFS) (P < .05). Patients with co-overexpression of PD-L1 and HIF-1α had the worst prognosis compared with other groups. Additionally, multivariate Cox regression models suggested that high expression of PD-L1, HIF-1α, as well as both PD-L1 and HIF-1α was an independent prognostic factor for OS and DFS (P < .05). Furthermore, the positive correlation and prognostic values of PD-L1 and HIF-1α were validated in an independent data set. CONCLUSION: We demonstrated that HCC patients with co-overexpression of PD-L1 and HIF-1α in tumor tissue had a significantly higher risk of recurrence or metastasis and death compared with others. Therefore, more frequent follow-up is needed for patients with co-overexpression of PD-L1 and HIF-1α. At the same time, a combinational therapy with HIF-1α inhibitors in conjunction with PD-L1 blockade may be beneficial for HCC patients with co-overexpression in the future.     

武汉城市圈县域空间横向碳生态补偿研究——基于土地利用碳收支差异

认识和探讨区域土地利用碳收支并构建碳生态补偿模式,对于引导区域低碳协调发展、促进实现碳中和目标具有重要理论和现实意义。探究基于土地利用碳收支的碳生态补偿原理,并以武汉城市圈县域为实证研究对象,依据区域土地利用碳收支差异,在碳中和目标下估算城市圈各县(市、区)的碳生态补偿标准,明确碳生态补偿支付或受偿次序。结果表明：(1)研究期内城市圈各县(市、区)土地利用净碳排放空间差异显著。土地利用净碳排放呈现“西高东低,南北低中心高”的空间分布特点,且该空间分布特征逐渐强化;(2)武汉城市圈有14个碳生态补偿支付区,大多位于城市圈中部和西部地区,其中包括5个优先支付区、6个次级支付区、3个潜在支付区;(3)武汉城市圈有25个碳生态补偿受偿区,大多位于城市圈东部及外围地区,其中包括5个优先受偿区、9个次级受偿区、11个潜在受偿区,并针对不同类型支付和受偿区提出差别化的低碳发展对策建议。     

漳州水仙在武汉水培开花规律试验初报

通过试验表明,漳州水仙在武汉水培时:(一)在本试验期内的自然气温下,40桩规格的商品鳞茎从水培至始花,最多需时83天,最少47天。水培开始早的需时较长,开始晚的需时较短。(二)开花期的长短,与花期内气温平均值明显相关。平均值升高,花期缩短。(三)在本试验所述的条件下,供春节观赏的水仙,应在节前60—70天开始水培。过早,始花期延长,管理费事。过迟,不能适时开花,而且花期将因气温升高而缩短。(四)40桩规格的商品鳞茎,每个平均有花3.2葶,24.8朵。质量较好,而价格较低,适合子一般群众消费。