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161.
Dina Zand Jeffrey Hammer Robert Gould Richard Quarles 《Journal of neurochemistry》1991,57(3):1076-1079
The major 24- and 28-kDa glycoproteins in shark PNS and CNS myelin express high levels of the adhesion-associated HNK-1/L2 carbohydrate epitope. The 28-kDa protein, but not the 24-kDa protein, cross-reacts strongly with one of two anti-bovine P0 antisera not previously tested against fish myelin proteins. Shark PNS and CNS myelin also contains smaller amounts of high-molecular-weight HNK-1-positive proteins, including a prominent broad band in the 65-85-kDa range. Although myelin-associated glycoprotein (MAG) is well known to react with HNK-1 in some mammals, monoclonal and polyclonal anti-MAG antibodies did not react with the high-molecular-weight HNK-1-positive material in shark myelin, a result suggesting that it is not a MAG-like protein. The high expression of the HNK-1/L2 epitope in glycoproteins of shark myelin, including the major P0-related ones, suggests that this adhesion-related carbohydrate structure may have had an important role in the molecular evolution of the myelinating process. 相似文献
162.
草鱼血液学的研究:Ⅱ.血清电解质和尿素氮的周年变化 总被引:1,自引:0,他引:1
本文研究了草鱼稚鱼血清钾、钠、钙、氯、无机磷和尿素氮含量的周年变动规律,分析了水温、体重对上述血液化学成分的影响。结果显示其周年变动无一定规律性可循。血清钠、氯变动辐度较小,无机磷和尿素氮变动幅度较大,幅值约有4倍。水温与血清钾显示正相关(P≤0.05),与钠、钙显示负相关(P≤0.01,P≤0.05)。血清钠、尿素氮有随体重增加含量增高的趋势,而钾却显著减少。 相似文献
163.
The fish fauna of the stony littoral in the central parts of L. Päijänne was studied by electric fishing on four occasions during 1988–1989. Ten fish species and 1681 individuals (14.5 kg) were caught in the 15 fishing sites (4096 m2) which gives a mean density of 0.41 ind. m–2 and biomass of 3.5 gm–2. Effluent from two large paper mills causes a clear zonation of the fish fauna in the area. In the most polluted shores, only burbot and perch occurred regularly and the reproduction of other species was inhibited. In the semipolluted area (5–15 km from the Kaipola paper mill), burbot and stone loach occurred regularly, but owing to low numbers of perch and bullhead the total densities were usually low.The clean shores were characterized by an abundance of stone loach and bullhead and locally also of minnow, which seemed to be very sensitive to pollution. The strong floods during summer 1988 had positive effects on the reproduction of perch and pike, but the densities of other species were highest during the normal water level in autumn 1989. 相似文献
164.
Modification of an index of biotic integrity based on fish assemblages to characterize rivers of the Seine Basin,France 总被引:14,自引:6,他引:8
The Index of Biotic Integrity (IBI) is a measure of fish assemblage health that has been used to assess catchment and stream quality throughout North America. It reflects human perturbations on natural environmental structures and processes. While preserving the ecological foundation of the original North American metrics, we have modified and adapted the IBI to the mainstem Seine River and its major tributaries in France. This successful modification of the IBI to a considerably different fish fauna on a different continent further supports its wider use outside the midwestern United States. Using data collected in 1967, 1981, and 1988–1989 from a total of 46 sites, we show spatial and temporal variation in the Seine as indicated by IBI scores. Statistically significant relationships were found between IBI and catchment area but insignificant relationships existed between IBI and an independent Water Quality Index (WQI) based on water chemistry. Comparisons between the IBI and the WQI indicate that the former is a more sensitive and robust measure of water body quality. Our results demonstrate that the IBI, combined with a statistically designed national monitoring program, would offer a reliable means of assessing spatial patterns and temporal trends in water body improvement or degradation in France. The more primitive fish families in the Basin were affected first by perturbations. These families include all the diadromous species found in the Seine and suggest serious disruption of their life histories. 相似文献
165.
Jörgen Jensen Susanne Holmgren 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1992,162(2):184-188
Summary The release of substance P-like immunoreactive material (SPLI) from the vascularly perfused stomach of the rainbow trout, Oncorhynchus mykiss, was studied. In most cases, SPLI was detected in the collected vascular perfusate during experimental resting conditions. Distensions of the stomach, accomplished by a water-filled intragastric balloon, produced an initial rapid relaxation of the stomach, followed by a slow further relaxation and a stimulation of contractile activity. The amount of SPLI in the vascular perfusate was significantly elevated during the distension period. Tetrodotoxin had no effect on the response to distension or on the release of SPLI during distension, indicating release from tetrodotoxin-insensitive neurons or endocrine cells. The results suggest that a substance P-like peptide may be involved in the contractile response and/or in the maintenance of muscular tone during gastric distensions in the rainbow trout. Infusion of capsaicin had no effect on the release of SPLI. However, capsaicin caused an increase in vascular flow, an effect that could be repeated on a second infusion of capsaicin, indicating that the action may not be specific to sensory neurons.Abbreviations 5-HT
5-Hydroxytryptamine
- RIA
radioimmunoassay
- SP
substance P
- SPLI
substance P-like immunoreactive material
- TTX
tetrodotoxin 相似文献
166.
Ellen S. Dierenfeld Nancy Katz Jack Pearson Frank Murru Edward D. Asper 《Zoo biology》1991,10(2):119-125
Retinol (n = 17 spp.) and α-tocopherol (n = 9 spp.) concentrations in whole fish utilized for captive animal feeding programs were determined by high-performance liquid chromatography (HPLC) following routine storage and preparation after commercial purchase by two zoological institutions. Vitamin A activity was calculated from retinol values and ranged from 55 IU/100 g (immature herring) to >2,000 IU/100 g (salmon) on an as-fed basis. α-Tocopherol values, a measure of vitamin E activity, ranged from 0.9 IU/100 g (butterfish) to 12.3 IU/100 g (tilapia) on a wet basis. Vitamin levels in whole fish were intermediate to values previously quantified for muscle or liver tissues alone. Vitamin concentrations in fish livers were quantified separately in seven of these species; liver contributed 35–63% of total retinol measured and 8–34% of total α-tocopherol. Based on these analyses, whole fish commonly fed in zoos, aquariums, and marine zoological parks would appear to meet vitamin A requirements established for most species without additional supplementation, whereas levels of vitamin E quantified indicate a need for supplementation of diets for piscivores. 相似文献
167.
Mary Hagedorn Heinrich A. Vischer Walter Heiligenberg 《Developmental neurobiology》1992,23(10):1446-1466
The electric fish, Eigenmannia, will smoothly shift the frequency of its electric organ discharge away from an interfering electric signal. This shift in frequency is called the jamming avoidance response (JAR). In this article, we analyze the behavioral development of the JAR and the anatomical development of structures critical for the performance of the JAR. The JAR first appears when juvenile Eigenmannia are approximately 1 month old, at a total length of 13–18 mm. We have found that the establishment of much of the sensory periphery and of central connections precedes the onset of the JAR. We describe three aspects of the behavioral development of the JAR: (a) the onset and development of the behavior is closely correlated with size, not age; (b) the magnitude (in Hz) of the JAR increases with size until the juveniles display values within the adult range (10–20 Hz) at a total length of 25–30 mm; and (3) the JAR does not require prior experience or exposure to electrical signals. Raised in total electrical isolation from the egg stage, animals tested at a total length of 25 mm performed a correct JAR when first exposed to the stimulus. We examine the development of anatomical areas important for the performance of the JAR: the peripheral electrosensory system (mechano- and electroreceptors and peripheral nerves); and central electrosensory pathways and nuclei [the electrosensory lateral line lobe (ELL), the lateral lemniscus, the torus semicircularis, and the pacemaker nucleus]. The first recognizable structures in the developing electrosensory system are the peripheral neurites of the anterior lateral line nerve. The afferent nerves are established by day 2, which is prior to the formation of receptors in the epidermis. Thus, the neurites wait for their targets. This sequence of events suggests that receptor formation may be induced by innervation of primordial cells within the epidermis. Mechanoreceptors are first formed between day 3 and 4, while electroreceptors are first formed on day 7. Electroreceptor multiplication is observed for the first time at an age of 25 days and correlates with the onset of the JAR. The somata of the anterior lateral line nerve ganglion project afferents out to peripheral electroreceptors and also send axons centrally into the ELL. The first electroreceptive axons invade the ELL by day 6, and presumably a rough somatotopic organization and segmentation within the ELL may arise as early as day 7. Axonal projections from the ELL to the torus develop after day 18. Within the torus semicircularis, giant cells are necessary for the performance of the JAR. Giant cell numbers increase exponentially during development and the onset of the JAR coincides with a minimum of at least 150 giant cells and the attainment of a total length of at least 15 mm and at least 150 giant cells. Pacemaker and relay cells comprise the adult Eigenmannia pacemaker nucleus. The growth and differentiation of these cell types also correlates with the onset of the JAR in developing animals. We describe a gradual improvement of sensory abilities, as opposed to an explosive onset of the mature JAR. We further suggest that this may be a rule common in most developing behavioral systems. © 1992 John Wiley & Sons, Inc. 相似文献
168.
Biomanipulation was carried out in order to improve the water quality of the small hypertrophic Lake Zwemlust (1.5 ha; mean depth 1.5 m). In March 1987 the lake was drained to facilitate the elimination of fish. Fish populations were dominated by planktivorous and benthivorous species (total stock c. 1500 kg) and were collected by seine- and electro-fishing. The lake was subsequently re-stocked with 1500 northern pike fingerlings (Esox lucius L.) and a low density of adult rudd (Scardinius erythrophthalmus). The offspring of the rudd served as food for the predator pike. Stacks of Salix twigs, roots of Nuphar lutea and plantlets of Chara globularis were brought in as refuge and spawning grounds for the pike, as well as shelter for the zooplankton.The impact of this biomanipulation on the light penetration, phytoplankton density, macrophytes, zooplankton and fish communities and on nutrient concentrations was monitored from March 1987 onwards. This paper presents the results in the first year after biomanipulation.The abundance of phytoplankton in the first summer (1987) after this biomanipulation was very low, and consequently accompanied by increase of Secchi-disc transparency and drastic decline of chlorophyll a concentration.The submerged vegetation remained scarce, with only 5 % of the bottom covered by macrophytes at the end of the season.Zooplankters became more abundant and there was a shift from rotifers to cladocerans, comprised mainly of Daphnia and Bosmina species, the former including at least 3 species.The offspring of the stocked rudd was present in the lake from the end of August 1987. Only 19% of the stocked pike survived the first year.Bioassays and experiments with zooplankton community grazing showed that the grazing pressure imposed by the zooplankton community was able to keep chlorophyll a concentrations and algal abundance to low levels, even in the presence of very high concentrations of inorganic N and P. The total nutrient level increased after biomanipulation, probably due to increased release from the sediment by bioturbation, the biomass of chironomids being high.At the end of 1987 Lake Zwemlust was still in an unstable stage. A new fish population dominated by piscivores, intended to control the planktivorous and benthivorous fish, and the submerged macrophytes did not yet stabilize. 相似文献
169.
Oligotrophication as a result of planktivorous fish removal with rotenone in the small,eutrophic, Lake Mosvatn,Norway 总被引:3,自引:1,他引:2
In September 1987 the shallow, eutrophic, Lake Mosvatn was treated with rotenone to eliminate planktivorous fish (mainly whitefish,Coregonus lavaretus, L.), and the effects were studied. The first summer after treatment the zooplankton community changed markedly from rotifer
dominance and few grazers, to a community with few rotifers and many grazers. Accordingly there was a fivefold increase in
the biomass ofDaphnia galeata. Adult females ofD. galeata approximately doubled in weight. The decrease in rotifer biomass was probably mainly due to a loss of food by competition
with the daphnids. The phytoplankton community was also markedly affected. Prior to treatment Secchi depth was 1.7 m and Chl-a 23μg l−1 in the summer. After treatment there was an increase in the proportion of small and gelatinous algae and the mean chlorophyll
concentration fell to 7μg Chl-a l−1. Secchi depth increased to>2.3 m (bottom-sight most of the season). After the treatment there were also fewer cyanobacterial
blooms. This seems to be related to oligotrophication caused indirectly by increased grazing by the zooplankton. Total nutrient
concentrations were affected. Prior to treatment the mean summer concentration of total phosphate was 44μg P l−1. This decreased to 29μg P l−1 in the first summer and 23μg P l−1 the second summer after the treatment. Total nitrogen decreased from 0.68 mg N l−1 before treatment to 0.32 mg N l−1 the first summer after the treatment. The phosphate loading was not reduced, therefor it can be concluded that the fish removal
provided a biomanipulation which caused the more oligotrophic conditions. 相似文献
170.
Enclosures (17 m3) were used in the mesotrophic area of Lake Balaton to determine the impact of benthivorous bream (Abramis brama L.) on the lower trophic levels during summers of 1984–86. In enclosures with a fish biomass similar to the biomass in the
eutrophic area of the lake, the number of phytoplankton species was highest. In enclosures with a low fish biomass the phytoplankton
was dominated by the greens. A high biomass of bream in the mesotrophic basin caused bacterial production corresponding to
that of the eutrophic part of the lake. Crustaceans were dominated by copepods and were unable to control phytoplankton peaks.
Bottom-up effects of bream were more obvious than top-down effects and seem to be more important in the possible control of
water quality. 相似文献