全文获取类型
收费全文 | 1664篇 |
免费 | 159篇 |
国内免费 | 387篇 |
出版年
2024年 | 5篇 |
2023年 | 94篇 |
2022年 | 78篇 |
2021年 | 90篇 |
2020年 | 98篇 |
2019年 | 104篇 |
2018年 | 98篇 |
2017年 | 79篇 |
2016年 | 76篇 |
2015年 | 54篇 |
2014年 | 106篇 |
2013年 | 222篇 |
2012年 | 59篇 |
2011年 | 112篇 |
2010年 | 84篇 |
2009年 | 101篇 |
2008年 | 97篇 |
2007年 | 100篇 |
2006年 | 76篇 |
2005年 | 85篇 |
2004年 | 56篇 |
2003年 | 42篇 |
2002年 | 52篇 |
2001年 | 29篇 |
2000年 | 27篇 |
1999年 | 25篇 |
1998年 | 22篇 |
1997年 | 14篇 |
1996年 | 19篇 |
1995年 | 15篇 |
1994年 | 16篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 8篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1985年 | 8篇 |
1984年 | 6篇 |
1983年 | 2篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有2210条查询结果,搜索用时 14 毫秒
61.
采用水蒸气蒸馏法和气相色谱-质谱联用技术(GC-MS)分析阔叶百里香(Thymus pulegioides)鲜、干茎叶精油含油率、化学组成及其相对含量的差异,并比较精油对链格孢(Alternaria alternata)、粉红单端孢(Trichothecium roseum)和意大利青霉(Penicillium italicum)的抑制效果。鲜、干茎叶含精油率分别为0.53%、0.37%,分别鉴定出35、40种化合物,其主要化学成分相同,均含有百里香酚(鲜样31.30%,干样26.82%)、麝香草酚甲醚、右旋龙脑和邻伞花烃等,只是相对含量略有差异;在抑菌实验中,百里香精油对链格孢菌的抑制作用最为明显,其次是意大利青霉和粉红单端孢。鲜、干阔叶百里香精油的组分较为接近,相对含量略有差异;阔叶百里香精油对3种真菌具有较好的抑制效果,且鲜样精油的抑菌效果好于干样精油。 相似文献
62.
选用产自福建厦门的香茅(Cymbopogon nardus),测定香茅精油对白色粘性沙雷氏菌、红色粘性沙雷氏菌、大肠杆菌、金黄葡萄球菌、铜绿假单胞菌和紫色杆菌等6种菌的抑菌活性,并利用ABTS.+自由基清除法以及超氧阴离子清除法研究香茅精油的抗氧化活性。结果表明,新鲜香茅精油的平均提取率为0.29,对6种供试菌种都有较高的抑制作用,其中对紫色杆菌的抑制活性最强,且香茅精油有极高的清除ABTS.+自由基以及超氧阴离子自由基的能力,证明香茅精油具有广谱抑菌活性及强抗氧化活性。 相似文献
63.
64.
筛选鉴定一株产生抑菌活性物质的海洋放线菌 总被引:1,自引:0,他引:1
目的:分离筛选能够产生抑菌活性物质的海洋放线茵,并进行生理生化和16SrDNA鉴定。方法:用分离培养基培养海洋放线菌,并筛选出能够产生抑菌活性物质的菌株,对所筛选菌株的形态特征、生理生化特性进行鉴定分析;采用通用引物27F、1492R扩增该菌株的16SrDNA,对测序结果进行分析;采用Neighbor—Joining(N—J)法构建系统发育进化树。结果:筛选到一株对金黄色葡萄球菌、大肠杆菌、白色念珠菌具有较强抗性的海洋放线菌F1,该菌株好氧,中度嗜盐,在高氏I号培养基上呈白色绒粉状,16SrDNA序列比对表明该菌株与田无链霉菌(Streptomyces tanashiensis)NR043369的相似度为99%。结论:筛选到的菌株F1是一株海洋来源的放线菌,与田无链霉菌NR043369的同源性较高,可能属海洋链霉菌属,对金黄色葡萄球菌等病原菌具有较强的抑菌活性。 相似文献
65.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(1):67-77
A series of novel hybrid heterocyclic compounds, 3-(3-alkyl-2,6-diarylpiperin-4-ylidene)-2-thioxoimidazolidin-4-ones were synthesised and a comparative study was also carried out under microwave irradiation. The synthesised compounds were characterised by their melting points, elemental analysis, MS, FT-IR, one-dimensional NMR (1H, D2O exchanged 1H and 13C), two dimensional HOMOCOSY and NOESY spectroscopic data. All the synthesised title compounds were screened for their in vitro antibacterial and antifungal activity against clinically isolated strains namely B. subtilis, M. luteus, S. typhii, S. paratyphii B, S. felxneri, P. vulgaris, A. niger, Mucor, Rhizopus and M. gypsuem and the results were discussed. 相似文献
66.
Bashir Ahmad S. M Mukaram Shah Shumaila Bashir Halima Begum 《Journal of enzyme inhibition and medicinal chemistry》2013,28(1):136-139
The crude methanolic extract and subsequent fractions of Teucrium royleanum (Labiatea) were screened for antibacterial and antifungal activities. Against tested pathogens, crude extract and subsequent fractions demonstrated moderate to excellent antibacterial activities. Highest antibacterial activity was displayed by the ethyl acetate fraction against S. typhi (100%), against E.coli (76.7%) and against P. aerugenosa (70.8%) followed by the chloroform fraction against S. typhi (85.7%). Similarly, the crude extract and its subsequent fractions showed mild to excellent activities in the antifungal bioassay with maximum antifungal activity against M. canis (87%) by the chloroform fraction followed by the ethyl acetate (71%) and n-butanol (70%) fractions. 相似文献
67.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(4):468-471
Antibacterial and antifungal activities of the two isolated compounds from Conyza canadensis have been reported in the current study. The two isolated compounds i.e. Conyzolide (1) and Conyzoflavone (2) were tested against six bacterial and five fungal strains, employing hole diffusion and macrodilution methods. Both the compounds showed significant activities against the tested pathogens with special reference to E. coli, P. aeruginosa, S. aureus, Trichophytom longifusus, C. albicans, and C. glaberata. Conyzolide revealed comparatively better antibacterial activity against E. coli (minimum inhibitory concentration (MIC): 25 µg/mL) in comparison to Conyzoflavone. However, in case of antifungal activities, Conyzoflavone exhibited superior antifungal activity against C. albicans (MIC: 10 µg/mL) as compared to Conyzolide. 相似文献
68.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):649-656
Direct interaction between 7-chloro-1-cyclopropyl-6-fluoro-8-nitro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid and primary α-amino acids (exemplified by glycine, alanine, and l-valine) in aqueous ethanolic NaHCO3 at 70–80°C for 24–72?h produced the respective N-(4-oxoquinolin-7-yl)-α-amino acids (6a–c). The latter derivatives underwent reductive lactamization upon treatment with Na2S2O4 in aqueous ethanol to afford moderate yields of the corresponding pyrido[2,3-f]quinoxaline-8-carboxylic acids (8a–c). Acetylation of 8a–c using acetyl chloride afforded N4-acetylated hexahydro-2,7-dioxopyrido[2,3-f]quinoxaline-8-carboxylic acids (9a–c). The structures, assigned to these new heterocyclic products, are supported by analytical and spectral data. The synthesized compounds (6a–c/9a–c) showed appreciable antibacterial activity as compared with ciprofloxacin. 相似文献
69.
Zahid H. Chohan Claudiu T. Supuran 《Journal of enzyme inhibition and medicinal chemistry》2013,28(2):240-251
Cobalt (II), copper (II), nickel (II) and zinc (II) complexes with 2-hydroxy-1-naphthaldehyde derived N-substituted sulfonamides have been synthesized and the nature of bonding and structure of compounds have been deduced from physical, analytical and spectral (IR, 1H NMR, 13C NMR, Mass and electronic) data. An octahedral geometry has been suggested for the complexes. Complexes along with the ligands were assessed for their antibacterial and antifungal activities on different species of pathogenic bacteria and fungi. The results revealed the ligands to possess moderate to significant antibacterial activity which was, in many cases, enhanced on chelation. Similar results were observed for antifungal activity. Brine shrimp bioassay was also carried out for in vitro cytotoxic properties against Artemia salina. 相似文献
70.
Muhammad Nisar Inamullah Khan Bashir Ahmad Ihsan Ali Waqar Ahmad Muhammad Iqbal Choudhary 《Journal of enzyme inhibition and medicinal chemistry》2013,28(2):256-260
Current study was undertaken to evaluate the in vitro antifungal and antibacterial potential of methanol extract and subsequent fractions obtained after partitioning in organic solvents with variable polarity of the aerial parts of the tree Taxus wallichiana Zucc. Traditionally, this plant is often used in folk medicines in Pakistan for treating microbial infections. In order to rationalize the traditional use, methanol extracts of leaf, bark, and heartwood of Taxus wallichiana Zucc. were tested against six bacteria and six fungal strains using the Hole diffusion and macro-dilution methods. All extracts and fractions displayed significant antimicrobial effect. Only three fungal strains, Trichophyton longifusus, Microspoum canis, and Fusarium solani were susceptible to the extracts and fractions with MICs ranging from 0.08 to 200 mg/mL. In case of bacterial strains, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi were susceptible to the extracts and fractions with MICs ranging from 0.08 to 200 mg/mL. Comparison results were carried out using imipinem, miconazole and amphotericin B as standard antibiotics. 相似文献